##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936224_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 211580 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46014273560828 31.0 31.0 34.0 30.0 34.0 2 31.146511957651953 31.0 31.0 34.0 28.0 34.0 3 31.42151904716892 31.0 31.0 34.0 28.0 34.0 4 35.37389167218073 37.0 35.0 37.0 33.0 37.0 5 35.12078173740429 37.0 35.0 37.0 33.0 37.0 6 35.39041969940448 37.0 35.0 37.0 33.0 37.0 7 35.191185367236976 37.0 35.0 37.0 32.0 37.0 8 35.30597409963134 37.0 35.0 37.0 32.0 37.0 9 36.84828433689385 39.0 37.0 39.0 32.0 39.0 10-11 36.77067066830513 39.0 37.0 39.0 32.0 39.0 12-13 36.74180688155781 39.0 37.0 39.0 32.0 39.0 14-15 37.55417336232158 39.0 36.0 41.0 32.0 41.0 16-17 37.5321131486908 39.0 36.5 41.0 32.0 41.0 18-19 37.948487569713585 40.0 37.0 41.0 32.5 41.0 20-21 38.051514793458736 40.0 37.0 41.0 33.0 41.0 22-23 37.8814939975423 40.0 37.0 41.0 32.5 41.0 24-25 37.75032848095283 40.0 36.5 41.0 32.0 41.0 26-27 37.42285187635882 40.0 36.0 41.0 32.0 41.0 28-29 37.1537763493714 39.5 35.0 41.0 32.0 41.0 30-31 36.56539843085358 38.5 35.0 40.5 31.0 41.0 32-33 35.77322998393043 38.0 35.0 40.0 30.0 41.0 34-35 35.15635929671991 38.0 35.0 40.0 29.0 41.0 36-37 34.520729747613196 38.0 34.0 40.0 24.5 41.0 38-39 34.16459967860857 37.5 34.0 40.0 23.5 41.0 40-41 33.734703185556285 38.0 33.5 40.0 18.0 41.0 42-43 33.200099253237546 38.0 33.0 40.0 5.5 41.0 44-45 32.29736269968806 38.0 33.0 40.0 2.0 41.0 46-47 30.884847339067967 38.0 30.5 40.0 2.0 41.0 48-49 29.69306645240571 37.0 25.0 40.0 2.0 41.0 50-51 29.188637867473297 37.0 23.0 40.0 2.0 41.0 52-53 28.966301162680786 37.0 21.5 40.0 2.0 41.0 54-55 28.74733434162019 37.0 20.0 40.0 2.0 41.0 56-57 28.581727951602232 36.0 19.0 40.0 2.0 41.0 58-59 28.39987002552226 36.0 18.0 40.0 2.0 41.0 60-61 28.19765336988373 36.0 17.0 40.0 2.0 41.0 62-63 27.995122412326307 35.0 14.5 40.0 2.0 41.0 64-65 27.767681255317136 35.0 13.0 39.0 2.0 41.0 66-67 27.53607146233103 35.0 10.5 39.0 2.0 41.0 68-69 27.293484733906794 35.0 8.0 39.0 2.0 41.0 70-71 27.0156418376028 35.0 7.0 38.5 2.0 40.5 72-73 26.780692882124967 35.0 6.5 37.5 2.0 40.0 74-75 26.60865393704509 35.0 2.0 37.0 2.0 39.5 76-77 26.316010492485113 34.0 2.0 36.5 2.0 39.0 78-79 26.005312411381038 34.0 2.0 36.0 2.0 39.0 80-81 25.761343227148124 34.0 2.0 36.0 2.0 38.5 82-83 25.548239436619717 34.0 2.0 35.5 2.0 37.0 84-85 25.316591832876455 34.0 2.0 35.0 2.0 37.0 86-87 25.09806456186785 34.0 2.0 35.0 2.0 36.5 88-89 24.905376217033748 34.0 2.0 35.0 2.0 36.0 90-91 24.727464788732394 34.0 2.0 35.0 2.0 36.0 92-93 24.614065601663675 34.0 2.0 35.0 2.0 36.0 94-95 24.524385575196142 34.0 2.0 35.0 2.0 35.0 96-97 24.445450893279137 34.0 2.0 35.0 2.0 35.0 98-99 24.345311466112108 33.0 2.0 35.0 2.0 35.0 100-101 24.26627280461291 33.0 2.0 35.0 2.0 35.0 102-103 23.392924662066356 31.5 2.0 34.5 2.0 35.0 104-105 23.97964127044144 33.0 2.0 35.0 2.0 35.0 106-107 24.11641695812459 33.0 2.0 35.0 2.0 35.0 108-109 24.07533793364212 33.0 2.0 35.0 2.0 35.0 110-111 24.03296152755459 33.0 2.0 35.0 2.0 35.0 112-113 23.937638245580867 33.0 2.0 35.0 2.0 35.0 114-115 23.860922582474714 33.0 2.0 35.0 2.0 35.0 116-117 23.756623972020037 33.0 2.0 35.0 2.0 35.0 118-119 23.653807070611588 33.0 2.0 35.0 2.0 35.0 120-121 23.439013611872575 33.0 2.0 35.0 2.0 35.0 122-123 23.189828906323847 33.0 2.0 35.0 2.0 35.0 124-125 21.441655638529163 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17464754227335888 1101 2 0.6192100891372725 1101 3 0.38948175215274716 1101 4 0.11093312924442245 1101 5 0.6198717075983424 1101 6 0.3107728712943256 1101 7 0.42339500259895857 1101 8 0.49915928491926564 1101 9 0.2281573943017534 1101 