##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936223_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17457 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23010826602509 31.0 31.0 33.0 30.0 34.0 2 30.90903362547975 31.0 31.0 34.0 28.0 34.0 3 31.130033797330583 31.0 31.0 34.0 28.0 34.0 4 35.18009967348342 35.0 35.0 37.0 33.0 37.0 5 34.88365698573638 35.0 35.0 37.0 32.0 37.0 6 35.14452655095377 37.0 35.0 37.0 32.0 37.0 7 34.88165205934582 36.0 35.0 37.0 32.0 37.0 8 35.08861774646274 37.0 35.0 37.0 32.0 37.0 9 36.52431689293693 39.0 35.0 39.0 32.0 39.0 10-11 36.43289224952741 39.0 35.0 39.0 32.0 39.0 12-13 36.38546141948788 39.0 35.0 39.0 31.5 39.0 14-15 37.02606404307727 39.0 36.0 41.0 31.0 41.0 16-17 37.012258692787995 38.5 36.0 40.5 31.5 41.0 18-19 37.559832731855415 39.5 36.5 41.0 32.0 41.0 20-21 37.72919172824655 40.0 37.0 41.0 32.0 41.0 22-23 37.58251704187432 40.0 36.0 41.0 32.0 41.0 24-25 37.40591166867159 40.0 36.0 41.0 32.0 41.0 26-27 36.91499112104027 39.0 35.0 41.0 31.0 41.0 28-29 36.54582688892708 39.0 35.0 40.5 30.5 41.0 30-31 35.67508735750702 37.5 35.0 40.0 30.0 41.0 32-33 34.65208798762674 35.5 33.5 40.0 29.0 41.0 34-35 33.86352179641405 35.0 33.0 40.0 25.5 41.0 36-37 33.11946497107178 35.0 33.0 40.0 20.5 41.0 38-39 32.59649424299708 35.0 33.0 40.0 12.5 41.0 40-41 31.819613908460788 35.0 33.0 40.0 2.0 41.0 42-43 31.002319986251933 35.0 31.5 40.0 2.0 41.0 44-45 29.763046342441427 35.0 28.5 40.0 2.0 41.0 46-47 27.918657272154437 35.0 14.0 40.0 2.0 41.0 48-49 26.308042619006702 35.0 2.0 40.0 2.0 41.0 50-51 25.274588990089935 34.5 2.0 40.0 2.0 41.0 52-53 24.8310133470814 34.0 2.0 39.0 2.0 41.0 54-55 24.45082201982013 34.0 2.0 39.0 2.0 41.0 56-57 24.135819442057628 34.0 2.0 39.0 2.0 41.0 58-59 23.864180557942372 33.0 2.0 39.0 2.0 41.0 60-61 23.54757403906742 33.0 2.0 38.5 2.0 41.0 62-63 23.276135647591225 33.0 2.0 38.0 2.0 40.5 64-65 23.01346164862233 33.0 2.0 37.5 2.0 40.0 66-67 22.721028813656414 32.0 2.0 37.0 2.0 40.0 68-69 22.44643982356648 32.0 2.0 37.0 2.0 40.0 70-71 22.124019018158904 31.0 2.0 36.0 2.0 39.5 72-73 21.855215672796014 31.0 2.0 36.0 2.0 39.0 74-75 21.613049206621987 31.0 2.0 35.0 2.0 39.0 76-77 21.283754367875353 31.0 2.0 35.0 2.0 38.0 78-79 21.00234862805751 30.5 2.0 35.0 2.0 37.0 80-81 20.786074354127287 30.0 2.0 35.0 2.0 37.0 82-83 20.566305779916366 30.0 2.0 35.0 2.0 36.0 84-85 20.379475282121785 30.0 2.0 35.0 2.0 36.0 86-87 20.235091940195908 30.0 2.0 35.0 2.0 35.5 88-89 20.116371656069198 29.5 2.0 35.0 2.0 35.0 90-91 20.01844532279315 29.0 2.0 35.0 2.0 35.0 92-93 19.885776479349257 29.0 2.0 35.0 2.0 35.0 94-95 19.809388783868933 29.0 2.0 35.0 2.0 35.0 96-97 19.728217906856848 29.0 2.0 35.0 2.0 35.0 98-99 19.635819442057624 29.0 2.0 35.0 2.0 35.0 100-101 19.55559374462966 29.0 2.0 35.0 2.0 35.0 102-103 18.82408203013118 26.0 2.0 34.0 2.0 35.0 104-105 19.323938821103283 28.5 2.0 34.5 2.0 35.0 106-107 19.383170075041534 29.0 2.0 35.0 2.0 35.0 108-109 19.333562467777966 29.0 2.0 35.0 2.0 35.0 110-111 19.268144583834566 29.0 2.0 35.0 2.0 35.0 112-113 19.19275935154952 29.0 2.0 35.0 2.0 35.0 114-115 19.151257375264937 29.0 2.0 35.0 2.0 35.0 116-117 19.010368333619752 29.0 2.0 35.0 2.0 35.0 118-119 18.850289282236353 27.0 2.0 35.0 2.0 35.0 120-121 18.689637394741364 27.0 2.0 35.0 2.0 35.0 122-123 18.429884859941573 25.0 2.0 35.0 2.0 35.0 124-125 16.937761356475914 13.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.17466959275057903 1101 2 1.0245264357224784 1101 3 0.8873090320795427 1101 4 0.4272270357597492 1101 5 0.9973664695705793 1101 6 0.6948125456778769 1101 7 1.008812951403165 1101 8 1.2796484272817636 1101 9 1.773559367429776 1101 10-11 1.464299184161753 1101 12-13 0.5451796234429196 1101 14-15 1.1334787639111354 1101 16-17 0.342858178075204 1101 18-19 0.8140179673830588 1101 20-21 1.008770210355884 1101 22-23 0.41510525476613225 1101 24-25 0.9143544766977811 1101 26-27 0.25440981630390525 1101 28-29 0.6689027975817794 1101 30-31 -0.8762533943669482 