##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1727154 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.056721056721056 31.0 30.0 33.0 23.0 34.0 2 28.98957996797043 31.0 30.0 34.0 22.0 34.0 3 29.24066817434925 31.0 30.0 34.0 23.0 34.0 4 32.69617590556488 35.0 33.0 37.0 25.0 37.0 5 32.897047397047395 35.0 33.0 37.0 26.0 37.0 6 32.79428006998797 35.0 33.0 37.0 26.0 37.0 7 32.79551910252357 35.0 33.0 37.0 25.0 37.0 8 32.6993794415553 35.0 33.0 37.0 25.0 37.0 9 34.022032777621455 38.0 34.0 39.0 23.0 39.0 10-11 33.85126746080546 38.0 33.5 39.0 22.5 39.0 12-13 33.83155584273319 38.0 34.0 39.0 22.5 39.0 14-15 34.841708093198406 39.0 34.0 41.0 23.0 41.0 16-17 34.82753477686414 39.0 34.0 41.0 23.5 41.0 18-19 34.83164240131453 39.0 34.0 41.0 23.5 41.0 20-21 34.9361461108853 39.0 34.0 41.0 24.0 41.0 22-23 34.802244327952224 39.0 34.0 41.0 23.5 41.0 24-25 34.746279138976604 39.0 34.0 41.0 24.0 41.0 26-27 34.829628394457 39.0 34.0 41.0 25.0 41.0 28-29 34.7168023233597 39.0 34.0 40.0 24.0 41.0 30-31 34.62999275108068 39.0 33.0 40.0 24.0 41.0 32-33 34.466610678607694 38.0 33.0 40.0 23.5 41.0 34-35 34.420740999355004 38.0 33.0 40.0 24.0 41.0 36-37 34.30773660021052 38.0 33.0 40.0 23.5 41.0 38-39 34.05002420166355 38.0 33.0 40.0 22.0 41.0 40-41 34.14570154137964 38.0 33.0 40.0 22.5 41.0 42-43 34.039525137885796 38.0 33.0 40.0 21.5 41.0 44-45 33.88221229838219 38.0 33.0 40.0 21.5 41.0 46-47 33.85684484417718 38.0 33.0 40.0 21.0 41.0 48-49 33.761645747860356 38.0 33.0 40.0 20.5 41.0 50-51 33.62587094144471 38.0 32.0 40.0 19.5 41.0 52-53 33.46466528172937 38.0 32.0 40.0 18.5 41.0 54-55 33.28341942872494 38.0 32.0 40.0 18.0 41.0 56-57 33.09343029052418 38.0 31.5 40.0 16.0 41.0 58-59 32.86237011870395 37.0 31.0 40.0 13.5 41.0 60-61 32.62939755227386 37.0 31.0 40.0 13.0 41.0 62-63 32.38839443384898 36.5 31.0 40.0 10.5 41.0 64-65 32.11563068493024 36.0 31.0 39.5 8.0 41.0 66-67 31.83166034991668 36.0 30.5 39.0 7.5 41.0 68-69 31.54210684165975 35.0 30.0 39.0 7.0 40.5 70-71 31.215233847126544 35.0 30.0 38.5 3.5 40.0 72-73 30.889690496620453 35.0 30.0 38.0 2.0 40.0 74-75 30.54887056973495 35.0 30.0 37.0 2.0 39.5 76-77 30.219543248604353 34.0 29.0 37.0 2.0 39.0 78-79 29.87708218259634 34.0 29.0 36.0 2.0 39.0 80-81 29.551922411087837 34.0 29.0 36.0 2.0 38.5 82-83 29.230579901965893 34.0 29.0 35.5 2.0 37.0 84-85 29.24277800358277 34.0 29.0 35.0 2.0 37.0 86-87 29.105155938613464 34.0 29.0 35.0 2.0 36.5 88-89 28.917919884387842 34.0 29.0 35.0 2.0 36.0 90-91 28.732430923936143 34.0 29.0 35.0 2.0 36.0 92-93 28.59131901382274 34.0 29.0 35.0 2.0 36.0 94-95 28.453026192221422 34.0 29.0 35.0 2.0 35.0 96-97 28.325819527384354 34.0 29.0 35.0 2.0 35.0 98-99 28.21348154246813 34.0 29.0 35.0 2.0 35.0 100-101 28.09603718023986 34.0 28.5 35.0 2.0 35.0 102-103 27.117093206511985 32.5 26.0 34.0 2.0 35.0 104-105 27.78145637273804 34.0 27.0 35.0 2.0 35.0 106-107 27.90475313724196 34.0 28.0 35.0 2.0 35.0 108-109 27.855457880420623 34.0 28.0 35.0 2.0 35.0 110-111 27.72962717858396 34.0 27.0 35.0 2.0 35.0 112-113 27.68016372599085 34.0 27.0 35.0 2.0 35.0 114-115 27.560163714411104 34.0 27.0 35.0 2.0 35.0 116-117 27.412591465497577 34.0 27.0 35.0 2.0 35.0 118-119 27.18509177525571 34.0 27.0 35.0 2.0 35.0 120-121 26.951700311610892 34.0 26.0 35.0 2.0 35.0 122-123 26.60697308983449 34.0 25.0 35.0 2.0 35.0 124-125 24.583815340149172 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7986500375545447 1101 2 0.8109710288578036 1101 3 0.702339215481576 1101 4 0.6817397986730143 1101 5 0.7066606622079448 1101 6 