FastQCFastQC Report
Thu 26 May 2016
SRR936223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17457
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5623.219338947127227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4482.566305779916366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3702.119493612877356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.7962421951079796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.584292833820244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT910.5212808615455119No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG830.47545397261843386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA730.4181703614595864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC630.36088675030073897No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG590.3379733058372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC530.30360313914189146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC430.24631952798304405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.23486280575127455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.23486280575127455No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC390.22340608351950506No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT370.21194936128773556No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.20622100017185083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC350.20049263905596607No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG340.19476427794008136No Hit
GAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCTTCAAAAAATCAAT340.19476427794008136No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC330.1890359168241966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC330.1890359168241966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC320.18330755570831186No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG320.18330755570831186No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.18330755570831186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG320.18330755570831186No Hit
GCTTTAGGGGTGCAAATGGTTTTTGGTTACATGGATGCATTGTATAGTAG280.16039411124477287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG280.16039411124477287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG280.16039411124477287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC280.16039411124477287No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14893738901300338No Hit
GCTATATCCTGGATGCTGTCCTGTTTTTGTATGGTATTGTCCTTACCCTA260.14893738901300338No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA250.14320902789711865No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.14320902789711865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC240.13748066678123388No Hit
CTACAGAATGGTATAGTGGACACTGAAGACTCAGAAGCAGGGAGGGTAGG240.13748066678123388No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT230.13175230566534915No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.13175230566534915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.1260239445494644No Hit
ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA220.1260239445494644No Hit
GTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAGCA220.1260239445494644No Hit
GTACATGGGTTTTATCCAATTTTTTATTATTTCCATTAGATATCAAAGGA220.1260239445494644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC220.1260239445494644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG210.12029558343357966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC210.12029558343357966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC200.11456722231769491No Hit
GGATGATCTCAGCCGTGATCTTGTTCTTGCTCCTTTTGGTGGAACAAGCA200.11456722231769491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.11456722231769491No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT200.11456722231769491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA200.11456722231769491No Hit
GTGTTTATAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATCGGTGG200.11456722231769491No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200.11456722231769491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT190.10883886120181016No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG190.10883886120181016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA190.10883886120181016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT180.10311050008592541No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT180.10311050008592541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT152.6568025E-4119.01
GTTATCA251.6783179E-595.21
TTATCAA304.147493E-579.3333362
GGTATCA3300.063.1060561
TAACCAT150.00420226259.584-85
CTTCCCT302.1495682E-549.58333262-63
GTATCAA4800.043.3854182
GGCCCCT300.001291165839.66666896-97
TATCAAC6550.035.4274833
AACGCAG6750.035.259267
CAACGCA6750.035.259266
ATCAACG6600.035.159094
TCAACGC6700.034.6343275
ACGCAGA7300.032.6027378
CGCAGAG7300.032.6027379
GAGTACT5950.028.512-13
CAGAGTA7450.027.9530210-11
GTCTTCT450.0094053126.444443118-119
AGTACTT6000.026.2791712-13
ACTTTTT6950.025.25539616-17
Produced by FastQC (version 0.11.5)