10-11 0.5147395674685029 1101 12-13 0.5892054011507781 1101 14-15 0.5876427767375816 1101 16-17 0.8889971939510417 1101 18-19 0.7488298450580899 1101 20-21 0.7443160818252892 1101 22-23 0.5293679158413696 1101 24-25 0.7543973491191878 1101 26-27 0.6997948174882254 1101 28-29 0.5826967253560866 1101 30-31 0.2934092421908687 1101 32-33 0.3619583504078747 1101 34-35 0.8588964824014127 1101 36-37 0.6719088244132365 1101 38-39 0.971604982965637 1101 40-41 0.5728371741220997 1101 42-43 0.4810973837397938 1101 44-45 0.45195531598183436 1101 46-47 0.5228431031348855 1101 48-49 0.6820308150855823 1101 50-51 0.49925029899857165 1101 52-53 0.40764264021632357 1101 54-55 0.1995818825642175 1101 56-57 0.21956744143514229 1101 58-59 0.4261750691827828 1101 60-61 0.27209819997822393 1101 62-63 0.4424212847819611 1101 64-65 0.47511154312538295 1101 66-67 0.5214520794873785 1101 68-69 0.4095300638876296 1101 70-71 0.6603367747303288 1101 72-73 0.6340216625848853 1101 74-75 0.45224348997566466 1101 76-77 0.6301506044988621 1101 78-79 0.8300782123598438 1101 80-81 0.8008076315484765 1101 82-83 0.4193231547347587 1101 84-85 0.35112413073181514 1101 86-87 0.12812254207961615 1101 88-89 0.3025811049687377 1101 90-91 0.500285507571931 1101 92-93 0.3292482481049177 1101 94-95 0.4076012526065931 1101 96-97 0.3561513145558024 1101 98-99 0.441635249518459 1101 100-101 0.326862387309621 1101 102-103 0.28854651967534295 1101 104-105 0.25835810462757536 1101 106-107 0.4227768610703997 1101 108-109 0.4915369988640421 1101 110-111 0.5032539015929274 1101 112-113 0.07924628299045366 1101 114-115 0.0733189638632652 1101 116-117 0.03822650182500098 1101 118-119 0.0946138377408623 1101 120-121 -0.08303072300813596 1101 122-123 0.19289033435063274 1101 124-125 0.4846945051519569 1102 1 0.301162693788509 1102 2 0.7448729679251507 1102 3 0.42660296427396105 1102 4 0.5087550989413927 1102 5 0.4498906469922801 1102 6 0.3386137803852378 1102 7 0.5020881844171399 1102 8 0.5940456485556354 1102 9 0.5169831518775112 1102 10-11 0.550819112923044 1102 12-13 0.5081921435750232 1102 14-15 0.6998113373436397 1102 16-17 0.7515445424358944 1102 18-19 0.8751556026338534 1102 20-21 1.0108407030374096 1102 22-23 0.4222182946292534 1102 24-25 0.8400034097252416 1102 26-27 0.7743213326397438 1102 28-29 0.6386152859621461 1102 30-31 0.3695929543120826 1102 32-33 0.6331231231351495 1102 34-35 0.6660934520983872 1102 36-37 0.16357549107990366 1102 38-39 -0.02527001703436582 1102 40-41 0.04447543169785462 1102 42-43 0.534506474648893 1102 44-45 0.9291806947697125 1102 46-47 0.9071707546500356 1102 48-49 0.6691993756916403 1102 50-51 0.39849272324099516 1102 52-53 0.5628983220345063 1102 54-55 0.6438527158975482 1102 56-57 0.7084405474957443 1102 58-59 0.5089875691827821 1102 60-61 0.5473822908873132 1102 62-63 0.5915216635698393 1102 64-65 0.48950548251932346 1102 66-67 0.06236117039646771 1102 68-69 0.03836529116035692 1102 70-71 0.3663026838212389 1102 72-73 0.27440992016064314 1102 74-75 0.25659955058172557 1102 76-77 0.45141007419583445 1102 78-79 0.2580611669052999 1102 80-81 0.394936419427264 1102 82-83 0.370554215340821 1102 84-85 0.5744195852772691 1102 86-87 0.7355089057159816 1102 88-89 0.5541909534535847 1102 90-91 0.46993512878405497 1102 92-93 0.42380317234734477 1102 94-95 0.32696678290962566 1102 96-97 0.3107441175861041 1102 98-99 0.38159737073058153 1102 100-101 0.2848642812490141 1102 102-103 0.3411506863420115 1102 104-105 0.3662179531124252 1102 106-107 0.4127389822825229 1102 108-109 0.5263381352276753 1102 110-111 0.44624632583535373 1102 112-113 0.2636212829904565 1102 114-115 0.3096352517420513 1102 116-117 0.6390787745522708 1102 