##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936222_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1749895 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51998777069481 31.0 31.0 34.0 30.0 34.0 2 31.306405812920204 31.0 31.0 34.0 28.0 34.0 3 31.665854236968503 31.0 31.0 34.0 30.0 34.0 4 35.53580357678604 37.0 35.0 37.0 33.0 37.0 5 35.19191951517091 37.0 35.0 37.0 33.0 37.0 6 35.39468425248372 37.0 35.0 37.0 33.0 37.0 7 35.2056403384203 37.0 35.0 37.0 32.0 37.0 8 35.289553373202395 37.0 35.0 37.0 32.0 37.0 9 36.92884315916098 39.0 37.0 39.0 33.0 39.0 10-11 36.8249560687927 39.0 37.0 39.0 32.0 39.0 12-13 36.79256555393323 39.0 37.0 39.0 32.0 39.0 14-15 37.916705288031565 40.0 37.0 41.0 32.5 41.0 16-17 37.83065383923035 40.0 37.0 41.0 32.0 41.0 18-19 37.950912769051854 40.0 37.0 41.0 32.0 41.0 20-21 38.00156695115993 40.0 37.0 41.0 32.5 41.0 22-23 37.91332822826512 40.0 37.0 41.0 32.0 41.0 24-25 37.886284605647774 40.0 37.0 41.0 32.0 41.0 26-27 37.715124907494456 40.0 37.0 41.0 32.0 41.0 28-29 37.56552736021304 40.0 36.5 41.0 32.0 41.0 30-31 37.37397129542058 39.5 36.0 41.0 31.5 41.0 32-33 37.1566499704268 39.0 36.0 41.0 31.0 41.0 34-35 36.948681492318116 39.0 35.0 41.0 31.0 41.0 36-37 36.73393860774503 39.0 35.0 41.0 30.0 41.0 38-39 36.70694784544216 39.0 35.0 41.0 30.5 41.0 40-41 36.718891133468006 39.0 35.0 41.0 30.0 41.0 42-43 36.59139748384903 39.0 35.0 41.0 30.0 41.0 44-45 36.400860908797384 39.0 35.0 41.0 30.0 41.0 46-47 36.0762814340289 39.0 35.0 41.0 29.5 41.0 48-49 35.76801636669629 39.0 35.0 41.0 28.0 41.0 50-51 35.562139728383706 39.0 35.0 41.0 27.0 41.0 52-53 35.409503141617066 39.0 35.0 41.0 27.0 41.0 54-55 35.227115341206186 39.0 35.0 41.0 26.0 41.0 56-57 35.02638158289497 38.5 34.0 40.5 26.0 41.0 58-59 34.80633609445138 38.0 34.0 40.0 26.0 41.0 60-61 34.57339068915564 38.0 34.0 40.0 26.0 41.0 62-63 34.3380234242626 37.5 34.0 40.0 25.5 41.0 64-65 34.07777695233142 37.0 34.0 40.0 25.0 41.0 66-67 33.790786018589685 36.5 33.0 39.5 25.0 41.0 68-69 33.47517079596204 36.0 33.0 39.0 24.0 41.0 70-71 33.1468965280774 35.5 33.0 39.0 23.5 41.0 72-73 32.88391760648496 35.0 33.0 38.5 23.5 40.0 74-75 32.698525340091834 35.0 33.0 37.5 24.0 40.0 76-77 32.400402881315735 35.0 33.0 37.0 23.5 39.0 78-79 32.08334871520863 35.0 32.0 36.5 23.0 39.0 80-81 31.816179827932533 35.0 32.0 36.0 23.0 38.5 82-83 31.570541375339666 35.0 32.0 36.0 22.5 37.0 84-85 31.324456895985186 35.0 32.0 35.0 21.5 37.0 86-87 31.142503978810158 35.0 32.0 35.0 21.0 36.5 88-89 30.996902957034564 35.0 32.0 35.0 20.0 36.0 90-91 30.871330851279648 35.0 32.0 35.0 20.0 36.0 92-93 30.75286174313316 35.0 32.0 35.0 20.0 36.0 94-95 30.640211555550476 34.5 32.0 35.0 20.0 35.0 96-97 30.58826815323205 34.0 32.0 35.0 19.0 35.0 98-99 30.512166158540943 34.0 32.0 35.0 19.0 35.0 100-101 30.43588158146632 34.0 31.0 35.0 18.5 35.0 102-103 29.40878252695162 33.5 29.5 34.5 17.0 35.0 104-105 30.169553601787534 34.0 31.0 35.0 18.0 35.0 106-107 30.330480685984014 34.0 31.0 35.0 18.0 35.0 108-109 30.307070995688314 34.0 31.5 35.0 17.5 35.0 110-111 30.27552195988902 34.0 31.5 35.0 17.0 35.0 112-113 30.196224344889266 