##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749895 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.65804062529466 31.0 28.0 31.0 15.0 34.0 2 27.768618688549886 31.0 27.0 31.0 15.0 34.0 3 28.028781726903613 31.0 28.0 33.0 16.0 34.0 4 31.311103237622827 35.0 32.0 37.0 16.0 37.0 5 31.590940027830243 35.0 32.0 37.0 17.0 37.0 6 31.5179962226305 35.0 32.0 37.0 17.0 37.0 7 31.358859817303323 35.0 32.0 37.0 15.0 37.0 8 31.235278116687002 35.0 32.0 37.0 13.0 37.0 9 32.458025767260324 37.0 32.0 39.0 10.0 39.0 10-11 32.281394597961594 37.0 32.0 39.0 10.0 39.0 12-13 32.25089819674895 37.0 32.0 39.0 10.0 39.0 14-15 33.14067244034642 38.0 32.0 40.0 10.0 41.0 16-17 33.12255449612691 38.0 32.0 40.0 10.0 41.0 18-19 33.12912689047057 38.0 32.0 40.0 10.5 41.0 20-21 33.204712568468395 38.0 32.0 40.0 11.5 41.0 22-23 33.08880332819969 38.0 31.5 40.0 11.0 41.0 24-25 33.04153763511525 38.0 31.0 40.0 12.0 41.0 26-27 33.09707868186376 38.0 31.0 40.0 12.0 41.0 28-29 32.96576308864246 38.0 31.0 40.0 10.0 41.0 30-31 32.862174873349545 38.0 31.0 40.0 10.0 41.0 32-33 32.66016446701087 38.0 30.0 40.0 9.0 41.0 34-35 32.60634838090285 38.0 30.0 40.0 9.0 41.0 36-37 32.50295474871349 38.0 30.0 40.0 8.0 41.0 38-39 32.23839544658394 38.0 30.0 40.0 8.0 41.0 40-41 32.310540060975086 37.5 30.0 40.0 7.5 41.0 42-43 32.21371739447224 38.0 30.0 40.0 3.5 41.0 44-45 32.07858842959149 37.0 30.0 40.0 2.0 41.0 46-47 32.01763505810349 37.0 30.0 40.0 2.0 41.0 48-49 31.90625294660537 37.0 30.0 40.0 2.0 41.0 50-51 31.760416196400357 37.0 30.0 40.0 2.0 41.0 52-53 31.57950191297192 37.0 30.0 40.0 2.0 41.0 54-55 31.37941562208018 36.5 30.0 40.0 2.0 41.0 56-57 31.154974727055052 36.0 29.0 40.0 2.0 41.0 58-59 30.90079233325428 36.0 29.0 40.0 2.0 41.0 60-61 30.63899576831753 35.5 29.0 39.0 2.0 41.0 62-63 30.382613528240263 35.0 28.0 39.0 2.0 41.0 64-65 30.082171501718676 35.0 28.0 39.0 2.0 40.0 66-67 29.77584255055303 35.0 28.0 38.5 2.0 40.0 68-69 29.48579657636601 34.0 28.0 38.0 2.0 40.0 70-71 29.15298403618503 34.0 28.0 37.5 2.0 40.0 72-73 28.838973481266017 34.0 27.0 37.0 2.0 39.5 74-75 28.504135962443463 34.0 26.5 36.5 2.0 39.0 76-77 28.188638175433383 34.0 26.0 36.0 2.0 39.0 78-79 27.85654539272356 34.0 26.0 36.0 2.0 38.5 80-81 27.554864148991797 33.0 26.0 35.0 2.0 37.0 82-83 27.262810625780403 33.0 26.0 35.0 2.0 37.0 84-85 27.278283554156104 33.0 26.0 35.0 2.0 36.5 86-87 27.162833484294772 33.0 26.0 35.0 2.0 36.0 88-89 26.988945908183062 33.0 26.0 35.0 2.0 36.0 90-91 26.806226087850987 33.0 25.0 35.0 2.0 35.5 92-93 26.68283096985819 33.0 25.0 35.0 2.0 35.0 94-95 26.55040588149575 33.0 25.0 35.0 2.0 35.0 96-97 26.43690735729858 33.0 25.0 35.0 2.0 35.0 98-99 26.306112366742006 33.0 25.0 35.0 2.0 35.0 100-101 26.171382282936975 33.0 24.0 35.0 2.0 35.0 102-103 25.24832461376254 31.5 21.5 34.0 2.0 35.0 104-105 25.865873380974286 33.0 23.5 35.0 2.0 35.0 106-107 25.977193203020754 33.0 24.0 35.0 2.0 35.0 108-109 25.910411196100338 33.0 23.0 35.0 2.0 35.0 110-111 25.78049454395835 33.0 23.0 35.0 2.0 35.0 112-113 25.72556267661774 33.0 23.0 35.0 2.0 35.0 114-115 25.598095314290287 33.0 20.5 35.0 2.0 35.0 116-117 25.440362421745306 33.0 19.5 35.0 2.0 35.0 118-119 25.200133436577623 33.0 18.5 35.0 2.0 35.0 120-121 24.94083044982699 33.0 12.0 35.0 2.0 35.0 122-123 24.575141365624795 33.0 2.0 35.0 2.0 35.0 124-125 22.61669585889439 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47821718979185235 1101 2 0.4546605913014403 1101 3 0.5410874023216579 1101 4 0.45318617729226673 1101 5 0.5480680815138328 1101 6 0.5447319575005878 1101 7 0.4622268835675598 1101 8 0.6664139263475057 1101 9 