0.6789541263850509 1101 7 0.6637096223571035 1101 8 0.6597878749182229 1101 9 0.9690765774016228 1101 10-11 0.9881117259082259 1101 12-13 0.8708363472182796 1101 14-15 1.033098265321918 1101 16-17 1.0418264615390314 1101 18-19 0.8617771217390668 1101 20-21 0.8922274408380275 1101 22-23 0.9913683523603467 1101 24-25 1.1211307686095893 1101 26-27 1.0686301929040098 1101 28-29 1.1583525799864773 1101 30-31 1.0575815036270697 1101 32-33 1.2338630999114741 1101 34-35 1.1259567878293097 1101 36-37 0.9314306795053611 1101 38-39 1.185318179347341 1101 40-41 1.0773991809104189 1101 42-43 1.1480456213401524 1101 44-45 1.1815260032373942 1101 46-47 1.0450528169527544 1101 48-49 1.0370142634090982 1101 50-51 1.1637969947900544 1101 52-53 1.086601738076915 1101 54-55 0.9927707943601476 1101 56-57 1.055247401423074 1101 58-59 1.1620790822305551 1101 60-61 1.063786190879199 1101 62-63 1.1277379285019435 1101 64-65 1.0621401916807542 1101 66-67 1.0055832404378044 1101 68-69 0.9801472166674685 1101 70-71 0.9036982596828125 1101 72-73 0.8118741109461318 1101 74-75 0.6839097982888411 1101 76-77 0.6549936043355657 1101 78-79 0.8054456285099576 1101 80-81 0.7046449179060161 1101 82-83 0.7427813634334619 1101 84-85 0.608005749444473 1101 86-87 0.7433137370320466 1101 88-89 0.6637122486529563 1101 90-91 0.6375106507194701 1101 92-93 0.695308122683354 1101 94-95 0.753298197280845 1101 96-97 0.7458234398617556 1101 98-99 0.7834055693324338 1101 100-101 0.8256727528665273 1101 102-103 0.8927665042470174 1101 104-105 0.8697887165357017 1101 106-107 0.7462337910241281 1101 108-109 0.8401900470007639 1101 110-111 0.7579421993226596 1101 112-113 0.8520894188816541 1101 114-115 0.9073475747184787 1101 116-117 0.8804252866713078 1101 118-119 0.7709258629595794 1101 120-121 0.7619509347344611 1101 122-123 0.9110593500455941 1101 124-125 0.7778605522744115 1102 1 0.40170078809064336 1102 2 0.43597538261698077 1102 3 0.6055992063841664 1102 4 0.6229783456871161 1102 5 0.3320746592187973 1102 6 0.4903458997296468 1102 7 0.31972053924579313 1102 8 0.4500591076169087 1102 9 0.6874343654320327 1102 10-11 0.7105441126780008 1102 12-13 0.7151599549900709 1102 14-15 0.7732785239482141 1102 16-17 0.6447929960766885 1102 18-19 0.7100093655495812 1102 20-21 0.606456565537485 1102 22-23 0.733367962471462 1102 24-25 0.7626245628782158 1102 26-27 0.7007523580869304 1102 28-29 0.635955283216056 1102 30-31 0.726402999500749 1102 32-33 0.7616108418050374 1102 34-35 0.6420046141987967 1102 36-37 0.6499194702000111 1102 38-39 0.6532116097196834 1102 40-41 0.6580748583423528 1102 42-43 0.7838648428620161 1102 44-45 0.7260212920800768 1102 46-47 0.5419728247505375 1102 48-49 0.6836469231010867 1102 50-51 0.7117307136801756 1102 52-53 0.6658697866830607 1102 54-55 0.6292871372024393 1102 56-57 0.729792985931482 1102 58-59 0.8246680743677999 1102 60-61 0.8398137430268449 1102 62-63 0.7060050023858935 1102 64-65 0.7176572493193234 1102 66-67 0.7195610167715394 1102 68-69 0.6483017426275666 1102 70-71 0.5585570549261689 1102 72-73 0.5382986999382702 1102 74-75 0.5975887897762711 1102 76-77 0.6176114482500523 1102 78-79 0.4451753055519987 1102 80-81 0.4556596687020402 1102 82-83 0.41987214256141314 1102 84-85 0.4551229110534152 1102 86-87 0.5275952368046148 1102 88-89 0.6566750142647564 1102 90-91 0.5181452598558671 1102 92-93 0.5902946715169364 1102 94-95 0.6716130975342729 1102 96-97 0.7206412967725377 1102 98-99 0.7338016964362062 1102 100-101 0.7559131843435551 1102 102-103 0.7669815929467418 1102 104-105 0.6956723996859999 1102 