##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2708102 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.253127836396118 31.0 30.0 33.0 25.0 34.0 2 29.18139309376087 31.0 30.0 34.0 23.0 34.0 3 29.19620309722455 31.0 30.0 34.0 23.0 34.0 4 32.61723967561045 35.0 33.0 37.0 25.0 37.0 5 32.77757004721388 35.0 33.0 37.0 26.0 37.0 6 32.812044745729665 35.0 33.0 37.0 26.0 37.0 7 32.619841497846096 35.0 33.0 37.0 25.0 37.0 8 32.58645760019379 35.0 33.0 37.0 25.0 37.0 9 33.90591048638493 38.0 34.0 39.0 24.0 39.0 10-11 33.70620825951164 37.5 33.5 39.0 22.0 39.0 12-13 33.743431746662424 37.5 33.0 39.0 23.0 39.0 14-15 34.79579498851963 39.0 34.0 41.0 23.5 41.0 16-17 34.80320626771075 39.0 34.0 41.0 23.5 41.0 18-19 34.78508822784371 39.0 34.0 41.0 23.0 41.0 20-21 34.82884802714226 39.0 34.0 41.0 24.0 41.0 22-23 34.70513130598478 39.0 34.0 41.0 23.5 41.0 24-25 34.633596703521505 39.0 34.0 41.0 23.5 41.0 26-27 34.67765634381571 39.0 34.0 40.0 24.0 41.0 28-29 34.544915405697424 39.0 33.0 40.0 23.5 41.0 30-31 34.46849417045591 38.0 33.0 40.0 24.0 41.0 32-33 34.27391952001808 38.0 33.0 40.0 22.5 41.0 34-35 34.23650438572845 38.0 33.0 40.0 23.0 41.0 36-37 34.14930770702138 38.0 33.0 40.0 22.0 41.0 38-39 33.894392825676434 38.0 33.0 40.0 19.5 41.0 40-41 33.977149863631425 38.0 33.0 40.0 21.0 41.0 42-43 33.89640364358506 38.0 33.0 40.0 19.5 41.0 44-45 33.760053904912 38.0 33.0 40.0 18.5 41.0 46-47 33.71177119621048 38.0 33.0 40.0 19.0 41.0 48-49 33.61764863361867 38.0 33.0 40.0 18.5 41.0 50-51 33.46863744423216 38.0 32.0 40.0 18.0 41.0 52-53 33.290954328898984 38.0 32.0 40.0 16.0 41.0 54-55 33.101624680311154 38.0 32.0 40.0 14.5 41.0 56-57 32.89399586869328 37.0 31.5 40.0 13.0 41.0 58-59 32.64989409556952 37.0 31.0 40.0 11.0 41.0 60-61 32.39864488117508 37.0 31.0 40.0 8.0 41.0 62-63 32.15263863768794 36.0 31.0 40.0 8.0 41.0 64-65 31.868476150455187 36.0 30.5 39.0 7.0 41.0 66-67 31.57279009431698 35.5 30.0 39.0 6.5 41.0 68-69 31.287831846806363 35.0 30.0 39.0 2.0 40.0 70-71 30.95575628244431 35.0 30.0 38.5 2.0 40.0 72-73 30.636382603018646 35.0 30.0 37.5 2.0 40.0 74-75 30.303632950309847 34.5 29.0 37.0 2.0 39.0 76-77 29.983997094644145 34.0 29.0 36.5 2.0 39.0 78-79 29.65129267656831 34.0 29.0 36.0 2.0 39.0 80-81 29.34144356453339 34.0 29.0 36.0 2.0 37.5 82-83 29.03654589081209 34.0 29.0 35.0 2.0 37.0 84-85 29.075474815941202 34.0 29.0 35.0 2.0 37.0 86-87 28.957472613660784 34.0 29.0 35.0 2.0 36.0 88-89 28.785882695703485 34.0 29.0 35.0 2.0 36.0 90-91 28.611236762869346 34.0 29.0 35.0 2.0 36.0 92-93 28.477575992337066 34.0 29.0 35.0 2.0 35.5 94-95 28.354142126108986 34.0 29.0 35.0 2.0 35.0 96-97 28.241592820359056 34.0 29.0 35.0 2.0 35.0 98-99 28.122215485236524 34.0 29.0 35.0 2.0 35.0 100-101 28.004142384592605 34.0 28.0 35.0 2.0 35.0 102-103 27.05135626353808 32.5 25.5 34.0 2.0 35.0 104-105 27.704346808207372 34.0 27.0 35.0 2.0 35.0 106-107 27.82312390744514 34.0 27.0 35.0 2.0 35.0 108-109 27.760763257809344 34.0 27.0 35.0 2.0 35.0 110-111 27.64178066409611 34.0 27.0 35.0 2.0 35.0 112-113 27.588731517498232 34.0 27.0 35.0 2.0 35.0 114-115 27.464174355323394 34.0 27.0 35.0 2.0 35.0 116-117 27.32312760006824 34.0 27.0 35.0 2.0 35.0 118-119 27.09251054797788 34.0 26.5 35.0 2.0 35.0 120-121 26.86007063249464 34.0 25.0 35.0 2.0 35.0 122-123 26.514699040139554 34.0 25.0 35.0 2.0 35.0 124-125 24.514166009995193 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6795720632380693 1101 2 0.7771947446399032 1101 3 0.706899930305088 1101 4 0.8281852043829687 1101 5 0.7445026720571235 1101 6 0.7353783126213358 1101 7 0.792675337240837 1101 8 0.8189587571714938 1101 9 0.8947447432032476 1101 10-11 1.0722828343901298 1101 12-13 1.0342879409839938 1101 