FastQCFastQC Report
Thu 26 May 2016
SRR936225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727154
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.061.5533331
GTACATG143700.052.3732221
TACATGG143950.051.042372
ACATGGG147350.048.8148233
CATGGGG42350.045.094874
CATGGGA101600.044.035174
GTATCAA38050.043.9366762
ATGGGAT40450.038.9765055
TCAACGC45250.037.3400845
CAACGCA45300.037.03626
ATCAACG45300.036.64224
ATGGGAG39600.036.0571675
AACGCAG46600.035.875337
AGTACAT84750.031.9409082
GAGTACA88150.031.856291
TATCAAC53300.031.8121623
ATGGGGG25600.031.6063615
ATGGGGA23000.030.5231765
ACGCAGA57000.029.2252838
CGCAGAG57300.028.8646139
Produced by FastQC (version 0.11.5)