##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936227_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2006198 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.67055345484344 31.0 30.0 31.0 22.0 34.0 2 28.544430808923146 31.0 28.0 33.0 18.0 34.0 3 28.8134560995475 31.0 30.0 34.0 22.0 34.0 4 32.232328513935315 35.0 33.0 37.0 20.0 37.0 5 32.44536431598476 35.0 33.0 37.0 25.0 37.0 6 32.27325916983269 35.0 32.0 37.0 23.0 37.0 7 32.21992694639313 35.0 33.0 37.0 22.0 37.0 8 32.1859656923195 35.0 33.0 37.0 21.0 37.0 9 33.50848869353872 37.0 33.0 39.0 18.0 39.0 10-11 33.29534472669198 37.0 33.0 39.0 17.0 39.0 12-13 33.28342840537175 37.0 33.0 39.0 17.0 39.0 14-15 34.25250947314273 38.0 33.0 40.0 17.0 41.0 16-17 34.23092037774936 38.0 33.0 40.0 17.0 41.0 18-19 34.231978099868506 38.5 33.0 40.0 18.0 41.0 20-21 34.360083351693106 39.0 33.0 40.0 19.0 41.0 22-23 34.21914312545422 38.5 33.0 40.0 19.5 41.0 24-25 34.19382209532658 38.0 33.0 40.0 20.5 41.0 26-27 34.29794666329046 38.0 33.0 40.0 22.5 41.0 28-29 34.173819333884296 38.0 33.0 40.0 22.0 41.0 30-31 34.09194830221145 38.0 33.0 40.0 22.0 41.0 32-33 33.93574936272491 38.0 32.5 40.0 20.0 41.0 34-35 33.87617697754658 38.0 32.0 40.0 19.0 41.0 36-37 33.742674701101286 38.0 32.0 40.0 19.0 41.0 38-39 33.477575992000794 38.0 32.0 40.0 17.0 41.0 40-41 33.55927231509552 38.0 32.0 40.0 18.5 41.0 42-43 33.43739551131045 38.0 32.0 40.0 16.0 41.0 44-45 33.25193001887152 38.0 32.0 40.0 15.0 41.0 46-47 33.20645021079674 38.0 32.0 40.0 15.0 41.0 48-49 33.10299332368989 38.0 31.5 40.0 14.5 41.0 50-51 32.94155013612814 38.0 31.0 40.0 13.5 41.0 52-53 32.780576991902095 37.5 31.0 40.0 10.5 41.0 54-55 32.59850548151279 37.0 31.0 40.0 8.5 41.0 56-57 32.387474217400275 37.0 31.0 40.0 8.0 41.0 58-59 32.146375382689044 37.0 30.0 40.0 7.0 41.0 60-61 31.882556955993376 36.0 30.0 40.0 4.0 41.0 62-63 31.6320435470477 36.0 30.0 39.5 2.0 41.0 64-65 31.344684821737435 35.5 30.0 39.0 2.0 41.0 66-67 31.04945125057447 35.0 30.0 39.0 2.0 40.0 68-69 30.765330490808985 35.0 29.0 39.0 2.0 40.0 70-71 30.42230328212868 35.0 29.0 38.0 2.0 40.0 72-73 30.09963747346972 34.5 29.0 37.5 2.0 40.0 74-75 29.76189887538518 34.0 29.0 37.0 2.0 39.0 76-77 29.43629990658948 34.0 29.0 36.5 2.0 39.0 78-79 29.096548795283418 34.0 28.5 36.0 2.0 38.5 80-81 28.785274185299755 34.0 28.0 35.5 2.0 37.5 82-83 28.482576495440632 34.0 28.0 35.0 2.0 37.0 84-85 28.51181687949046 34.0 28.5 35.0 2.0 37.0 86-87 28.386474814549707 34.0 29.0 35.0 2.0 36.0 88-89 28.20559162156477 34.0 28.0 35.0 2.0 36.0 90-91 28.025463588339733 34.0 28.0 35.0 2.0 36.0 92-93 27.897268863791112 34.0 28.0 35.0 2.0 35.0 94-95 27.76183033778321 34.0 27.0 35.0 2.0 35.0 96-97 27.6457625319136 34.0 27.0 35.0 2.0 35.0 98-99 27.525507203177355 34.0 27.0 35.0 2.0 35.0 100-101 27.400172365838266 34.0 27.0 35.0 2.0 35.0 102-103 26.430248659404505 32.0 25.0 34.0 2.0 35.0 104-105 27.087984336541062 33.0 25.5 35.0 2.0 35.0 106-107 27.206278243722704 34.0 26.0 35.0 2.0 35.0 108-109 27.151660005642515 34.0 26.0 35.0 2.0 35.0 110-111 27.022425752592714 34.0 25.0 35.0 2.0 35.0 112-113 26.974425256131248 34.0 25.0 35.0 2.0 35.0 114-115 26.84927285342723 34.0 25.0 35.0 2.0 35.0 116-117 26.695056519845004 34.0 25.0 35.0 2.0 35.0 118-119 26.461872905864723 33.5 25.0 35.0 2.0 35.0 120-121 26.22676600215931 33.0 25.0 35.0 2.0 35.0 122-123 25.86859746645147 33.0 23.5 35.0 2.0 35.0 124-125 23.857994076357368 31.0 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7912178970923271 1101 2 0.8649259657760631 1101 3 0.7923087487040945 1101 4 0.850949589318752 1101 5 0.8175429543152575 1101 6 0.7235676541024816 1101 7 0.6542759746536504 1101 8 