1101 32-33 -1.1478429564042543 1101 34-35 -0.6467627104181446 1101 36-37 0.38674706878286713 1101 38-39 -1.5386287667898237 1101 40-41 -2.0563816501250543 1101 42-43 -1.9035797763134035 1101 44-45 -1.4162189363431814 1101 46-47 -1.6258792771674493 1101 48-49 -1.640230029133832 1101 50-51 -3.3508163152550132 1101 52-53 -3.0627167240161413 1101 54-55 -3.076744935875343 1101 56-57 -2.7655413960430586 1101 58-59 -1.9937581881504052 1101 60-61 -3.151387049273307 1101 62-63 -2.8379860902688527 1101 64-65 -3.3335209764995923 1101 66-67 -3.594852048578886 1101 68-69 -3.195571328530807 1101 70-71 -2.3079481841605833 1101 72-73 -2.1926292486506114 1101 74-75 -1.7498314691944614 1101 76-77 -1.2409339546211662 1101 78-79 -1.1274783598732867 1101 80-81 -1.4741704062406562 1101 82-83 -1.3767480408064472 1101 84-85 -1.1939614644355068 1101 86-87 -1.5885981098836552 1101 88-89 -1.2003909631782292 1101 90-91 -1.3717644847866026 1101 92-93 -1.2490207645463514 1101 94-95 -0.9367472582335736 1101 96-97 -0.9913389878574854 1101 98-99 -1.0052744742086297 1101 100-101 -0.8409458417083933 1101 102-103 -1.0729314337820774 1101 104-105 -1.064943124277935 1101 106-107 -1.0908741806394744 1101 108-109 -0.9575908161295033 1101 110-111 -1.2554797329857017 1101 112-113 -0.5325028498465514 1101 114-115 -0.6620965299779336 1101 116-117 -0.6689643644377057 1101 118-119 -0.2323715328689211 1101 120-121 -0.5071539278701458 1101 122-123 -1.9496343629304533 1101 124-125 -1.9544871873178167 1102 1 -0.4259923440733324 1102 2 0.7083888695849119 1102 3 0.2841344289049381 1102 4 0.7288143373470461 1102 5 -0.20898273677862989 1102 6 -0.4070393061739779 1102 7 0.26278120537142513 1102 8 0.37356377119710515 1102 9 -0.7343771405067301 1102 10-11 0.3234261682887407 1102 12-13 0.04650237476566588 1102 14-15 0.6982935787259521 1102 16-17 -0.5638878536708205 1102 18-19 -1.0768550484899535 1102 20-21 -0.6790604774748061 1102 22-23 0.1108724505333285 1102 24-25 0.2278465401898444 1102 26-27 0.7517643136583985 1102 28-29 0.46586046953945726 1102 30-31 -0.7267824948960424 1102 32-33 -0.16570009926139306 1102 34-35 1.0628933742379374 1102 36-37 1.0560592380950382 1102 38-39 1.6260537728927176 1102 40-41 2.8364754927320845 1102 42-43 1.2055472078135843 1102 44-45 2.6380138678896223 1102 46-47 4.742506966218798 1102 48-49 5.597203833300032 1102 50-51 5.846273631834936 1102 52-53 6.15487586857645 1102 54-55 5.934498450368043 1102 56-57 6.336310455808793 1102 58-59 6.6100777906855726 1102 60-61 6.291073268187013 1102 62-63 5.997331370048606 1102 64-65 6.923754155775541 1102 66-67 7.3416558879290506 1102 68-69 7.6781059201464394 1102 70-71 7.336893085680689 1102 72-73 7.3179527619314015 1102 74-75 7.78852831916533 1102 76-77 7.179700966013755 1102 78-79 6.905590423195495 1102 80-81 6.59659678952654 1102 82-83 6.358040318981914 1102 84-85 7.1307739852999426 1102 86-87 5.8624600911745475 1102 88-89 6.492069354282091 1102 90-91 6.7757222877001695 1102 92-93 6.612751722226136 1102 94-95 5.2034643819780655 1102 96-97 5.904163657645164 1102 98-99 6.283085314151162 1102 100-101 6.961302835540284 1102 102-103 6.173100312249666 1102 104-105 7.327914018579211 1102 106-107 7.082406242640946 1102 108-109 7.121774263235576 1102 110-111 7.087774235268263 1102 112-113 6.911280218936518 1102 114-115 6.824014581133177 1102 116-117 7.167014471541126 1102 118-119 6.430326879829494 1102 120-121 6.7587190880028665 1102 122-123 6.534492621196534 1102 124-125 6.263105405274775 1103 1 0.5124313012468669 1103 2 0.6899159631809724 1103 3 0.8604891087078954 1103 4 0.4578783767559145 1103 5 0.7023473124824591 1103 6 0.39468483175705416 1103 7 -0.4662828340374432 1103 8 0.32307116035966743 1103 9 0.37892335210410977 1103 10-11 0.4336478431655806 1103 12-13 0.46152700530754487 1103 14-15 -0.15515469713611907 1103 16-17 -0.04603071081368171 1103 18-19 -0.18215061499241614 1103 20-21 -0.6470407730413044 1103 22-23 -1.086171884442038 1103 24-25 -0.9457987800072019 1103 26-27 -0.6644918439770677 1103 