0.6507160987733371 1101 10-11 0.8019431225360449 1101 12-13 0.6667298112243216 1101 14-15 0.7608884213350322 1101 16-17 0.6094684520826448 1101 18-19 0.47263808113988404 1101 20-21 0.5509005770170035 1101 22-23 0.6434089683143398 1101 24-25 0.6698596437657542 1101 26-27 0.46862048787686206 1101 28-29 0.61815489565452 1101 30-31 0.6107940668532521 1101 32-33 0.5642655179473834 1101 34-35 0.4892895046920529 1101 36-37 0.4348501912396969 1101 38-39 0.6435835853726104 1101 40-41 0.6220391490498898 1101 42-43 0.6132666385025871 1101 44-45 0.550958616066012 1101 46-47 0.437993833467651 1101 48-49 0.4931464268549277 1101 50-51 0.5394268544084007 1101 52-53 0.4799257095017815 1101 54-55 0.4699336428197398 1101 56-57 0.552508259065533 1101 58-59 0.512473163050565 1101 60-61 0.5689114335325023 1101 62-63 0.6055415713986285 1101 64-65 0.5789396457116744 1101 66-67 0.6768965779787699 1101 68-69 0.5192218258073531 1101 70-71 0.4399804067733193 1101 72-73 0.4504393885468332 1101 74-75 0.5596316719085479 1101 76-77 0.5179188354804474 1101 78-79 0.5859936033133195 1101 80-81 0.5296859427172933 1101 82-83 0.5831638629243976 1101 84-85 0.40759441394161655 1101 86-87 0.4176629283742983 1101 88-89 0.4559982379084744 1101 90-91 0.4659916125977972 1101 92-93 0.48604878755477543 1101 94-95 0.5984688913679719 1101 96-97 0.5742721485911702 1101 98-99 0.5681062408992865 1101 100-101 0.6542962600121278 1101 102-103 0.7049534702517128 1101 104-105 0.5552507928681543 1101 106-107 0.6465292192365943 1101 108-109 0.533099085296211 1101 110-111 0.6227055057462181 1101 112-113 0.5895878319131036 1101 114-115 0.628355277111222 1101 116-117 0.5827894557636668 1101 118-119 0.6852898504905411 1101 120-121 0.6762139733224046 1101 122-123 0.6875838326194241 1101 124-125 0.4171811657871274 1102 1 0.39352199516860153 1102 2 0.23370741360831815 1102 3 0.39008121242630267 1102 4 0.42834983407843197 1102 5 0.3286787052714075 1102 6 0.3349911619639485 1102 7 0.32922624342448614 1102 8 0.30408136931506036 1102 9 0.4941421616191022 1102 10-11 0.6691651545316191 1102 12-13 0.33695029753926065 1102 14-15 0.5301253373177346 1102 16-17 0.49384152399297676 1102 18-19 0.5751732842899884 1102 20-21 0.5163919953623974 1102 22-23 0.6575884457074181 1102 24-25 0.7294785830913426 1102 26-27 0.4503844639669481 1102 28-29 0.6427473628555234 1102 30-31 0.6332124762959097 1102 32-33 0.7047411843939599 1102 34-35 0.5730876558903901 1102 36-37 0.6185450789415938 1102 38-39 0.7520649966219395 1102 40-41 0.41769030366690174 1102 42-43 0.6435350115966472 1102 44-45 0.6867241910666309 1102 46-47 0.5464373671731906 1102 48-49 0.5000433487193447 1102 50-51 0.4377799488715972 1102 52-53 0.541792448249037 1102 54-55 0.5435526131017241 1102 56-57 0.4059492829119016 1102 58-59 0.5625087062806067 1102 60-61 0.6306778156645727 1102 62-63 0.6462430056236457 1102 64-65 0.41877621354815275 1102 66-67 0.4779997079600271 1102 68-69 0.5119955832383063 1102 70-71 0.45112559089530535 1102 72-73 0.4949339882283823 1102 74-75 0.5181684712007275 1102 76-77 0.5312254934591856 1102 78-79 0.47194872333164994 1102 80-81 0.484979744730488 1102 82-83 0.35948954235126607 1102 84-85 0.32801967179519664 1102 86-87 0.29593350012764574 1102 88-89 0.35432830383175684 1102 90-91 0.5051990860259465 1102 92-93 0.502260061698717 1102 94-95 0.5185073830061562 1102 96-97 0.5541582290468483 1102 98-99 0.4949175771322025 1102 100-101 0.5418699843725854 1102 102-103 0.5378176757337414 1102 104-105 0.5599317525819281 1102 106-107 0.43787586155593416 1102 108-109 0.4246739870065035 1102 110-111 0.4574649097273351 1102 112-113 0.6203481097203465 1102 114-115 