118-119 0.3857123225893524 1102 120-121 0.697461701234289 1102 122-123 0.8026441222294238 1102 124-125 0.6615884445458953 1103 1 0.24753273864797976 1103 2 0.7059642432158206 1103 3 0.2730920240560728 1103 4 0.24026658845892968 1103 5 0.4089861335802141 1103 6 0.4240848048290715 1103 7 0.33688631679835623 1103 8 0.2742159314449495 1103 9 0.4222472734558309 1103 10-11 0.3988690991905557 1103 12-13 0.3216591398214774 1103 14-15 0.40446207432066217 1103 16-17 0.4772288782410783 1103 18-19 0.685404618471992 1103 20-21 0.7477856815231689 1103 22-23 0.6885965789833719 1103 24-25 0.5405755968533228 1103 26-27 0.7041849232284108 1103 28-29 0.4156225184074458 1103 30-31 0.07514545820295382 1103 32-33 -0.16271593206946022 1103 34-35 0.006366270921049022 1103 36-37 0.339658069126223 1103 38-39 0.2235215237511312 1103 40-41 0.17511719678070392 1103 42-43 0.4052288036793783 1103 44-45 0.5189728764047885 1103 46-47 -0.2781812548711571 1103 48-49 -0.37720728914402457 1103 50-51 -0.5336046858956855 1103 52-53 -0.5637839081220406 1103 54-55 -0.44763771713367007 1103 56-57 -0.43115480176727417 1103 58-59 -0.24267406223715682 1103 60-61 -0.13431229851120463 1103 62-63 -0.37971334512740285 1103 64-65 -0.5894654961495398 1103 66-67 -0.5193334190020487 1103 68-69 -0.6845267714598009 1103 70-71 -0.6421839956624176 1103 72-73 -0.8387549612821843 1103 74-75 -0.8574808302660273 1103 76-77 -0.8542385571325575 1103 78-79 -0.923674619966441 1103 80-81 -0.8498910089349074 1103 82-83 -0.9279672531202259 1103 84-85 -0.9038418813527755 1103 86-87 -0.8332634095820168 1103 88-89 -0.8488256170856445 1103 90-91 -1.0999174758117505 1103 92-93 -1.0519498259132085 1103 94-95 -1.181432924130565 1103 96-97 -1.2457605056858938 1103 98-99 -1.0977718501794271 1103 100-101 -0.9205554752281415 1103 102-103 -0.9267244395391607 1103 104-105 -1.089215535855807 1103 106-107 -1.0447269153646452 1103 108-109 -1.0280013319516712 1103 110-111 -0.9144009322439288 1103 112-113 -0.9889466928403579 1103 114-115 -0.9202374938406628 1103 116-117 -0.9797210057278711 1103 118-119 -1.000797793679073 1103 120-121 -1.0201341973283746 1103 122-123 -0.9915790765254968 1103 124-125 -0.6057511141833913 1104 1 0.41806859490493764 1104 2 0.47134824703200806 1104 3 0.37471567028140385 1104 4 0.24432128713916512 1104 5 0.667806649118809 1104 6 0.31373339761011465 1104 7 0.39336941780364043 1104 8 0.4789472966151749 1104 9 0.2558986223719302 1104 10-11 0.4132593043105999 1104 12-13 0.4660602988115983 1104 14-15 0.7856965048077598 1104 16-17 0.7132936120797027 1104 18-19 0.6757669795610184 1104 20-21 0.5726695174978005 1104 22-23 0.49255322285891623 1104 24-25 0.37559617952854296 1104 26-27 0.37203531456424344 1104 28-29 0.6408747224321019 1104 30-31 0.4951910404364881 1104 32-33 0.4486999439751287 1104 34-35 0.5873321549160337 1104 36-37 0.4849205203196689 1104 38-39 0.7852562987551082 1104 40-41 1.0602914869875946 1104 42-43 0.9453517697047147 1104 44-45 0.9895741025315345 1104 46-47 1.156532796117336 1104 48-49 0.21064009871130907 1104 50-51 0.30846082531435925 1104 52-53 0.13839008173679446 1104 54-55 0.19531470420164254 1104 56-57 0.07694134494391491 1104 58-59 0.3171747036857049 1104 60-61 0.4225550713232522 1104 62-63 0.220920919284886 1104 64-65 0.4816904904938042 1104 66-67 0.49952225492597435 1104 68-69 0.5608001662268123 1104 70-71 0.49142230104611784 1104 72-73 0.4630329929942434 1104 74-75 0.33517477652537053 1104 76-77 0.3825902974813218 1104 78-79 0.4631648351668609 1104 80-81 0.5178763449987649 1104 82-83 0.48684873953007823 1104 84-85 0.2844940137727505 1104 86-87 0.6443871619626549 1104 88-89 0.5475189704658092 1104 90-91 0.6118809023087763 1104 92-93 