##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17457 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.487884516239905 26.0 12.0 31.0 2.0 34.0 2 21.62679727330011 26.0 10.0 31.0 2.0 34.0 3 21.951595348570773 26.0 10.0 31.0 2.0 34.0 4 24.08237383284642 30.0 10.0 35.0 2.0 37.0 5 25.693704531133644 31.0 16.0 35.0 2.0 37.0 6 24.16497680013748 31.0 8.0 37.0 2.0 37.0 7 24.803345362891676 31.0 8.0 36.0 2.0 37.0 8 23.888010540184453 31.0 8.0 37.0 2.0 37.0 9 23.85484332932348 30.0 8.0 39.0 2.0 39.0 10-11 24.581342727845563 31.0 8.0 38.0 2.0 39.0 12-13 24.648020851234463 31.0 7.5 38.0 2.0 39.0 14-15 25.278913902732427 31.0 8.0 39.0 2.0 41.0 16-17 25.481010482900842 31.0 8.0 39.0 2.0 41.0 18-19 25.58856046285158 31.0 8.0 39.0 2.0 41.0 20-21 25.92263848312998 31.0 9.0 39.0 2.0 41.0 22-23 26.018731740848942 31.0 10.5 39.0 2.0 41.0 24-25 26.100360886750302 30.5 11.5 39.0 2.0 41.0 26-27 25.80105401844532 30.0 4.5 39.0 2.0 41.0 28-29 25.26731397147276 30.0 2.0 39.0 2.0 41.0 30-31 24.794523686773214 30.0 2.0 39.0 2.0 41.0 32-33 24.215500945179585 30.0 2.0 38.0 2.0 41.0 34-35 23.709600733230225 30.0 2.0 38.0 2.0 41.0 36-37 23.197743025720342 30.0 2.0 38.0 2.0 41.0 38-39 22.591682419659737 29.5 2.0 38.0 2.0 41.0 40-41 22.30308758664146 29.0 2.0 38.0 2.0 40.5 42-43 21.883599702125224 28.0 2.0 38.0 2.0 41.0 44-45 21.648335911095835 28.5 2.0 38.0 2.0 40.5 46-47 21.42289625938019 28.5 2.0 38.0 2.0 40.0 48-49 21.124133585381223 28.0 2.0 38.0 2.0 40.0 50-51 20.810133470814 28.0 2.0 38.0 2.0 40.0 52-53 20.554247579767427 27.0 2.0 38.0 2.0 40.0 54-55 20.304118691642323 27.0 2.0 38.0 2.0 40.0 56-57 20.0467720685112 26.0 2.0 37.0 2.0 40.0 58-59 19.832817780832904 26.0 2.0 37.0 2.0 40.0 60-61 19.567766512000915 25.5 2.0 37.0 2.0 40.0 62-63 19.266626568138854 23.5 2.0 36.0 2.0 40.0 64-65 18.98307269290256 22.5 2.0 36.0 2.0 40.0 66-67 18.736495388669304 22.0 2.0 35.0 2.0 39.5 68-69 18.518817666265683 20.5 2.0 35.0 2.0 39.0 70-71 18.17944091195509 17.5 2.0 35.0 2.0 39.0 72-73 17.812768516927306 12.5 2.0 35.0 2.0 38.5 74-75 17.571203528670445 7.5 2.0 34.0 2.0 37.5 76-77 17.25210517271009 2.0 2.0 34.0 2.0 37.0 78-79 16.963052070802544 2.0 2.0 34.0 2.0 37.0 80-81 16.707538523228507 2.0 2.0 34.0 2.0 36.0 82-83 16.505699719310304 2.0 2.0 34.0 2.0 36.0 84-85 16.45423039468408 2.0 2.0 34.0 2.0 35.0 86-87 16.321475625823453 2.0 2.0 34.0 2.0 35.0 88-89 16.149281090679956 2.0 2.0 34.0 2.0 35.0 90-91 16.005842928338204 2.0 2.0 34.0 2.0 35.0 92-93 15.884458956292605 2.0 2.0 34.0 2.0 35.0 94-95 15.752878501460732 2.0 2.0 34.0 2.0 35.0 96-97 15.68390903362548 2.0 2.0 34.0 2.0 35.0 98-99 15.597639915220256 2.0 2.0 34.0 2.0 35.0 100-101 15.455605201351894 2.0 2.0 34.0 2.0 35.0 102-103 14.888869794351837 2.0 2.0 32.0 2.0 34.5 104-105 15.219854499627656 2.0 2.0 33.0 2.0 35.0 106-107 15.225411009910065 2.0 2.0 34.0 2.0 35.0 108-109 15.138454488170934 2.0 2.0 34.0 2.0 35.0 110-111 15.0775333677035 2.0 2.0 33.5 2.0 35.0 112-113 14.981497393595692 2.0 2.0 34.0 2.0 35.0 114-115 14.838001947642779 2.0 2.0 33.0 2.0 35.0 116-117 14.678982643065819 2.0 2.0 33.0 2.0 35.0 118-119 14.504410838059231 2.0 2.0 33.0 2.0 35.0 120-121 14.287907429684367 2.0 2.0 33.0 2.0 35.0 122-123 14.060061866300051 2.0 2.0 33.0 2.0 35.0 124-125 12.917110614653147 2.0 2.0 30.