0.5503194832098579 1102 116-117 0.5348202520943524 1102 118-119 0.6730340112141455 1102 120-121 0.5452480242275612 1102 122-123 0.6066741901922477 1102 124-125 0.6590827015390595 1103 1 0.2738048664200825 1103 2 0.32864510250370316 1103 3 0.5306906170612429 1103 4 0.4715741837116809 1103 5 0.4504104657180221 1103 6 0.36406172695052064 1103 7 0.27904356489062465 1103 8 0.41779397687871267 1103 9 0.48940908959222895 1103 10-11 0.7597623116091654 1103 12-13 0.5096710813728436 1103 14-15 0.5134880307152443 1103 16-17 0.27942326530159534 1103 18-19 0.3519565974252643 1103 20-21 0.4067555970099903 1103 22-23 0.5467129680936935 1103 24-25 0.6219964549971522 1103 26-27 0.5894115927167292 1103 28-29 0.5761952969809556 1103 30-31 0.5693914705928833 1103 32-33 0.6544253665189856 1103 34-35 0.6117364922336179 1103 36-37 0.562208586483024 1103 38-39 0.69168870319848 1103 40-41 0.6986693359243574 1103 42-43 0.7832945960201769 1103 44-45 0.8284194069725714 1103 46-47 0.647092475830668 1103 48-49 0.6608349106886351 1103 50-51 0.710945468583855 1103 52-53 0.7237284936622963 1103 54-55 0.7118803665715845 1103 56-57 0.6252130564291356 1103 58-59 0.5350293141683373 1103 60-61 0.7095773249924662 1103 62-63 0.6165170291033668 1103 64-65 0.5461088495089754 1103 66-67 0.6061974341420004 1103 68-69 0.5358149054543127 1103 70-71 0.6003117561655245 1103 72-73 0.4644522093252519 1103 74-75 0.44396575185177056 1103 76-77 0.47937623849053423 1103 78-79 0.44781194897103305 1103 80-81 0.5186504317004648 1103 82-83 0.49059330282463165 1103 84-85 0.5307457092693149 1103 86-87 0.5676982803928325 1103 88-89 0.5547664269128347 1103 90-91 0.5885309996269683 1103 92-93 0.6356222692322859 1103 94-95 0.6589060362670196 1103 96-97 0.7280247053053266 1103 98-99 0.6587953847474957 1103 100-101 0.7302054258506487 1103 102-103 0.5347395698863231 1103 104-105 0.5989862537706578 1103 106-107 0.6246128106508912 1103 108-109 0.6711547230058308 1103 110-111 0.5078844627097894 1103 112-113 0.5956898203577019 1103 114-115 0.6008312872428831 1103 116-117 0.5819975781743025 1103 118-119 0.4305839267937053 1103 120-121 0.415593147901383 1103 122-123 0.49663679633616553 1103 124-125 0.655833155127759 1104 1 0.2548984699332557 1104 2 0.2975502375872523 1104 3 0.30706483948257457 1104 4 0.2889076995025377 1104 5 0.44110105470670646 1104 6 0.276646607286672 1104 7 0.11142052519413781 1104 8 0.26800692468643206 1104 9 0.48798650700790347 1104 10-11 0.5390041878535285 1104 12-13 0.5382969723503948 1104 14-15 0.5826550407639175 1104 16-17 0.6029688099869972 1104 18-19 0.44046455101887005 1104 20-21 0.4245510667119845 1104 22-23 0.45671560302498193 1104 24-25 0.40797288688737154 1104 26-27 0.3689528929712296 1104 28-29 0.4453675182815502 1104 30-31 0.34046123740655787 1104 32-33 0.4838882941315106 1104 34-35 0.5283160074011732 1104 36-37 0.4238810645237763 1104 38-39 0.5587645741846998 1104 40-41 0.43741602601489404 1104 42-43 0.5354310818933499 1104 44-45 0.45532039904713884 1104 46-47 0.44949441701565007 1104 48-49 0.3902899003795355 1104 50-51 0.3988560776479666 1104 52-53 0.46166826789914595 1104 54-55 0.40599937321777446 1104 56-57 0.4197246652891309 1104 58-59 0.4431592111843159 1104 60-61 0.412886100460387 1104 62-63 0.42398356815170146 1104 64-65 0.3802996822415494 1104 66-67 0.3969314587243318 1104 68-69 0.47246239114630484 1104 70-71 0.48423972010009564 1104 72-73 0.48968588109690714 1104 74-75 0.5280309539145627 1104 76-77 0.4097280476868512 1104 78-79 0.21537316716551302 1104 80-81 0.36891358081030745 1104 82-83 0.31384592163798786 1104 84-85 0.35344235503236376 1104 86-87 0.37167984249936126 1104 88-89 