FastQCFastQC Report
Thu 26 May 2016
SRR936226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708102
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.057.1518331
GTACATG171550.056.251511
TACATGG174800.054.2866822
ACATGGG174400.052.5349433
CATGGGA103950.046.2445874
CATGGGG72200.045.07384
GTATCAA40300.044.5836832
TCAACGC46650.038.2598845
ATCAACG47100.038.0206574
CAACGCA47300.037.7341166
AACGCAG48400.036.8765227
ATGGGAG46300.036.621655
ATGGGGG45750.034.9812435
ATGGGAT45600.034.1830255
GAGTACA101100.033.601531
AGTACAT98450.032.3304752
TATCAAC58600.031.7775753
ACGCAGA59800.029.2496178
CGCAGAG60800.028.279289
ATGGGGA37700.027.1432085
Produced by FastQC (version 0.11.5)