106-107 0.7821294307668047 1102 108-109 0.8113721900899833 1102 110-111 0.8483430050930174 1102 112-113 0.7366013429832847 1102 114-115 0.7669007620600894 1102 116-117 0.7765526503726541 1102 118-119 0.8833934696916614 1102 120-121 0.8959328698829445 1102 122-123 0.9067652438658556 1102 124-125 0.9412710402853293 1103 1 0.4720195097371409 1103 2 0.4567832451721614 1103 3 0.5399325237425003 1103 4 0.5456070013704633 1103 5 0.4706217567482156 1103 6 0.45728274783493106 1103 7 0.4457026193743516 1103 8 0.40049802924321654 1103 9 0.6648542975416802 1103 10-11 0.703977113017558 1103 12-13 0.6232327938529636 1103 14-15 0.5611706294770258 1103 16-17 0.618632063925233 1103 18-19 0.6330718954389738 1103 20-21 0.5066941766699529 1103 22-23 0.7604051828622218 1103 24-25 0.7133693887626151 1103 26-27 0.5458992890004879 1103 28-29 0.7719399227435773 1103 30-31 0.7502421183333325 1103 32-33 0.9055483547422369 1103 34-35 0.8003556984764373 1103 36-37 0.6576692996583873 1103 38-39 0.8547855636035919 1103 40-41 0.7818871850603415 1103 42-43 0.83094057659887 1103 44-45 0.7571759837846628 1103 46-47 0.7492673157207506 1103 48-49 0.6030361801514132 1103 50-51 0.531132359852954 1103 52-53 0.5632479577630818 1103 54-55 0.7118441959941819 1103 56-57 0.6046369747598632 1103 58-59 0.6267195957826246 1103 60-61 0.7557408011761808 1103 62-63 0.7937472658122857 1103 64-65 0.6781008971664164 1103 66-67 0.7460606104287244 1103 68-69 0.7912638033618009 1103 70-71 0.733769326989254 1103 72-73 0.7750438684354322 1103 74-75 0.669633958579336 1103 76-77 0.6560698590366414 1103 78-79 0.6596571445260366 1103 80-81 0.6169823579267657 1103 82-83 0.6809054718500072 1103 84-85 0.6340223491072905 1103 86-87 0.6154952958441342 1103 88-89 0.6054378354769732 1103 90-91 0.6108362894174348 1103 92-93 0.6164275624447342 1103 94-95 0.7634625080057837 1103 96-97 0.7889699837600865 1103 98-99 0.6791070347714161 1103 100-101 0.8005664112765878 1103 102-103 0.7291825332964308 1103 104-105 0.8353023982890377 1103 106-107 0.7720640335348286 1103 108-109 0.6763530608770445 1103 110-111 0.6690947087248098 1103 112-113 0.6727152780438885 1103 114-115 0.7262343598306558 1103 116-117 0.7606089204412463 1103 118-119 0.6449968005811613 1103 120-121 0.6847368250210657 1103 122-123 0.8004747306706754 1103 124-125 0.8554284423016476 1104 1 0.5319524977571497 1104 2 0.7008247323178765 1104 3 0.6772585681983223 1104 4 0.7047604538499641 1104 5 0.5363362312120259 1104 6 0.4171493638538095 1104 7 0.4352157399732093 1104 8 0.4198465514081633 1104 9 0.37283555648332367 1104 10-11 0.6352348676001185 1104 12-13 0.5395067853185296 1104 14-15 0.6959460485083397 1104 16-17 0.6754296716429664 1104 18-19 0.6265500463542466 1104 20-21 0.6603004575463558 1104 22-23 0.6641072025116372 1104 24-25 0.7264928262335815 1104 26-27 0.6649993548563771 1104 28-29 0.753705428295909 1104 30-31 0.7367909497525815 1104 32-33 0.8409990030821106 1104 34-35 0.810325949781685 1104 36-37 0.8334193652046125 1104 38-39 0.9369637522982686 1104 40-41 0.8971744736350615 1104 42-43 0.9323204535990683 1104 44-45 0.9364552230768908 1104 46-47 0.8595302541320748 1104 48-49 0.8024424965363153 1104 50-51 0.8959760501775023 1104 52-53 0.9610069479177241 1104 54-55 0.8762926993726481 1104 56-57 0.794546572584764 1104 58-59 0.9371630185547204 1104 60-61 0.9147159369652442 1104 62-63 0.7339236935328586 1104 64-65 0.835731561237381 1104 66-67 0.8628699132440154 1104 68-69 0.7911845801722706 1104 70-71 0.8120521604853366 1104 72-73 0.8404370631892562 1104 74-75 0.7345582655957763 