0.4500001955194861 1104 90-91 0.5071956526353141 1104 92-93 0.4872170269931644 1104 94-95 0.4109684943317262 1104 96-97 0.3419883503887462 1104 98-99 0.3663370601101974 1104 100-101 0.380489649314228 1104 102-103 0.43791724860359693 1104 104-105 0.37772229455959305 1104 106-107 0.5151848659559093 1104 108-109 0.41505201572860173 1104 110-111 0.5138414715378303 1104 112-113 0.5500688136865328 1104 114-115 0.4000066480360722 1104 116-117 0.4592158322851425 1104 118-119 0.5335001000478279 1104 120-121 0.5418013874895209 1104 122-123 0.5358663745356544 1104 124-125 0.6437715278054057 1105 1 -0.057051704294313055 1105 2 0.15301363473877316 1105 3 0.1562970033679214 1105 4 0.16575350283168433 1105 5 0.15236064324653853 1105 6 0.15263482546717455 1105 7 0.07572796683467686 1105 8 0.024248698946529146 1105 9 0.049746190416684044 1105 10-11 0.41930404901358287 1105 12-13 0.2841152094745709 1105 14-15 0.2992321842750343 1105 16-17 0.2371266749237364 1105 18-19 0.17789516869100197 1105 20-21 0.10422569634663148 1105 22-23 -0.08388567032708494 1105 24-25 0.23544806284667175 1105 26-27 0.2619900394338188 1105 28-29 0.2578353306036618 1105 30-31 0.28075290670847153 1105 32-33 0.38268496438211486 1105 34-35 0.4049852490820669 1105 36-37 0.42541892024467387 1105 38-39 0.5167602192483542 1105 40-41 0.37575572912027866 1105 42-43 0.6464860998582154 1105 44-45 0.7091482856983262 1105 46-47 0.43742309665073975 1105 48-49 0.5423798783036773 1105 50-51 0.5866830366916851 1105 52-53 0.4246467913769507 1105 54-55 0.4763398021458798 1105 56-57 0.6064105617404287 1105 58-59 0.5279754364845104 1105 60-61 0.3754919245101185 1105 62-63 0.4964092315566795 1105 64-65 0.5326829256752603 1105 66-67 0.4296431745873228 1105 68-69 0.5032582813633617 1105 70-71 0.35345685553982165 1105 72-73 0.3866759002815172 1105 74-75 0.5644579936721748 1105 76-77 0.6078460285142917 1105 78-79 0.5474536280780029 1105 80-81 0.6265286959932546 1105 82-83 0.5016393753795896 1105 84-85 0.4482661246724966 1105 86-87 0.5128538682856458 1105 88-89 0.4976763581158252 1105 90-91 0.4732811340726215 1105 92-93 0.44970313546902574 1105 94-95 0.47184461657111854 1105 96-97 0.45570534578383715 1105 98-99 0.44021813360502193 1105 100-101 0.38672615307736535 1105 102-103 0.44552219925668624 1105 104-105 0.4536946259563983 1105 106-107 0.5875047429575666 1105 108-109 0.49260662560641677 1105 110-111 0.4835300905772719 1105 112-113 0.4096164695200706 1105 114-115 0.5294327360925237 1105 116-117 0.4932207022243347 1105 118-119 0.5430867575588891 1105 120-121 0.6498849745596367 1105 122-123 0.7445675132641689 1105 124-125 0.8419241433271694 1106 1 -0.3061583723525416 1106 2 -0.6814985488676513 1106 3 -0.3058406318743323 1106 4 -0.2910099766651655 1106 5 -0.3865026508746148 1106 6 -0.468156574690866 1106 7 -0.5724451134334956 1106 8 -0.6357029443369555 1106 9 -0.4153952268873695 1106 10-11 -0.44389974497587303 1106 12-13 -0.4975435302688851 1106 14-15 -0.6538713676823491 1106 16-17 -0.5865965686042713 1106 18-19 -0.6196054898151644 1106 20-21 -0.7805149764727162 1106 22-23 -0.8789074880028735 1106 24-25 -4.766564356827999 1106 26-27 -1.8293279917073093 1106 28-29 -1.2330992760072377 1106 30-31 -1.224394002288463 1106 32-33 -5.757589272371973 1106 34-35 -1.661260594874335 1106 36-37 -0.8240874486281129 1106 38-39 -0.38680913263249295 1106 40-41 -0.9482993766631509 1106 42-43 -0.5640175703817789 1106 44-45 -0.7435873725344919 1106 46-47 -0.8585340482653407 1106 48-49 -0.558050759875762 1106 50-51 -0.653987297730044 1106 52-53 -0.47821137385094303 1106 54-55 -0.587334388885516 1106 56-57 -0.5813507906572788 1106 58-59 -0.3434798124308216 1106 60-61 -0.40863310717272583 1106 62-63 -0.460650451465785 1106 64-65 -0.24428737445445137 1106 66-67 -0.3452282303417853 1106 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2216 4 -1.1058504736953587 2216 5 -0.7446381475683559 2216 6 -1.061724100651027 2216 7 -0.9622556990408917 2216 8 -0.8289540410891227 2216 9 -1.7428354491756046 2216 10-11 -0.8732417429112047 2216 12-13 -1.2550366494107195 2216 14-15 -1.6213301194610281 2216 16-17 -1.4970984732520698 2216 18-19 -0.9227936617964048 2216 20-21 -0.8810582237125857 2216 22-23 -1.0539113859249127 2216 24-25 -0.860011662666345 2216 26-27 -0.8669380156856832 2216 28-29 -0.9672443345088979 2216 30-31 -0.8766332969533153 2216 32-33 -0.7933311659250535 2216 34-35 -1.0377104987802994 2216 36-37 -1.1664359507592508 2216 38-39 -1.0443496833309212 2216 40-41 -1.2931607665554665 2216 42-43 -0.8419765855779318 2216 44-45 -0.9935145019077538 2216 46-47 -1.0433371580685709 2216 48-49 -1.2135296790927619 2216 50-51 -1.267475913335847 2216 52-53 -1.545336217318603 2216 54-55 -1.4530540930045106 2216 56-57 -1.461433557902918 2216 58-59 -1.472319265638525 2216 60-61 -1.8786392946110766 2216 62-63 -1.9739451557078773 2216 64-65 -1.6498773229034072 2216 66-67 -1.5499128761396612 2216 68-69 -1.5564253086686684 2216 70-71 -1.6432871310343735 2216 72-73 -1.7648450043796942 2216 74-75 -1.6207584119189455 2216 76-77 -1.3448497487447995 2216 78-79 -1.1303736668423099 2216 80-81 -1.283671345423457 2216 82-83 -1.355661675781807 2216 84-85 -1.5900201641091307 2216 86-87 -1.477636782577342 2216 88-89 -1.3299863478327012 2216 90-91 -1.2478534911485895 2216 92-93 -1.1991177620716407 2216 94-95 -1.1872606419795702 2216 96-97 -1.2359021518170614 2216 98-99 -1.1327001049696186 2216 100-101 -1.182225324695775 2216 102-103 -1.2451482778782186 2216 104-105 -1.1596827273327364 2216 106-107 -1.330156594410301 2216 108-109 -1.4330435155305032 2216 110-111 -1.3484170240692848 2216 112-113 -1.5416165759213811 2216 114-115 -1.5981960656383514 2216 116-117 -1.3423754436696278 2216 118-119 -1.3940719546818379 2216 120-121 -1.4575121875462926 2216 122-123 -1.344509612625 2216 124-125 -1.3751708008941854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104218.0 3 16249.0 4 10549.0 5 8530.0 6 8961.0 7 9791.0 8 10483.0 9 11444.0 10 12912.0 11 14116.0 12 14653.0 13 14761.0 14 14650.0 15 13709.0 16 12628.0 17 11317.0 18 10309.0 19 9493.0 20 9188.0 21 9687.0 22 11386.0 23 15374.0 24 22200.0 25 30253.0 26 33411.0 27 33900.0 28 38457.0 29 45241.0 30 52561.0 31 62756.0 32 76247.0 33 96700.0 34 127606.0 35 173214.0 36 252985.0 37 277355.0 38 72492.0 39 109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.449029507653364 18.015126708326548 9.430048260314248 22.105795523705844 2 19.0586265422398 20.940344821674508 37.319202921326486 22.681825714759213 3 16.96064671419722 29.561195776653644 29.578968302043783 23.899189207105355 4 10.843626866908398 16.66280927032348 36.60417684590701 35.88938701686111 5 11.055748489750993 43.19960774385857 34.093824880263014 11.650818886127425 6 30.44281792393096 37.59980661575282 18.836105707578334 13.121269752737897 7 28.4965989076959 34.93241532700261 21.18015017038321 15.39083559491828 8 27.52993496114298 34.361615130663466 19.678411682649692 18.43003822554385 9 28.080387731881647 14.32890119203209 18.343601721043118 39.24710935504315 10-11 24.0058404338608 27.91050192299999 28.338076542828894 19.745581100310307 12-13 24.60266231890394 27.03064892701753 27.77630623610786 20.590382517970667 14-15 22.717387484152248 28.050348214864844 24.739871080636178 24.49239322034673 16-17 22.532714083085597 