34.0 31.0 35.0 15.0 35.0 114-115 30.179445052417428 34.0 32.0 35.0 10.5 35.0 116-117 30.084214195708885 34.0 31.5 35.0 7.0 35.0 118-119 29.953800370879396 34.0 31.0 35.0 2.0 35.0 120-121 29.762757194003072 34.0 31.0 35.0 2.0 35.0 122-123 29.53562385171682 34.0 31.0 35.0 2.0 35.0 124-125 27.447834870092205 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11280430866458246 1101 2 0.5820663807887811 1101 3 0.30166291203886786 1101 4 0.23279508004241478 1101 5 0.5433941910952313 1101 6 0.30907991556474457 1101 7 0.47574978247180866 1101 8 0.4522811051158371 1101 9 0.26647651611889955 1101 10-11 0.3723017136747231 1101 12-13 0.4413947124544251 1101 14-15 0.5028118426203179 1101 16-17 0.42645713499088345 1101 18-19 0.42127236077400454 1101 20-21 0.4747187436529714 1101 22-23 0.44389234615573514 1101 24-25 0.33762136134761533 1101 26-27 0.3159025948820826 1101 28-29 0.38523709703949294 1101 30-31 0.32491284451849367 1101 32-33 0.2526920470589076 1101 34-35 0.36926929115567475 1101 36-37 0.40172752309152315 1101 38-39 0.3736587998649199 1101 40-41 0.33119456197020014 1101 42-43 0.29298340497508946 1101 44-45 0.21094538188258838 1101 46-47 0.19234459001551585 1101 48-49 0.11084855826934614 1101 50-51 0.13989949304266958 1101 52-53 0.06155078823716309 1101 54-55 0.1300530766735548 1101 56-57 -0.1317106347391288 1101 58-59 0.02455535235244355 1101 60-61 0.14382830648014533 1101 62-63 0.11573503641444205 1101 64-65 0.07586383894629734 1101 66-67 0.08535595675911622 1101 68-69 -0.06450894796678597 1101 70-71 -0.02820429575463379 1101 72-73 -0.014549604305585717 1101 74-75 0.035438525416367384 1101 76-77 0.11497747573464778 1101 78-79 0.13079765894989492 1101 80-81 0.07930088640555155 1101 82-83 0.1250419119570232 1101 84-85 0.08468224382856349 1101 86-87 0.13161084182497262 1101 88-89 -0.0017670673090997013 1101 90-91 0.1373270830233011 1101 92-93 0.11529985810962273 1101 94-95 0.09814578222475134 1101 96-97 0.08498247916973156 1101 98-99 0.09369440468528012 1101 100-101 0.08869075418464334 1101 102-103 0.07321029575869886 1101 104-105 0.04132158985740375 1101 106-107 0.11893396677205459 1101 108-109 -0.0029856084961643603 1101 110-111 0.07453180280384686 1101 112-113 0.17511852246234838 1101 114-115 0.13298818087740472 1101 116-117 0.011691507817321423 1101 118-119 0.01577020383369998 1101 120-121 0.12779549607677154 1101 122-123 0.10189659696917275 1101 124-125 0.13134582881091816 1102 1 0.20558478589898144 1102 2 0.5338611031256235 1102 3 0.37833209422745995 1102 4 0.2429548188308246 1102 5 0.4398826659402033 1102 6 0.3438275913495801 1102 7 0.4565657503355496 1102 8 0.32631146830863145 1102 9 0.3475542165518206 1102 10-11 0.413812104013509 1102 12-13 0.44386788955359435 1102 14-15 0.45443724856124845 1102 16-17 0.5257760816528858 1102 18-19 0.5530056761878583 1102 20-21 0.4476881455187325 1102 22-23 0.4910812850069064 1102 24-25 0.3793335345484081 1102 26-27 0.4198784030863081 1102 28-29 0.46873065489982935 1102 30-31 0.30767101175375444 1102 32-33 0.3111721432905199 1102 34-35 0.27546624607799686 1102 36-37 0.3021560175245952 1102 38-39 0.2753114562447436 1102 40-41 0.2020169740499398 