##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 211580 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.47064939975423 31.0 22.0 31.0 7.0 34.0 2 25.62459589753285 31.0 23.0 31.0 7.0 34.0 3 25.959528310804423 31.0 23.0 31.0 7.0 34.0 4 28.13048964930523 35.0 22.0 37.0 7.0 37.0 5 29.66244446545042 35.0 28.0 37.0 7.0 37.0 6 28.776344645051516 35.0 26.0 37.0 8.0 37.0 7 29.106555440022685 35.0 28.0 37.0 8.0 37.0 8 28.59053313167596 35.0 26.0 37.0 7.0 37.0 9 29.07962472823518 37.0 18.0 39.0 7.0 39.0 10-11 29.530811040741092 37.0 26.0 39.0 7.0 39.0 12-13 29.477372625011817 37.0 24.5 39.0 7.0 39.0 14-15 30.337139616220814 37.5 26.0 40.0 7.0 41.0 16-17 30.545467435485392 37.5 26.0 40.0 7.0 41.0 18-19 30.7245699026373 37.5 26.5 40.0 7.0 41.0 20-21 31.0961291237357 38.0 27.0 40.0 7.0 41.0 22-23 31.342697797523396 37.5 27.0 40.0 7.0 41.0 24-25 31.627925607335285 37.0 28.5 40.0 7.0 41.0 26-27 31.92297712449192 37.0 29.5 40.0 4.5 41.0 28-29 31.88493713961622 37.0 29.5 40.0 2.0 41.0 30-31 31.783360903677096 37.0 29.5 40.0 2.0 41.0 32-33 31.585641364968332 37.0 29.5 40.0 2.0 41.0 34-35 31.435887134889875 37.0 29.0 40.0 2.0 41.0 36-37 31.17824936194347 36.5 29.0 40.0 2.0 41.0 38-39 30.818652519141693 36.0 28.0 40.0 2.0 41.0 40-41 30.870072785707535 36.0 28.0 40.0 2.0 41.0 42-43 30.654010303431328 36.0 27.5 40.0 2.0 41.0 44-45 30.574366669817564 36.0 28.0 40.0 2.0 41.0 46-47 30.51567964835996 36.0 28.0 40.0 2.0 41.0 48-49 30.35247896776633 36.0 28.0 40.0 2.0 41.0 50-51 30.221814443709235 35.5 28.0 40.0 2.0 41.0 52-53 30.013971074770772 35.0 28.0 40.0 2.0 41.0 54-55 29.750909821344173 35.0 28.0 40.0 2.0 41.0 56-57 29.516133377445883 35.0 28.0 40.0 2.0 41.0 58-59 29.211792229889404 35.0 27.0 40.0 2.0 41.0 60-61 28.88097882597599 34.0 26.5 39.0 2.0 41.0 62-63 28.545020795916436 34.0 26.0 39.0 2.0 41.0 64-65 28.187323943661973 34.0 26.0 39.0 2.0 41.0 66-67 27.860258531052082 34.0 24.5 39.0 2.0 40.5 68-69 27.547785707533794 34.0 23.5 38.0 2.0 40.0 70-71 27.17587910010398 33.0 22.5 37.5 2.0 40.0 72-73 26.78174449380849 33.0 21.5 37.0 2.0 40.0 74-75 26.365067586728422 33.0 19.0 36.5 2.0 39.0 76-77 25.975283580678703 32.5 16.5 36.0 2.0 39.0 78-79 25.566341336610265 32.0 10.0 36.0 2.0 39.0 80-81 25.205929199357215 32.0 6.0 35.0 2.0 37.5 82-83 24.851143775404104 32.0 2.0 35.0 2.0 37.0 84-85 24.768728140656016 32.0 2.0 35.0 2.0 37.0 86-87 24.532789015975045 32.0 2.0 35.0 2.0 36.0 88-89 24.261092730881934 32.0 2.0 35.0 2.0 36.0 90-91 23.98838028169014 32.0 2.0 35.0 2.0 36.0 92-93 23.82569004631818 32.0 2.0 35.0 2.0 35.0 94-95 23.67616977029965 32.0 2.0 35.0 2.0 35.0 96-97 23.51420266565838 32.0 2.0 35.0 2.0 35.0 98-99 23.330655071367804 32.0 2.0 35.0 2.0 35.0 100-101 23.169089706021364 31.5 2.0 35.0 2.0 35.0 102-103 22.33449758956423 29.5 2.0 34.0 2.0 35.0 104-105 22.811444843557993 31.0 2.0 35.0 2.0 35.0 106-107 22.896478873239438 31.0 2.0 35.0 2.0 35.0 108-109 22.829159183287643 31.0 2.0 35.0 2.0 35.0 110-111 22.697022402873618 31.0 2.0 35.0 2.0 35.0 112-113 22.589897438321202 31.0 2.0 35.0 2.0 35.0 114-115 22.412383022970033 31.0 2.0 35.0 2.0 35.0 116-117 22.238571698648265 31.0 2.0 35.0 2.0 35.0 118-119 22.041948671897153 31.0 2.0 35.0 2.0 35.0 120-121 21.773920030248604 31.0 2.0 35.0 2.0 35.0 122-123 21.420098780603084 30.0 2.0 35.0 2.0 35.0 124-125 19.717333868985726 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3896335687854986 1101 2 2.0019868202540465 1101 3 1.1987206249275104 1101 4 1.3830125211014312 1101 5 1.4614691612049988 1101 6 1.1914170205323877 1101 7 1.2568928065175804 1101 8 1.0938689114776992 1101 9 