28-29 -0.36110997381030074 1103 30-31 -0.3462406229748609 1103 32-33 -1.3202567495076991 1103 34-35 -1.793633719358123 1103 36-37 -2.5794087422005276 1103 38-39 -0.375793517747681 1103 40-41 0.5298252464266753 1103 42-43 -0.5204380138612947 1103 44-45 1.774713375278786 1103 46-47 2.9130734686818514 1103 48-49 2.4561939810832847 1103 50-51 3.3056332377462674 1103 52-53 2.788496558231625 1103 54-55 4.169104361698093 1103 56-57 2.7242414902915506 1103 58-59 2.275718184774245 1103 60-61 2.7202604603052407 1103 62-63 3.3957303848269333 1103 64-65 3.6288034168592844 1103 66-67 3.7135770701950577 1103 68-69 2.9398054275355996 1103 70-71 2.264848750705319 1103 72-73 2.931253254542238 1103 74-75 2.7207943290175436 1103 76-77 2.002361064535922 1103 78-79 2.1605165315698756 1103 80-81 2.7831731442063408 1103 82-83 3.2835329298193514 1103 84-85 3.8813897488467397 1103 86-87 3.799652209401149 1103 88-89 4.050566891227902 1103 90-91 3.8440720413947496 1103 92-93 2.225436451290175 1103 94-95 3.471937288382005 1103 96-97 3.3413557758717616 1103 98-99 3.6894892550378593 1103 100-101 3.9151205695304334 1103 102-103 2.9883712482102602 1103 104-105 4.210918944687581 1103 106-107 3.2437362919020316 1103 108-109 3.3163555440237538 1103 110-111 3.6142520677805834 1103 112-113 4.238250662286273 1103 114-115 3.311083546650419 1103 116-117 3.4280982153834927 1103 118-119 3.2024943675142232 1103 120-121 3.075221551057055 1103 122-123 3.330059123659588 1103 124-125 3.1036226466540846 1104 1 -0.1376325557135445 1104 2 0.7282301394261843 1104 3 0.10953125430176414 1104 4 0.019819628352337304 1104 5 -0.4470779748738636 1104 6 0.2503681012334269 1104 7 0.6754796180698364 1104 8 -0.016647869014533967 1104 9 0.6994852933556999 1104 10-11 -0.9245897047271328 1104 12-13 -0.8992648210015233 1104 14-15 -0.9591138286814527 1104 16-17 0.324339659556685 1104 18-19 -1.8156116622465674 1104 20-21 -1.398637197051528 1104 22-23 -1.5848947452338678 1104 24-25 -1.3449047825614784 1104 26-27 -0.6159605540664614 1104 28-29 -0.4607268320478468 1104 30-31 -1.4873645054780553 1104 32-33 0.3891940806327838 1104 34-35 -0.4800960437514874 1104 36-37 -0.8725121904763924 1104 38-39 -0.7238139519750106 1104 40-41 -2.537863131606535 1104 42-43 -2.5146908874245106 1104 44-45 -1.1199226400468838 1104 46-47 -1.551805203093373 1104 48-49 -2.251341140244943 1104 50-51 -3.017482981921681 1104 52-53 -2.4516056129050305 1104 54-55 -1.539707898838305 1104 56-57 -1.4507265812282455 1104 58-59 -1.7530174474096611 1104 60-61 -1.9106463085325665 1104 62-63 -1.8009490532318182 1104 64-65 -0.9816691246477411 1104 66-67 -1.3541113078381457 1104 68-69 -1.4733491063085857 1104 70-71 -0.9005407767531715 1104 72-73 -0.785221841243203 1104 74-75 -0.6757573951203852 1104 76-77 -0.4631561768433876 1104 78-79 -0.07192280431773312 1104 80-81 -0.3075037395739919 1104 82-83 -0.9137850778434817 1104 84-85 -0.15692442739847223 1104 86-87 0.09658707530153166 1104 88-89 -0.330020592807859 1104 90-91 -0.27917189219401095 1104 92-93 -0.06383557936116446 1104 94-95 -0.36267318415950456 1104 96-97 -0.38022787674637115 1104 98-99 -0.3756448445789964 1104 100-101 -0.17427917504172896 1104 102-103 0.16780930695866303 1104 104-105 0.0091309497961376 1104 106-107 -0.016800106565401762 1104 108-109 -0.1612945198332092 1104 110-111 0.2815573040513364 1104 112-113 0.37490455756085694 1104 114-115 0.89345902557762 1104 116-117 0.5347393392659967 1104 118-119 0.10096180046441461 1104 120-121 0.10395718324096137 1104 122-123 0.38369897040288237 1104 124-125 0.4344017015710726 1105 1 0.2882933702123829 1105 2 0.018828430024473874 1105 3 0.42149706626757677 1105 4 0.21782532635803875 1105 5 0.027280999485107316 1105 6 -0.9262700754047444 1105 7 -1.6877682451780274 1105 8 0.33235497998830965 1105 9 -0.6025090086385987 1105 10-11 -0.5378375679749965 1105 12-13 -0.16778333952004232 1105 14-15 0.1790628094951856 1105 16-17 -0.1806460954290685 1105 18-19 0.7734196767847692 1105 20-21 -0.11587366428798873 1105 22-23 