30.090098739440574 25.66151081973509 21.71567635773874 18-19 21.929071367239906 28.848949128714768 27.21047285278944 22.01150665125589 20-21 23.447317356539887 28.015079002463356 26.84323603644997 21.694367604546787 22-23 23.24782966300721 28.09172211907712 26.747823371346403 21.912624846569273 24-25 22.74973033595497 28.07243426269207 26.894113108887126 22.28372229246584 26-27 22.391304720524793 28.213797718572074 26.812264232351108 22.582633328552024 28-29 22.55777990745614 27.828219533646152 27.053791903755304 22.560208655142404 30-31 22.594553047284737 27.91946864851968 27.00371883174679 22.482259472448785 32-33 22.493848827012528 28.088008848659857 26.951266937334466 22.46687538699314 34-35 22.840344149411433 27.905950095420856 26.863935488129325 22.389770267038386 36-37 22.86840809688188 27.712816310342948 26.69329129624915 22.725484296526023 38-39 22.622961499579684 27.871073224003744 26.967066828125635 22.538898448290936 40-41 22.540317868328348 27.79437737141871 27.255567610381426 22.40973714987152 42-43 22.34100216901269 27.714987560536485 27.129688443371226 22.8143218270796 44-45 22.42424952432276 27.9174751774914 27.075242867555854 22.583032430629984 46-47 22.73832028856968 27.938214909433373 26.632433686578217 22.691031115418728 48-49 22.360859666447226 27.962418166191206 26.907122055811573 22.769600111549995 50-51 22.52873917923327 27.758538319286625 26.98931238556332 22.723410115916785 52-53 22.69251572896716 27.530142841509104 27.004762907553765 22.77257852196998 54-55 22.429509985138942 27.898699078877325 27.047409944307464 22.624380991676265 56-57 22.509226357494637 28.150305335571968 26.80615448619847 22.53431382073493 58-59 22.664725581182708 27.47599835417286 26.939332982833108 22.919943081811326 60-61 22.65070899187887 27.849697036195074 27.058699487221688 22.440894484704373 62-63 22.30773296987932 27.930840873146835 27.046921855958487 22.714504301015356 64-65 22.246531832620477 27.986641365876906 27.02606093733883 22.74076586416379 66-67 22.44681443807102 28.153352715531714 26.617996871227433 22.78183597516983 68-69 22.360005486059123 27.855236614872936 26.945207984501884 22.83954991456606 70-71 22.312821878478804 27.7322668245438 27.123912213609586 22.83099908336781 72-73 22.461719747607063 28.022639130848475 26.59743566811752 22.918205453426943 74-75 22.28419124989964 28.174023659221714 26.84245310486925 22.699331986009394 76-77 22.26912054220706 27.974009720638787 27.127582669718297 22.62928706743586 78-79 22.369231630824373 27.944845612976373 26.89677853668349 22.789144219515762 80-81 22.105897184159424 28.17843562380635 26.708825603496457 23.006841588537768 82-83 22.45721159615747 27.992388092965843 26.707964500625753 22.84243581025093 84-85 22.02372669526092 27.88228144069998 26.951791988675833 23.14219987536327 86-87 21.950970018946922 28.092989924786636 27.092983352939342 22.863056703327096 88-89 22.212796208666223 27.749158444938253 27.213237666121582 22.82480768027394 90-91 22.19689677016195 27.906381356733707 27.084499480394868 22.81222239270947 92-93 22.065024770170876 27.88522916051151 27.005715226183174 23.04403084313444 94-95 21.88536227921068 27.70476828068563 27.25433906713378 23.155530372969903 96-97 22.091658049431263 27.792434300663842 27.126418915076968 22.989488734827926 98-99 21.930161676384927 27.997847857958625 26.864458768564443 23.20753169709201 100-101 21.732348620723254 27.926528865190818 27.175565015862475 23.165557498223453 102-103 21.879071707774248 27.579976912702588 27.146004297437536 23.39494708208563 104-105 21.505082622619557 27.928794137211383 27.271579144674735 23.29454409549433 