0.8116424958277051 1101 9 0.9836051131984433 1101 10-11 0.9269562682582375 1101 12-13 0.8453007084889279 1101 14-15 1.086425150967223 1101 16-17 1.1159468708087203 1101 18-19 0.9546202690718317 1101 20-21 0.8215687051167606 1101 22-23 1.0081443179894762 1101 24-25 1.0775049530011813 1101 26-27 0.9700385278179837 1101 28-29 1.0658655833698134 1101 30-31 1.1096733254773028 1101 32-33 1.1777854480191579 1101 34-35 1.036440219533219 1101 36-37 0.977951386860525 1101 38-39 1.1484525251083681 1101 40-41 1.1157319739070672 1101 42-43 1.3200700847107214 1101 44-45 1.1945208583773947 1101 46-47 1.17494778775783 1101 48-49 1.0647709930411295 1101 50-51 1.085381088501748 1101 52-53 1.113905838863758 1101 54-55 1.0869924521687864 1101 56-57 1.178918239798449 1101 58-59 1.244474206434603 1101 60-61 1.1713632389736937 1101 62-63 1.2647753764932297 1101 64-65 1.2958891781811772 1101 66-67 1.3084982485650123 1101 68-69 1.281592699172073 1101 70-71 1.1414826648818241 1101 72-73 1.1041366128874621 1101 74-75 1.1615479866195209 1101 76-77 1.1002002338739274 1101 78-79 1.141108050837321 1101 80-81 1.1791646237792648 1101 82-83 1.0615786165120333 1101 84-85 0.8282894602060438 1101 86-87 0.9030822383431776 1101 88-89 0.8329700577770538 1101 90-91 0.8422663952029659 1101 92-93 0.8929901329483343 1101 94-95 0.9448134232248826 1101 96-97 0.9265590487970172 1101 98-99 0.925255592210803 1101 100-101 0.95092573066961 1101 102-103 0.9364260336021744 1101 104-105 0.8919569094560416 1101 106-107 0.8710118633411845 1101 108-109 0.8470119977822712 1101 110-111 0.8952677978890549 1101 112-113 0.9431675001988182 1101 114-115 0.9818154301916842 1101 116-117 1.0628873156861225 1101 118-119 1.0488761012025165 1101 120-121 1.1260066414206626 1101 122-123 1.0628664947209643 1101 124-125 0.805913406961615 1102 1 0.5631373434373188 1102 2 0.5265770008517769 1102 3 0.6964149426084809 1102 4 0.7645326244509789 1102 5 0.6946144680870034 1102 6 0.6000369126463738 1102 7 0.5879219398060016 1102 8 0.6271618703218707 1102 9 0.8218061380818398 1102 10-11 0.9188782980036692 1102 12-13 0.7762741660676724 1102 14-15 0.8405887649829822 1102 16-17 0.9608244721214092 1102 18-19 0.7753929278147282 1102 20-21 0.8338840575793682 1102 22-23 0.9217174635418957 1102 24-25 1.0478407546928992 1102 26-27 0.8468678477983715 1102 28-29 0.9078765184194992 1102 30-31 0.9037522927629738 1102 32-33 1.051321134265173 1102 34-35 0.9999995398567094 1102 36-37 0.9366060810807681 1102 38-39 0.8745209529275542 1102 40-41 0.9260590162566729 1102 42-43 1.137191268392506 1102 44-45 1.0537174612058209 1102 46-47 0.8908971571457514 1102 48-49 0.9030981605241877 1102 50-51 0.8613795828636768 1102 52-53 0.9334451257241412 1102 54-55 1.0514826039637413 1102 56-57 1.0527266968019902 1102 58-59 1.1677057376443045 1102 60-61 1.1493413648377633 1102 62-63 1.098805723375289 1102 64-65 0.9831496838414537 1102 66-67 0.9466270189673232 1102 68-69 0.9761011567810982 1102 70-71 0.9813905505098433 1102 72-73 0.9320961705608681 1102 74-75 0.8631154850200353 1102 76-77 0.8758968942233736 1102 78-79 0.9816329251488334 1102 80-81 0.8444082000935182 1102 82-83 0.8608323568923986 1102 84-85 0.9464178673176633 1102 86-87 0.9485482696547969 1102 88-89 0.9185569034249355 1102 90-91 0.8982719939161079 1102 92-93 0.9715281301267744 1102 94-95 0.9309039310447176 1102 96-97 0.9643055566652876 1102 98-99 0.9929827649413987 1102 100-101 1.0103589770150023 1102 102-103 1.0230420010351793 1102 104-105 1.0498274003626769 1102 106-107 0.9410282719705485 1102 108-109 0.9411514126017089 1102 110-111 1.0760485095755143 1102 112-113 0.991438236528495 1102 114-115 1.0309407069485523 1102 116-117 