14-15 1.1494955177126016 1101 16-17 1.109337600678188 1101 18-19 1.0792257648283794 1101 20-21 1.0256002525282781 1101 22-23 1.1486697306182805 1101 24-25 1.2250855661189277 1101 26-27 1.1010596818443759 1101 28-29 1.2547605787596439 1101 30-31 1.311654585224126 1101 32-33 1.346409773510949 1101 34-35 1.214924029822221 1101 36-37 1.2187294046028612 1101 38-39 1.3402773389181633 1101 40-41 1.3855431681485655 1101 42-43 1.4044299360257142 1101 44-45 1.387784394821331 1101 46-47 1.1859365816568754 1101 48-49 1.228145741807161 1101 50-51 1.2368580392631685 1101 52-53 1.1796109104325652 1101 54-55 1.1686222807351143 1101 56-57 1.1281651881735968 1101 58-59 1.1926941113648084 1101 60-61 1.184509191545068 1101 62-63 1.2572903459631775 1101 64-65 1.1819981044775112 1101 66-67 1.304078324380356 1101 68-69 1.1333645743284997 1101 70-71 1.1456501495094322 1101 72-73 1.0802019480650955 1101 74-75 1.0757253179175343 1101 76-77 1.0295808433311748 1101 78-79 1.0807447070009104 1101 80-81 1.173935633445815 1101 82-83 1.1086997095271514 1101 84-85 0.9523985056136688 1101 86-87 1.0596795692657217 1101 88-89 1.0205172344447178 1101 90-91 1.040237054665873 1101 92-93 1.0332309939125182 1101 94-95 1.254314070098971 1101 96-97 1.2677494794494422 1101 98-99 1.285175033470047 1101 100-101 1.2710081861631828 1101 102-103 1.2926408855106253 1101 104-105 1.2313859074636326 1101 106-107 1.2223723627671426 1101 108-109 1.1030478732231828 1101 110-111 1.1660777466095844 1101 112-113 1.1240286510585804 1101 114-115 1.1500147255129072 1101 116-117 1.1775424282350464 1101 118-119 1.2868432528360216 1101 120-121 1.2342149727539784 1101 122-123 1.0815426886748227 1101 124-125 0.7813408501096433 1102 1 0.44135424786077593 1102 2 0.4354358396359679 1102 3 0.5364503789618844 1102 4 0.5665314376866419 1102 5 0.4258128786349147 1102 6 0.46548065946694805 1102 7 0.4481608909713586 1102 8 0.5543956252429467 1102 9 0.5683838734584157 1102 10-11 0.7800851094263095 1102 12-13 0.6985001226252265 1102 14-15 0.771637938637106 1102 16-17 0.7658362736395787 1102 18-19 0.6804673409379873 1102 20-21 0.6357492344025601 1102 22-23 0.7297445950669186 1102 24-25 0.8626641847370067 1102 26-27 0.7029627757343206 1102 28-29 0.6712390485815902 1102 30-31 0.659149250756272 1102 32-33 0.7992194146975962 1102 34-35 0.7835989852629126 1102 36-37 0.75826790174591 1102 38-39 0.801138613291549 1102 40-41 0.7878245718557793 1102 42-43 0.8822775234820099 1102 44-45 0.8448735126990599 1102 46-47 0.6571675502109215 1102 48-49 0.7027848559433707 1102 50-51 0.672419434293893 1102 52-53 0.6671708908378946 1102 54-55 0.7624428111854797 1102 56-57 0.7874999171311714 1102 58-59 0.8641717533554854 1102 60-61 0.902843738070203 1102 62-63 0.9056770463921211 1102 64-65 0.9283545527173409 1102 66-67 0.8776507210805207 1102 68-69 0.7674419630487073 1102 70-71 0.7157206224492754 1102 72-73 0.7140219138181827 1102 74-75 0.7025012721892239 1102 76-77 0.8487278387306532 1102 78-79 0.8358789955373069 1102 80-81 0.616909053924001 1102 82-83 0.6048475186671531 1102 84-85 0.7208472939216826 1102 86-87 0.7504906208078523 1102 88-89 0.7296244245569419 1102 90-91 0.7410218984897128 1102 92-93 0.8373155966671959 1102 94-95 0.811549048537465 1102 96-97 0.925716018413393 1102 98-99 0.7919729372795743 1102 100-101 0.8664389578524414 1102 102-103 0.813043003349609 1102 104-105 0.9293843865171496 1102 106-107 0.881793397538086 1102 108-109 0.8573910251371082 1102 110-111 0.8014845757745661 1102 112-113 0.8122059608812933 1102 114-115 0.8683055587039981 1102 116-117 0.8993576823418898 1102 118-119 0.8557060581902043 1102 120-121 0.8513974781119309 1102 122-123 0.7186146275858931 1102 124-125 0.7134917777877234 1103 1 