106-107 21.618147260046953 27.835987985489325 27.25737820335519 23.28848655110853 108-109 21.774388722893523 28.261685148394335 26.648061597313877 23.31586453139827 110-111 21.558280508207 28.28075108114711 26.88048163371436 23.28048677693153 112-113 21.50322320904813 28.036589801681423 27.272654540737356 23.18753244853309 114-115 21.30064049959883 28.251929842206685 27.00344708563539 23.443982572559097 116-117 21.24084008094274 28.196778177233966 27.1268756296864 23.4355061121369 118-119 21.317953165159174 28.043757473758802 27.005996151148153 23.632293209933867 120-121 21.27575495826432 28.063900326964998 27.044345574257456 23.61599914051323 122-123 21.247340892888523 27.977107034329446 27.160623641873098 23.614928430908932 124-125 21.41373169059822 27.831605078386513 26.690016752515096 24.064646478500176 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 257.0 1 320.0 2 379.0 3 361.5 4 331.5 5 322.0 6 335.5 7 334.5 8 332.5 9 345.5 10 361.5 11 366.0 12 391.5 13 447.0 14 516.5 15 612.5 16 719.0 17 868.5 18 1111.5 19 1277.5 20 1611.0 21 2322.0 22 3160.5 23 4306.0 24 5696.0 25 7411.0 26 9659.0 27 11877.0 28 13872.0 29 16935.0 30 21307.0 31 25460.5 32 30059.5 33 34961.0 34 39919.5 35 43816.5 36 48939.0 37 54890.0 38 56370.5 39 56746.5 40 56575.0 41 57032.5 42 57248.0 43 57688.5 44 60905.5 45 63180.0 46 63369.5 47 61617.0 48 60141.5 49 59235.0 50 58844.5 51 57646.5 52 55786.5 53 55431.5 54 54728.0 55 51404.5 56 46515.0 57 42487.0 58 40127.5 59 36768.5 60 31343.5 61 26102.0 62 21379.0 63 16911.0 64 13224.0 65 10414.0 66 7996.0 67 6322.5 68 4936.5 69 3588.5 70 2669.5 71 2087.0 72 1757.5 73 1463.0 74 1095.0 75 861.5 76 578.0 77 362.5 78 272.5 79 186.5 80 124.0 81 84.5 82 61.5 83 39.5 84 26.5 85 20.5 86 15.0 87 11.5 88 9.5 89 6.5 90 5.0 91 4.0 92 5.0 93 4.0 94 1.5 95 2.0 96 2.0 97 3.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.714628592001234E-4 2 2.857314296000617E-4 3 1.7143885776003704E-4 4 5.714628592001234E-4 5 0.0010286331465602222 6 6.286091451201358E-4 7 9.143405747201975E-4 8 0.0010286331465602222 9 0.0014858034339203208 10-11 0.0014286571480003085 12-13 0.0016858154346403643 14-15 0.002371570865680512 16-17 0.0021144125790404566 18-19 0.002142985722000463 20-21 0.0025430097234405494 22-23 0.08851959689009913 24-25 0.001857254292400401 26-27 0.0014858034339203208 28-29 0.0012572182902402715 30-31 0.001000060003600216 32-33 0.0014572302909603148 34-35 8.857674317601913E-4 36-37 6.57182288080142E-4 38-39 0.0010286331465602222 40-41 0.0011143525754402407 42-43 0.001171498861360253 44-45 0.0012286451472802655 46-47 0.001314364576160284 48-49 4.000240014400864E-4 50-51 0.0010857794324802346 52-53 0.0010572062895202283 54-55 0.0011143525754402407 56-57 9.714868606402098E-4 58-59 8.571942888001852E-4 60-61 9.143405747201975E-4 62-63 0.001000060003600216 64-65 8.857674317601913E-4 66-67 4.285971444000926E-4 68-69 2.857314296000617E-4 70-71 6.286091451201358E-4 72-73 2.5715828664005556E-4 74-75 3.714508584800802E-4 76-77 5.714628592001234E-4 78-79 4.000240014400864E-4 80-81 5.143165732801111E-4 82-83 2.857314296000617E-4 84-85 5.428897162401173E-4 86-87 3.714508584800802E-4 88-89 5.428897162401173E-4 90-91 4.857434303201049E-4 92-93 4.5717028736009873E-4 94-95 4.857434303201049E-4 96-97 6.000360021601296E-4 98-99 6.000360021601296E-4 100-101 6.000360021601296E-4 102-103 8.571942888001852E-4 104-105 6.286091451201358E-4 106-107 6.286091451201358E-4 108-109 7.714748599201666E-4 110-111 0.001000060003600216 112-113 4.857434303201049E-4 114-115 6.286091451201358E-4 116-117 9.143405747201975E-4 118-119 0.0012857914332002778 120-121 7.714748599201666E-4 122-123 7.143285740001542E-4 124-125 4.857434303201049E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1749895.