1104 76-77 0.6314518335159391 1104 78-79 0.6674473388112325 1104 80-81 0.7542691778718122 1104 82-83 0.7972314357923587 1104 84-85 0.8457670966356332 1104 86-87 0.7849493112985897 1104 88-89 0.7940814106053296 1104 90-91 0.8334617097906438 1104 92-93 0.7746045310506737 1104 94-95 0.8756317060296972 1104 96-97 0.7905771564779904 1104 98-99 0.8478495898560467 1104 100-101 0.9062351788631027 1104 102-103 0.8675973159823194 1104 104-105 0.8832166865396474 1104 106-107 0.8774378431225394 1104 108-109 0.9652062290820673 1104 110-111 0.8508644463954127 1104 112-113 0.8641123106064263 1104 114-115 0.84378041250298 1104 116-117 0.7828244446768302 1104 118-119 0.8054740802994047 1104 120-121 0.9196054538766809 1104 122-123 0.9802886619782356 1104 124-125 0.8642624730743087 1105 1 0.44262186854045993 1105 2 0.4576009904455134 1105 3 0.4591535560705644 1105 4 0.4329625899662304 1105 5 0.4720050924256185 1105 6 0.382612256986846 1105 7 0.34745866204981013 1105 8 0.49837430257507265 1105 9 0.49510986805462665 1105 10-11 0.6292423277008083 1105 12-13 0.5507774483706598 1105 14-15 0.4714286110325432 1105 16-17 0.44127652555951613 1105 18-19 0.4370767376161453 1105 20-21 0.3752466469839959 1105 22-23 0.42040548424254354 1105 24-25 0.5417511271243143 1105 26-27 0.46238755525995856 1105 28-29 0.5826477144294913 1105 30-31 0.4531333602211802 1105 32-33 0.74619024459777 1105 34-35 0.7658127417345639 1105 36-37 0.6365792070341669 1105 38-39 0.6965192036751233 1105 40-41 0.6701130093355161 1105 42-43 0.7504150195475745 1105 44-45 0.8277833655933478 1105 46-47 0.5510489757235675 1105 48-49 0.7332146475320158 1105 50-51 0.6827573020883975 1105 52-53 0.6812707521614243 1105 54-55 0.8473962745137911 1105 56-57 0.8785386946369016 1105 58-59 0.7569452794136495 1105 60-61 0.7566504674477059 1105 62-63 0.8629434342637907 1105 64-65 0.8810684887358065 1105 66-67 0.810453278850094 1105 68-69 0.7490268581527388 1105 70-71 0.710341025367832 1105 72-73 0.6875360827771182 1105 74-75 0.6867094141659216 1105 76-77 0.7302388027990752 1105 78-79 0.6736434518134118 1105 80-81 0.76320957859744 1105 82-83 0.7390841803348707 1105 84-85 0.7589743794060588 1105 86-87 0.7608002927298685 1105 88-89 0.6497442588962343 1105 90-91 0.6513634036003921 1105 92-93 0.728476496562994 1105 94-95 0.707641347088785 1105 96-97 0.6384777292343564 1105 98-99 0.5232218305360163 1105 100-101 0.6159320358370763 1105 102-103 0.6518689370255082 1105 104-105 0.6719602914396674 1105 106-107 0.7225999882072252 1105 108-109 0.5861413914309814 1105 110-111 0.5742987934327779 1105 112-113 0.5661944124796037 1105 114-115 0.4946471905955576 1105 116-117 0.5985795760439068 1105 118-119 0.6211576171209146 1105 120-121 0.7118286556051423 1105 122-123 0.6681137674591646 1105 124-125 0.7013349440797896 1106 1 -0.3966549269844606 1106 2 -0.39690904851163467 1106 3 -0.18936807014060264 1106 4 -0.20118734510518976 1106 5 -0.1564186414670985 1106 6 -0.23953588006239812 1106 7 -0.3365689076235512 1106 8 -0.3444635757587591 1106 9 -0.3071826102192716 1106 10-11 -0.2279372747365187 1106 12-13 -0.22702696677269074 1106 14-15 -0.2677682723956778 1106 16-17 -0.21884858681686126 1106 18-19 -0.35713712713263135 1106 20-21 -0.36352820068357516 1106 22-23 -0.3874433820045837 1106 24-25 -4.232378580197633 1106 26-27 -1.291007460223014 1106 28-29 -0.9181451634048869 1106 30-31 -0.7691296504670646 1106 32-33 -5.869641090933143 1106 34-35 -1.4043688085601147 1106 36-37 -0.5919793785217209 1106 38-39 -0.12558897732576924 1106 40-41 -0.6861488526163413 1106 42-43 -0.3382387113484313 1106 44-45 -0.1778279619463632 1106 46-47 -0.3765506646720027 1106 48-49 -0.09570978559155208 1106 50-51 0.03160357119238455 1106 52-53 -0.12766067327059716 1106 54-55 -0.29507575625235916 1106 56-57 -0.02006658955049545 1106 58-59 0.13749042974828285 1106 60-61 0.12029296070512174 1106 62-63 0.005747599681825477 1106 64-65 0.057009953124975254 1106 66-67 -0.07111824247644805 1106 68-69 -0.07285407282318346 1106 70-71 0.05669503595618508 1106 72-73 0.018164891087977253 1106 74-75 -0.017876791008387016 1106 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-1.7632378874965013 2216 104-105 -1.7819758860115122 2216 106-107 -1.8502686244348077 2216 108-109 -1.9423984466312092 2216 110-111 -1.697655516970709 2216 112-113 -1.8175539715443492 2216 114-115 -1.9852075416627244 2216 116-117 -1.888493466073534 2216 118-119 -2.0763503777079606 2216 120-121 -2.0621975061965863 2216 122-123 -2.101841835725157 2216 124-125 -2.1210054995481613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62697.0 3 10393.0 4 6736.0 5 5723.0 6 6202.0 7 6949.0 8 7488.0 9 8202.0 10 9414.0 11 10552.0 12 11038.0 13 11206.0 14 11301.0 15 11068.0 16 10352.0 17 9523.0 18 8472.0 19 8094.0 20 7790.0 21 8285.0 22 9266.0 23 12324.0 24 18008.0 25 25349.0 26 29430.0 27 29767.0 28 33965.0 29 40979.0 30 48497.0 31 58420.0 32 72754.0 33 93552.0 34 124381.0 35 173923.0 36 269035.0 37 345777.0 38 109938.0 39 304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.097877950451355 18.009253388962517 9.314414315829941 22.578454344756192 2 19.00167501084154 20.891779709151418 37.63505607204457 22.471489207962467 3 17.14936826710299 28.51980772994186 30.890991770276422 23.439832232678732 4 10.752261090939829 16.08554656130638 37.73603149228818 35.42616085546561 5 10.691084683349352 43.12591914957676 34.86335792118763 11.319638245886262 6 30.598941144592125 37.52782042998052 19.123838837204453 12.749399588222898 7 27.908285532813437 34.78200704863465 21.58281045634322 15.72689696220869 8 26.85527916398159 34.453277935360155 20.032145646644537 18.65929725401372 9 27.888388315651795 13.482416794093787 18.680922292518634 39.94827259773579 10-11 23.722710571385893 27.24250116088271 29.67612819850689 19.358660069224506 12-13 24.68043751270173 26.280522509346447 28.74305132164572 20.295988656306108 14-15 22.431128665106648 27.627538517256085 25.719067427742964 24.222265389894307 16-17 22.329809836551554 29.831375304372397 26.181946298446633 21.65686856062942 18-19 21.580846027156777 28.89461325634966 27.82607109699126 21.698469619502298 20-21 23.194342234700336 28.12855904622338 27.237881945188303 21.439216773887985 22-23 22.70664178362825 28.447038214355146 27.034285454345795 21.81203454767081 24-25 22.3785321725597 28.279932419444116 27.45633781940382 21.885197588592366 26-27 22.308952287431087 28.346468774281558 27.149543694930443 22.195035243356916 28-29 22.005151301011818 28.413880431569826 27.38280963896021 22.198158628458145 30-31 22.37110993575798 27.960898375088767 27.6428582388057 22.025133450347553 32-33 21.959278132347805 28.20735659310151 27.547273484176937 22.286091790373746 34-35 22.428394670730416 28.294302549704973 27.409661399966765 21.867641379597842 36-37 22.43261723951856 28.06163687188359 27.235679860672047 22.270066027925807 38-39 22.084209782767918 28.16941774807934 27.57047927236893 22.175893196783814 40-41 22.237713489020564 28.134781355050958 27.627149654515158 22.00035550141332 42-43 22.136328580957144 28.013862255201676 27.72306292034906 22.126746243492114 44-45 22.00781466896218 