0.7975139644791902 1104 94-95 0.6826286648872966 1104 96-97 0.6062518262517074 1104 98-99 0.5524905126763535 1104 100-101 0.49560324988271987 1104 102-103 0.3616185225993185 1104 104-105 0.7902477245106176 1104 106-107 0.9052695511288817 1104 108-109 0.9098575397997131 1104 110-111 0.8307758314174905 1104 112-113 0.8770715753881149 1104 114-115 0.847487458015312 1104 116-117 1.08243337317003 1104 118-119 1.064898925460163 1104 120-121 1.0489502711439123 1104 122-123 0.8387419425377658 1104 124-125 0.8066974291285653 1105 1 0.3654370159575677 1105 2 0.781289767499846 1105 3 0.19635309718198712 1105 4 0.15367801228536138 1105 5 0.5566955380076948 1105 6 0.36636497655748457 1105 7 0.5191004119556837 1105 8 0.3649122088958734 1105 9 0.26759452880467904 1105 10-11 0.14717743296557018 1105 12-13 0.2906217023203652 1105 14-15 0.262304691942262 1105 16-17 0.22791349512063874 1105 18-19 0.22255060529201387 1105 20-21 0.04050577480774109 1105 22-23 0.2674070240285076 1105 24-25 0.2469412087683054 1105 26-27 0.20390665959348553 1105 28-29 -0.07842352318192525 1105 30-31 0.09460624511484639 1105 32-33 -0.11416555310088938 1105 34-35 -0.28108889771554857 1105 36-37 -0.810401117107233 1105 38-39 -0.5700068591396246 1105 40-41 -0.19848044283696709 1105 42-43 -0.4157593414063996 1105 44-45 -0.7428820378193386 1105 46-47 -0.49551398750839226 1105 48-49 -0.0583657492419043 1105 50-51 -0.07458011035815204 1105 52-53 -0.16277950890648185 1105 54-55 -0.0868490384884133 1105 56-57 -0.06194754394497792 1105 58-59 0.08471856333482819 1105 60-61 -0.08036890528493501 1105 62-63 -0.2366814198964029 1105 64-65 0.20537470102011923 1105 66-67 0.12525324907802116 1105 68-69 -0.015223225586055378 1105 70-71 -0.014425652170256598 1105 72-73 0.0010446889006772153 1105 74-75 0.1553502151218602 1105 76-77 0.07703474192576465 1105 78-79 0.1342174667458096 1105 80-81 0.15676523388765773 1105 82-83 0.03801833017335454 1105 84-85 0.027184072252282476 1105 86-87 0.22333453038370976 1105 88-89 0.053366923682187206 1105 90-91 0.11772885552515078 1105 92-93 0.04751396447919021 1105 94-95 0.053973694127062544 1105 96-97 -0.1569060684851351 1105 98-99 -0.029380832352885022 1105 100-101 -0.05556634076055289 1105 102-103 0.07506881499697826 1105 104-105 0.22738222743459247 1105 106-107 0.05731633475461351 1105 108-109 -0.07406058885525724 1105 110-111 -0.12097855454742046 1105 112-113 0.08467391456940021 1105 114-115 0.18666874456501859 1105 116-117 0.05173161878406418 1105 118-119 0.10437260967069051 1105 120-121 0.006552610325201158 1105 122-123 0.08143200101729775 1105 124-125 0.21459216597067154 1106 1 0.14111095690750375 1106 2 0.6282802110884909 1106 3 0.2089939472746991 1106 4 0.2305215438785737 1106 5 0.21842353686663074 1106 6 0.2880594566601786 1106 7 0.3263675635745713 1106 8 0.21516538248024375 1106 9 0.3209927601554128 1106 10-11 0.08463286015142302 1106 12-13 0.27643863285810255 1106 14-15 0.055058935274168164 1106 16-17 0.02165725492665871 1106 18-19 -0.33790795981995814 1106 20-21 -0.028359223052760285 1106 22-23 -0.0666305597683774 1106 24-25 0.20653149546064498 1106 26-27 0.3899625004150522 1106 28-29 0.13136288389267037 1106 30-31 0.4882644251176984 1106 32-33 0.5594812162615312 1106 34-35 0.6180428238648261 1106 36-37 0.7411161414864083 1106 38-39 0.5137543732095367 1106 40-41 0.4515338204635597 1106 42-43 -0.1557928601626415 1106 44-45 0.06807574281110362 1106 46-47 0.5785214567934212 1106 48-49 0.4854225833782664 1106 50-51 0.4321771282668614 1106 52-53 0.39327526216754904 1106 54-55 0.4733242606129977 1106 56-57 0.5160994536302645 1106 58-59 0.29282903259741744 1106 60-61 0.4918390536367596 1106 62-63 0.4298069555136692 1106 64-65 