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.2190722464843375 1101 2 2.7448000386580738 1101 3 2.392615297891531 1101 4 3.979579843670514 1101 5 0.04185556708755911 1101 6 2.158259366941923 1101 7 3.126912216316903 1101 8 2.939428283239156 1101 9 4.151693453644196 1101 10-11 2.758262903235753 1101 12-13 3.7646453632624066 1101 14-15 2.1080183565008994 1101 16-17 4.4235750288508555 1101 18-19 2.993498393445112 1101 20-21 1.0710886591591624 1101 22-23 1.8050887721307056 1101 24-25 4.269031943968617 1101 26-27 4.614954530363793 1101 28-29 4.456465206083241 1101 30-31 3.666574193818189 1101 32-33 4.137919329468179 1101 34-35 5.967157839831074 1101 36-37 6.207586915887145 1101 38-39 7.55371910851493 1101 40-41 7.263737899781461 1101 42-43 6.018957270492102 1101 44-45 5.561423891415117 1101 46-47 5.426380949839341 1101 48-49 5.66445665075225 1101 50-51 5.095428201645554 1101 52-53 3.8107481211049254 1101 54-55 3.9900392453572273 1101 56-57 3.334402062069781 1101 58-59 4.205860614636396 1101 60-61 4.359692772101752 1101 62-63 5.511642955218608 1101 64-65 5.0928360727286375 1101 66-67 5.252108300548411 1101 68-69 5.1904528163787305 1101 70-71 5.875618986624215 1101 72-73 6.103962126587355 1101 74-75 4.748808466010779 1101 76-77 3.929692207259478 1101 78-79 3.2881215562195365 1101 80-81 2.9869919357636334 1101 82-83 2.560947823824481 1101 84-85 2.5816771777898353 1101 86-87 2.7823623690231365 1101 88-89 2.2338641386167133 1101 90-91 2.593763895790161 1101 92-93 3.042210182031983 1101 94-95 2.2648698608219533 1101 96-97 3.2087683745453788 1101 98-99 2.974227940671625 1101 100-101 2.844009276590347 1101 102-103 2.5596674807338644 1101 104-105 2.99551167352198 1101 106-107 3.3300587318097605 1101 108-109 3.4898716334493756 1101 110-111 2.9910344535334925 1101 112-113 2.226137283082444 1101 114-115 1.9239593979175869 1101 116-117 2.136614936268698 1101 118-119 2.3617118515237934 1101 120-121 2.0160944321291208 1101 122-123 2.397699637416867 1101 124-125 2.3041932277775405 1102 1 2.4796542570663505 1102 2 1.8783979222559566 1102 3 0.9481708534470883 1102 4 2.37375973785041 1102 5 -1.309996284764292 1102 6 1.7323334410159958 1102 7 1.141462480867169 1102 8 2.9870473308582035 1102 9 3.2667728187235596 1102 10-11 2.6550883000611485 1102 12-13 2.8235077971248366 1102 14-15 1.7151612136437535 1102 16-17 2.606114711390539 1102 18-19 3.3486571236038394 1102 20-21 1.1974114104819158 1102 22-23 1.2085279255698573 1102 24-25 0.9211483460850189 1102 26-27 0.27368468909395105 1102 28-29 1.6356980103160446 1102 30-31 1.495939273183268 1102 32-33 0.3522050437538944 1102 34-35 0.3732424959157292 1102 36-37 1.9403911486913792 1102 38-39 2.315623870419696 1102 40-41 3.369558005601565 1102 42-43 3.42768742922226 1102 44-45 3.1434344734256996 1102 46-47 4.124793648252041 1102 48-49 3.72199633329193 1102 50-51 3.6311424873598384 1102 52-53 3.1202719306287356 1102 54-55 2.9569704622884494 1102 56-57 2.634005236672955 1102 58-59 3.50678654056232 1102 60-61 2.9536081160170973 1102 62-63 4.3244736430492985 1102 64-65 4.074978929871495 1102 66-67 4.662161210601322 1102 68-69 3.306193557119471 1102 70-71 3.226809462814689 1102 72-73 4.088750486375716 1102 74-75 3.830819048021361 1102 76-77 4.137364164931437 1102 78-79 4.423703566801549 1102 80-81 4.268737967509665 1102 82-83 4.440577453454111 1102 84-85 3.80125389736655 1102 86-87 3.283023744684513 1102 88-89 3.6492080539606278 1102 90-91 3.696277123303389 1102 92-93 4.027659917481721 1102 94-95 3.8362984322505227 1102 96-97 3.4779482687252745 1102 98-99 3.9550480464917293 1102 100-101 4.086072768653841 1102 102-103 3.2554346765010607 1102 104-105 2.949876752887059 1102 106-107 3.451090477841504 1102 108-109 3.462093855671597 1102 110-111 3.3600820725811094 1102 112-113 3.5164811984263586 1102 114-115 3.912716011674201 1102 116-117 3.842964142617907 1102 118-119 3.529039893851831 1102 120-121 3.8970468130815004 1102 122-123 3.135794875512108 1102 124-125 2.5647752383595517 1103 1 1.8946788876082223 1103 2 2.209678710433298 1103 3 0.9136880948263979 