FastQCFastQC Report
Thu 26 May 2016
SRR936226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708102
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40670.15017898144161484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.072.662921
GTATCAA70200.051.4722142
GTACATG149400.047.4936561
TACATGG153700.045.8950422
ACATGGG152500.045.4746443
TCAACGC92300.040.501515
ATCAACG93550.040.0875974
CAACGCA94400.039.8299456
CATGGGA93450.039.5571984
AACGCAG97500.038.5025377
CATGGGG64000.036.6462484
TATCAAC103100.036.3743443
ATGGGAG41500.031.6998675
ACGCAGA116600.031.685298
AGTACAT98000.031.1610852
GAGTACA101900.030.9867551
CGCAGAG118800.030.6978999
ATGGGGG37750.029.9605585
ATGGGAT41250.029.7273715
CAGAGTA136450.024.1546549
Produced by FastQC (version 0.11.5)