0.2601725187351356 1106 66-67 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2216 94-95 -2.2411060315660656 2216 96-97 -2.757394099113263 2216 98-99 -2.9640751821362166 2216 100-101 -2.7144707961436154 2216 102-103 -2.6816310882383263 2216 104-105 -2.666439557242928 2216 106-107 -3.1055954410878748 2216 108-109 -2.68484564502085 2216 110-111 -3.0538953789826095 2216 112-113 -3.2050837529807694 2216 114-115 -3.035437780741052 2216 116-117 -3.392281591700179 2216 118-119 -3.3656829248490645 2216 120-121 -3.530018133080077 2216 122-123 -3.365810205217713 2216 124-125 -3.3817623293804147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 22.0 4 56.0 5 131.0 6 341.0 7 650.0 8 1149.0 9 2353.0 10 4278.0 11 5603.0 12 6084.0 13 7948.0 14 11346.0 15 7988.0 16 2336.0 17 1434.0 18 1126.0 19 1136.0 20 1160.0 21 1051.0 22 1157.0 23 1202.0 24 1364.0 25 1659.0 26 1921.0 27 2347.0 28 2969.0 29 3741.0 30 4536.0 31 5584.0 32 7041.0 33 8817.0 34 11995.0 35 16643.0 36 25150.0 37 39539.0 38 19544.0 39 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.86383522122639 16.153427767398966 13.338378224986403 16.644358786388253 2 19.87106470869037 23.128004197013883 36.77822468203366 20.222706412262088 3 18.486624444654502 31.238774931468004 34.965497684091126 15.30910293978637 4 9.939975423007846 18.810379052840535 35.87248322147651 35.37716230267512 5 8.375824196615236 43.72238882214381 34.19008788277252 13.711699098468436 6 24.71925512808394 36.900463181775216 19.27450609698459 19.105775593156253 7 21.644295302013422 38.597220909348714 22.471878249361943 17.286605539275925 8 28.954532564514608 30.852159939502787 20.881935910766614 19.311371585215994 9 27.767274789677664 9.417714339729653 21.8990452783817 40.91596559221099 10-11 24.237167974288685 25.683193118442198 28.428254088288117 21.651384818981 12-13 28.42281879194631 27.256120616315343 29.762973816050664 14.558086775687684 14-15 23.70994422913319 26.098638812742227 33.80187163247944 16.389545325645148 16-17 16.593250779846866 27.09896965686738 35.50122884960771 20.80655071367804 18-19 15.53762170337461 21.47603743264959 44.840013233765006 18.146327630210795 20-21 17.881179695623405 20.172038945079876 46.36402306456186 15.582758294734852 22-23 17.08809906418376 20.814349182342376 45.32706304943757 16.7704887040363 24-25 15.690319714339054 21.163912382815916 46.985648420570044 16.160119482274986 26-27 16.377493146800266 20.75219775025995 46.61617355137537 16.25413555156442 28-29 15.791936364345316 20.302061400088384 47.25859546884268 16.64740676672362 30-31 16.439171944418188 21.394271670290195 46.57080064278287 15.595755742508743 32-33 15.3348615181019 21.480527460062387 46.05917383495605 17.125437186879665 34-35 16.136960338785183 21.313737862127475 46.046994155861036 16.502307643226306 36-37 17.013186501559694 21.21845164949428 43.90608753190283 17.862274317043198 38-39 17.450846015691464 21.586633897343795 43.30891388600057 17.653606200964177 40-41 17.41941582380187 21.944654504206447 42.569240949050005 18.066688722941677 42-43 17.139197489288595 22.66966326123073 41.62879566675411 18.56234358272657 44-45 17.980842178404554 23.31104635360905 39.36050785007457 19.34760361791183 46-47 18.627411589811704 24.116488975758248 35.90293845852054 21.353160975909507 48-49 19.38074451516233 25.02759257990031 33.203268095887466 22.388394809049895 50-51 20.069719350073857 25.39698670605613 32.34505169867061 22.18824224519941 52-53 19.537211880008794 26.154075215477 31.3862609924586 22.922451912055607 54-55 19.373059125477983 26.09008021099699 32.050130691459444 22.486729972065586 56-57 20.424112945536013 25.869093590331378 30.73247278691314 22.974320677219467 58-59 