0.1465867362476132 1105 24-25 -0.8847908224475205 1105 26-27 -0.6273565654624775 1105 28-29 0.5414099200889027 1105 30-31 -0.5372220553356044 1105 32-33 -2.6863044948657944 1105 34-35 -3.295623109278548 1105 36-37 -3.737896805861009 1105 38-39 -3.6198253479864064 1105 40-41 -6.114073957817361 1105 42-43 -8.386485759219386 1105 44-45 -7.416931187055429 1105 46-47 -8.080295231583403 1105 48-49 -8.3574664963703 1105 50-51 -7.4861439505826475 1105 52-53 -7.373970285269703 1105 54-55 -8.011930121060526 1105 56-57 -8.009843390345054 1105 58-59 -8.067120011512227 1105 60-61 -7.320190468076724 1105 62-63 -7.295251047533808 1105 64-65 -7.418278811257428 1105 66-67 -6.2187836725105115 1105 68-69 -6.232608365567845 1105 70-71 -5.790854167066563 1105 72-73 -6.088640644662005 1105 74-75 -5.694988164351155 1105 76-77 -6.117002330689541 1105 78-79 -6.393860126255056 1105 80-81 -5.954227386297637 1105 82-83 -5.206520120578524 1105 84-85 -5.351368871842915 1105 86-87 -5.305122326407872 1105 88-89 -4.83073284352011 1105 90-91 -5.011365624387743 1105 92-93 -4.561698827224415 1105 94-95 -4.49159056307688 1105 96-97 -4.340341836860333 1105 98-99 -4.4119696309037835 1105 100-101 -4.031829032591585 1105 102-103 -6.068657929508573 1105 104-105 -5.411096970431782 1105 106-107 -4.623637713403006 1105 108-109 -4.4276762862149734 1105 110-111 -4.644368621874591 1105 112-113 -4.741192308536007 1105 114-115 -3.8779084957898995 1105 116-117 -3.761556957030301 1105 118-119 -3.926815977313364 1105 120-121 -3.4665556372718562 1105 122-123 -3.394078807374896 1105 124-125 -5.534971517802148 1106 1 0.24983183175084278 1106 2 -0.17347926228321953 1106 3 0.5753432201137301 1106 4 0.7178253263580388 1106 5 0.7580502302543408 1106 6 -0.6185777677124378 1106 7 -0.4185374759472609 1106 8 -0.013798866165529944 1106 9 -0.4102013163309053 1106 10-11 -0.01860679874422999 1106 12-13 -0.9177833395200423 1106 14-15 -1.417091036658661 1106 16-17 -0.4691076338906015 1106 18-19 -1.053503400138304 1106 20-21 -0.7889505873649085 1106 22-23 -0.930336340675467 1106 24-25 -1.3078677455244403 1106 26-27 0.19956651146060267 1106 28-29 -0.766282387603404 1106 30-31 -1.133375901489451 1106 32-33 -2.070919879481174 1106 34-35 -1.6802384938939312 1106 36-37 -0.9494352673994655 1106 38-39 -1.677517655678713 1106 40-41 -2.5756124193558243 1106 42-43 -3.0211011438347697 1106 44-45 -3.1669311870554324 1106 46-47 -5.061064462352633 1106 48-49 -5.703620342524143 1106 50-51 -6.697682412121107 1106 52-53 -6.662431823731243 1106 54-55 -6.5119301210605265 1106 56-57 -6.221381851883514 1106 58-59 -5.6825046268968435 1106 60-61 -5.4740366219228775 1106 62-63 -5.660635662918423 1106 64-65 -6.1105865035651235 1106 66-67 -5.122629826356665 1106 68-69 -4.424916057875539 1106 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1.280056245708053 2216 18-19 0.5000083055473112 2216 20-21 0.5538587771835779 2216 22-23 1.5270215188563085 2216 24-25 0.8443061675190364 2216 26-27 0.6585966117950477 2216 28-29 0.33154369935311223 2216 30-31 1.0915404864704144 2216 32-33 -0.06088643466512167 2216 34-35 -0.6894358183086453 2216 36-37 -2.3072947991720447 2216 38-39 -0.7970828730700177 2216 40-41 -0.36072947621201124 2216 42-43 3.0867583879378095 2216 44-45 0.6675169734796853 2216 46-47 0.558500755038672 2216 48-49 1.0079181190143167 2216 50-51 0.45031089891568143 2216 52-53 -0.31795021838007287 2216 54-55 -2.1749735993213974 2216 56-57 -2.8049938920172934 2216 58-59 -0.6733073024821259 2216 60-61 -1.392932942993113 2216 62-63 -1.0577928535538774 2216 64-65 -1.3037303162741516 2216 66-67 -1.6334993915740554 2216 68-69 -1.2326083655678453 2216 70-71 -0.6654361068658936 2216 72-73 -0.9180720827890951 2216 74-75 -1.219235655989948 2216 76-77 -0.9414170464086062 2216 78-79 -1.4406828687299722 2216 80-81 -1.8839932725852613 2216 82-83 -1.607021792819328 2216 84-85 -2.101368871842915 2216 86-87 -1.3720119585148929 2216 88-89 -2.3984585960284726 2216 90-91 -1.2638739855917542 2216 92-93 -1.662869395786288 2216 94-95 -1.6195169844815638 2216 96-97 -2.1129170876964523 2216 98-99 -2.3225047479606395 2216 100-101 -2.7040698352671697 2216 102-103 -2.140564284023622 2216 104-105 -2.0432039938431537 2216 106-107 -1.4781527635702325 2216 108-109 -0.7136294634725004 2216 110-111 -1.4052381870919817 2216 112-113 -2.1500551847901868 2216 114-115 -1.9205506362581275 2216 116-117 -2.00068739181291 2216 118-119 -3.089859455574233 2216 120-121 -3.171405135599617 2216 122-123 -2.9375570682444625 2216 124-125 -2.644503290377399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 10.0 5 13.0 6 45.0 7 107.0 8 155.0 9 264.0 10 431.0 11 604.0 12 819.0 13 1014.0 14 1329.0 15 1058.0 16 325.0 17 214.0 18 170.0 19 184.0 20 155.0 21 139.0 22 136.0 23 116.0 24 138.0 25 143.0 26 150.0 27 220.0 28 270.0 29 338.0 30 369.0 31 416.0 32 567.0 33 647.0 34 881.0 35 1271.0 36 1663.0 37 2299.0 38 785.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.11550587560906 16.612209802235597 15.070220693608483 15.202063628546862 2 21.01735693418113 25.124591854270495 33.298963166638025 20.55908804491035 3 19.30457696053159 28.53296671822192 37.251532336598494 14.910923984647992 4 11.382253537262988 20.513261155983272 31.55181302629318 36.552672280460555 5 8.415983489078714 42.56721894169581 34.36335492747807 14.653442641747406 6 25.416738271180616 38.91275706020508 14.154780317351207 21.515724351263106 7 16.18834851349029 41.221286589906626 22.913444463538983 19.676920433064097 8 30.37177063642092 31.454430887323138 18.38231082087415 19.791487655381797 9 24.72360657615856 9.829867674858223 21.17202268431002 44.2745030646732 10-11 24.86967978461362 26.6053732027267 25.035802256974282 23.4891447556854 12-13 29.206049149338376 28.33533825972389 29.469553760669072 12.98905883026866 14-15 23.10534456092112 26.45070745259781 35.64186286303489 14.802085123446181 16-17 14.464111817608982 26.15856103568769 37.95898493441027 21.418342212293062 18-19 13.665005441943059 18.342212293062953 50.254912069656875 17.737870195337113 20-21 14.962479234690953 16.981726528040326 54.164518531248206 13.891275706020508 22-23 14.186286303488572 17.700635848083863 54.07286475339406 14.04021309503351 24-25 13.86836226155697 17.271008764392505 54.296270836913564 14.564358137136965 26-27 13.464512802887093 17.1421206392851 54.89488457352352 14.49848198430429 28-29 14.200607206278285 16.904393652975884 55.32737583777282 13.56762330297302 30-31 14.641691012201411 16.933035458555306 54.373603711978 14.05166981726528 32-33 13.876954803230795 17.399896889499914 54.39078879532566 14.332359511943633 34-35 14.289396803574498 17.351205820014894 53.71197800309332 14.647419373317295 36-37 14.108953428424128 17.545970097954974 52.572034141032255 15.773042332588647 38-39 15.724351263103625 17.39703270894197 50.988142292490124 15.890473735464283 40-41 15.956349888296959 18.30211376525176 50.146073208455064 15.595463137996218 42-43 16.60842165568605 18.461758808364365 48.647951876253224 16.28186765969636 44-45 16.81466628473217 19.415640217702666 45.72328845602979 18.046405041535376 46-47 17.33887138355772 19.699226582641078 42.70982526496706 20.252076768834147 48-49 19.24663420223432 20.807791463763962 39.02033801203093 20.925236321970782 50-51 19.693497565167572 21.25179031796047 37.467774276711545 21.58693784016041 52-53 20.080206244629046 22.085362360355198 35.56574047550845 22.268690919507307 54-55 18.65654540246348 23.526210254941276 34.53451733027786 23.282727012317388 56-57 18.33338107651322 22.75344467042883 33.54722277922599 25.365951473831966 58-59 18.45316528215411 22.663993125179033 33.05929533085076 25.823546261816098 60-61 19.125229147571034 22.08123281393217 33.656049495875344 25.13748854262145 62-63 16.970269805808556 22.03127685169273 35.833762960417026 25.164690382081687 64-65 17.937039901463724 21.22826616252757 35.17229526510269 25.66239867090602 66-67 17.816223648029332 21.59429422548121 34.8332951420715 25.756186984417965 68-69 18.78723648029331 21.156049495875344 35.45772227314391 