FastQCFastQC Report
Thu 26 May 2016
SRR936224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211580
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9960.4707439266471311No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6060.28641648549012194No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5960.28169014084507044No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5400.2552226108327819No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.2037054542017204No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT4020.18999905473107098No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3750.1772379241894319No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3060.14462614613857644No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2930.13848189810000944No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT2880.13611872577748368No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2860.1351734568484734No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2480.11721334719727763No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.10965119576519519No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2300.10870592683618491No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT2240.10587012004915398No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2130.10067114093959732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGT251.7230428E-595.193387
CGTCACT552.0008883E-1186.539441
TCCCGCT250.002055087871.3950358
CTAGGGC250.002055087871.3950353
ATCCCGT201.760977E-459.56625720-21
TCCCGTT201.760977E-459.56625720-21
GCCGGTC401.7692696E-459.495878
CGGCCGT150.004235870759.4958652-53
CGAATGC150.004235870759.4958642-43
CATTCCG551.3075765E-554.087159
GTCACTA1006.91216E-1153.5462762
GTACATA1350.052.8852161
TCTTGCG453.1660957E-452.885212
CTTGCGC453.1660957E-452.885213
ATGGGCG709.109863E-750.9964565
ACATGGA4200.048.163323
GTACATG42250.047.737511
AACCGCG255.3305866E-447.59669102-103
TCTAGCG255.3305866E-447.5966928-29
GTTCCGA255.3305866E-447.5966980-81
Produced by FastQC (version 0.11.5)