19.962188349285125 25.119224861160344 31.60392295876167 23.314663830792863 60-61 20.781470444356742 25.54265498604517 31.221242535170664 22.454632034427426 62-63 21.79805604066576 25.143150178302616 31.34723046056192 21.711563320469708 64-65 21.284434456079158 25.266625547371085 32.16923189628485 21.279708100264912 66-67 21.08853986964557 25.383193825415805 31.845616489505 21.682649815433624 68-69 22.031643251290898 25.55281161749241 31.392515744821637 21.023029386395056 70-71 20.91394838273627 25.729700604745805 31.718787294430147 21.637563718087787 72-73 20.968412793692956 25.126552064771968 31.804861678947695 22.10017346258738 74-75 20.21987059207199 25.90710798330647 31.913611464275753 21.95940996034578 76-77 19.89181371541194 25.75722128088969 33.02068489622104 21.330280107477332 78-79 19.769354381321484 25.10327063049437 32.911664618583984 22.21571036960015 80-81 18.520785429521432 26.59840201154654 32.71126319545701 22.16954936347502 82-83 18.91010492485112 25.830418754135554 31.619009358162398 23.64046696285093 84-85 18.968711598449758 25.359202193023915 31.498723886945832 24.17336232158049 86-87 19.17482553839101 25.7130298540265 31.16748078145567 23.94466382612682 88-89 20.047311380726637 24.208916848239646 32.067048875822984 23.676722895210727 90-91 19.195048634546126 25.204888977114827 31.745266521093875 23.854795867245176 92-93 19.012520146139266 25.413675270230033 31.46247973570156 24.11132484792914 94-95 18.869783863237846 25.49095137041011 31.756932398773035 23.88233236757901 96-97 19.305028429366143 25.532548433903496 30.854251643609654 24.30817149312071 98-99 19.492805168840437 24.931761947862583 30.385134384652286 25.190298498644694 100-101 19.75980546227523 25.241458843880636 30.85828664065583 24.140449053188295 102-103 20.077655310621243 24.867896169697886 30.959796952395358 24.094651567285513 104-105 19.537948179867474 24.590931004168677 31.339742317254153 24.531378498709696 106-107 19.217408154873596 25.41036681334159 30.35414667807296 25.018078353711854 108-109 19.341947924888576 25.26219520840915 29.4126543749616 25.983202491740677 110-111 19.3529557727074 25.186279259373045 29.878413347354986 25.58235162056457 112-113 19.660089196451995 24.496408743804817 29.40764952931973 26.43585253042346 114-115 18.984295965736653 25.038763720939027 29.48453705717176 26.492403256152556 116-117 18.750029545100006 24.563796143537186 29.952586023513174 26.733588287849635 118-119 18.434039053304783 25.408991430205567 29.681598812613146 26.475370703876493 120-121 18.452478015602363 25.367194386741065 28.96244986895621 27.217877728700362 122-123 18.663354871033764 25.156097122897236 28.47064995958727 27.70989804648173 124-125 17.71666383075265 25.270829788440814 28.610118541205832 28.402387839600703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5.0 1 6.5 2 22.5 3 55.5 4 69.5 5 77.5 6 104.0 7 235.5 8 375.0 9 518.5 10 606.0 11 613.0 12 665.5 13 691.0 14 729.5 15 797.5 16 911.0 17 1002.0 18 1090.5 19 1197.5 20 1351.5 21 1516.5 22 1712.0 23 1936.0 24 2098.5 25 2298.5 26 2673.0 27 3066.0 28 3288.0 29 3648.0 30 4090.5 31 4403.0 32 4809.5 33 5407.0 34 6313.5 35 7158.5 36 7539.5 37 8056.0 38 8282.0 39 7858.5 40 7326.0 41 6725.5 42 6314.0 43 6211.0 44 6259.0 45 6303.5 46 6307.5 47 6480.0 48 7041.0 49 6522.0 50 5996.5 51 5533.5 52 4138.5 53 3417.0 54 3025.5 55 2659.0 56 2511.0 57 2431.5 58 2545.5 59 2650.0 60 2982.5 61 3131.5 62 2335.5 63 1280.0 64 677.0 65 546.5 66 425.0 67 340.5 68 351.0 69 313.5 70 264.5 71 263.5 72 200.5 73 220.5 74 208.5 75 134.0 76 104.5 77 73.0 78 37.0 79 10.5 80 4.