1102 42-43 0.2729975266294531 1102 44-45 0.31464799358327866 1102 46-47 0.2793023072253007 1102 48-49 0.07252441181336877 1102 50-51 0.2109299547674084 1102 52-53 0.22834077713941525 1102 54-55 0.20223351824517266 1102 56-57 0.13507342714547832 1102 58-59 0.17040667280011945 1102 60-61 0.16776137723779527 1102 62-63 0.19058842452298563 1102 64-65 0.1472730761089167 1102 66-67 0.10616576606141592 1102 68-69 0.024463011451132388 1102 70-71 0.06138351980715129 1102 72-73 0.1459937358469645 1102 74-75 0.017118503963331477 1102 76-77 0.13985194817029623 1102 78-79 0.08715656239375136 1102 80-81 0.03804374708628444 1102 82-83 0.12921020351998536 1102 84-85 0.21038359185385502 1102 86-87 0.10799727632871736 1102 88-89 0.17884451282499825 1102 90-91 0.1438557370076623 1102 92-93 0.17344100695188303 1102 94-95 0.07381279507455218 1102 96-97 0.09799605808041889 1102 98-99 0.15716357746111598 1102 100-101 0.232782278914339 1102 102-103 0.19692389068869431 1102 104-105 0.13092425442306066 1102 106-107 0.0778458921706573 1102 108-109 0.08851634433660038 1102 110-111 0.052294853222129234 1102 112-113 0.07213419986446823 1102 114-115 0.10415783313764138 1102 116-117 0.2116222711907696 1102 118-119 0.16268970876791045 1102 120-121 0.1973423158039509 1102 122-123 0.2793126211332826 1102 124-125 0.2652874931149576 1103 1 0.15921973599823147 1103 2 0.6673748749707293 1103 3 0.39757192790298035 1103 4 0.2685334733259026 1103 5 0.5164515427073155 1103 6 0.2949705010991579 1103 7 0.33876608049587276 1103 8 0.35186955246001617 1103 9 0.3955205983003012 1103 10-11 0.4956243992486762 1103 12-13 0.3890210433343171 1103 14-15 0.5848684976139751 1103 16-17 0.6917233541830612 1103 18-19 0.5883307614443609 1103 20-21 0.4862459927956664 1103 22-23 0.5635999494388457 1103 24-25 0.461076736144463 1103 26-27 0.5373024433062525 1103 28-29 0.5417950013326873 1103 30-31 0.4393401411827398 1103 32-33 0.5201764894381711 1103 34-35 0.41462720826024224 1103 36-37 0.371586543334125 1103 38-39 0.399003267569519 1103 40-41 0.42468879920352975 1103 42-43 0.41276777482011795 1103 44-45 0.37047773625585023 1103 46-47 0.40769576716846956 1103 48-49 0.3063028974801867 1103 50-51 0.2690040733755126 1103 52-53 0.26517822435219074 1103 54-55 0.20910363444490088 1103 56-57 0.238195525764759 1103 58-59 0.20415358362035363 1103 60-61 0.09981450324224994 1103 62-63 0.0830666701225411 1103 64-65 0.1210952945788435 1103 66-67 0.06841615661399914 1103 68-69 0.15409845321946136 1103 70-71 0.10889760639113177 1103 72-73 0.16982353253246174 1103 74-75 0.1712582099134181 1103 76-77 0.30856978097984467 1103 78-79 0.2353261362304906 1103 80-81 0.25434072176923905 1103 82-83 0.05531080209174277 1103 84-85 0.11961846466918402 1103 86-87 0.15689506557204425 1103 88-89 0.2408319815735318 1103 90-91 0.17970915198127813 1103 92-93 0.13672344824532345 1103 94-95 0.10508444993598687 1103 96-97 0.15637620787543227 1103 98-99 0.2006127495931196 1103 100-101 0.22590818746414598 1103 102-103 0.21941011581482783 1103 104-105 0.16763562940667143 1103 106-107 0.11596904896374838 1103 108-109 0.12838047153755028 1103 110-111 0.18751407061772696 1103 