0.4456643880959632 1103 2 0.48939563030375055 1103 3 0.5568156763917749 1103 4 0.5645324784975969 1103 5 0.5692496784058818 1103 6 0.571433960977366 1103 7 0.5074714721100975 1103 8 0.46043565028497824 1103 9 0.661927266885705 1103 10-11 0.7037091233800723 1103 12-13 0.571576526870885 1103 14-15 0.6999525663382542 1103 16-17 0.7429981662440426 1103 18-19 0.7951194873239729 1103 20-21 0.5981477689955952 1103 22-23 0.6832108148370253 1103 24-25 0.7704590112615435 1103 26-27 0.6017814113719453 1103 28-29 0.7082894381249645 1103 30-31 0.8121302730460656 1103 32-33 0.8990217634759858 1103 34-35 0.7892040854723064 1103 36-37 0.6999760863836713 1103 38-39 0.8268522636332776 1103 40-41 0.9210149177930944 1103 42-43 0.8838455972220842 1103 44-45 0.9419097408275903 1103 46-47 0.9024414713177933 1103 48-49 0.9689656679709202 1103 50-51 0.9186122616128145 1103 52-53 0.811861131719084 1103 54-55 0.8108062875712108 1103 56-57 0.7310570573203989 1103 58-59 0.8572683448764025 1103 60-61 0.8859811595098179 1103 62-63 0.9150494076004492 1103 64-65 0.7485501746040306 1103 66-67 0.7998269588542826 1103 68-69 0.8603083847022965 1103 70-71 0.8342097279529419 1103 72-73 0.7917327179480367 1103 74-75 0.7196221784426307 1103 76-77 0.7831680256908982 1103 78-79 0.7350043350410758 1103 80-81 0.716522907059943 1103 82-83 0.7237025259010785 1103 84-85 0.7207312553934635 1103 86-87 0.7843463882387844 1103 88-89 0.7903063871659626 1103 90-91 0.7778244070219706 1103 92-93 0.7544667661544864 1103 94-95 0.7699697545252988 1103 96-97 0.8701043930457644 1103 98-99 0.7477809549097145 1103 100-101 0.7530022695127485 1103 102-103 0.7595805818858885 1103 104-105 0.7120503840247352 1103 106-107 0.7213426226782254 1103 108-109 0.6434572300997203 1103 110-111 0.5766404000890191 1103 112-113 0.7116550939513431 1103 114-115 0.6909833122109497 1103 116-117 0.6027482306341092 1103 118-119 0.5735994135326408 1103 120-121 0.59854017667951 1103 122-123 0.6803468245916697 1103 124-125 0.7143746295540225 1104 1 0.509389755745822 1104 2 0.587750624980373 1104 3 0.6213486972572859 1104 4 0.6327033990548827 1104 5 0.48783705403774746 1104 6 0.4845072458904909 1104 7 0.4565204906250173 1104 8 0.4152874868256262 1104 9 0.5837570040387092 1104 10-11 0.652277439361697 1104 12-13 0.6869478172051373 1104 14-15 0.7193251654408712 1104 16-17 0.7429353994381813 1104 18-19 0.7923273032826756 1104 20-21 0.7266304549599596 1104 22-23 0.7407044847778366 1104 24-25 0.7157639361597248 1104 26-27 0.6892781241616674 1104 28-29 0.7855113589115419 1104 30-31 0.8024745119599146 1104 32-33 0.9097397588694349 1104 34-35 0.8713198590066611 1104 36-37 0.8964421167201024 1104 38-39 0.8938299442935786 1104 40-41 0.7372043922181959 1104 42-43 0.930021419598674 1104 44-45 0.9335388484456288 1104 46-47 0.8864946522912334 1104 48-49 0.8223927302065164 1104 50-51 0.8602493250576089 1104 52-53 0.8756138592586851 1104 54-55 0.7610398820180322 1104 56-57 0.8272404009843228 1104 58-59 0.893072810933127 1104 60-61 0.8907316678941228 1104 62-63 0.8442223513947624 1104 64-65 0.9572900928870105 1104 66-67 0.9824240196725285 1104 68-69 0.844919170957926 1104 70-71 0.806974674419294 1104 72-73 0.792947470228917 1104 74-75 0.8409175554903427 1104 76-77 0.7412103204324474 1104 78-79 0.7157864802559963 1104 80-81 0.7326538956972151 1104 82-83 0.723857243395198 1104 84-85 0.808357900836004 1104 86-87 0.7916484745752221 1104 88-89 0.7790306523732085 1104 90-91 0.767612542765221 1104 92-93 0.726199332781448 1104 94-95 0.8190747481558063 1104 96-97 0.7984923225920078 1104 98-99 0.8291434194799976 1104 100-101 0.8075103317211969 1104 102-103 0.8148789425564011 1104 104-105 0.7329551637760119 1104 106-107 0.7552045902105142 1104 108-109 