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.068531997353247 2 4.726344645051517E-4 3 0.0 4 0.0 5 0.07656678324983458 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.3631723225257586E-4 26-27 0.0 28-29 2.3631723225257586E-4 30-31 0.0 32-33 0.0 34-35 2.3631723225257586E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.003072124019283486 44-45 0.010161640986860763 46-47 0.011106909915871065 48-49 0.00921637205785046 50-51 0.008271103128840154 52-53 0.0068531997353246995 54-55 0.006144248038566972 56-57 0.004253710180546365 58-59 0.0023631723225257585 60-61 0.0021268550902731826 62-63 7.089516967577276E-4 64-65 2.3631723225257586E-4 66-67 0.0014179033935154551 68-69 0.0011815861612628792 70-71 0.001654220625768031 72-73 0.003308441251536062 74-75 4.726344645051517E-4 76-77 2.3631723225257586E-4 78-79 0.0 80-81 7.089516967577276E-4 82-83 0.0 84-85 0.0 86-87 2.3631723225257586E-4 88-89 0.0014179033935154551 90-91 9.452689290103034E-4 92-93 4.726344645051517E-4 94-95 4.726344645051517E-4 96-97 0.0014179033935154551 98-99 0.0021268550902731826 100-101 7.089516967577276E-4 102-103 0.001890537858020607 104-105 9.452689290103034E-4 106-107 4.726344645051517E-4 108-109 4.726344645051517E-4 110-111 0.0011815861612628792 112-113 0.011579544380376217 114-115 0.019850647509216373 116-117 0.01843274411570092 118-119 0.010870592683618488 120-121 0.0044900274127989415 122-123 0.006144248038566972 124-125 0.003781075716041214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 211580.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.38198317421306 #Duplication Level Percentage of deduplicated Percentage of total 1 62.53166530262286 22.750259948955478 2 13.49364095768866 9.818508365630022 3 8.020577575119841 8.754135551564419 4 5.067747508996193 7.374988184138386 5 3.293191472777583 5.990641837602798 6 2.10582381750393 4.596842801777106 7 1.312080231757538 3.341525664051423 8 0.8820816607558102 2.5673504111919843 9 0.5274302713797627 1.7270063333018244 >10 2.4773633682788363 16.023253615653655 >50 0.17018070332696777 4.303809433783911 >100 0.1052262364082778 7.176009074581718 >500 0.0077945360302428 1.6003402968144436 >1k 0.0051963573534952 3.975328480952831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3476 1.6428773986199074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.9306172606106438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.9074581718498913 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1046 0.49437564987238863 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 584 0.2760185272710086 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 581 0.2746006238774931 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 579 0.2736553549484828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 567 0.26798374137442105 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 539 0.25474997636827673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 536 0.25333207297476135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 451 0.21315814349182344 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 404 0.1909443236600813 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 397 0.18763588240854523 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 393 0.18574534455052463 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 382 0.18054636544096797 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 362 0.17109367615086493 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 327 0.15455146989318463 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 324 0.15313356649966917 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.15218829757065885 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 313 0.14793458739011248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 