112-113 0.1855208999888589 1103 114-115 0.22871271550871697 1103 116-117 0.18304400627023298 1103 118-119 0.08162021747755688 1103 120-121 0.06096251456899893 1103 122-123 0.17516900934185742 1103 124-125 0.26106079227880485 1104 1 0.11508247085814105 1104 2 0.36582100451404287 1104 3 0.19555513518517742 1104 4 0.14976165200482683 1104 5 0.4287767523487176 1104 6 0.1798131735310733 1104 7 0.2636316773396601 1104 8 0.2809851864451218 1104 9 0.45822202465434714 1104 10-11 0.35436755486175286 1104 12-13 0.24941619128928494 1104 14-15 0.3204146635723504 1104 16-17 0.30360179771844287 1104 18-19 0.2739005755288062 1104 20-21 0.2681928145818162 1104 22-23 0.30629451324941925 1104 24-25 0.25181611213463384 1104 26-27 0.14910150762533192 1104 28-29 0.22841299057431286 1104 30-31 0.27042390829864615 1104 32-33 0.2542341358227489 1104 34-35 0.2813781723418458 1104 36-37 0.29588909562438914 1104 38-39 0.3632835758411872 1104 40-41 0.2390568705637932 1104 42-43 0.26002088443402016 1104 44-45 0.2911537541254745 1104 46-47 0.25245301838980083 1104 48-49 0.38622209631837023 1104 50-51 0.3336608022229868 1104 52-53 0.41857359069555145 1104 54-55 0.37882378894259006 1104 56-57 0.3887100508871981 1104 58-59 0.4807421171842563 1104 60-61 0.38686994459196455 1104 62-63 0.3846711241247718 1104 64-65 0.39797095665628746 1104 66-67 0.2734966303206221 1104 68-69 0.36978955823676074 1104 70-71 0.378253457429345 1104 72-73 0.37840650267084186 1104 74-75 0.2954990426332529 1104 76-77 0.246390945084336 1104 78-79 0.35440980447276615 1104 80-81 0.3915334904373111 1104 82-83 0.3377374030736995 1104 84-85 0.402825288172874 1104 86-87 0.4007153999534907 1104 88-89 0.38100147085300407 1104 90-91 0.4300414489252944 1104 92-93 0.5391512290852951 1104 94-95 0.48204774768755243 1104 96-97 0.47141702764995586 1104 98-99 0.41091638847400347 1104 100-101 0.3882277908687648 1104 102-103 0.4420557575645745 1104 104-105 0.46497609438285536 1104 106-107 0.5176610749328532 1104 108-109 0.4785095169151923 1104 110-111 0.4878411945341625 1104 112-113 0.5005092887079954 1104 114-115 0.4792752504043598 1104 116-117 0.49575237671303185 1104 118-119 0.484704055435909 1104 120-121 0.4446720900978782 1104 122-123 0.4514974262603566 1104 124-125 0.4199709755237535 1105 1 0.22781237746493588 1105 2 0.6051316837455722 1105 3 0.19743293796016914 1105 4 0.2307902723091857 1105 5 0.4613935992663798 1105 6 0.2774438592679047 1105 7 0.33748489249339286 1105 8 0.2883766819712221 1105 9 0.3349172423559921 1105 10-11 0.2102966380663318 1105 12-13 0.23837751186500356 1105 14-15 0.2622648941189425 1105 16-17 0.3792360643814945 1105 18-19 0.29141255461547644 1105 20-21 0.2978299483952114 1105 22-23 0.3367353978496297 1105 24-25 0.3058462352835605 1105 26-27 0.20615689603084775 1105 28-29 0.34061885919739154 1105 30-31 0.20778555775337537 1105 32-33 0.1698493383453581 1105 34-35 0.22945022455289177 1105 36-37 0.25749277232306866 1105 38-39 0.11833421778771935 1105 40-41 0.2077237661206155 1105 42-43 0.14752099737324187 1105 44-45 0.15812565785908816 1105 46-47 0.1729637596626148 1105 48-49 -0.012563280010162714 1105 50-51 -0.008868418918027032 1105 