1.5462911053412363 1101 10-11 1.2730110884555792 1101 12-13 1.6338687623956467 1101 14-15 1.5716893979769004 1101 16-17 1.1719031475010482 1101 18-19 1.2062955678370528 1101 20-21 1.020280154594527 1101 22-23 1.2361413782677673 1101 24-25 1.251642964149024 1101 26-27 1.0780621519221647 1101 28-29 1.5531198357860951 1101 30-31 1.2953447320090987 1101 32-33 1.7962999554445318 1101 34-35 1.4711454742098375 1101 36-37 1.447823823646786 1101 38-39 1.8857627135150459 1101 40-41 1.6613413838775202 1101 42-43 1.5636552959231587 1101 44-45 1.7250258291418277 1101 46-47 1.486220994189857 1101 48-49 1.616894430288749 1101 50-51 1.8146121959280066 1101 52-53 1.6707411827426455 1101 54-55 1.480658107681613 1101 56-57 1.54411767271068 1101 58-59 1.3453071367770377 1101 60-61 1.3817028495803108 1101 62-63 1.3744054354643396 1101 64-65 1.2817178843120622 1101 66-67 1.060558783705872 1101 68-69 1.076327416755639 1101 70-71 1.0959017568598206 1101 72-73 1.495363591366587 1101 74-75 1.2655634182592088 1101 76-77 1.17764354644682 1101 78-79 1.1456244827304367 1101 80-81 1.4830714370413212 1101 82-83 1.3351900110324912 1101 84-85 1.2789169763645098 1101 86-87 1.2749912917944748 1101 88-89 1.406592671692895 1101 90-91 1.3106762524763695 1101 92-93 1.4616890479069333 1101 94-95 1.1746993296050618 1101 96-97 1.2978955308460876 1101 98-99 1.1654199563323004 1101 100-101 1.339858226682633 1101 102-103 1.4130442467072903 1101 104-105 1.5381105561425379 1101 106-107 1.4036687701876254 1101 108-109 1.5981421579459578 1101 110-111 1.690998181241408 1101 112-113 1.451992458199559 1101 114-115 1.5006196912395353 1101 116-117 1.5139611714424426 1101 118-119 1.6742973947692867 1101 120-121 1.7315096309307911 1101 122-123 2.0050920203111424 1101 124-125 1.8001394638214094 1102 1 0.6920956899976183 1102 2 0.46505500207222994 1102 3 0.39142895826084256 1102 4 0.42051252110143267 1102 5 0.26127976726560576 1102 6 0.5842200508354196 1102 7 0.3947715943963672 1102 8 0.24898254784133655 1102 9 0.5745108023109324 1102 10-11 0.536363361182854 1102 12-13 0.22439906542595267 1102 14-15 0.31652841312841673 1102 16-17 -0.047509731286833556 1102 18-19 0.36036753753402095 1102 20-21 0.27037485156422747 1102 22-23 0.2890296358435265 1102 24-25 0.6081297065732691 1102 26-27 0.6224750307100457 1102 28-29 0.5169455933618572 1102 30-31 0.6460549592818268 1102 32-33 0.7149079099899822 1102 34-35 0.71243335299771 1102 36-37 0.6495283691013327 1102 38-39 0.6449956680604991 1102 40-41 0.5065591869078219 1102 42-43 0.8504450686504335 1102 44-45 0.8141356776266804 1102 46-47 0.7100372820686474 1102 48-49 0.49085276362207964 1102 50-51 0.5542428777461836 1102 52-53 0.6321995160759819 1102 54-55 1.018110759196766 1102 56-57 0.9440703242258337 1102 58-59 0.6936499398073401 1102 60-61 0.7834547435197052 1102 62-63 0.6036667991007043 1102 64-65 0.8404773540090318 1102 66-67 0.4238163594634514 1102 68-69 0.2726342349374562 1102 70-71 0.3875684235264849 1102 72-73 0.42642419742719184 1102 74-75 0.49349902431981363 1102 76-77 0.7238556676589383 1102 78-79 0.9498858463668007 1102 80-81 0.8211869673443495 1102 82-83 0.8401142534567363 1102 84-85 0.4046272036372365 1102 86-87 0.5821409130065938 1102 88-89 0.588694944420169 1102 90-91 0.4745493585369758 1102 92-93 0.6034977600281444 1102 94-95 0.3863944053626369 1102 96-97 0.4387099247854813 1102 98-99 0.44165101693836206 1102 100-101 0.5717711054705141 1102 102-103 0.45442682246486754 1102 104-105 0.34956888947587217 1102 106-107 0.34850778533914095 1102 108-109 0.336873218552018 1102 110-111 0.2364527266959513 1102 112-113 0.19758904910865382 