1.002272668209276 1102 118-119 1.0935753873363154 1102 120-121 1.039647601234659 1102 122-123 1.146770497175197 1102 124-125 1.0601065888027321 1103 1 0.30376973385475026 1103 2 0.42934732312881607 1103 3 0.4359749373442092 1103 4 0.42125912566535106 1103 5 0.35919071426342697 1103 6 0.3608039407663526 1103 7 0.439279084657187 1103 8 0.4052054760280228 1103 9 0.497135852893976 1103 10-11 0.5940126964212027 1103 12-13 0.5023089740613855 1103 14-15 0.6047603203754619 1103 16-17 0.4599946516250597 1103 18-19 0.5149098512475021 1103 20-21 0.3873047365904796 1103 22-23 0.5829706390534639 1103 24-25 0.6730805382197218 1103 26-27 0.5849165582515354 1103 28-29 0.6835577828015289 1103 30-31 0.6903591115883216 1103 32-33 0.8312743341040587 1103 34-35 0.6906348216347595 1103 36-37 0.6451990054228389 1103 38-39 0.7678583607529745 1103 40-41 0.72813999828238 1103 42-43 0.8531511801761056 1103 44-45 0.7398089199271567 1103 46-47 0.5825752230847243 1103 48-49 0.5160617253923689 1103 50-51 0.49684082467314283 1103 52-53 0.4378822675443814 1103 54-55 0.4826401053496028 1103 56-57 0.38804626194135494 1103 58-59 0.291347196963855 1103 60-61 0.39490970663248604 1103 62-63 0.37492288529547224 1103 64-65 0.3147835618140675 1103 66-67 0.25170155624991963 1103 68-69 0.26804891687393706 1103 70-71 0.20981751075263588 1103 72-73 0.3154845062139344 1103 74-75 0.20843786175833756 1103 76-77 0.15174904798792355 1103 78-79 0.2085114122294378 1103 80-81 0.15433444048722222 1103 82-83 0.13735371108606742 1103 84-85 0.14812157663422454 1103 86-87 0.07209182152181626 1103 88-89 0.10316738729911634 1103 90-91 0.10509131777641656 1103 92-93 0.08945237249059446 1103 94-95 0.23892805496245018 1103 96-97 0.25603945577475073 1103 98-99 0.15005313866471326 1103 100-101 0.22062410481314387 1103 102-103 0.21614790060726108 1103 104-105 0.3150553991024374 1103 106-107 0.3394793999999983 1103 108-109 0.23237742926392357 1103 110-111 0.21101343438019882 1103 112-113 0.302649836268472 1103 114-115 0.3438904132261946 1103 116-117 0.32702620464241505 1103 118-119 0.312182589017695 1103 120-121 0.16654214035177617 1103 122-123 0.22667723584006438 1103 124-125 0.14727582711864784 1104 1 0.4138770572581727 1104 2 0.3499938461980854 1104 3 0.4300248813071086 1104 4 0.5085550446020619 1104 5 0.49151240388962947 1104 6 0.3056835207830275 1104 7 0.2188914641535007 1104 8 0.3378880190761535 1104 9 0.520235812560017 1104 10-11 0.5240563942002794 1104 12-13 0.5801650726994367 1104 14-15 0.6944308413531104 1104 16-17 0.7271260223579077 1104 18-19 0.6652277800907527 1104 20-21 0.6860779385807163 1104 22-23 0.6823621622263616 1104 24-25 0.732922861136899 1104 26-27 0.6451879276771209 1104 28-29 0.7223200324480104 1104 30-31 0.6870305635949023 1104 32-33 0.9083753666852843 1104 34-35 0.883441108630798 1104 36-37 0.7831289885399713 1104 38-39 0.9089871615908933 1104 40-41 0.7011447427037822 1104 42-43 0.881012499909609 1104 44-45 0.7527723818243146 1104 46-47 0.7478780944480903 1104 48-49 0.6635104430753742 1104 50-51 0.8013123940148716 1104 52-53 0.7517470260268695 1104 54-55 0.7897463351128735 1104 56-57 0.7690517173882725 1104 58-59 0.7889014525153542 1104 60-61 0.8703398691002242 1104 62-63 0.7407104417111974 1104 64-65 0.6741659102070088 1104 66-67 0.6878254388956364 1104 68-69 0.7407898166241225 1104 70-71 0.8200577528839261 1104 72-73 0.9032457403374217 1104 74-75 0.8348367964954413 1104 76-77 0.703576989377737 1104 78-79 0.5629335206592323 1104 80-81 0.6649623589201568 1104 82-83 0.7187649373181308 1104 84-85 0.6595651417735695 1104 86-87 0.6814831374451238 1104 88-89 0.6205737622965302 1104 90-91 0.5870197513042967 1104 92-93 0.5211170012029491 