28.201679485089915 27.644224993218547 22.146280852729358 46-47 22.15080322488473 28.148580464197426 27.204529591406327 22.496086719511514 48-49 22.120046863316194 28.10458613768748 27.496879976585713 22.278487022410612 50-51 22.08662538835924 27.984330146172454 27.54779872830081 22.3812457371675 52-53 22.22260821735386 28.04165852458186 27.462839524936495 22.27289373312779 54-55 22.003336742643228 27.978902615229224 27.727503403761204 22.290257238366344 56-57 22.149738685818225 28.204648679851697 27.517875696492833 22.127736937837245 58-59 22.45436833618486 27.898931473616866 27.278601391313412 22.368098798884866 60-61 22.391750989569175 27.889740296171624 27.659012107005772 22.059496607253433 62-63 21.921985298204657 28.110148824412207 27.642351268226705 22.32551460915643 64-65 21.826175592649 28.148131636153305 27.597104924066645 22.428587847131052 66-67 22.0850951481314 28.37022167745805 27.299526560823644 22.2451566135869 68-69 22.125817530825312 28.011663130980946 27.588915162070275 22.273604176123467 70-71 21.924480081892277 27.934999163360153 27.65722894461213 22.48329181013544 72-73 22.06391734372037 28.092858042355846 27.386435187396597 22.456789426527187 74-75 21.723642496701938 28.40574692245986 27.54771515279068 22.322895428047527 76-77 21.637830160767646 28.198258287781442 27.708723788481727 22.45518776296919 78-79 21.942127287544093 28.104929935254702 27.48845281467618 22.464489962525022 80-81 21.878087636254048 28.160892753700388 27.400738962817655 22.560280647227906 82-83 22.125624618773028 28.11956204240098 27.343226875897248 22.411586462928742 84-85 21.750453419718294 27.980650232101684 27.527708178635063 22.74118816954496 86-87 21.66024087038033 28.11378030782449 27.552017684594244 22.673961137200934 88-89 21.866553107819435 27.89361558612548 27.648095722928765 22.591735583126322 90-91 21.844966832700983 27.966891178720125 27.63269959291389 22.555442395665004 92-93 21.94055412641975 27.87639876304967 27.54663604977214 22.63641106075844 94-95 21.63272880230831 27.736246646573854 27.687119514414345 22.943905036703498 96-97 21.687102054192135 27.87969899562922 27.693409551336273 22.739789398842372 98-99 21.63354564829595 27.9417954770602 27.502748746345862 22.92191012829799 100-101 21.385143154908377 28.017659149465885 27.667805344063922 22.929392351561823 102-103 21.44902841020448 27.827276656514066 27.73573822904698 22.987956704234477 104-105 21.284550063558484 27.684256632098148 27.96914800948036 23.062045294863008 106-107 21.321315844401425 27.905314508095564 27.909946417493103 22.86342323000991 108-109 21.345658086527063 27.983365636113884 27.59619546101303 23.074780816346028 110-111 21.37020501487138 28.21164779059985 27.510489866791577 22.907657327737194 112-113 21.406280396861423 27.88553063077251 27.704017943991033 23.004171028375037 114-115 21.084770021355997 27.97867237317904 27.707329330784624 23.229228274680334 116-117 21.065608581100193 28.06102789893955 27.745101814005718 23.12826170595454 118-119 21.12077288532448 27.921761920077632 27.60528469488695 23.352180499710936 120-121 21.106256611328845 27.89501658513539 27.825740570153474 23.172986233382296 122-123 21.12447192583614 27.819929786002767 27.669566340126515 23.386031948034578 124-125 21.26894448201432 27.640966168582732 27.403175864081447 23.6869134853215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 51.0 1 75.5 2 106.5 3 119.0 4 122.5 5 117.5 6 138.5 7 168.0 8 163.0 9 169.5 10 180.0 11 183.0 12 196.0 13 224.5 14 274.0 15 332.0 16 416.5 17 546.0 18 751.5 19 973.5 20 1342.0 21 