0.8060809812951071 1104 110-111 0.7977606416543139 1104 112-113 0.7562020372454477 1104 114-115 0.8277742846919445 1104 116-117 0.8092392464510247 1104 118-119 0.7577045407437915 1104 120-121 0.7929536788172875 1104 122-123 0.8320732389987242 1104 124-125 0.8248527645494335 1105 1 0.2736547307130017 1105 2 0.34969467334431314 1105 3 0.40815858105752056 1105 4 0.4538081396600404 1105 5 0.2776321689138044 1105 6 0.20374008626902196 1105 7 0.26698597249673384 1105 8 0.20625018311615406 1105 9 0.3675226027898475 1105 10-11 0.46062691719011895 1105 12-13 0.3788138072890206 1105 14-15 0.4513549491013862 1105 16-17 0.40798250209998344 1105 18-19 0.2938487076325629 1105 20-21 0.26832200646127546 1105 22-23 0.2526887504793791 1105 24-25 0.32665501263509356 1105 26-27 0.213310744943783 1105 28-29 0.4294153464309076 1105 30-31 0.3864512655742445 1105 32-33 0.581260653351535 1105 34-35 0.49232819927312477 1105 36-37 0.43313461756676475 1105 38-39 0.4886502728435289 1105 40-41 0.3384073743805729 1105 42-43 0.4721868792355153 1105 44-45 0.5132458416118695 1105 46-47 0.43364634997960394 1105 48-49 0.3684156006503727 1105 50-51 0.45367002171417425 1105 52-53 0.4285279065696699 1105 54-55 0.47452205175169837 1105 56-57 0.44522404419370787 1105 58-59 0.5685578879149986 1105 60-61 0.5044102237249817 1105 62-63 0.5626962691265689 1105 64-65 0.5116001075814403 1105 66-67 0.5430957290915472 1105 68-69 0.4830620742969636 1105 70-71 0.3515897930484435 1105 72-73 0.5538342415641786 1105 74-75 0.5376128424190973 1105 76-77 0.403842721945054 1105 78-79 0.4440898791127026 1105 80-81 0.4553662014538027 1105 82-83 0.4276863293857467 1105 84-85 0.40958099825803274 1105 86-87 0.5102646754738842 1105 88-89 0.4452946623351899 1105 90-91 0.4089573841712948 1105 92-93 0.3895809024898398 1105 94-95 0.47306407341196177 1105 96-97 0.4063576474750725 1105 98-99 0.4617552591372842 1105 100-101 0.5131042351577264 1105 102-103 0.5710324061167498 1105 104-105 0.5747234432611741 1105 106-107 0.6025979451938035 1105 108-109 0.5895333303540724 1105 110-111 0.7113298790898313 1105 112-113 0.6445739197295168 1105 114-115 0.6805444512151837 1105 116-117 0.6670728166417597 1105 118-119 0.6702871022847532 1105 120-121 0.7905362358764734 1105 122-123 0.867516985581041 1105 124-125 0.9303079227848201 1106 1 -0.2120191454386955 1106 2 -0.3629068310127117 1106 3 -0.1515064770910186 1106 4 -0.0677107338230627 1106 5 -0.07530484805974424 1106 6 -0.057503205986314754 1106 7 -0.24523371117152237 1106 8 -0.14104355282709946 1106 9 -0.13738716585952204 1106 10-11 -0.13597797666580647 1106 12-13 -0.08289721108216241 1106 14-15 -0.2715054231082519 1106 16-17 -0.21556490741672008 1106 18-19 -0.28913490438121414 1106 20-21 -0.2677470425823003 1106 22-23 -0.2725075940240558 1106 24-25 -4.270981221983384 1106 26-27 -1.3538358276833478 1106 28-29 -0.8568564695442902 1106 30-31 -0.8478753804184009 1106 32-33 -5.562261683724241 1106 34-35 -1.308415232793223 1106 36-37 -0.5341908793383965 1106 38-39 -0.17802181961449293 1106 40-41 -0.7979406043791428 1106 42-43 -0.31111880810539816 1106 44-45 -0.32604301999774066 1106 46-47 -0.30918842130294166 1106 48-49 -0.06064517214121423 1106 50-51 -0.040164658615907456 1106 52-53 -0.04146964283995658 1106 54-55 -0.2546341497877691 1106 56-57 -0.17545857391198183 1106 58-59 0.03729024143144244 1106 60-61 0.09475958532161854 1106 62-63 0.06546965885430467 1106 64-65 0.08538771670523815 1106 66-67 -0.030852909768537273 1106 68-69 0.09990088036002831 1106 70-71 0.08920113913918826 1106 72-73 -0.07860568765883613 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-1.9838985771779747 2216 74-75 -1.787382204691312 2216 76-77 -1.6550801655158125 2216 78-79 -1.457478290035855 