1103 4 2.637306535879965 1103 5 -0.2927549054539469 1103 6 2.749574820326341 1103 7 2.223974796138105 1103 8 3.376209892434556 1103 9 3.6842605034526237 1103 10-11 2.1969602212434154 1103 12-13 1.792103856238139 1103 14-15 0.3666390461560738 1103 16-17 0.8795137261688666 1103 18-19 0.5758738723723127 1103 20-21 0.8667463858513749 1103 22-23 1.5496609304959712 1103 24-25 2.0800153411589086 1103 26-27 0.6283644920496165 1103 28-29 0.04394924184314064 1103 30-31 -1.369824273614764 1103 32-33 1.2930917432612823 1103 34-35 1.3030454515807541 1103 36-37 0.6398985378539415 1103 38-39 1.6973972694344717 1103 40-41 1.8473905179168355 1103 42-43 0.6530568873503384 1103 44-45 1.2025477739183117 1103 46-47 2.4819365053948985 1103 48-49 2.1203953480702573 1103 50-51 2.5437040637145216 1103 52-53 3.063621684323312 1103 54-55 2.305492629776129 1103 56-57 1.8957047440621189 1103 58-59 1.2697175750450818 1103 60-61 1.0065637810417272 1103 62-63 1.2536608351675227 1103 64-65 1.6962843485907086 1103 66-67 1.8715208165126498 1103 68-69 1.569124591602229 1103 70-71 1.6227454234058243 1103 72-73 3.0056224075579827 1103 74-75 2.889932348513973 1103 76-77 2.7962311600053305 1103 78-79 2.446486818033076 1103 80-81 2.168984272928384 1103 82-83 2.345749867247214 1103 84-85 2.264308084558671 1103 86-87 2.766398128920965 1103 88-89 3.087631699280827 1103 90-91 2.722754955815706 1103 92-93 3.3072165677280267 1103 94-95 3.2821112401322985 1103 96-97 2.797529549513449 1103 98-99 1.7820184898414837 1103 100-101 1.8804077440232998 1103 102-103 2.3170110311808614 1103 104-105 2.6142856198821303 1103 106-107 2.1844648620779594 1103 108-109 2.160369717740565 1103 110-111 2.979540200659928 1103 112-113 3.4936979471948355 1103 114-115 2.5919032037924286 1103 116-117 3.4661168519775085 1103 118-119 3.3744832436055283 1103 120-121 4.044830064313027 1103 122-123 4.219538717876638 1103 124-125 3.7384205585565944 1104 1 -0.1512981238860327 1104 2 0.5596148534728869 1104 3 0.8740967793730157 1104 4 2.0536539177445903 1104 5 -0.32851480328281113 1104 6 0.38048158916414465 1104 7 0.5343196237243113 1104 8 1.1986875424984156 1104 9 3.003545305496047 1104 10-11 2.369374014346864 1104 12-13 1.3387194373364757 1104 14-15 0.5154257639083042 1104 16-17 1.0717231769990079 1104 18-19 0.6786835786302952 1104 20-21 1.8118293998999029 1104 22-23 1.4717554387973735 1104 24-25 2.8431060180426933 1104 26-27 1.4668063822156405 1104 28-29 1.530539280157317 1104 30-31 0.8887964160404103 1104 32-33 1.9527341442829957 1104 34-35 2.2078985805718183 1104 36-37 1.5964758047760341 1104 38-39 2.9055709603667843 1104 40-41 2.634108270151831 1104 42-43 2.8708091223439496 1104 44-45 1.3021646321558578 1104 46-47 2.093047616506013 1104 48-49 2.4051973914929903 1104 50-51 2.280613386830737 1104 52-53 3.051488861845666 1104 54-55 3.360409615727601 1104 56-57 3.0936613213290407 1104 58-59 0.5762309850067631 1104 60-61 0.4152483276573058 1104 62-63 1.344976288551944 1104 64-65 0.7595027393953053 1104 66-67 1.3817379301780406 1104 68-69 2.0608231867491007 1104 70-71 2.283026394031623 1104 72-73 0.9187769414021716 1104 74-75 2.3599195771218895 1104 76-77 2.33709961466689 1104 78-79 3.232566000663983 1104 80-81 2.6536586024303013 1104 82-83 3.542429305305962 1104 84-85 2.7298253259379806 1104 86-87 3.4305105171712853 1104 88-89 2.7338641386167133 1104 90-91 3.0196898217160886 1104 92-93 3.523691663513464 1104 94-95 2.6722772682293616 1104 96-97 2.3569165226935276 1104 98-99 2.418672385116068 1104 100-101 2.1588240914051635 1104 102-103 1.874482295548681 1104 104-105 2.939956117966423 1104 106-107 1.8485772503282796 1104 108-109 2.65653830011604 1104 110-111 