1102 114-115 0.023015524572869595 1102 116-117 0.10984185326062246 1102 118-119 0.0034167129511040173 1102 120-121 0.308119479415641 1102 122-123 0.10111474758386763 1102 124-125 0.02471332745777488 1103 1 -0.2236783647492473 1103 2 -0.04576620089398986 1103 3 -0.0567080004501328 1103 4 -0.04927599853602871 1103 5 0.13467535455847468 1103 6 -0.7007657589238043 1103 7 -0.33782018441897677 1103 8 -0.41215450241957896 1103 9 -0.7324286831784015 1103 10-11 -0.28545756713354464 1103 12-13 -0.315338186244837 1103 14-15 -0.29258080595059255 1103 16-17 -0.18790614252916527 1103 18-19 0.0023019877766259356 1103 20-21 -0.7216099511456484 1103 22-23 -0.33365186191350205 1103 24-25 -0.5210014844914568 1103 26-27 -0.6268566547243601 1103 28-29 -0.5158567503166189 1103 30-31 -0.5408854417069122 1103 32-33 -0.456555973558487 1103 34-35 -0.5810070937659937 1103 36-37 -0.7697932458396188 1103 38-39 -0.780697822738734 1103 40-41 -0.5562898472403077 1103 42-43 -0.44690456057230676 1103 44-45 -0.259273642157261 1103 46-47 -0.3674185450850658 1103 48-49 -0.7275258869317938 1103 50-51 -0.2952490200840785 1103 52-53 -0.18074106801264023 1103 54-55 -0.25893309322473357 1103 56-57 -0.22934815055215907 1103 58-59 -0.3753924478151056 1103 60-61 -0.2290647108426498 1103 62-63 -0.15498467027584084 1103 64-65 -0.369551941971924 1103 66-67 -0.040611911158173086 1103 68-69 -0.0801397282594678 1103 70-71 -0.22728555431843134 1103 72-73 -0.10727991316513652 1103 74-75 -0.21588484458067114 1103 76-77 -0.4049093236438175 1103 78-79 -0.5196417559402597 1103 80-81 -0.6937082079737884 1103 82-83 -0.5589565146472673 1103 84-85 -0.4688690885901714 1103 86-87 -0.28061199370401724 1103 88-89 -0.2731561953765933 1103 90-91 -0.02667741066562357 1103 92-93 -0.10605547626225231 1103 94-95 -0.21171966586321744 1103 96-97 -0.21957992232611545 1103 98-99 -0.2568704515226834 1103 100-101 -0.4801285557947068 1103 102-103 -0.45108906144980665 1103 104-105 -0.4558188245221153 1103 106-107 -0.5417192585132788 1103 108-109 -0.4111950777035851 1103 110-111 -0.5470682840153884 1103 112-113 -0.5176402834922804 1103 114-115 -0.4947588963737566 1103 116-117 -0.45898539200166866 1103 118-119 -0.41944788468690675 1103 120-121 -0.19791823915984352 1103 122-123 -0.18884680144112664 1103 124-125 -0.15704992439611232 1104 1 0.6147432179083054 1104 2 0.795517522008435 1104 3 0.990844162939208 1104 4 0.5023838661306712 1104 5 0.9692542489242975 1104 6 0.7802328100060727 1104 7 0.778237835757345 1104 8 0.19332066586366636 1104 9 0.5974241462769072 1104 10-11 0.4595425212041171 1104 12-13 1.0362810430974 1104 14-15 1.158979237158185 1104 16-17 1.0953132352203454 1104 18-19 0.4795594567259407 1104 20-21 0.5476741604424831 1104 22-23 0.5674187905484693 1104 24-25 1.1025475694121845 1104 26-27 1.1814064501677812 1104 28-29 1.1315920580083194 1104 30-31 1.0597179045237226 1104 32-33 1.4457425724035957 1104 34-35 1.5441717899993144 1104 36-37 2.0119685604888886 1104 38-39 2.0006841316436983 1104 40-41 1.3935873634096865 1104 42-43 1.2742090239933397 1104 44-45 1.2437210045804292 1104 46-47 1.0151409503302062 1104 48-49 0.9706773250255907 1104 50-51 1.1034188479747868 1104 52-53 1.303252147654927 1104 54-55 1.1133792334126085 1104 56-57 1.0659469855761792 1104 58-59 1.4283389350226514 1104 60-61 1.2988720747265106 1104 62-63 1.213714645990656 1104 64-65 1.1994353550722963 1104 66-67 1.0065383158696193 1104 68-69 1.2763822413170445 1104 70-71 1.3445859673861342 1104 72-73 1.6036969246999178 1104 74-75 1.4769121024697363 1104 76-77 1.409661090306468 1104 78-79 1.2920974359468111 1104 80-81 