24.598991750687443 70-71 19.028386468448343 21.211079602417577 35.40717825327261 24.353355675861476 72-73 19.243769693497565 21.297622457748496 36.230306502434836 23.228301346319107 74-75 18.987740604949586 21.28494500458295 35.55224564619615 24.17506874427131 76-77 18.605716904393653 21.724809531992896 36.183192988486 23.486280575127456 78-79 19.021023085295298 21.444119837314542 35.54734490462278 23.98751217276737 80-81 18.161768917912585 21.54723033740047 35.54734490462278 24.743655840064157 82-83 17.302514750529873 22.237497851864582 35.28384029329209 25.176147104313458 84-85 17.554562639628802 22.884802657959558 33.07269290256058 26.48794179985106 86-87 17.97559718164633 22.810333963453054 32.2363521796414 26.977716675259206 88-89 16.667144043765934 22.62481024260304 32.326067653882504 28.38197805974852 90-91 16.746863722289053 22.735865268946554 31.465887609554905 29.051383399209485 92-93 16.689580111130205 23.065246033109926 29.79034198315862 30.45483187260125 94-95 16.643753222203127 23.7326001031105 28.53296671822192 31.090679956464456 96-97 17.91544938992954 23.44331786675832 27.103740619808676 31.537492123503462 98-99 18.131301558203482 22.09269019248396 27.11102199816682 32.66498625114574 100-101 17.984189723320156 22.449447213152318 26.731397147276166 32.83496591625136 102-103 17.78942544538008 23.60944033911898 25.720341410322504 32.88079280517844 104-105 18.39143012631398 23.867900209091168 25.451837424455075 32.288832240139776 106-107 17.91544938992954 24.159363006243915 25.416738271180616 32.50844933264593 108-109 17.872486681560407 23.904450936587043 25.78335338259724 32.43970899925531 110-111 18.717956062211783 24.177240569415403 26.096296508463894 31.00850685990892 112-113 18.576871293935664 23.452404823970895 26.769600962502505 31.201122919590933 114-115 17.91955537729903 23.683607402738787 26.834928092591532 31.56190912737065 116-117 18.42376668767547 23.468744628430642 27.084168910788975 31.023319773104912 118-119 18.54589205912685 22.923112180588976 28.789962186318323 29.741033573965854 120-121 18.040676024061874 23.394442853050702 28.85419650529934 29.710684617588086 122-123 17.144575897333066 23.727405540118593 28.986794236443323 30.141224326105014 124-125 17.02377542251504 23.993125179031797 28.596390718991692 30.38670867946147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 4.0 3 7.0 4 4.0 5 5.0 6 10.0 7 20.5 8 38.5 9 53.0 10 52.5 11 52.0 12 60.5 13 57.5 14 55.5 15 60.5 16 59.0 17 52.5 18 66.5 19 83.0 20 83.0 21 102.0 22 119.5 23 134.5 24 164.5 25 201.5 26 229.0 27 285.5 28 347.5 29 383.5 30 443.5 31 500.5 32 550.0 33 609.5 34 671.5 35 699.0 36 701.0 37 657.5 38 589.5 39 530.5 40 460.5 41 433.0 42 438.5 43 417.0 44 366.0 45 358.0 46 373.5 47 380.5 48 421.5 49 520.0 50 723.5 51 678.0 52 462.0 53 373.0 54 299.0 55 267.5 56 225.0 57 193.0 58 196.5 59 211.5 60 176.0 61 106.0 62 87.0 63 83.5 64 71.0 65 64.5 66 59.5 67 54.0 68 37.0 69 25.0 70 31.0 71 25.0 72 18.5 73 17.5 74 15.0 75 14.0 76 7.5 77 3.5 78 8.5 79 8.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06874033339061694 2 0.0 3 0.0 4 0.0 5 0.08019705562238644 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011456722231769492 44-45 0.011456722231769492 46-47 0.011456722231769492 48-49 0.011456722231769492 50-51 0.011456722231769492 52-53 0.011456722231769492 54-55 0.011456722231769492 56-57 0.014320902789711864 58-59 0.011456722231769492 60-61 0.005728361115884746 62-63 0.0 64-65 0.008592541673827118 66-67 0.005728361115884746 68-69 0.005728361115884746 70-71 0.008592541673827118 72-73 0.011456722231769492 74-75 0.005728361115884746 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.002864180557942373 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.005728361115884746 100-101 0.0 102-103 0.0 104-105 0.002864180557942373 106-107 0.0 108-109 0.0 110-111 0.002864180557942373 112-113 0.014320902789711864 114-115 0.022913444463538983 116-117 0.022913444463538983 118-119 0.017185083347654236 120-121 0.011456722231769492 