FastQCFastQC Report
Thu 26 May 2016
SRR936222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749895
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.065.6344451
GTACATG150700.052.1508331
TACATGG158000.048.6870162
GTATCAA40450.047.800952
ACATGGG155500.047.3272253
CATGGGA99900.043.7717134
CATGGGG51200.040.9019854
ATGGGAT41850.039.2360535
ATCAACG50350.038.9929544
CAACGCA51500.038.4688076
TCAACGC51150.038.3830955
AACGCAG53150.037.05077
ATGGGAG39750.035.4717755
ATGGGAC19100.034.2634435
TATCAAC60450.032.5764243
TACCTGG15350.032.1692852
GACCGTA750.00391303631.7300267
ATGGGGA25300.030.805095
ACGCAGA64000.030.2116958
GAGTACA92500.029.5861051
Produced by FastQC (version 0.11.5)