1.1803576212518472 1104 82-83 1.3968128180500372 1104 84-85 1.3709217278265022 1104 86-87 1.4422153415020738 1104 88-89 1.542575858827398 1104 90-91 1.4091320273301733 1104 92-93 1.2793882438133686 1104 94-95 1.3389994027044736 1104 96-97 1.2874605893256224 1104 98-99 1.4020975879112463 1104 100-101 1.43976685241363 1104 102-103 1.485979188227759 1104 104-105 1.589334971347217 1104 106-107 1.5883270304215422 1104 108-109 1.489425052682801 1104 110-111 1.209711631533807 1104 112-113 1.0356273266206166 1104 114-115 1.5797406707716988 1104 116-117 1.582409636354722 1104 118-119 1.6814337251786426 1104 120-121 1.7745469116325445 1104 122-123 1.6691636577380429 1104 124-125 1.6885623439383721 1105 1 0.5533397091363739 1105 2 0.45926021206691203 1105 3 0.7042944553368677 1105 4 0.30940140999032195 1105 5 0.20609635418745498 1105 6 -0.053100523327263005 1105 7 -0.1369668426052293 1105 8 -0.005509743493060881 1105 9 0.4336814562184266 1105 10-11 0.6218232229585041 1105 12-13 0.3038249027465234 1105 14-15 0.6706751435909339 1105 16-17 0.33361732878759653 1105 18-19 0.5745886964920217 1105 20-21 0.08860983295710056 1105 22-23 0.3144948139402821 1105 24-25 0.29114406064025644 1105 26-27 0.2778976782379523 1105 28-29 0.3304224673650431 1105 30-31 0.2775541618336632 1105 32-33 0.6811226893626596 1105 34-35 0.48130629292328564 1105 36-37 0.6274656365122837 1105 38-39 0.740450213515043 1105 40-41 0.7283826850471087 1105 42-43 0.5592967432915827 1105 44-45 0.8826098934693185 1105 46-47 0.8850239912658822 1105 48-49 0.7294492548501523 1105 50-51 0.8387989649338508 1105 52-53 1.0035445453157443 1105 54-55 0.9247827421845365 1105 56-57 1.12296452943583 1105 58-59 1.2397424437945794 1105 60-61 1.0459480981183198 1105 62-63 1.1961707863415327 1105 64-65 1.1921254135518282 1105 66-67 1.4758365614836535 1105 68-69 1.5219962764047636 1105 70-71 1.3548198855147895 1105 72-73 1.369778796044951 1105 74-75 1.2225261375574554 1105 76-77 1.2693102131134815 1105 78-79 1.322799190332777 1105 80-81 1.5312348142343026 1105 82-83 1.4231286075237186 1105 84-85 1.4191673418615878 1105 86-87 1.1819814233734185 1105 88-89 1.1653828763712575 1105 90-91 1.365272378207365 1105 92-93 1.270616313988807 1105 94-95 1.2702859524120775 1105 96-97 1.451203279384103 1105 98-99 1.4357233189053993 1105 100-101 1.3067259167411116 1105 102-103 1.0634645683447168 1105 104-105 1.2369957900606643 1105 106-107 1.4348182584917204 1105 108-109 1.3212963977120395 1105 110-111 1.344214555510412 1105 112-113 1.2125279114159362 1105 114-115 1.3063488579062046 1105 116-117 1.2096026188108588 1105 118-119 1.2355272924301026 1105 120-121 1.5026170870711404 1105 122-123 1.4235496226503237 1105 124-125 1.5072757942307646 1106 1 0.26600552000500954 1106 2 -0.15944610657522063 1106 3 0.5824858688299486 1106 4 0.7894149601258214 1106 5 0.876560624619632 1106 6 0.4612340937031192 1106 7 0.3367708552980666 1106 8 0.21444817977038255 1106 9 0.6330289102192843 1106 10-11 0.5517458445531425 1106 12-13 0.4692346160541838 1106 14-15 0.2318647028549492 1106 16-17 0.2922537572571464 1106 18-19 0.6790852019833942 1106 20-21 0.4523685692286783 1106 22-23 0.6296246099750817 1106 24-25 -3.212038438289998 1106 26-27 -0.35448357978514977 1106 28-29 -0.11044723738463702 1106 30-31 0.3160383295700768 1106 32-33 -4.516771690896931 1106 34-35 -0.9285496477413844 1106 36-37 0.10559821389068702 1106 38-39 0.7343526525394317 1106 40-41 0.1928581521701993 1106 42-43 0.4322909666548682 1106 44-45 0.43485814621500296 1106 46-47 0.5142316649215637 1106 48-49 0.40264138150826057 1106 50-51 0.8404125008060532 