1104 94-95 0.46940873053234355 1104 96-97 0.5005029025267511 1104 98-99 0.5260879916689554 1104 100-101 0.47985468273660103 1104 102-103 0.4392371390925547 1104 104-105 0.44682389174428394 1104 106-107 0.589914424861977 1104 108-109 0.6231670581755573 1104 110-111 0.6388869898106933 1104 112-113 0.5858924713354199 1104 114-115 0.48752195215005756 1104 116-117 0.49759421200137055 1104 118-119 0.49204394701266807 1104 120-121 0.5189987654037864 1104 122-123 0.5227346710785525 1104 124-125 0.38915204172081275 1105 1 0.4012828636858998 1105 2 0.5575113340859374 1105 3 0.5499975812195999 1105 4 0.43961877920047243 1105 5 0.32146145055261144 1105 6 0.3732963305574941 1105 7 0.4567430827430812 1105 8 0.4565727077913522 1105 9 0.3951049635399002 1105 10-11 0.6518572918107708 1105 12-13 0.5748598292080231 1105 14-15 0.650455429547911 1105 16-17 0.6483123789304486 1105 18-19 0.5094336594552402 1105 20-21 0.4082262963615193 1105 22-23 0.37869427417089696 1105 24-25 0.5124838109846053 1105 26-27 0.3927269027659932 1105 28-29 0.5634471692548431 1105 30-31 0.5878368652994652 1105 32-33 0.6747491179583349 1105 34-35 0.5808635306195811 1105 36-37 0.5714376236694889 1105 38-39 0.6188148386496053 1105 40-41 0.519485246313991 1105 42-43 0.6832297262175686 1105 44-45 0.7045404878236425 1105 46-47 0.609197135274556 1105 48-49 0.6037365627846114 1105 50-51 0.5276725346268591 1105 52-53 0.49542452739935783 1105 54-55 0.5489407186672892 1105 56-57 0.6023605021720542 1105 58-59 0.5360458129521248 1105 60-61 0.5933189064767639 1105 62-63 0.49822741762395495 1105 64-65 0.5245161799391198 1105 66-67 0.8233610002920848 1105 68-69 0.6918971793773849 1105 70-71 0.6138196119994106 1105 72-73 0.5678809817100507 1105 74-75 0.5757325993235938 1105 76-77 0.5763716566451222 1105 78-79 0.6131656619832633 1105 80-81 0.5195377031446498 1105 82-83 0.4664833006046294 1105 84-85 0.49799515438390785 1105 86-87 0.6365277856118965 1105 88-89 0.5925917633409554 1105 90-91 0.6377554989021483 1105 92-93 0.6532065763660171 1105 94-95 0.6188163556531521 1105 96-97 0.550216767521281 1105 98-99 0.5380035228667488 1105 100-101 0.5452890384710756 1105 102-103 0.5724597545291559 1105 104-105 0.5272995835604775 1105 106-107 0.5627553274189374 1105 108-109 0.502724818024582 1105 110-111 0.6641742275317597 1105 112-113 0.6059733161415934 1105 114-115 0.5718249531905464 1105 116-117 0.5269631711875 1105 118-119 0.6270158435180804 1105 120-121 0.6177332614194384 1105 122-123 0.7329459356334169 1105 124-125 0.7900300199390315 1106 1 -0.33419496267262616 1106 2 -0.3819015328722166 1106 3 -0.21452459242187416 1106 4 -0.1066023326046519 1106 5 -0.23912568248923094 1106 6 -0.14700348205961689 1106 7 -0.2615579790932685 1106 8 -0.2520469049506815 1106 9 -0.13550090779051516 1106 10-11 -0.05988536902620467 1106 12-13 -0.10955616079822761 1106 14-15 -0.16512858044583822 1106 16-17 -0.12011360482970446 1106 18-19 -0.12860506634113733 1106 20-21 -0.18562129889144785 1106 22-23 -0.28386037917076123 1106 24-25 -4.1616261203083305 1106 26-27 -1.1889408049167045 1106 28-29 -0.754322974405369 1106 30-31 -0.8125379004719306 1106 32-33 -5.662540076295258 1106 34-35 -1.410235782309833 1106 36-37 -0.48477724203944206 1106 38-39 -0.10245311887693731 1106 40-41 -0.6858551659283592 1106 42-43 -0.2777321725956696 1106 44-45 -0.28749573953425767 1106 46-47 -0.40173353305772963 1106 48-49 -0.12752514864575204 1106 50-51 -0.09318942664734209 1106 52-53 -0.008635435124070057 1106 54-55 -0.2235452276162775 1106 56-57 0.042866435963432536 1106 58-59 0.08632688728066995 1106 60-61 0.05037699517133376 1106 62-63 0.04148163373887925 1106 64-65 0.09010018993287261 1106 