122-123 0.014320902789711864 124-125 0.011456722231769492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 17457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.54133012545111 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0991726170237 37.956120753852325 2 10.223280063470474 10.33396345305608 3 5.961691034795421 9.039353840866129 4 3.1961917715062906 6.461591338717993 5 1.9834523404737618 5.012315976399153 6 0.929389096679134 2.818353669015295 7 0.5553666553326533 1.9648278627484677 8 0.3853564547206166 1.5581142235206509 9 0.260682307605123 1.185770750988142 >10 1.2807435112773433 11.966546371083234 >50 0.06800408024481469 2.4173683909033623 >100 0.04533605349654313 6.066334421721945 >500 0.011334013374135782 3.219338947127227 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 3.219338947127227 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 448 2.566305779916366 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 370 2.119493612877356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 0.7962421951079796 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 102 0.584292833820244 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 91 0.5212808615455119 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 83 0.47545397261843386 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 73 0.4181703614595864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 63 0.36088675030073897 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 59 0.3379733058372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 53 0.30360313914189146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 43 0.24631952798304405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 41 0.23486280575127455 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 41 0.23486280575127455 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 39 0.22340608351950506 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 37 0.21194936128773556 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.20622100017185083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 35 0.20049263905596607 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 34 0.19476427794008136 No Hit GAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCTTCAAAAAATCAAT 34 0.19476427794008136 No Hit ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC 33 0.1890359168241966 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 33 0.1890359168241966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 32 0.18330755570831186 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 32 0.18330755570831186 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.18330755570831186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 32 0.18330755570831186 No Hit GCTTTAGGGGTGCAAATGGTTTTTGGTTACATGGATGCATTGTATAGTAG 28 0.16039411124477287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 28 0.16039411124477287 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 28 0.16039411124477287 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 28 0.16039411124477287 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.14893738901300338 No Hit GCTATATCCTGGATGCTGTCCTGTTTTTGTATGGTATTGTCCTTACCCTA 26 0.14893738901300338 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 25 0.14320902789711865 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.14320902789711865 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 24 0.13748066678123388 No Hit CTACAGAATGGTATAGTGGACACTGAAGACTCAGAAGCAGGGAGGGTAGG 24 0.13748066678123388 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 23 0.13175230566534915 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.13175230566534915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 22 0.1260239445494644 No Hit ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA 22 0.1260239445494644 No Hit GTACATGGGAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGCTAGCA 22 0.1260239445494644 No Hit GTACATGGGTTTTATCCAATTTTTTATTATTTCCATTAGATATCAAAGGA 