1106 52-53 1.0199027681084978 1106 54-55 1.0711687784133197 1106 56-57 0.8215185873448263 1106 58-59 0.8558635392160632 1106 60-61 0.8376860812876288 1106 62-63 0.7308840940009276 1106 64-65 0.6530974574827937 1106 66-67 0.36574171053513993 1106 68-69 0.5090255874873506 1106 70-71 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84-85 -4.575163509246998 2216 86-87 -4.183315812522071 2216 88-89 -4.079715781544515 2216 90-91 -3.922518441768233 2216 92-93 -3.9906378405822736 2216 94-95 -3.8537516596035744 2216 96-97 -3.8095460159164993 2216 98-99 -3.702408281077769 2216 100-101 -3.8947865574900256 2216 102-103 -3.67960413458767 2216 104-105 -3.935203292778329 2216 106-107 -3.867003442042588 2216 108-109 -3.6820062233490063 2216 110-111 -3.909361530988811 2216 112-113 -4.244758890721304 2216 114-115 -3.8639149310626237 2216 116-117 -3.6557892781978474 2216 118-119 -3.7939017958781953 2216 120-121 -3.7605092539612954 2216 122-123 -3.5380284832859807 2216 124-125 -3.57597339589082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15781.0 3 1842.0 4 1470.0 5 1416.0 6 2278.0 7 2571.0 8 2272.0 9 2326.0 10 2879.0 11 3309.0 12 3171.0 13 2961.0 14 2995.0 15 2988.0 16 2706.0 17 2425.0 18 2005.0 19 1627.0 20 1367.0 21 1246.0 22 1288.0 23 1973.0 24 3291.0 25 5271.0 26 5297.0 27 3510.0 28 3691.0 29 4241.0 30 4863.0 31 5649.0 32 7124.0 33 9035.0 34 11991.0 35 16358.0 36 23394.0 37 32658.0 38 12230.0 39 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.32675265503665 18.240940736084394 9.028306211864127 18.404000397014826 2 18.019746761257025 25.330491211320595 36.77444358844687 19.875318438975512 3 17.891105019378013 38.47953492768693 26.173551375366294 17.45580867756877 4 9.057137050463421 21.867954759215234 31.90864877894309 37.166259411378256 5 9.625291854540642 52.35941354961291 27.15027082210816 10.86502377373829 6 24.16272013158268 39.280549017383656 21.513578916522512 15.043151934511151 7 27.514935531440237 42.2250160698756 16.482493288463722 13.77755511022044 8 26.56681287102507 39.33338374862934 17.30300223087685 16.79680114946875 9 28.523353531152225 22.265968408216512 15.32135328537533 33.88932477525594 10-11 21.698400807276837 35.975573731363035 23.474362579788302 18.851662881571826 12-13 24.110770016093696 35.099858914278016 22.952543678674314 17.83682739095397 14-15 22.225373215454677 34.26137961512193 22.607746244213004 20.905500925210386 16-17 20.53249012156618 35.01952054147052 24.23997504395667 20.208014293006634 18-19 20.432331004754836 32.815232495486214 27.058617788575155 19.693818711183795 20-21 24.2600697628253 29.786741282010077 26.015483944151928 19.937705011012696 22-23 23.441680248470124 30.9667484334602 24.77581153739707 20.8157597806726 24-25 21.975658749852297 30.643270707786836 26.76214108472173 20.618929457639133 26-27 21.966102015351456 30.333544447385336 26.80738836162892 20.892965175634288 28-29 21.154473312316558 30.03681874683921 27.6095700383312 21.199137902513034 30-31 22.11671846013872 30.76626767969184 27.009251928962204 20.10776193120724 32-33 20.346116761825538 31.388484516202215 26.605569629825688 21.659829092146555 34-35 21.153055388818256 30.97501146154828 27.58097524778213 20.290957901851336 36-37 21.284771373272804 30.571631805689144 26.806835288084564 21.336761532953492 38-39 21.631789769706135 30.90546017416786 26.64484645106403 20.817903605061975 40-41 21.265543986350124 31.54548934903132 26.965123808353457 20.223842856265094 42-43 20.004111987862547 32.270833481034046 27.31494282209981 20.4101117090036 44-45 20.504688622528075 32.20237645101523 26.764141490528225 