1967.0 22 2765.5 23 3956.0 24 5819.5 25 8175.0 26 11328.0 27 14590.5 28 16946.0 29 19651.0 30 23687.0 31 27912.5 32 31626.0 33 36065.0 34 41177.5 35 44842.0 36 48969.0 37 53166.0 38 54453.5 39 55459.5 40 57005.5 41 58915.5 42 60894.0 43 62624.0 44 65398.0 45 67595.0 46 67965.0 47 67138.0 48 65228.5 49 63670.5 50 62179.0 51 58507.0 52 54422.0 53 51682.5 54 48627.0 55 44438.5 56 39954.0 57 36669.5 58 33396.5 59 29381.5 60 25401.0 61 21179.0 62 17428.5 63 13785.0 64 10543.5 65 8358.5 66 6350.5 67 4832.5 68 3687.0 69 2495.0 70 1818.0 71 1414.0 72 1185.0 73 1046.0 74 816.5 75 644.5 76 443.5 77 289.5 78 208.5 79 121.0 80 67.0 81 51.5 82 37.5 83 21.0 84 14.0 85 10.5 86 7.5 87 4.0 88 2.5 89 2.0 90 2.5 91 2.5 92 1.0 93 0.5 94 1.0 95 2.0 96 2.0 97 1.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.736961498511424E-4 2 5.789871661704747E-5 3 0.0 4 5.789871661704747E-5 5 8.105820326386646E-4 6 3.473922997022848E-4 7 6.368858827875221E-4 8 8.684807492557121E-4 9 0.0013316704821920917 10-11 0.0011579743323409492 12-13 0.0015632653486602815 14-15 0.0022291005897563275 16-17 0.0019975057232881374 18-19 0.0019975057232881374 20-21 0.002373847381298946 22-23 0.08571904995153878 24-25 0.0017369614985114242 26-27 0.0011579743323409492 28-29 0.0010132275407983306 30-31 7.816326743301408E-4 32-33 0.0013316704821920917 34-35 7.526833160216171E-4 36-37 3.473922997022848E-4 38-39 8.684807492557121E-4 40-41 9.263794658727595E-4 42-43 0.0010421768991068544 44-45 0.0010132275407983306 46-47 0.0013027211238835679 48-49 2.0264550815966614E-4 50-51 9.263794658727595E-4 52-53 9.553288241812832E-4 54-55 0.001186923690649473 56-57 0.0010711262574153782 58-59 5.210884495534272E-4 60-61 7.816326743301408E-4 62-63 0.0010421768991068544 64-65 8.395313909471883E-4 66-67 2.894935830852373E-4 68-69 1.1579743323409494E-4 70-71 4.052910163193323E-4 72-73 8.68480749255712E-5 74-75 2.0264550815966614E-4 76-77 1.736961498511424E-4 78-79 8.68480749255712E-5 80-81 2.0264550815966614E-4 82-83 2.8949358308523734E-5 84-85 8.68480749255712E-5 86-87 5.789871661704747E-5 88-89 1.4474679154261865E-4 90-91 5.789871661704747E-5 92-93 1.736961498511424E-4 94-95 1.4474679154261865E-4 96-97 1.736961498511424E-4 98-99 1.736961498511424E-4 100-101 2.3159486646818987E-4 102-103 6.079365244789984E-4 104-105 2.605442247767136E-4 106-107 2.605442247767136E-4 108-109 3.7634165801080853E-4 110-111 6.368858827875221E-4 112-113 1.1579743323409494E-4 114-115 2.605442247767136E-4 116-117 5.500378078619509E-4 118-119 8.974301075642357E-4 120-121 2.894935830852373E-4 122-123 3.1844294139376107E-4 124-125 1.1579743323409494E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1727154.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.57542280747393 #Duplication Level Percentage of deduplicated Percentage of total 1 52.78413258239972 18.77817834144631 2 15.433911091548314 10.981358253095854 3 10.520631040916065 11.228276924460706 4 7.027188346941987 9.999807863608599 5 4.487950394371077 7.983036640936021 6 2.896311471266853 6.18225031034731 7 1.8255138158139383 4.546039808692616 8 1.1828801119070376 3.3665168089315953 9 0.7580784578808761 2.4272065494314985 >10 2.865678689204372 16.67810258790784 >50 0.151314978243763 3.67662323487832 >100 0.06542037331749768 3.9475527953174243 >500 9.886461885788389E-4 0.20504988094597149 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.789871661704747E-5 0.0 20-21 0.0 0.0 0.0 5.789871661704747E-5 0.0 22-23 0.0 0.0 0.0 1.1579743323409494E-4 0.0 24-25 0.0 0.0 0.0 1.736961498511424E-4 0.0 26-27 0.0 