FastQCFastQC Report
Thu 26 May 2016
SRR936222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749895
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67990.3885375979701639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.2712734192622986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45740.26138711179813645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51950.084.871461
GTATCAA80350.054.7506072
GTACATG133650.048.0133741
TACATGG141750.045.2716262
ACATGGG138550.045.114193
CATGGGA89950.043.6128654
ATCAACG115150.039.548314
ATGGGAT37500.039.527425
TCAACGC116200.039.29345
CAACGCA117000.039.1549766
AACGCAG120350.038.1145177
TATCAAC122350.037.3669473
CATGGGG43500.036.949194
ATGGGAG34400.034.0908785
ACGCAGA137800.032.9857448
CGCAGAG140650.032.190459
GTCGGGA5650.030.5548342
AGTACAT85550.029.2253462
TACCTGG13650.029.2194822
GAGTACA89650.028.9768831
Produced by FastQC (version 0.11.5)