20.52879343592846 46-47 20.60418005822967 32.62373804212198 25.821217907513137 20.95086399213521 48-49 20.05969415606973 32.33338926214147 26.419508598463455 21.187407983325336 50-51 20.709815858131357 32.334952924717356 26.640781563126254 20.314449654025033 52-53 20.52496378378506 32.97341623478978 25.75687753602125 20.744742445403908 54-55 20.082145422921315 32.68872650962302 26.74736264982796 20.48176541762771 56-57 20.619777290429916 33.00468862252808 25.405291715506483 20.970242371535523 58-59 20.14963819318735 32.39813401267624 25.812352004234867 21.63987578990155 60-61 19.878861782853956 32.65958175884373 26.216994798571676 21.24456165973064 62-63 20.569440768329113 32.08185238401331 25.872263394713958 21.476443452943624 64-65 19.818410405717092 32.56040382652097 26.062975006617005 21.55821076114493 66-67 19.449046098729315 32.93222137409992 25.59806407550862 22.020668451662146 68-69 20.192457663567744 32.75915851762226 25.53703344849914 21.511350370310854 70-71 19.676997027087065 32.63327299281113 25.70151765078435 21.98821232931746 72-73 19.698505762609326 32.69645688736385 25.38549339609934 22.219543953927484 74-75 19.534357224387165 33.234709575878455 25.552927164149473 21.6780060355849 76-77 19.69599935721257 32.954435351418034 26.23943359084217 21.110131700527226 78-79 20.482420278004906 31.911720917482363 26.24386163088019 21.361997173632545 80-81 19.668303565098615 33.02619825218949 26.213849200535023 21.09164898217687 82-83 19.766139361656876 32.64501675497095 25.404931491310574 22.183912392061593 84-85 19.858539836184118 32.33118598726717 25.796747314241962 22.01352686230675 86-87 20.106674102817387 32.22437009344027 25.745466232471088 21.92348957127125 88-89 21.051288292524998 31.093896591572395 26.460628088392724 21.394187027509883 90-91 20.087957481703096 32.593186012822606 25.57572921762269 21.74312728785161 92-93 19.855704015994025 32.40775313239972 25.852282126297975 21.884260725308277 94-95 19.580110549462496 32.57262636503064 26.053091154861413 21.794171930645454 96-97 19.887559995179117 33.080945932852664 25.335394024468343 21.696100047499876 98-99 19.580819502834633 32.88763798005005 24.94003436060677 22.591508156508546 100-101 19.271052420136215 34.13594921991313 25.21327731013002 21.379721049820635 102-103 19.057837099495693 34.81049452445209 25.198627450053646 20.933040925998572 104-105 18.17331074135254 35.288390415895684 25.47671206331426 21.061586779437516 106-107 17.714145273998664 36.538984164344846 24.77721140280604 20.969659158850458 108-109 17.531164703241128 37.24521747350262 23.621368056622277 21.602249766633978 110-111 17.4625193783794 37.37994857639808 24.506087646992096 20.651444398230424 112-113 18.25582879208239 36.88244107401963 23.98843930635838 20.873290827539595 114-115 17.588743657791316 37.68648515799101 23.812911047199975 20.9118601370177 116-117 17.882808899812126 37.01811392988385 23.931183608843096 21.16789356146093 118-119 17.624483043837884 37.48150773957226 24.098782937492615 20.79522627909725 120-121 17.790449812597778 36.91091186660176 23.608189926126187 21.69044839467428 122-123 18.141300135174735 36.39863312820803 23.763340233861744 21.696726502755485 124-125 17.63596576219757 36.620127706435895 23.615528951360957 22.128377580005576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 117.0 1 176.5 2 251.5 3 258.0 4 238.0 5 217.5 6 195.0 7 182.0 8 181.5 9 196.5 10 191.0 11 155.5 12 147.5 13 167.0 14 195.0 15 217.0 16 255.5 17 310.0 18 368.0 19 460.0 20 542.0 21 633.0 22 833.5 23 1059.5 24 1269.5 25 1585.0 26 2124.0 27 2675.5 28 3059.5 29 3463.5 30 3957.5 31 4324.0 