2216 80-81 -1.6281099116554394 2216 82-83 -1.6386265393805957 2216 84-85 -1.9358020721857585 2216 86-87 -1.6988467511058474 2216 88-89 -1.520718339263432 2216 90-91 -1.5164004950329968 2216 92-93 -1.4680889236637356 2216 94-95 -1.4828761865725895 2216 96-97 -1.7305073881277728 2216 98-99 -1.5431125026624493 2216 100-101 -1.6807469236603723 2216 102-103 -1.5244037168877362 2216 104-105 -1.504764638716523 2216 106-107 -1.6754088671369942 2216 108-109 -1.7710688435429347 2216 110-111 -1.6931756433820624 2216 112-113 -1.7190600548800745 2216 114-115 -1.816145378552946 2216 116-117 -1.772772923240911 2216 118-119 -1.7722642852619934 2216 120-121 -1.8277754202222702 2216 122-123 -1.7396722390388248 2216 124-125 -1.7374906416148619 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107695.0 3 16961.0 4 10600.0 5 9163.0 6 9579.0 7 11059.0 8 12069.0 9 13511.0 10 14839.0 11 15900.0 12 16999.0 13 17112.0 14 17057.0 15 16359.0 16 15432.0 17 14077.0 18 12669.0 19 12146.0 20 12275.0 21 13121.0 22 15131.0 23 20379.0 24 27890.0 25 37808.0 26 44275.0 27 47285.0 28 55260.0 29 65341.0 30 77386.0 31 94137.0 32 116666.0 33 150160.0 34 199404.0 35 278452.0 36 426413.0 37 522533.0 38 160551.0 39 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.21487469027986 17.506545203445974 9.085185499743362 23.193394606530802 2 19.466252501295187 19.893415930079314 37.17200676342095 23.468324805204542 3 17.25327083443343 27.2560735364006 30.276197231934848 25.21445839723112 4 11.18256778762892 15.554025161645294 37.163498997448386 36.0999080532774 5 11.079469083539877 42.18410736494451 35.20692950556706 11.529494045948557 6 32.26532234747258 37.03769487464768 18.145170373034546 12.551812404845192 7 28.3738239348438 34.13724942089903 21.501604274915373 15.987322369341788 8 27.517866994379013 33.78359316205735 19.574227511441745 19.12431233212189 9 27.44354794039992 12.92025627296394 18.533852772290025 41.10234301434612 10-11 24.594838511463575 26.06125944219765 29.478378585967203 19.865523460371573 12-13 25.13462199951958 25.10419415035678 28.593371123195677 21.167812726927963 14-15 22.646277921588755 26.898501862977888 25.186574742525007 25.268645472908354 16-17 23.116748980303626 29.225695443912958 25.698564254950035 21.95899132083338 18-19 22.28991162978081 28.197452104641034 27.19242595841493 22.320210307163226 20-21 23.224932944834652 27.57765188477313 26.9528437907757 22.244571379616524 22-23 23.09965744077192 27.643755404173504 26.826266459225252 22.430320695829323 24-25 22.812356734774212 27.583897685618652 26.91459578504128 22.68914979456586 26-27 22.70913156891881 27.803727101655205 26.730355649957016 22.756785679468962 28-29 22.595410516971366 27.868223795549657 26.837504768171765 22.698860919307208 30-31 22.759620511744906 27.393736050941115 27.135507675582744 22.711135761731242 32-33 22.554646251514924 27.66373934936305 27.00754859393498 22.774065805187046 34-35 23.009317512732768 27.68053661783427 26.830042401006622 22.480103468426343 36-37 22.923470758368698 27.44244313088689 26.72632757680019 22.907758533944218 38-39 22.50379006011845 27.625536336664798 26.988809929431305 22.88186367378545 40-41 22.772584341109916 27.52523895747721 27.0188811902814 22.683295511131472 42-43 22.590866129985503 27.449264022058568 27.09735203771994 22.862517810235985 44-45 22.446788102720596 27.5895385800449 27.20561124005724 22.75806207717726 46-47 22.758139367424807 27.671956743902225 26.646664629536115 22.923239259136853 48-49 22.662809336909284 27.527416914719634 26.897619023880658 22.912154724490424 50-51 22.72236650329036 27.480139863806713 27.028100897209555 22.769392735693373 52-53 22.852580293523346 27.478246452077943 26.890224898672997 