22 0.1260239445494644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 22 0.1260239445494644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 21 0.12029558343357966 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 21 0.12029558343357966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 20 0.11456722231769491 No Hit GGATGATCTCAGCCGTGATCTTGTTCTTGCTCCTTTTGGTGGAACAAGCA 20 0.11456722231769491 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 20 0.11456722231769491 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 20 0.11456722231769491 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 20 0.11456722231769491 No Hit GTGTTTATAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATCGGTGG 20 0.11456722231769491 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20 0.11456722231769491 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 19 0.10883886120181016 No Hit ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG 19 0.10883886120181016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 19 0.10883886120181016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 18 0.10311050008592541 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 18 0.10311050008592541 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.005728361115884746 0.0 16-17 0.0 0.0 0.0 0.011456722231769492 0.0 18-19 0.0 0.0 0.0 0.017185083347654236 0.0 20-21 0.0 0.0 0.0 0.025777625021481357 0.0 22-23 0.0 0.0 0.0 0.042962708369135585 0.0 24-25 0.0 0.0 0.0 0.0630119722747322 0.0 26-27 0.0 0.0 0.0 0.08019705562238644 0.0 28-29 0.0 0.0 0.0 0.09738213897004067 0.0 30-31 0.0 0.0 0.0 0.12602394454946442 0.0 32-33 0.0 0.0 0.0 0.18044337515036946 0.0 34-35 0.0 0.0 0.0 0.24918370854098643 0.0 36-37 0.0 0.0 0.0 0.32651658360543045 0.0 38-39 0.0 0.0 0.0 0.4639972503866644 0.0 40-41 0.0 0.0 0.0 0.6673540700005728 0.0 42-43 0.0 0.0 0.0 0.8048347367818067 0.0 44-45 0.0 0.0 0.0 0.9308586813312711 0.0 46-47 0.0 0.0 0.0 1.085524431460159 0.0 48-49 0.0 0.0 0.0 1.2344618204731628 0.0 50-51 0.0 0.0 0.0 1.4120410150655898 0.0 52-53 0.0 0.0 0.0 1.538064959615054 0.0 54-55 0.0 0.0 0.0 1.7041874319757118 0.0 56-57 0.0 0.0 0.0 1.950506959958756 0.0 58-59 0.0 0.0 0.0 2.1166294323194137 0.0 60-61 0.0 0.0 0.0 2.285616085238013 0.0 62-63 0.0 0.0 0.0 2.428825113135132 0.0 64-65 0.0 0.0 0.0 2.6493670160966944 0.0 66-67 0.0 0.0 0.0 2.818353669015295 0.0 68-69 0.0 0.0 0.0 3.0102537663974336 0.0 70-71 0.0 0.0 0.0 3.213610586011342 0.0 72-73 0.0 0.0 0.0 3.534398808500888 0.0 74-75 0.0 0.0 0.0 3.8465944893166064 0.0 76-77 0.0 0.0 0.0 4.09291401729965 0.0 78-79 0.0 0.0 0.0 4.287678295239732 0.0 80-81 0.0 0.0 0.0 4.608466517729278 0.0 82-83 0.0 0.0 0.0 4.975081629145901 0.0 84-85 0.0 0.0 0.0 5.212808615455119 0.0 86-87 0.0 0.0 0.0 5.565102824082031 0.0 88-89 0.0 0.0 0.0 6.006186630005155 0.0 90-91 0.0 0.0 0.0 6.450134616486223 0.0 92-93 0.0 0.0 0.0 6.8425273529243285 0.0 94-95 0.0 0.0 0.0 7.263561894941857 0.0 96-97 0.0 0.0 0.0 7.913730881594776 0.0 98-99 0.0 0.0 0.0 8.36340722919173 0.0 100-101 0.0 0.0 0.0 8.878959729621355 0.0 102-103 0.0 0.0 0.0 9.245574841037978 0.0 104-105 0.0 0.0 0.0 9.652288480265796 0.0 106-107 0.0 0.0 0.0 10.030360313914189 0.0 108-109 0.0 0.0 0.0 10.508678467090565 0.0 110-111 0.0 0.0 0.0 11.055736953657558 0.0 112-113 0.0 0.0 0.0 11.642893968035745 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATT 15 2.6568025E-4 119.0 1 GTTATCA 25 1.6783179E-5 95.2 1 TTATCAA 30 4.147493E-5 79.333336 2 GGTATCA 330 0.0 63.106056 1 TAACCAT 15 0.004202262 59.5 84-85 CTTCCCT 30 2.1495682E-5 49.583332 62-63 GTATCAA 480 0.0 43.385418 2 GGCCCCT 30 0.0012911658 39.666668 96-97 TATCAAC 655 0.0 35.427483 3 AACGCAG 675 0.0 35.25926 7 CAACGCA 675 0.0 35.25926 6 ATCAACG 660 0.0 35.15909 4 TCAACGC 670 0.0 34.634327 5 ACGCAGA 730 0.0 32.602737 8 CGCAGAG 730 0.0 32.602737 9 GAGTACT 595 0.0 28.5 12-13 CAGAGTA 745 0.0 27.95302 10-11 GTCTTCT 45 0.00940531 26.444443 118-119 AGTACTT 600 0.0 26.27917 12-13 ACTTTTT 695 0.0 25.255396 16-17 >>END_MODULE