0.0 0.0 2.3159486646818987E-4 0.0 28-29 0.0 0.0 0.0 5.210884495534272E-4 0.0 30-31 0.0 5.789871661704747E-5 0.0 0.002055404439905185 0.0 32-33 0.0 1.7369614985114238E-4 0.0 0.0071794408605138854 0.0 34-35 0.0 2.894935830852373E-4 0.0 0.014706274020730055 0.0 36-37 0.0 2.894935830852373E-4 0.0 0.026083371835979884 0.0 38-39 0.0 2.894935830852373E-4 0.0 0.047245352759510736 0.0 40-41 0.0 2.894935830852373E-4 0.0 0.07121542143896839 0.0 42-43 0.0 2.894935830852373E-4 0.0 0.10022267846410916 0.0 44-45 0.0 3.1844294139376107E-4 0.0 0.1369883635159343 0.0 46-47 0.0 3.473922997022848E-4 0.0 0.18374157718420014 0.0 48-49 0.0 3.473922997022848E-4 0.0 0.23414240999933994 0.0 50-51 0.0 3.473922997022848E-4 0.0 0.28173515505855296 0.0 52-53 0.0 3.473922997022848E-4 0.0 0.3365941890532055 0.0 54-55 0.0 3.473922997022848E-4 0.0 0.4027434727881822 0.0 56-57 0.0 3.473922997022848E-4 0.0 0.4813409805958241 0.0 58-59 0.0 3.473922997022848E-4 0.0 0.5727920034924506 0.0 60-61 0.0 3.473922997022848E-4 0.0 0.6669353167117698 0.0 62-63 0.0 3.473922997022848E-4 0.0 0.7658552740519953 0.0 64-65 0.0 3.473922997022848E-4 0.0 0.883852858517538 0.0 66-67 0.0 3.473922997022848E-4 0.0 1.0235045629978567 0.0 68-69 0.0 3.473922997022848E-4 0.0 1.1952032071257108 0.0 70-71 5.789871661704747E-5 3.473922997022848E-4 0.0 1.3734444062312914 0.0 72-73 5.789871661704747E-5 3.473922997022848E-4 0.0 1.5518303521284147 0.0 74-75 5.789871661704747E-5 3.473922997022848E-4 0.0 1.7442277874468632 0.0 76-77 5.789871661704747E-5 3.473922997022848E-4 0.0 1.9656614291487615 0.0 78-79 5.789871661704747E-5 3.473922997022848E-4 0.0 2.233616689652457 0.0 80-81 5.789871661704747E-5 3.473922997022848E-4 0.0 2.5128911492547856 0.0 82-83 5.789871661704747E-5 3.473922997022848E-4 0.0 2.7937867729223913 0.0 84-85 5.789871661704747E-5 3.473922997022848E-4 0.0 3.1037765016900636 0.0 86-87 5.789871661704747E-5 3.473922997022848E-4 0.0 3.4360861857136076 0.0 88-89 5.789871661704747E-5 3.473922997022848E-4 0.0 3.820215221109409 0.0 90-91 5.789871661704747E-5 3.473922997022848E-4 0.0 4.237346524976927 0.0 92-93 5.789871661704747E-5 3.473922997022848E-4 0.0 4.653233006437179 0.0 94-95 5.789871661704747E-5 3.473922997022848E-4 0.0 5.091960531602856 0.0 96-97 5.789871661704747E-5 3.473922997022848E-4 0.0 5.5721435378663395 0.0 98-99 5.789871661704747E-5 3.473922997022848E-4 0.0 6.09925345394794 0.0 100-101 5.789871661704747E-5 3.473922997022848E-4 0.0 6.6617105365242475 0.0 102-103 5.789871661704747E-5 3.473922997022848E-4 0.0 7.2176540134811376 0.0 104-105 5.789871661704747E-5 3.473922997022848E-4 0.0 7.794962116869717 0.0 106-107 5.789871661704747E-5 3.473922997022848E-4 0.0 8.436595694419836 0.0 108-109 5.789871661704747E-5 3.473922997022848E-4 0.0 9.100780822092297 0.0 110-111 5.789871661704747E-5 3.473922997022848E-4 0.0 9.802484318132604 0.0 112-113 5.789871661704747E-5 3.473922997022848E-4 0.0 10.513393710115022 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2745 0.0 61.553333 1 GTACATG 14370 0.0 52.373222 1 TACATGG 14395 0.0 51.04237 2 ACATGGG 14735 0.0 48.814823 3 CATGGGG 4235 0.0 45.09487 4 CATGGGA 10160 0.0 44.03517 4 GTATCAA 3805 0.0 43.936676 2 ATGGGAT 4045 0.0 38.976505 5 TCAACGC 4525 0.0 37.340084 5 CAACGCA 4530 0.0 37.0362 6 ATCAACG 4530 0.0 36.6422 4 ATGGGAG 3960 0.0 36.057167 5 AACGCAG 4660 0.0 35.87533 7 AGTACAT 8475 0.0 31.940908 2 GAGTACA 8815 0.0 31.85629 1 TATCAAC 5330 0.0 31.812162 3 ATGGGGG 2560 0.0 31.606361 5 ATGGGGA 2300 0.0 30.523176 5 ACGCAGA 5700 0.0 29.225283 8 CGCAGAG 5730 0.0 28.864613 9 >>END_MODULE