FastQCFastQC Report
Thu 26 May 2016
SRR936223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17457
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.6530331672108609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1030.5900211949361287No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT910.5212808615455119No Hit
GTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAGCA570.32651658360543045No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT520.29787477802600676No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG510.292146416910122No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.28068969467835253No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.2635046113306983No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.23486280575127455No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.22340608351950506No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.22340608351950506No Hit
GAGTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAG300.17185083347654237No Hit
GCTTTAGGGGTGCAAATGGTTTTTGGTTACATGGATGCATTGTATAGTAG290.1661224723606576No Hit
GTGATATCCCCTTTATCATTTTTCATTGCGTCTATTTGATTCTTCTCTCT290.1661224723606576No Hit
GTGTTTATAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATCGGTGG270.1546657501288881No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.14320902789711865No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC240.13748066678123388No Hit
GTATAGTAGATGCATCTGGGCTTTCATTGTACCCCTCACCTGAATAGTGT240.13748066678123388No Hit
GTATGGGAGAAGGGAGAGCCGGACCCTGGATCCAGAAGAAATGTGACTGT240.13748066678123388No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13175230566534915No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG230.13175230566534915No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT230.13175230566534915No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13175230566534915No Hit
GTACATGGGACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGT220.1260239445494644No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12029558343357966No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG210.12029558343357966No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11456722231769491No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.11456722231769491No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG190.10883886120181016No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC190.10883886120181016No Hit
AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10311050008592541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA205.542215E-6118.979952
TACAAGA152.6585857E-4118.9799352
ACATGAA303.4351433E-799.149953
CATGAAA251.679723E-595.183954
CAAGAGA208.346828E-489.234964
GTACAAG208.346828E-489.234961
GAGAACT250.002024315871.387969
CAACTAA250.002024315871.387966
CGCAGAA508.4351996E-871.387962
ACAAGAG358.9102105E-567.988543
GCAGAAA701.24364306E-859.489973
TCATCTC150.004205068659.48996784-85
AGAGAAC655.1798634E-754.913825
AACAAGA551.2590297E-554.0817872
ATGGGAG453.0876088E-452.8799745
AAGAGAA453.0876088E-452.8799745
ATGAAAA350.007674076650.99145
GCAGAGA855.750917E-848.9917453
CAGAGAA751.3902772E-647.5919764
AGAACAT653.3822027E-545.7615177
Produced by FastQC (version 0.11.5)