3.009552972052008 1104 112-113 3.355766912712074 1104 114-115 1.9609964349546214 1104 116-117 1.2292075288612931 1104 118-119 2.1394896293015684 1104 120-121 2.201279617314304 1104 122-123 2.6939959337131647 1104 124-125 1.896785820370134 1105 1 -0.3692468418347481 1105 2 0.02400231786035434 1105 3 -0.6287522234759884 1105 4 -3.2910754269847544 1105 5 -0.6561501309181388 1105 6 0.5400257487083024 1105 7 -1.388757299352612 1105 8 -2.3426229988121285 1105 9 -1.2030074010566594 1105 10-11 -1.8998567548839063 1105 12-13 -3.048744950127908 1105 14-15 -3.507366258883714 1105 16-17 -3.599929244653417 1105 18-19 -3.369749469802752 1105 20-21 -2.988028149957646 1105 22-23 -1.0332303161883836 1105 24-25 -1.27940110446443 1105 26-27 1.0786297440390022 1105 28-29 1.3980606476786832 1105 30-31 2.3887964160404103 1105 32-33 2.907150098698949 1105 34-35 4.145220517893751 1105 36-37 3.2837977420979705 1105 38-39 3.771667826463652 1105 40-41 1.7074700935136526 1105 42-43 2.751863253398085 1105 44-45 2.126950956942185 1105 46-47 2.5203980438564386 1105 48-49 -0.6090476227520227 1105 50-51 -1.212264106046753 1105 52-53 -0.7341236737668702 1105 54-55 -1.37819437287639 1105 56-57 -0.5651705875028696 1105 58-59 -0.5797519209761397 1105 60-61 -0.14529298288400483 1105 62-63 -0.190636247060592 1105 64-65 -0.022548542655975723 1105 66-67 0.4258974743375852 1105 68-69 0.23888586481177754 1105 70-71 0.9781830891883132 1105 72-73 -0.610425337800109 1105 74-75 -2.9164336992313835 1105 76-77 -3.12087759331032 1105 78-79 -3.2617359936380126 1105 80-81 -2.0279653291936306 1105 82-83 -2.5017302388535825 1105 84-85 -2.4047900586774027 1105 86-87 -1.506811420150651 1105 88-89 -1.2326600779075036 1105 90-91 -2.8050965030702386 1105 92-93 -3.519043379221575 1105 94-95 -3.3120532161011234 1105 96-97 -2.273425357648353 1105 98-99 -2.447424480980798 1105 100-101 -2.551289868708798 1105 102-103 -1.747312576246193 1105 104-105 -2.5130353350250285 1105 106-107 -3.607975456224427 1105 108-109 -4.153290759713018 1105 110-111 -3.521786059287022 1105 112-113 -3.9519253949802327 1105 114-115 -3.794701570743383 1105 116-117 -3.277914978261217 1105 118-119 -3.2223337325217933 1105 120-121 -2.836469670434985 1105 122-123 -3.07024908053185 1105 124-125 -1.687972014387702 1106 1 -1.3307853033732115 1106 2 -1.9375361436781091 1106 3 0.17894008421631824 1106 4 -2.8295369654462945 1106 5 -2.309996284764292 1106 6 -4.152281943599387 1106 7 -4.042603453198765 1106 8 -2.650315306504435 1106 9 -4.703007401056659 1106 10-11 -3.0344721394992895 1106 12-13 -4.471821873204835 1106 14-15 -2.7189047204221772 1106 16-17 -3.6576215523457236 1106 18-19 -3.773595623648905 1106 20-21 -1.9687973807268762 1106 22-23 -2.2447687777268435 1106 24-25 -5.74093956600289 1106 26-27 -1.6713702559609978 1106 28-29 -0.21732396770593354 1106 30-31 0.3118733391173336 1106 32-33 -3.1890037474548976 1106 34-35 -1.470164097490862 1106 36-37 -0.9469714886712595 1106 38-39 0.3678216726174952 1106 40-41 -0.38868375264019406 1106 42-43 -1.3635213619865318 1106 44-45 -0.1422798122885851 1106 46-47 0.40501342847182187 1106 48-49 0.6986446849402839 1106 50-51 -0.3276487214313697 1106 52-53 -0.6379698276130235 1106 54-55 -0.4743482190302366 1106 56-57 -0.18055520288748284 1106 58-59 0.0356326944084735 1106 60-61 -0.2799083674993881 1106 62-63 -0.690636247060592 1106 64-65 -0.5802408503482859 1106 66-67 -1.112564064123955 1106 68-69 -0.7418833659574489 1106 70-71 -1.0410476800424533 1106 72-73 -0.3219637993385689 1106 74-75 -0.4356644684621571 1106 76-77 0.2637377913050649 1106 78-79 0.10364862174660416 1106 80-81 -0.3356576368859372 1106 82-83 0.11365437653103427 1106 84-85 -0.9047900586774027 