FastQCFastQC Report
Thu 26 May 2016
SRR936227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006198
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG29800.058.893722
GGTATCA32950.058.4995841
GTACATG163900.052.196711
TACATGG166700.050.6419032
ACATGGG170500.047.8034673
CATGGGA116200.043.3139154
GTACCTG41750.042.60671
CATGGGG55600.041.9444474
GTATCAA46750.040.8494872
ACCTGGG43950.039.9324873
TCAACGC51700.037.1685145
ATGGGAT48550.036.516595
ATCAACG52850.036.4723054
CAACGCA54400.035.6518336
ATGGGAG44400.035.1060145
AACGCAG56400.034.0719877
GAGTACA98400.032.829781
AGTACAT95650.031.9699082
TATCAAC60750.031.5335433
ATGGGGG34900.031.3657445
Produced by FastQC (version 0.11.5)