32 4898.5 33 5717.0 34 6733.0 35 7521.5 36 8212.0 37 9016.0 38 8992.0 39 8552.0 40 8045.0 41 7278.5 42 6744.0 43 6639.5 44 6733.0 45 6564.5 46 6204.5 47 6066.0 48 6416.0 49 6279.5 50 5806.5 51 5456.5 52 4471.0 53 3813.0 54 3503.5 55 3186.5 56 3016.0 57 2923.0 58 3141.0 59 3201.0 60 3393.5 61 3495.0 62 2829.5 63 1665.0 64 831.5 65 678.5 66 493.5 67 365.0 68 352.0 69 351.5 70 351.0 71 328.5 72 282.0 73 288.0 74 247.5 75 182.5 76 129.0 77 70.0 78 47.5 79 38.5 80 23.5 81 16.0 82 13.0 83 5.5 84 3.0 85 3.5 86 4.5 87 3.0 88 3.0 89 2.5 90 0.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.726344645051517E-4 2 4.726344645051517E-4 3 0.0 4 4.726344645051517E-4 5 9.452689290103034E-4 6 9.452689290103034E-4 7 0.001890537858020607 8 0.001890537858020607 9 0.0028358067870309102 10-11 0.0021268550902731826 12-13 0.003072124019283486 14-15 0.003072124019283486 16-17 0.003781075716041214 18-19 0.0028358067870309102 20-21 0.0028358067870309102 22-23 0.09759901692031384 24-25 0.0023631723225257585 26-27 0.001890537858020607 28-29 0.0014179033935154551 30-31 0.0011815861612628792 32-33 0.001890537858020607 34-35 0.0014179033935154551 36-37 7.089516967577276E-4 38-39 0.0011815861612628792 40-41 0.0014179033935154551 42-43 0.001654220625768031 44-45 0.001890537858020607 46-47 0.001890537858020607 48-49 7.089516967577276E-4 50-51 0.001890537858020607 52-53 0.001654220625768031 54-55 0.001890537858020607 56-57 0.001890537858020607 58-59 0.0014179033935154551 60-61 0.001654220625768031 62-63 0.001890537858020607 64-65 0.001890537858020607 66-67 7.089516967577276E-4 68-69 4.726344645051517E-4 70-71 0.0014179033935154551 72-73 2.3631723225257586E-4 74-75 7.089516967577276E-4 76-77 7.089516967577276E-4 78-79 4.726344645051517E-4 80-81 4.726344645051517E-4 82-83 4.726344645051517E-4 84-85 4.726344645051517E-4 86-87 4.726344645051517E-4 88-89 7.089516967577276E-4 90-91 2.3631723225257586E-4 92-93 4.726344645051517E-4 94-95 2.3631723225257586E-4 96-97 2.3631723225257586E-4 98-99 2.3631723225257586E-4 100-101 4.726344645051517E-4 102-103 0.0014179033935154551 104-105 2.3631723225257586E-4 106-107 2.3631723225257586E-4 108-109 0.0011815861612628792 110-111 0.001890537858020607 112-113 4.726344645051517E-4 114-115 7.089516967577276E-4 116-117 0.0011815861612628792 118-119 0.0023631723225257585 120-121 0.0014179033935154551 122-123 9.452689290103034E-4 124-125 4.726344645051517E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 211580.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.57174350293708 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3595661489502 35.146302869706176 2 10.672765100667144 10.36789617873406 3 5.9438442326725935 8.661086324723552 4 3.6633242687584078 7.1173618700087165 5 2.3069049960003314 5.602519887568609 6 1.3843413410107335 4.034392352165119 7 0.9435509959168322 3.208094186892925 8 0.560733163291593 2.1788629904791827 9 0.3948121201960151 1.7259041730610447 >10 1.6242270767201243 13.336797056853571 >50 0.0959543380708238 3.29451075052941 >100 0.04597812032560307 3.9970095798863294 >500 0.003998097419617658 1.329261779391324 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 996 0.4707439266471311 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 606 0.28641648549012194 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 596 0.28169014084507044 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 540 0.2552226108327819 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 431 0.2037054542017204 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 402 0.18999905473107098 