22.778948355725714 54-55 22.46859147453207 27.519748897966718 27.206296014216807 22.805363613284406 56-57 22.73705076079244 27.666401594201318 26.979785747348338 22.616761897657902 58-59 22.96619171120778 27.286579764951423 26.7626289267464 22.984599597094395 60-61 22.75167453142846 27.359382379560948 27.349135233160432 22.53980785585016 62-63 22.51064043490816 27.46718506860616 27.197730779087365 22.82444371739832 64-65 22.35070841886463 27.601065407025587 27.15981068457165 22.888415489538133 66-67 22.475373312208962 27.77113820320126 26.896406819791896 22.85708166479788 68-69 22.55170563656475 27.454359513672806 27.156087056784763 22.837847792977684 70-71 22.532927806694293 27.28431379608494 27.180845790020875 23.00191260719989 72-73 22.520993074284124 27.558278502364487 26.900066855101674 23.020661568249718 74-75 22.253648652915146 27.809430895629887 27.08751948099185 22.849400970463115 76-77 22.351632895306043 27.45227860906318 27.101533055511297 23.09455544011948 78-79 22.561047998878916 27.43992131736909 27.054853514159127 22.944177169592862 80-81 22.52662950881176 27.487513321216532 26.934890546312047 23.05096662365966 82-83 22.597943352082055 27.66772300538829 26.98554630264215 22.748787339887507 84-85 22.31726462511671 27.381960603591573 27.175523556366954 23.125251214924766 86-87 22.234799760200037 27.494446732854755 27.17130391321856 23.09944959372665 88-89 22.349199878290513 27.45673331147289 27.15637379582938 23.03769301440721 90-91 22.426820777487638 27.4702009106403 27.149458779965023 22.95351953190703 92-93 22.412715234722626 27.193649398653662 27.367960444446087 23.025674922177625 94-95 22.144280918078117 27.250385142011424 27.45455102706998 23.15078291284048 96-97 22.3197428149518 27.21358123754426 27.353587714436266 23.11308823306767 98-99 22.20629052558659 27.325491762916904 27.21482329694912 23.253394414547383 100-101 22.038244666905456 27.281958871381672 27.293959949632395 23.38583651208047 102-103 21.98845637797026 26.98178097324096 27.507006359313095 23.522756289475684 104-105 21.839574297516496 27.129634057380326 27.459405407171882 23.571386237931293 106-107 21.960200296482515 27.11469043351844 27.503193676861382 23.42191559313766 108-109 22.03510749116626 27.122894682326944 27.249017109150643 23.592980717356152 110-111 21.989453395963295 27.449807067786697 27.193314957430104 23.367424578819904 112-113 21.982099913684472 27.146094799374097 27.544734060476262 23.327071226465172 114-115 21.775712443878813 27.242442599314902 27.316627712404735 23.665217244401543 116-117 21.753326301279266 27.16735556301883 27.562968609996286 23.516349525705614 118-119 21.711449848074263 27.250364789786367 27.289156303953394 23.74902905818598 120-121 21.739848896635255 27.31274556143098 27.394316268352487 23.553089273581282 122-123 21.75965596295974 27.202810256194375 27.26222503356159 23.775308747284285 124-125 21.858667574071102 27.002938048045294 27.074409071631838 24.063985306251766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 38.0 2 57.0 3 73.0 4 80.0 5 86.0 6 90.0 7 107.5 8 123.5 9 136.5 10 144.0 11 169.0 12 209.5 13 254.5 14 392.5 15 483.0 16 496.0 17 612.5 18 813.5 19 1161.5 20 1673.0 21 2484.0 22 3833.0 23 5936.5 24 8134.0 25 10810.5 26 15002.0 27 19385.0 28 22846.5 29 27497.5 30 33775.0 31 39655.5 32 45877.0 33 50771.5 34 56047.0 35 61284.5 36 67221.5 37 73744.5 38 77186.5 39 79265.0 40 81024.5 41 85104.0 42 88603.0 43 91952.0 44 96807.5 45 101814.5 46 105284.5 47 105804.0 48 103817.5 49 100772.5 50 99249.0 51 95524.5 52 91467.0 53 89136.0 54 86734.5 55 81828.5 56 74115.0 57 68190.0 58 63554.0 59 56776.5 60 47389.5 61 38709.5 62 32133.0 63 25671.0 64 20277.5 65 16702.5 66 12981.0 67 10046.5 68 7668.5 69 5311.0 70 3866.5 71 2872.5 72 2188.0 73 1780.5 74 1403.0 75 1097.5 76 757.0 77 482.0 78 339.5 79 260.0 80 189.0 81 120.5 82 77.5 83 54.0 84 34.0 85 29.5 86 28.0 87 20.0 88 15.0 89 12.0 90 9.0 91 5.5 92 5.0 93 3.0 94 3.5 95 4.0 96 1.0 97 3.0 98 5.5 99 4.