66-67 0.025266059629995397 1106 68-69 0.056044587247463085 1106 70-71 0.04105259138729167 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-1.485489768031421 2216 80-81 -1.6570754074942187 2216 82-83 -1.5599926211093837 2216 84-85 -1.8262932706635304 2216 86-87 -1.6403757033128379 2216 88-89 -1.5162912639889754 2216 90-91 -1.405374921245599 2216 92-93 -1.3511105783534596 2216 94-95 -1.4349243092775659 2216 96-97 -1.6731934706244722 2216 98-99 -1.5658847609027653 2216 100-101 -1.495258200149948 2216 102-103 -1.5155677117010384 2216 104-105 -1.5939080463956579 2216 106-107 -1.8298026919404045 2216 108-109 -1.8530973258679317 2216 110-111 -1.7166759171240358 2216 112-113 -1.833272494410874 2216 114-115 -1.897428312379951 2216 116-117 -1.733223773167719 2216 118-119 -1.820677960283387 2216 120-121 -1.842043597683091 2216 122-123 -1.6925506408658535 2216 124-125 -1.712609637201254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87553.0 3 13981.0 4 8960.0 5 7293.0 6 7871.0 7 9201.0 8 9976.0 9 11067.0 10 12500.0 11 13518.0 12 14549.0 13 14782.0 14 14629.0 15 13952.0 16 13093.0 17 11866.0 18 10632.0 19 9987.0 20 9711.0 21 10063.0 22 11810.0 23 15562.0 24 22335.0 25 31089.0 26 35922.0 27 36278.0 28 42101.0 29 49501.0 30 57970.0 31 70282.0 32 87198.0 33 111494.0 34 148776.0 35 205580.0 36 311586.0 37 364035.0 38 99275.0 39 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.473859719518785 17.078848267491445 9.00999155117025 22.437300461819515 2 19.29176482082028 20.230356126364395 37.680577889121615 22.797301163693714 3 17.24166807064906 28.972514178560644 30.082773484970076 23.70304426582022 4 10.6733340112332 15.815553415518725 36.80786922115287 36.703243352095214 5 10.701610619963073 42.27393898067176 34.57165444445774 12.452795954907426 6 30.512602470149947 36.668338156728616 19.70751582349464 13.111543549626806 7 28.940429371096453 34.289125157637116 21.04890364322422 15.72154182804221 8 28.40628877084248 33.54595975732973 19.5631097723583 18.48464169946949 9 28.924382741434044 13.532508976063331 18.167711693282072 39.37539658922055 10-11 24.321457499968847 27.017433673532963 28.926688848181243 19.734419978316946 12-13 25.004299243283334 25.982034396937042 28.105785809456542 20.90788055032308 14-15 22.81830301365725 27.38049812314679 25.171017827426205 24.63018103576975 16-17 22.794435235301698 29.471200804921445 25.77318791112467 21.961176048652188 18-19 22.13189748656199 28.471806661145326 27.21609704925812 22.18019880303456 20-21 23.607357376068588 27.681753607656457 26.860030406500012 21.850858609774942 22-23 23.31041646761283 27.971332255802416 26.51778811624262 22.20046316034214 24-25 22.6825072071751 27.803657783113568 27.04813498222101 22.465700027490325 26-27 22.563676571343404 27.827902222813726 26.834618242141072 22.773802963701794 28-29 22.54466026991185 27.924927729331483 26.916739753565682 22.613672247190983 30-31 22.810252724208556 27.60698322532536 27.198794324933118 22.383969725532964 32-33 22.414689475622964 27.905223433097177 27.211837874239038 22.46824921704082 34-35 22.780648624776504 28.023290995886217 26.945473070004084 22.2505873093332 36-37 22.832132382408826 27.776260250784073 26.77126969157427 22.62033767523283 38-39 22.65969754503951 27.708364590867333 27.019593925379688 22.612343938713465 40-41 22.697460242770326 27.64022199448104 27.206336831863204 22.455980930885424 42-43 22.415852264060845 27.625345558261643 27.223137175539108 22.7356650021384 44-45 22.28674524574009 27.801519758126723 27.304005708376817 22.607729287756374 46-47 22.5506063793198 27.729803556029648 26.778837286969697 22.940752777680853 48-49 22.448496394542005 27.76986478629066 