FastQCFastQC Report
Thu 26 May 2016
SRR936225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727154
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41890.24253772390881184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.15945306556334873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27370.1584687873808589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.071.949871
GTATCAA64400.051.8576352
GTACATG128050.046.8476371
TACATGG130500.045.890542
ACATGGG130650.045.245523
CATGGGA91550.042.070894
ATGGGAG32100.040.0575565
TCAACGC88300.039.5068745
CAACGCA88450.039.483166
ATCAACG88600.039.3059124
AACGCAG91950.038.0449687
CATGGGG40450.037.822514
TATCAAC94350.036.72123
ATGGGAT38150.033.3929635
ACGCAGA107750.032.1901448
CGCAGAG108450.031.8177939
AGTACAT80600.030.5035762
GAGTACA85450.029.7682691
ATGGGGG21850.027.789745
ATGGGAC17800.026.7550495
Produced by FastQC (version 0.11.5)