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 375 0.1772379241894319 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 306 0.14462614613857644 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 293 0.13848189810000944 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 288 0.13611872577748368 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 286 0.1351734568484734 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 248 0.11721334719727763 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.10965119576519519 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 230 0.10870592683618491 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 224 0.10587012004915398 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 213 0.10067114093959732 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.726344645051517E-4 0.0 18-19 0.0 0.0 0.0 7.089516967577276E-4 0.0 20-21 0.0 0.0 0.0 9.452689290103034E-4 0.0 22-23 0.0 0.0 0.0 0.001654220625768031 0.0 24-25 0.0 0.0 0.0 0.001890537858020607 0.0 26-27 0.0 0.0 0.0 0.0028358067870309102 0.0 28-29 0.0 0.0 0.0 0.00401739294829379 0.0 30-31 0.0 0.0 0.0 0.010161640986860761 0.0 32-33 0.0 0.0 0.0 0.03402968144437092 0.0 34-35 0.0 0.0 0.0 0.06735041119198412 0.0 36-37 0.0 0.0 0.0 0.11792229889403535 0.0 38-39 0.0 0.0 0.0 0.18172795160223082 0.0 40-41 0.0 0.0 0.0 0.2616031761036015 0.0 42-43 0.0 0.0 0.0 0.3379336421211835 0.0 44-45 0.0 0.0 0.0 0.43978636922204367 0.0 46-47 0.0 0.0 0.0 0.5534549579355326 0.0 48-49 0.0 0.0 0.0 0.6595613952169392 0.0 50-51 0.0 0.0 0.0 0.7765384251819643 0.0 52-53 0.0 0.0 0.0 0.8970602136307779 0.0 54-55 0.0 0.0 0.0 1.0466490216466584 0.0 56-57 0.0 0.0 0.0 1.2269590698553738 0.0 58-59 0.0 0.0 0.0 1.4540599300500991 0.0 60-61 0.0 0.0 0.0 1.6769070800642782 0.0 62-63 0.0 0.0 0.0 1.881085168730504 0.0 64-65 0.0 0.0 0.0 2.1183476699120902 0.0 66-67 0.0 0.0 0.0 2.404527838169959 0.0 68-69 0.0 0.0 0.0 2.7379714528783436 0.0 70-71 0.0 0.0 0.0 3.0886662255411665 0.0 72-73 0.0 0.0 0.0 3.4067492201531335 0.0 74-75 0.0 0.0 0.0 3.760988751299745 0.0 76-77 0.0 0.0 0.0 4.175725493903015 0.0 78-79 0.0 0.0 0.0 4.6835712260138 0.0 80-81 0.0 0.0 0.0 5.178892144815199 0.0 82-83 0.0 0.0 0.0 5.652235561017109 0.0 84-85 0.0 0.0 0.0 6.149447017676529 0.0 86-87 0.0 0.0 0.0 6.7742697797523395 0.0 88-89 0.0 0.0 0.0 7.4487191606011915 0.0 90-91 0.0 0.0 0.0 8.087957273844408 0.0 92-93 0.0 0.0 0.0 8.733339635126192 0.0 94-95 0.0 0.0 0.0 9.39171944418187 0.0 96-97 0.0 0.0 0.0 10.133755553454957 0.0 98-99 0.0 0.0 0.0 10.939597315436242 0.0 100-101 0.0 0.0 0.0 11.819642688344835 0.0 102-103 0.0 0.0 0.0 12.655496738822196 0.0 104-105 0.0 0.0 0.0 13.450231590887608 0.0 106-107 0.0 0.0 0.0 14.294829378958314 0.0 108-109 0.0 0.0 0.0 15.18527271008602 0.0 110-111 0.0 0.0 0.0 16.178514037243595 0.0 112-113 0.0 0.0 0.0 17.13678041402779 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGGT 25 1.7230428E-5 95.19338 7 CGTCACT 55 2.0008883E-11 86.53944 1 TCCCGCT 25 0.0020550878 71.395035 8 CTAGGGC 25 0.0020550878 71.395035 3 ATCCCGT 20 1.760977E-4 59.566257 20-21 TCCCGTT 20 1.760977E-4 59.566257 20-21 GCCGGTC 40 1.7692696E-4 59.49587 8 CGGCCGT 15 0.0042358707 59.49586 52-53 CGAATGC 15 0.0042358707 59.49586 42-43 CATTCCG 55 1.3075765E-5 54.08715 9 GTCACTA 100 6.91216E-11 53.546276 2 GTACATA 135 0.0 52.885216 1 TCTTGCG 45 3.1660957E-4 52.88521 2 CTTGCGC 45 3.1660957E-4 52.88521 3 ATGGGCG 70 9.109863E-7 50.996456 5 ACATGGA 420 0.0 48.16332 3 GTACATG 4225 0.0 47.73751 1 AACCGCG 25 5.3305866E-4 47.59669 102-103 TCTAGCG 25 5.3305866E-4 47.59669 28-29 GTTCCGA 25 5.3305866E-4 47.59669 80-81 >>END_MODULE