1106 86-87 -0.7375806509198828 1106 88-89 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3.426431723012307 2216 4 5.190529924185814 2216 5 4.494351541322665 2216 6 4.884507354059473 2216 7 2.6212761454634403 2216 8 3.0988485730942266 2216 9 4.895654806301199 2216 10-11 4.151982709999036 2216 12-13 2.4562717722801146 2216 14-15 3.021061834761767 2216 16-17 2.9694687486576186 2216 18-19 4.024899359628684 2216 20-21 5.564647435326638 2216 22-23 5.536167677122652 2216 24-25 4.723943377140923 2216 26-27 4.320268540025626 2216 28-29 5.518461985471323 2216 30-31 4.62792685082302 2216 32-33 3.4640062859899174 2216 34-35 1.7288325580275306 2216 36-37 0.4652358691882732 2216 38-39 0.19140026793522225 2216 40-41 0.5310486888313797 2216 42-43 0.8547060627626308 2216 44-45 0.7925027964070672 2216 46-47 -0.27893305982249217 2216 48-49 -0.4200844120496825 2216 50-51 0.42151515816729557 2216 52-53 0.15132783124984783 2216 54-55 1.9119394063878268 2216 56-57 1.1363344292195414 2216 58-59 0.5448300188231912 2216 60-61 0.09801805390529594 2216 62-63 -2.2725760463916984 2216 64-65 -2.6028161011844055 2216 66-67 -1.6359754353614129 2216 68-69 -2.264458616793572 2216 70-71 -1.5301781148250626 2216 72-73 -1.1263116254255259 2216 74-75 -0.7375039333450957 2216 76-77 -0.25549297792570513 2216 78-79 0.04093959164627137 2216 80-81 -0.14585830578225867 2216 82-83 -0.5702921117632798 2216 84-85 0.7080861955031992 2216 86-87 0.45305480058513226 2216 88-89 0.580885072594171 2216 90-91 0.7467429618127035 2216 92-93 0.7434984267984923 2216 94-95 1.2648698608219533 2216 96-97 0.6371097594085029 2216 98-99 1.437190903634587 2216 100-101 0.4043957500202975 2216 102-103 1.4324533100414314 2216 104-105 1.1742556348746351 2216 106-107 0.8059041424377824 2216 108-109 0.603398203497683 2216 110-111 1.4113243086059537 2216 112-113 1.7035929996685972 2216 114-115 2.3482750178853795 2216 116-117 2.392653583611695 2216 118-119 2.3947231236655 2216 120-121 2.0305871857523083 2216 122-123 2.7382793475617966 2216 124-125 1.6004895240738364 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2915.0 3 278.0 4 293.0 5 486.0 6 1107.0 7 925.0 8 512.0 9 428.0 10 388.0 11 300.0 12 256.0 13 243.0 14 228.0 15 202.0 16 167.0 17 132.0 18 112.0 19 112.0 20 81.0 21 106.0 22 103.0 23 141.0 24 198.0 25 252.0 26 246.0 27 224.0 28 247.0 29 286.0 30 323.0 31 367.0 32 425.0 33 560.0 34 678.0 35 961.0 36 1292.0 37 1419.0 38 459.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.24757976742853 20.66792690611216 9.016440396402588 16.068052930056712 2 18.015695709457525 32.91516297187375 29.048519218651542 20.020622100017185 3 19.172824654866243 43.884974508793036 21.177751045425904 15.764449790914819 4 10.505814286532624 28.739187718393765 24.906914131866873 35.84808386320674 5 7.859311450993871 58.045483187260125 23.245689408260297 10.849515953485708 6 23.703958297531077 42.25239159076588 14.882282179068568 19.161367932634473 7 22.48954574096351 48.519218651543795 14.670332817780832 14.320902789711864 8 22.254682935212237 48.41610815145787 13.776708483702812 15.552500429627084 9 23.990376353325313 30.079624219510798 11.462450592885375 34.46754883427851 10-11 18.23910179297703 44.142750759007846 17.75791945924271 19.86022798877241 12-13 21.00017185083348 44.44921807870768 17.800882167611846 16.749727902846995 14-15 22.601248782723264 43.83341925875007 16.42893968035745 17.136392278169215 16-17 20.0893624334078 42.31826774359856 19.662599530274388 17.92977029271925 18-19 21.063183823108208 38.872658532393885 22.509595004869105 17.554562639628802 20-21 23.749785186458155 37.02239789196311 22.131523171220714 17.096293750358022 22-23 