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.09215038233659 #Duplication Level Percentage of deduplicated Percentage of total 1 62.31541407402593 23.11412709971344 2 12.271177390280865 9.103287142572531 3 8.160914752978595 9.08117631824734 4 5.378967650102298 7.980699079172723 5 3.496392168417966 6.484435205329158 6 2.214754675700677 4.929000809464357 7 1.4152040065128606 3.67450718818822 8 0.9636361024488764 2.8594668180705947 9 0.6790681692278661 2.26692887875722 >10 2.807234300174858 18.27048088799893 >50 0.18987470551467173 4.838238991310295 >100 0.10455733699929956 6.69821546277012 >500 0.002648852747819611 0.6299086311764435 >1k 1.5581486751880066E-4 0.06952748722866883 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.714628592001234E-5 0.0 14-15 0.0 0.0 0.0 1.1429257184002468E-4 0.0 16-17 0.0 0.0 0.0 1.1429257184002468E-4 0.0 18-19 0.0 0.0 0.0 1.7143885776003704E-4 0.0 20-21 0.0 0.0 0.0 2.2858514368004937E-4 0.0 22-23 0.0 0.0 0.0 3.7145085848008027E-4 0.0 24-25 0.0 0.0 0.0 4.000240014400864E-4 0.0 26-27 0.0 0.0 0.0 4.5717028736009873E-4 0.0 28-29 0.0 0.0 0.0 6.857554310401482E-4 0.0 30-31 0.0 0.0 0.0 0.0025715828664005552 0.0 32-33 0.0 0.0 0.0 0.007514736598481623 0.0 34-35 0.0 0.0 0.0 0.01491518062512322 0.0 36-37 0.0 0.0 0.0 0.027830241243046012 0.0 38-39 0.0 0.0 0.0 0.050288731609610865 0.0 40-41 0.0 0.0 0.0 0.07491878084113618 0.0 42-43 0.0 0.0 0.0 0.10917797925018358 0.0 44-45 0.0 0.0 0.0 0.14789458796099195 0.0 46-47 0.0 0.0 0.0 0.1963260652782024 0.0 48-49 0.0 0.0 0.0 0.24650050431597326 0.0 50-51 0.0 0.0 0.0 0.29844647821726444 0.0 52-53 0.0 0.0 0.0 0.35413553384631646 0.0 54-55 0.0 0.0 0.0 0.42391114895465154 0.0 56-57 0.0 0.0 0.0 0.5134308058483509 0.0 58-59 0.0 0.0 0.0 0.6123510267758923 0.0 60-61 0.0 0.0 0.0 0.7135285259972741 0.0 62-63 0.0 0.0 0.0 0.8136202457861758 0.0 64-65 0.0 0.0 0.0 0.9319987770694813 0.0 66-67 0.0 0.0 0.0 1.0779503913091928 0.0 68-69 0.0 0.0 0.0 1.254218110229471 0.0 70-71 0.0 0.0 0.0 1.4283142702847886 0.0 72-73 0.0 0.0 0.0 1.6034676366296263 0.0 74-75 0.0 0.0 0.0 1.7980507401872683 0.0 76-77 0.0 0.0 0.0 2.0302646730232388 0.0 78-79 0.0 0.0 0.0 2.300738044282657 0.0 80-81 0.0 0.0 0.0 2.5890696298920792 0.0 82-83 0.0 0.0 0.0 2.888401875541104 0.0 84-85 0.0 0.0 0.0 3.1914486297749294 0.0 86-87 0.0 0.0 0.0 3.536469331017004 0.0 88-89 0.0 0.0 0.0 3.9290071690015687 0.0 90-91 0.0 0.0 0.0 4.355318461964861 0.0 92-93 0.0 0.0 0.0 4.782858400075433 0.0 94-95 0.0 0.0 0.0 5.224713482808968 0.0 96-97 0.0 0.0 0.0 5.712971349709553 0.0 98-99 0.0 0.0 0.0 6.237231376739747 0.0 100-101 0.0 0.0 0.0 6.815437497678432 0.0 102-103 0.0 0.0 0.0 7.396129482054638 0.0 104-105 0.0 0.0 0.0 7.9987370670811675 0.0 106-107 0.0 0.0 0.0 8.623088813900264 0.0 108-109 0.0 0.0 0.0 9.272499207095283 0.0 110-111 0.0 0.0 0.0 9.984341917657916 0.0 112-113 0.0 0.0 0.0 10.704785144251513 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 65.634445 1 GTACATG 15070 0.0 52.150833 1 TACATGG 15800 0.0 48.687016 2 GTATCAA 4045 0.0 47.80095 2 ACATGGG 15550 0.0 47.327225 3 CATGGGA 9990 0.0 43.771713 4 CATGGGG 5120 0.0 40.901985 4 ATGGGAT 4185 0.0 39.236053 5 ATCAACG 5035 0.0 38.992954 4 CAACGCA 5150 0.0 38.468807 6 TCAACGC 5115 0.0 38.383095 5 AACGCAG 5315 0.0 37.0507 7 ATGGGAG 3975 0.0 35.471775 5 ATGGGAC 1910 0.0 34.263443 5 TATCAAC 6045 0.0 32.576424 3 TACCTGG 1535 0.0 32.169285 2 GACCGTA 75 0.003913036 31.730026 7 ATGGGGA 2530 0.0 30.80509 5 ACGCAGA 6400 0.0 30.211695 8 GAGTACA 9250 0.0 29.586105 1 >>END_MODULE