26.983101588604697 22.79853723056264 50-51 22.424727441660153 27.622302330403713 27.18485317340054 22.768117054535594 52-53 22.55866627986151 27.522059358641158 27.2372391682094 22.682035193287934 54-55 22.222133606955126 27.74674997277157 27.30336902230139 22.72774739797191 56-57 22.544933699801316 27.98281109652284 26.933203334898497 22.539051868777346 58-59 22.648143629717378 27.617372562321595 26.808649146029044 22.92583466193198 60-61 22.595561926959913 27.77853100360585 27.268558349026463 22.35734872040778 62-63 22.383787077663868 27.744902931071653 27.284599692699175 22.586710298565308 64-65 22.30976263648732 27.716468749509332 27.26209877506281 22.711669838940537 66-67 22.424227168459282 27.95931001687773 26.954322463331064 22.662140351331924 68-69 22.453364476850716 27.715985875756903 27.11761462987664 22.713035017515736 70-71 22.28039218618376 27.485083666053566 27.430552440197587 22.803971707565086 72-73 22.349555489704024 27.7270197610254 27.035213150855363 22.88821159841521 74-75 22.19908832391667 27.980530307372913 27.05113411208781 22.769247256622613 76-77 22.00449657298931 27.879275912679454 27.314774315252084 22.801453199079152 78-79 22.355991139449984 27.731263545535928 27.15268099427972 22.760064320734365 80-81 22.34840375487397 27.68951633504761 26.995240985099883 22.966838924978536 82-83 22.37388012589993 27.6857974779639 27.198307842726273 22.7420145534099 84-85 22.157608426106453 27.75265695956048 27.141948171998365 22.9477864423347 86-87 21.955977338183647 27.796564041164512 27.30942271784284 22.938035902809002 88-89 22.25873140289379 27.642611563945135 27.347575070687384 22.75108196247369 90-91 22.292105775387185 27.68074082547829 27.226871572023548 22.80028182711098 92-93 22.177469200417107 27.661903423194943 27.226626909833335 22.934000466554615 94-95 22.023119399220615 27.466461369139772 27.36385414371691 23.146565087922706 96-97 22.15663360152807 27.55412431963748 27.38168315437484 22.907558924459604 98-99 21.99676999685972 27.60062606233707 27.120686475358763 23.281917465444447 100-101 21.862599323793198 27.667928926009537 27.341215268137482 23.128256482059783 102-103 21.74259906194928 27.525276252422827 27.427777596393945 23.30434708923395 104-105 21.728799365962345 27.540686575050223 27.358749669772052 23.37176438921538 106-107 21.482885469471984 27.724044083561378 27.532985410155568 23.26008503681107 108-109 21.72988729623363 27.77266443307795 27.145505021088496 23.351943249599923 110-111 21.69893190255729 27.94177901927241 27.148282510477607 23.211006567692692 112-113 21.548355903035226 27.69021417436263 27.44320397967792 23.31822594292422 114-115 21.36167493523797 27.990131547793805 27.19297414852325 23.45521936844498 116-117 21.29925173918364 27.912722926170087 27.43584778177292 23.35217755287335 118-119 21.346037091424588 27.703174892756277 27.321204798377813 23.629583217441326 120-121 21.31487677126969 27.822741437990857 27.39758188165815 23.4647999090813 122-123 21.432678367639298 27.644588377002837 27.230544119402655 23.692189135955207 124-125 21.450553361516064 27.61598709595737 26.90698252860019 24.026477013926378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 33.0 1 46.0 2 72.0 3 91.0 4 91.0 5 94.0 6 115.0 7 124.5 8 127.0 9 147.0 10 159.5 11 171.5 12 203.5 13 231.0 14 287.0 15 353.5 16 440.5 17 571.5 18 774.5 19 979.5 20 1224.0 21 1781.0 22 2555.0 23 3637.0 24 5189.5 25 7278.0 26 10390.5 27 13649.5 28 15899.5 29 18908.0 30 24342.0 31 29820.5 32 34449.0 33 39180.5 34 45379.0 35 50103.0 36 54506.5 37 60032.5 38 61738.0 39 61882.5 40 62544.0 41 64316.0 42 65932.0 43 69126.0 44 73489.0 45 75536.0 46 76079.5 47 76253.5 48 76233.5 49 