FastQCFastQC Report
Thu 26 May 2016
SRR936227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006198
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.2635831558001752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.17600456186278723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.15242762678459454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.073.272281
TACCTGG26650.056.7452052
GTATCAA77200.047.892442
GTACATG152250.045.700041
TACATGG156150.044.1147732
ACATGGG156600.043.5687073
TAACGCA3400.042.0256234
CATGGGA111400.040.7241134
ATGGGAT47050.038.5942045
ACCTGGG40450.036.9434173
TCAACGC105100.036.764095
ATCAACG105400.036.6029624
CAACGCA108100.035.8839766
GTACCTG42900.035.5478821
AACGCAG111250.034.9214177
TATCAAC111150.034.8701133
CATGGGG46350.034.2959944
TATAACG4000.034.234232
ATGGGAG39100.032.2805525
CGTATAG1701.2469172E-831.537311
Produced by FastQC (version 0.11.5)