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.431147718955933E-4 2 1.8463115495649722E-4 3 3.692623099129944E-5 4 4.431147718955933E-4 5 0.0011077869297389832 6 5.538934648694916E-4 7 9.231557747824862E-4 8 0.00132934431568678 9 0.0019201640115475709 10-11 0.0016986066255997745 12-13 0.0020863320510084186 14-15 0.00265868863137356 16-17 0.0024186681299301132 18-19 0.002326352552451865 20-21 0.0026032992848866107 22-23 0.09004461427228369 24-25 0.0020863320510084186 26-27 0.0016986066255997745 28-29 0.001513975470643277 30-31 0.001255491853704181 32-33 0.001772459087582373 34-35 0.0010893238142433335 36-37 7.200615043303391E-4 38-39 0.0011631762762259325 40-41 0.0013478074311824297 42-43 0.0014216598931650285 44-45 0.001384733662173729 46-47 0.0016432172791128253 48-49 4.0618854090429383E-4 50-51 0.0014401230086606783 52-53 0.0014031967776693788 54-55 0.001458586124156328 56-57 0.001384733662173729 58-59 8.677664282955369E-4 60-61 0.0011447131607302827 62-63 0.00132934431568678 64-65 0.001070860698747684 66-67 4.985041183825425E-4 68-69 2.769467324347458E-4 70-71 6.831352733390396E-4 72-73 2.4002050144344636E-4 74-75 4.985041183825425E-4 76-77 4.985041183825425E-4 78-79 2.769467324347458E-4 80-81 5.90819695860791E-4 82-83 2.2155738594779664E-4 84-85 4.615778873912431E-4 86-87 4.2465165639994363E-4 88-89 5.169672338781921E-4 90-91 2.769467324347458E-4 92-93 4.431147718955933E-4 94-95 3.6926230991299444E-4 96-97 4.800410028868927E-4 98-99 4.985041183825425E-4 100-101 4.431147718955933E-4 102-103 9.785451212694352E-4 104-105 4.985041183825425E-4 106-107 6.092828113564407E-4 108-109 7.015983888346894E-4 110-111 9.785451212694352E-4 112-113 5.35430349373842E-4 114-115 4.2465165639994363E-4 116-117 9.785451212694352E-4 118-119 0.00131088120019113 120-121 8.123770818085877E-4 122-123 7.93913966312938E-4 124-125 4.2465165639994363E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2708102.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.52121384391105 #Duplication Level Percentage of deduplicated Percentage of total 1 52.72125160252741 17.672803490869594 2 14.91865759995093 10.001830233440877 3 10.389894112255725 10.448455870575495 4 7.062781598566777 9.470120491935873 5 4.612231893349082 7.730380579733066 6 2.915004822031244 5.8628699997204725 7 1.83455334943433 4.304751859411601 8 1.2258205386602423 3.287279392855057 9 0.85533514787469 2.580468516010869 >10 3.201684200321986 17.908740524491783 >50 0.16772128577826953 3.8618070340088893 >100 0.08972486588033769 5.542652374777382 >500 0.004671610447952807 1.0321675943440973 >1k 6.673729211316628E-4 0.2956720378248635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.6926230991299444E-5 0.0 9 0.0 0.0 0.0 7.385246198259889E-5 0.0 10-11 0.0 0.0 0.0 7.385246198259889E-5 0.0 12-13 0.0 0.0 0.0 7.385246198259889E-5 0.0 14-15 0.0 0.0 0.0 7.385246198259889E-5 0.0 16-17 0.0 0.0 0.0 7.385246198259889E-5 0.0 18-19 0.0 0.0 0.0 7.385246198259889E-5 0.0 20-21 0.0 0.0 0.0 7.385246198259889E-5 0.0 22-23 0.0 0.0 0.0 7.385246198259889E-5 0.0 24-25 0.0 0.0 0.0 1.1077869297389832E-4 0.0 26-27 0.0 0.0 0.0 2.4002050144344636E-4 0.0 28-29 0.0 0.0 0.0 4.985041183825425E-4 5.538934648694916E-5 30-31 0.0 0.0 0.0 0.0016801435101041246 1.1077869297389832E-4 32-33 0.0 0.0 0.0 0.005889733843112262 1.1077869297389832E-4 34-35 0.0 0.0 0.0 0.012204119342624466 1.1077869297389832E-4 36-37 0.0 0.0 0.0 0.0230235050230752 1.1077869297389832E-4 38-39 0.0 0.0 0.0 