22.46015365210412 37.309368191721134 21.155830753353975 19.074647402820776 24-25 20.352867044738502 38.08214469840179 20.381508850317925 21.18347940654179 26-27 20.99157930915965 37.14555765595463 21.48994672624162 20.372916308644097 28-29 21.08896144812969 38.89843615741536 21.9482156155124 18.064386778942545 30-31 20.430199919802945 41.295755284413126 21.31523171220714 16.95881308357679 32-33 18.72028412671135 40.983559603597406 22.045597754482444 18.250558515208798 34-35 17.78369708426419 42.567451452139544 21.31523171220714 18.333619751389126 36-37 18.53411239044509 42.30394684080884 20.470298447614137 18.691642321131923 38-39 18.59139600160394 42.42997078535831 20.518989517099158 18.459643695938592 40-41 18.25342269576674 43.9164804949304 20.7108896144813 17.119207194821563 42-43 17.969868820530447 44.88170934295698 20.40155811422352 16.746863722289053 44-45 17.921177751045427 44.389070286990886 20.22111473907315 17.468637222890532 46-47 16.835653319585266 44.79578392621871 20.264077447442286 18.104485306753737 48-49 17.30824311164576 44.096923870080765 19.536575585724925 19.058257432548547 50-51 17.875350862118346 43.36082946668958 20.433064100360887 18.330755570831187 52-53 17.225181875465427 43.20902789711864 20.146646044566648 19.419144182849287 54-55 17.173626625422468 43.20616371656069 18.87208569628229 20.748123961734546 56-57 16.81560405567967 41.03511485364037 19.805808558171506 22.34347253250845 58-59 17.216589333791603 39.28796471329552 20.215386377957266 23.280059574955605 60-61 16.66953084722461 38.54900612934639 20.567680586584178 24.21378243684482 62-63 17.01896087529358 36.4065990720055 20.567680586584178 26.006759466116748 64-65 16.735407000057283 34.97164461247637 20.914246434095205 27.37870195337114 66-67 17.60038952855588 33.32760497221745 22.011227587787133 27.060777911439537 68-69 18.708827404479578 33.201581027667984 22.17735006014779 25.912241507704646 70-71 18.943690210230855 33.29609898608008 22.165893337916025 25.59431746577304 72-73 18.766111015638426 33.029730194191444 23.133986366500544 25.07017242366959 74-75 19.066849974222375 31.041988886979432 24.694964770579137 25.196196368219052 76-77 19.739932405338834 29.618491149682075 26.05258635504382 24.58899008993527 78-79 20.5218536976571 30.096809302858453 25.284985965515265 24.09635103396918 80-81 19.596723377441712 29.54975081629146 26.98917339749098 23.86435240877585 82-83 20.696568711691583 29.552614996849403 25.995302743884974 23.75551354757404 84-85 21.083233087013806 30.002291344446352 25.539898035172136 23.374577533367706 86-87 21.446984017872488 30.010883886120183 25.07590078478547 23.46623131122186 88-89 21.060319642550265 30.128315288995815 25.708884688090738 23.102480380363176 90-91 21.109010712035285 31.454430887323138 25.007160451394856 22.42939794924672 92-93 20.23257146130492 32.61155983273186 23.526379102938648 23.629489603024574 94-95 18.989517099157933 33.86320673655267 23.440453686200378 23.706822478089016 96-97 19.49361287735579 35.67050466861431 23.211319241564986 21.624563212464913 98-99 18.207595806839663 38.162341754024176 21.7992782264994 21.830784212636765 100-101 17.302514750529873 40.078478547287624 21.074640545339978 21.544366156842525 102-103 16.053732027267 44.41484791201237 19.96620266941628 19.565217391304348 104-105 15.652746749155067 46.63745202497566 18.88067823795612 18.82912298791316 106-107 15.045540470871282 47.181646330984705 18.96373947413645 18.80907372400756 108-109 14.764850776192931 48.8371426934754 17.9870539038781 18.41095262645357 110-111 15.05126883198717 48.56790972102881 18.147448015122876 18.233373431861143 112-113 14.839319470699433 48.28722002635046 