74745.0 50 74257.0 51 71855.5 52 68723.5 53 66799.0 54 62718.5 55 57867.5 56 52836.5 57 48025.0 58 43749.5 59 38997.5 60 33178.5 61 27513.0 62 22734.5 63 17760.5 64 13313.0 65 10450.5 66 8085.5 67 6470.5 68 4954.5 69 3391.0 70 2510.0 71 1970.0 72 1626.5 73 1361.0 74 1056.0 75 801.5 76 546.5 77 371.5 78 303.5 79 207.0 80 116.0 81 81.5 82 51.5 83 40.5 84 39.0 85 24.0 86 13.5 87 17.0 88 15.5 89 9.5 90 10.0 91 7.5 92 6.0 93 6.0 94 5.5 95 4.5 96 4.0 97 3.5 98 3.5 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4953658611961533E-4 2 0.0 3 0.0 4 9.969105741307687E-5 5 6.978374018915381E-4 6 1.9938211482615375E-4 7 3.987642296523075E-4 8 9.470650454242303E-4 9 0.0014455203324896147 10-11 0.001146447160250384 12-13 0.0015452113899026916 14-15 0.0023676626135605756 16-17 0.001968898383908268 18-19 0.002068589441321345 20-21 0.002392585377913845 22-23 0.09592771999573321 24-25 0.0018692073264951915 26-27 0.0012212154533101918 28-29 0.0012212154533101918 30-31 9.470650454242303E-4 32-33 0.001345829275076538 34-35 7.476829305980766E-4 36-37 4.984552870653843E-4 38-39 7.476829305980766E-4 40-41 0.0010966016315438456 42-43 0.0011962926889569226 44-45 0.0011713699246036534 46-47 0.0013956748037830763 48-49 1.7445935047288454E-4 50-51 0.0010966016315438456 52-53 9.969105741307687E-4 54-55 0.0011215243958971148 56-57 9.969105741307687E-4 58-59 5.73223580125192E-4 60-61 5.981463444784613E-4 62-63 0.001021833338484038 64-65 6.72914637538269E-4 66-67 1.9938211482615375E-4 68-69 9.969105741307687E-5 70-71 3.987642296523075E-4 72-73 7.476829305980766E-5 74-75 1.4953658611961533E-4 76-77 1.7445935047288454E-4 78-79 9.969105741307687E-5 80-81 2.741504078859614E-4 82-83 7.476829305980766E-5 84-85 2.24304879179423E-4 86-87 1.9938211482615375E-4 88-89 2.741504078859614E-4 90-91 1.7445935047288454E-4 92-93 9.969105741307687E-5 94-95 1.9938211482615375E-4 96-97 3.2399593659249984E-4 98-99 3.987642296523075E-4 100-101 3.489187009457691E-4 102-103 8.224512236578842E-4 104-105 3.987642296523075E-4 106-107 3.987642296523075E-4 108-109 4.7353252271211516E-4 110-111 6.978374018915381E-4 112-113 2.741504078859614E-4 114-115 3.489187009457691E-4 116-117 6.230691088317305E-4 118-119 9.969105741307687E-4 120-121 4.984552870653843E-4 122-123 5.73223580125192E-4 124-125 2.492276435326922E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2006198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.35625632909716 #Duplication Level Percentage of deduplicated Percentage of total 1 55.34061739205707 19.56637053924062 2 14.335875894317393 10.137258056432213 3 9.718469065933414 10.308260502646297 4 6.7632680789321125 9.56495359284497 5 4.335680517870328 7.664671587544801 6 2.78542046685369 5.908922400623553 7 1.7056278554964543 4.221323096268665 8 1.1538621862415281 3.2636997780166337 9 0.760396849075168 2.4196307316965497 >10 2.841232451315213 17.28048554495494 >50 0.17731260657588868 4.31218699965884 >100 0.08052380808159135 4.95729051947518 >500 0.0015700916459867615 0.3384752345453993 >1k 1.427356041806146E-4 0.05647141605133467 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.984552870653844E-5 0.0 5 0.0 0.0 0.0 4.984552870653844E-5 0.0 6 0.0 0.0 0.0 4.984552870653844E-5 0.0 7 0.0 0.0 0.0 4.984552870653844E-5 0.0 8 0.0 0.0 0.0 4.984552870653844E-5 0.0 9 0.0 0.0 0.0 4.984552870653844E-5 0.0 10-11 0.0 0.0 0.0 4.984552870653844E-5 0.0 12-13 0.0 0.0 0.0 4.984552870653844E-5 0.0 14-15 0.0 0.0 0.0 4.984552870653844E-5 0.0 16-17 0.0 0.0 0.0 4.984552870653844E-5 0.0 18-19 0.0 0.0 0.0 4.984552870653844E-5 0.0 20-21 0.0 0.0 0.0 4.984552870653844E-5 0.0 22-23 0.0 0.0 0.0 1.4953658611961533E-4 0.0 24-25 0.0 0.0 0.0 