302 0.14273560828055581 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 280 0.13233765006144246 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 272 0.12855657434540124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 259 0.12241232630683428 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 256 0.12099442291331884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 254 0.12004915398430853 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.11485017487475188 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 240 0.11343227148123641 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 234 0.11059646469420549 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 233 0.11012383022970035 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 231 0.10917856130069005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 215 0.10161640986860762 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.726344645051517E-4 0.0 18-19 0.0 0.0 0.0 7.089516967577276E-4 0.0 20-21 0.0 0.0 0.0 9.452689290103034E-4 0.0 22-23 0.0 0.0 0.0 0.001654220625768031 0.0 24-25 0.0 0.0 0.0 0.001890537858020607 0.0 26-27 0.0 0.0 0.0 0.0028358067870309102 0.0 28-29 0.0 0.0 0.0 0.00401739294829379 0.0 30-31 0.0 0.0 0.0 0.010634275451365914 0.0 32-33 0.0 0.0 0.0 0.034974950373381226 0.0 34-35 0.0 0.0 0.0 0.068531997353247 0.0 36-37 0.0 0.0 0.0 0.11886756782304565 0.0 38-39 0.0 0.0 0.0 0.1810189999054731 0.0 40-41 0.0 0.0 0.0 0.26183949333585405 0.0 42-43 0.0 0.0 0.0 0.34195103506947727 0.0 44-45 0.0 0.0 0.0 0.4456943000283581 0.0 46-47 0.0 0.0 0.0 0.5591265715095945 0.0 48-49 0.0 0.0 0.0 0.6654693260232536 0.0 50-51 0.0 0.0 0.0 0.7848095283108044 0.0 52-53 0.0 0.0 0.0 0.9072218546176387 0.0 54-55 0.0 0.0 0.0 1.0598827866528027 0.0 56-57 0.0 0.0 0.0 1.2432649588808016 0.0 58-59 0.0 0.0 0.0 1.4779279705076096 0.0 60-61 0.0 0.0 0.0 1.7057377823990925 0.0 62-63 0.0 0.0 0.0 1.9110974572265809 0.0 64-65 0.0 0.0 0.0 2.155685792607997 0.0 66-67 0.0 0.0 0.0 2.445410719349655 0.0 68-69 0.0 0.0 0.0 2.7873617544191323 0.0 70-71 0.0 0.0 0.0 3.148690802533321 0.0 72-73 0.0 0.0 0.0 3.473626996880612 0.0 74-75 0.0 0.0 0.0 3.8413366102656203 0.0 76-77 0.0 0.0 0.0 4.2693071178750355 0.0 78-79 0.0 0.0 0.0 4.787550808204934 0.0 80-81 0.0 0.0 0.0 5.294451271386709 0.0 82-83 0.0 0.0 0.0 5.785991114472067 0.0 84-85 0.0 0.0 0.0 6.295963701673126 0.0 86-87 0.0 0.0 0.0 6.93851025616788 0.0 88-89 0.0 0.0 0.0 7.629265526042159 0.0 90-91 0.0 0.0 0.0 8.289535872955856 0.0 92-93 0.0 0.0 0.0 8.959258909159654 0.0 94-95 0.0 0.0 0.0 9.64127044144059 0.0 96-97 0.0 0.0 0.0 10.407883542867946 0.0 98-99 0.0 0.0 0.0 11.240192834861517 0.0 100-101 0.0 0.0 0.0 12.153795254749976 0.0 102-103 0.0 0.0 0.0 13.013044711220342 0.0 104-105 0.0 0.0 0.0 13.839209755175347 0.0 106-107 0.0 0.0 0.0 14.719727762548445 0.0 108-109 0.0 0.0 0.0 15.64869080253332 0.0 110-111 0.0 0.0 0.0 16.6927403346252 0.0 112-113 0.0 0.0 0.0 17.693071178750355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 25 1.2011515E-7 119.0402 3 TATAACG 25 1.7195605E-5 95.232155 2 TCACGCA 30 4.2490356E-5 79.36013 4 TAACGCA 40 1.972232E-6 74.40013 4 CACGCAG 70 1.72804E-10 68.02297 5 ATCACGC 35 9.11993E-5 68.02297 3 GAAACTA 40 1.7698434E-4 59.491966 9 GAGCCGA 15 0.00423697 59.491962 22-23 GGTATCA 3345 0.0 53.075584 1 TATCACG 50 5.31382E-4 47.616077 2 CCACGCA 50 5.31382E-4 47.616077 4 GTCGGGA 335 0.0 44.417984 2 GTTATCA 135 2.7284841E-11 44.1307 1 CGGGAGT 355 0.0 43.592186 4 CAGGACT 55 8.498455E-4 43.287346 4 GTAGGAC 85 3.4593395E-6 42.014187 3 GATCAGT 85 3.4706118E-6 41.99433 6 GACATAT 100 2.1918277E-7 41.703514 1 ATGGGTA 130 9.058567E-10 41.206223 5 CATGGGG 805 0.0 40.66591 4 >>END_MODULE