0.04181895659764662 1.2924180846954805E-4 40-41 0.0 0.0 0.0 0.06127908033006142 1.4770492396519778E-4 42-43 0.0 0.0 0.0 0.08539190916737996 1.4770492396519778E-4 44-45 0.0 0.0 0.0 0.11801623424819302 1.4770492396519778E-4 46-47 0.0 0.0 0.0 0.15667799809608352 1.4770492396519778E-4 48-49 0.0 0.0 0.0 0.19921701619806048 1.4770492396519778E-4 50-51 0.0 0.0 0.0 0.2385803784347857 1.4770492396519778E-4 52-53 0.0 0.0 0.0 0.28593826968112723 1.4770492396519778E-4 54-55 0.0 0.0 0.0 0.34677423523929307 1.4770492396519778E-4 56-57 0.0 0.0 0.0 0.4219560415375787 1.4770492396519778E-4 58-59 0.0 0.0 0.0 0.5098035450658801 1.4770492396519778E-4 60-61 0.0 0.0 0.0 0.5931091221822516 1.4770492396519778E-4 62-63 0.0 0.0 0.0 0.68016271174424 1.4770492396519778E-4 64-65 0.0 0.0 0.0 0.7832422855564525 1.4770492396519778E-4 66-67 7.385246198259889E-5 0.0 0.0 0.9082745036929923 1.4770492396519778E-4 68-69 1.1077869297389832E-4 1.8463115495649722E-5 0.0 1.0609829319575113 1.4770492396519778E-4 70-71 1.1077869297389832E-4 3.6926230991299444E-5 0.0 1.2145037373038385 1.4770492396519778E-4 72-73 1.1077869297389832E-4 3.6926230991299444E-5 0.0 1.370923251782983 1.4770492396519778E-4 74-75 1.1077869297389832E-4 3.6926230991299444E-5 0.0 1.5458428079887685 1.4770492396519778E-4 76-77 1.1077869297389832E-4 3.6926230991299444E-5 0.0 1.754069824548706 1.4770492396519778E-4 78-79 1.1077869297389832E-4 3.6926230991299444E-5 0.0 2.0003123959141864 1.4770492396519778E-4 80-81 1.1077869297389832E-4 3.6926230991299444E-5 0.0 2.2476996804403973 2.0309427045214694E-4 82-83 1.4770492396519778E-4 1.1077869297389833E-4 0.0 2.494219198538312 2.584836169390961E-4 84-85 1.4770492396519778E-4 1.8463115495649722E-4 0.0 2.7612881641828855 2.584836169390961E-4 86-87 1.4770492396519778E-4 1.8463115495649722E-4 0.0 3.0696775823067224 2.584836169390961E-4 88-89 1.8463115495649722E-4 1.8463115495649722E-4 0.0 3.4185566127125195 2.584836169390961E-4 90-91 1.8463115495649722E-4 1.8463115495649722E-4 0.0 3.7919546604965397 2.769467324347458E-4 92-93 1.8463115495649722E-4 1.8463115495649722E-4 0.0 4.159610679361412 2.9540984793039555E-4 94-95 1.8463115495649722E-4 1.8463115495649722E-4 0.0 4.556143010861481 2.9540984793039555E-4 96-97 1.8463115495649722E-4 1.8463115495649722E-4 0.0 4.997485323669492 2.9540984793039555E-4 98-99 1.8463115495649722E-4 1.8463115495649722E-4 0.0 5.481606675080924 2.9540984793039555E-4 100-101 1.8463115495649722E-4 1.8463115495649722E-4 0.0 5.992388765268073 2.9540984793039555E-4 102-103 1.8463115495649722E-4 1.8463115495649722E-4 0.0 6.4924437853522505 2.9540984793039555E-4 104-105 1.8463115495649722E-4 1.8463115495649722E-4 0.0 7.014229892374807 2.9540984793039555E-4 106-107 1.8463115495649722E-4 1.8463115495649722E-4 0.0 7.5828938496408185 2.9540984793039555E-4 108-109 1.8463115495649722E-4 1.8463115495649722E-4 0.0 8.183332828674843 2.9540984793039555E-4 110-111 1.8463115495649722E-4 1.8463115495649722E-4 0.0 8.81992258784935 2.9540984793039555E-4 112-113 1.8463115495649722E-4 1.8463115495649722E-4 0.0 9.449034785248118 2.9540984793039555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3175 0.0 57.151833 1 GTACATG 17155 0.0 56.25151 1 TACATGG 17480 0.0 54.286682 2 ACATGGG 17440 0.0 52.534943 3 CATGGGA 10395 0.0 46.244587 4 CATGGGG 7220 0.0 45.0738 4 GTATCAA 4030 0.0 44.583683 2 TCAACGC 4665 0.0 38.259884 5 ATCAACG 4710 0.0 38.020657 4 CAACGCA 4730 0.0 37.734116 6 AACGCAG 4840 0.0 36.876522 7 ATGGGAG 4630 0.0 36.62165 5 ATGGGGG 4575 0.0 34.981243 5 ATGGGAT 4560 0.0 34.183025 5 GAGTACA 10110 0.0 33.60153 1 AGTACAT 9845 0.0 32.330475 2 TATCAAC 5860 0.0 31.777575 3 ACGCAGA 5980 0.0 29.249617 8 CGCAGAG 6080 0.0 28.27928 9 ATGGGGA 3770 0.0 27.143208 5 >>END_MODULE