2.2430487917942296E-4 0.0 26-27 0.0 0.0 0.0 3.987642296523075E-4 0.0 28-29 0.0 0.0 0.0 8.722967523644226E-4 0.0 30-31 0.0 0.0 0.0 0.0022430487917942297 0.0 32-33 0.0 0.0 0.0 0.0068786829615023045 0.0 34-35 0.0 0.0 0.0 0.015103195198081147 0.0 36-37 0.0 0.0 0.0 0.028237492012254024 0.0 38-39 0.0 0.0 0.0 0.049995065292658056 0.0 40-41 0.0 0.0 0.0 0.07369661419261708 0.0 42-43 0.0 0.0 0.0 0.10190918344051783 0.0 44-45 0.0 0.0 0.0 0.14158622429092244 0.0 46-47 0.0 0.0 0.0 0.18846594403942182 0.0 48-49 0.0 0.0 0.0 0.23778809469454162 0.0 50-51 0.0 0.0 0.0 0.28357121281149716 0.0 52-53 0.0 2.492276435326922E-5 0.0 0.33795268463033057 0.0 54-55 0.0 4.984552870653844E-5 0.0 0.4058173719642827 0.0 56-57 0.0 4.984552870653844E-5 0.0 0.4966857707963023 0.0 58-59 0.0 4.984552870653844E-5 0.0 0.599691555868364 0.0 60-61 0.0 4.984552870653844E-5 0.0 0.6928030034921777 0.0 62-63 0.0 4.984552870653844E-5 0.0 0.7903507031708734 0.0 64-65 0.0 4.984552870653844E-5 0.0 0.9043225045583736 2.492276435326922E-5 66-67 0.0 4.984552870653844E-5 0.0 1.0563264443489626 4.984552870653844E-5 68-69 0.0 4.984552870653844E-5 0.0 1.2375897094902895 4.984552870653844E-5 70-71 0.0 4.984552870653844E-5 0.0 1.4114259908543425 4.984552870653844E-5 72-73 0.0 4.984552870653844E-5 0.0 1.5809007884565731 4.984552870653844E-5 74-75 0.0 4.984552870653844E-5 0.0 1.7663261552448961 4.984552870653844E-5 76-77 0.0 4.984552870653844E-5 0.0 1.9941949897268365 4.984552870653844E-5 78-79 0.0 4.984552870653844E-5 0.0 2.2787631131124644 4.984552870653844E-5 80-81 0.0 4.984552870653844E-5 0.0 2.556975931588009 4.984552870653844E-5 82-83 0.0 4.984552870653844E-5 0.0 2.8232507459383367 4.984552870653844E-5 84-85 0.0 4.984552870653844E-5 0.0 3.1250903450207805 4.984552870653844E-5 86-87 0.0 4.984552870653844E-5 0.0 3.4709435459510978 4.984552870653844E-5 88-89 4.984552870653844E-5 4.984552870653844E-5 0.0 3.8646235316753383 4.984552870653844E-5 90-91 4.984552870653844E-5 4.984552870653844E-5 0.0 4.278540802054433 4.984552870653844E-5 92-93 4.984552870653844E-5 4.984552870653844E-5 0.0 4.675984125196017 4.984552870653844E-5 94-95 4.984552870653844E-5 4.984552870653844E-5 0.0 5.117690277829007 4.984552870653844E-5 96-97 4.984552870653844E-5 4.984552870653844E-5 0.0 5.5969799591067275 4.984552870653844E-5 98-99 4.984552870653844E-5 4.984552870653844E-5 0.0 6.133118465874256 4.984552870653844E-5 100-101 4.984552870653844E-5 4.984552870653844E-5 0.0 6.722766147708252 7.476829305980766E-5 102-103 4.984552870653844E-5 4.984552870653844E-5 0.0 7.293397760340705 9.969105741307687E-5 104-105 4.984552870653844E-5 4.984552870653844E-5 0.0 7.8703348323545335 9.969105741307687E-5 106-107 4.984552870653844E-5 4.984552870653844E-5 0.0 8.513092925025347 9.969105741307687E-5 108-109 4.984552870653844E-5 4.984552870653844E-5 0.0 9.176736294224199 9.969105741307687E-5 110-111 4.984552870653844E-5 4.984552870653844E-5 0.0 9.88130284249112 9.969105741307687E-5 112-113 4.984552870653844E-5 4.984552870653844E-5 0.0 10.57968854519843 9.969105741307687E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2980 0.0 58.89372 2 GGTATCA 3295 0.0 58.499584 1 GTACATG 16390 0.0 52.19671 1 TACATGG 16670 0.0 50.641903 2 ACATGGG 17050 0.0 47.803467 3 CATGGGA 11620 0.0 43.313915 4 GTACCTG 4175 0.0 42.6067 1 CATGGGG 5560 0.0 41.944447 4 GTATCAA 4675 0.0 40.849487 2 ACCTGGG 4395 0.0 39.932487 3 TCAACGC 5170 0.0 37.168514 5 ATGGGAT 4855 0.0 36.51659 5 ATCAACG 5285 0.0 36.472305 4 CAACGCA 5440 0.0 35.651833 6 ATGGGAG 4440 0.0 35.106014 5 AACGCAG 5640 0.0 34.071987 7 GAGTACA 9840 0.0 32.82978 1 AGTACAT 9565 0.0 31.969908 2 TATCAAC 6075 0.0 31.533543 3 ATGGGGG 3490 0.0 31.365744 5 >>END_MODULE