18.522655668213325 18.35080483473678 114-115 15.598327318554162 48.066678123388904 18.3994959042218 17.93549865383514 116-117 15.88760955490634 47.2217448587959 19.003838001947642 17.886807584350116 118-119 16.277138110786503 46.51715644154207 18.791888640659906 18.413816807011514 120-121 16.168299249584695 45.08220198201295 19.45924271066048 19.290256057741882 122-123 16.918714555765597 43.45534742510168 19.424872543965172 20.201065475167553 124-125 17.047602680873002 44.205762731282576 18.728876668385176 20.017757919459243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12.0 1 18.5 2 27.0 3 29.5 4 34.5 5 43.0 6 44.5 7 38.5 8 52.0 9 55.0 10 49.0 11 55.0 12 53.5 13 51.5 14 48.5 15 44.0 16 42.5 17 46.0 18 47.5 19 54.0 20 53.0 21 64.5 22 93.5 23 124.0 24 164.5 25 212.0 26 245.5 27 290.5 28 338.5 29 387.0 30 420.0 31 439.0 32 513.0 33 556.0 34 559.0 35 593.5 36 638.5 37 602.0 38 528.0 39 539.0 40 539.0 41 470.0 42 433.0 43 446.5 44 439.0 45 425.5 46 433.0 47 385.5 48 379.0 49 460.5 50 624.5 51 650.0 52 472.5 53 337.0 54 268.5 55 262.0 56 242.5 57 242.0 58 238.0 59 217.0 60 183.0 61 150.5 62 134.0 63 98.0 64 84.0 65 85.0 66 77.0 67 56.5 68 49.5 69 50.0 70 50.5 71 49.5 72 40.0 73 33.5 74 35.5 75 30.5 76 17.5 77 12.0 78 11.5 79 10.5 80 7.5 81 6.0 82 5.5 83 4.0 84 4.0 85 2.5 86 0.5 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.08592541673827118 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 17457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.37944664031622 #Duplication Level Percentage of deduplicated Percentage of total 1 84.22551729915389 57.592942659105226 2 6.911284242271927 9.45179584120983 3 3.7781687191086535 7.750472589792061 4 2.3372706710228703 6.392851005327375 5 0.9550138225684845 3.2651658360543045 6 0.48588422551729915 1.9934696683278914 7 0.33509256932227527 1.6039411124477285 8 0.17592359889419454 0.9623646674686372 9 0.12565971349585323 0.7733287506444406 >10 0.6199212532462093 7.53279486738844 >50 0.03350925693222753 1.4378186400870712 >100 0.016754628466113766 1.2430543621469896 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.6530331672108609 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 103 0.5900211949361287 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 91 0.5212808615455119 No Hit GTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAGCA 57 0.32651658360543045 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 52 0.29787477802600676 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 51 0.292146416910122 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.28068969467835253 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.2635046113306983 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41 0.23486280575127455 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.22340608351950506 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.22340608351950506 No Hit GAGTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAG 30 0.17185083347654237 No Hit GCTTTAGGGGTGCAAATGGTTTTTGGTTACATGGATGCATTGTATAGTAG 29 0.1661224723606576 No Hit GTGATATCCCCTTTATCATTTTTCATTGCGTCTATTTGATTCTTCTCTCT 29 0.1661224723606576 No Hit GTGTTTATAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATCGGTGG 27 0.1546657501288881 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.14320902789711865 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 24 0.13748066678123388 No Hit GTATAGTAGATGCATCTGGGCTTTCATTGTACCCCTCACCTGAATAGTGT 24 0.13748066678123388 No Hit GTATGGGAGAAGGGAGAGCCGGACCCTGGATCCAGAAGAAATGTGACTGT 24 0.13748066678123388 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.13175230566534915 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23 0.13175230566534915 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 23 0.13175230566534915 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.13175230566534915 No Hit GTACATGGGACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGT 22 0.1260239445494644 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.12029558343357966 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21 0.12029558343357966 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.11456722231769491 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20 0.11456722231769491 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 19 0.10883886120181016 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19 0.10883886120181016 No Hit AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.10311050008592541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.005728361115884746 0.0 16-17 0.0 0.0 0.0 0.011456722231769492 0.0 18-19 0.0 0.0 0.0 0.017185083347654236 0.0 20-21 0.0 0.0 0.0 0.025777625021481357 0.0 22-23 0.0 0.0 0.0 0.042962708369135585 0.0 24-25 0.0 0.0 0.0 0.0630119722747322 0.0 26-27 0.0 0.0 0.0 0.08019705562238644 0.0 28-29 0.0 0.0 0.0 0.09738213897004067 0.0 30-31 0.0 0.0 0.0 0.12315976399152202 0.0 32-33 0.0 0.0 0.0 0.18044337515036946 0.0 34-35 0.0 0.0 0.0 0.24918370854098643 0.0 36-37 0.0 0.0 0.0 0.32651658360543045 0.0 38-39 0.0 0.0 0.0 0.461133069828722 0.0 40-41 0.0 0.0 0.0 0.6530331672108609 0.0 42-43 0.0 0.0 0.0 0.7790571117603253 0.0 44-45 0.0 0.0 0.0 0.9079452368677321 0.0 46-47 0.0 0.0 0.0 1.0654751675545626 0.0 48-49 0.0 0.0 0.0 1.2058200148937388 0.0 50-51 0.0 0.0 0.0 1.3776708483702813 0.0 52-53 0.0 0.0 0.0 1.5036947929197457 0.0 54-55 0.0 0.0 0.0 1.675545626396288 0.0 56-57 0.0 0.0 0.0 1.9161367932634472 0.0 58-59 0.0 0.0 0.0 2.067938362834393 0.0 60-61 0.0 0.0 0.0 2.2455175574268202 0.0 62-63 0.0 0.0 0.0 2.388726585323939 0.0 64-65 0.0 0.0 0.0 2.58349086326402 0.0 66-67 0.0 0.0 0.0 2.743884974508793 0.0 68-69 0.0 0.0 0.0 2.867044738500315 0.0 70-71 0.0 0.0 0.0 3.0589448358824542 0.0 72-73 0.0 0.0 0.0 3.3310419888869793 0.0 74-75 0.0 0.0 0.0 3.591682419659735 0.0 76-77 0.0 0.0 0.0 3.783582517041874 0.0 78-79 0.0 0.0 0.0 3.9554333505184167 0.0 80-81 0.0 0.0 0.0 4.244715586870596 0.0 82-83 0.0 0.0 0.0 4.571232170476026 0.0 84-85 0.0 0.0 0.0 4.800366615111416 0.0 86-87 0.0 0.0 0.0 5.144068282064501 0.0 88-89 0.0 0.0 0.0 5.562238643524088 0.0 90-91 0.0 0.0 0.0 5.986137366099559 0.0 92-93 0.0 0.0 0.0 6.36134501919001 0.0 94-95 0.0 0.0 0.0 6.768058658417827 0.0 96-97 0.0 0.0 0.0 7.386721658933379 0.0 98-99 0.0 0.0 0.0 7.819212923182677 0.0 100-101 0.0 0.0 0.0 8.294666895801111 0.0 102-103 0.0 0.0 0.0 8.621183479406541 0.0 104-105 0.0 0.0 0.0 9.045082201982012 0.0 106-107 0.0 0.0 0.0 9.40310477172481 0.0 108-109 0.0 0.0 0.0 9.832731855416165 0.0 110-111 0.0 0.0 0.0 10.351148536403734 0.0 112-113 0.0 0.0 0.0 10.866701036833362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGA 20 5.542215E-6 118.97995 2 TACAAGA 15 2.6585857E-4 118.979935 2 ACATGAA 30 3.4351433E-7 99.14995 3 CATGAAA 25 1.679723E-5 95.18395 4 CAAGAGA 20 8.346828E-4 89.23496 4 GTACAAG 20 8.346828E-4 89.23496 1 GAGAACT 25 0.0020243158 71.38796 9 CAACTAA 25 0.0020243158 71.38796 6 CGCAGAA 50 8.4351996E-8 71.38796 2 ACAAGAG 35 8.9102105E-5 67.98854 3 GCAGAAA 70 1.24364306E-8 59.48997 3 TCATCTC 15 0.0042050686 59.489967 84-85 AGAGAAC 65 5.1798634E-7 54.91382 5 AACAAGA 55 1.2590297E-5 54.081787 2 ATGGGAG 45 3.0876088E-4 52.879974 5 AAGAGAA 45 3.0876088E-4 52.879974 5 ATGAAAA 35 0.0076740766 50.9914 5 GCAGAGA 85 5.750917E-8 48.991745 3 CAGAGAA 75 1.3902772E-6 47.591976 4 AGAACAT 65 3.3822027E-5 45.761517 7 >>END_MODULE