52-53 -0.020513017122247845 1105 54-55 -0.0787295108402617 1105 56-57 -0.035048822370903565 1105 58-59 0.0934102836837738 1105 60-61 -0.11654910208054048 1105 62-63 -0.06615476274928511 1105 64-65 0.014428986936053434 1105 66-67 0.013678447407272643 1105 68-69 0.05556064700095931 1105 70-71 0.10788110434820908 1105 72-73 0.022962681650895433 1105 74-75 0.10226417724175008 1105 76-77 0.07503278319273932 1105 78-79 0.14563374939456963 1105 80-81 0.11731819214223549 1105 82-83 -0.030325117067125262 1105 84-85 0.09745156476253314 1105 86-87 0.12487565920170596 1105 88-89 0.03600113956462536 1105 90-91 -0.060080886838491665 1105 92-93 -0.00922816839149121 1105 94-95 0.06932491860525403 1105 96-97 0.01766601496160547 1105 98-99 0.07179567301157164 1105 100-101 0.04719501440107976 1105 102-103 -0.006439816723833758 1105 104-105 0.042060124174714275 1105 106-107 0.05734227010086457 1105 108-109 0.13845366470595266 1105 110-111 0.1587566346808913 1105 112-113 0.08625613318616843 1105 114-115 0.19487923429771925 1105 116-117 0.17758715717424778 1105 118-119 0.1577310825443341 1105 120-121 0.10918048825832471 1105 122-123 0.1404311896662449 1105 124-125 0.14536189581275494 1106 1 0.12370528696879646 1106 2 0.5098962864302905 1106 3 0.19916890929403053 1106 4 0.09674525533712597 1106 5 0.34179496498330764 1106 6 0.15982770126618107 1106 7 0.2612850445710464 1106 8 0.19147315188121894 1106 9 0.17961688899909944 1106 10-11 0.21090755783792048 1106 12-13 0.16357543645238337 1106 14-15 0.08716897586316463 1106 16-17 0.14296666329906316 1106 18-19 0.12438445503940443 1106 20-21 0.14523357042758533 1106 22-23 0.02822004347391527 1106 24-25 0.04726055741980417 1106 26-27 0.17160222014011595 1106 28-29 0.11730898359293462 1106 30-31 0.07987185436442701 1106 32-33 0.12372992757169499 1106 34-35 0.06195930696804908 1106 36-37 0.06504124086138319 1106 38-39 0.17391114373186412 1106 40-41 0.06883040435969434 1106 42-43 0.04601624867409981 1106 44-45 0.0728776868035439 1106 46-47 0.132354678738416 1106 48-49 0.13141747670038484 1106 50-51 0.14758233227597373 1106 52-53 0.11026680326708771 1106 54-55 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-0.8752271621577634 2216 76-77 -0.7168070791522183 2216 78-79 -0.6405401400463262 2216 80-81 -0.6243686602725091 2216 82-83 -0.7938994610466459 2216 84-85 -0.6541839544456103 2216 86-87 -0.725643650197469 2216 88-89 -0.7680769858130425 2216 90-91 -0.801874792310393 2216 92-93 -0.7866448564524369 2216 94-95 -0.8697190216527702 2216 96-97 -0.7817827801974708 2216 98-99 -0.9396454489501949 2216 100-101 -1.1484785395905703 2216 102-103 -1.1437116410124482 2216 104-105 -0.9879114655280716 2216 106-107 -0.950034101991065 2216 108-109 -0.9767784583317329 2216 110-111 -0.9819042231938013 2216 112-113 -0.8203580799322161 2216 114-115 -0.9725842468096637 2216 116-117 -0.9973263511414316 2216 118-119 -1.0757008441232614 2216 120-121 -1.1122569231840558 2216 122-123 -1.3150313089374102 2216 124-125 -1.5859359922615184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52.0 3 67.0 4 150.0 5 340.0 6 896.0 7 1819.0 8 2971.0 9 5240.0 10 8984.0 11 11006.0 12 12054.0 13 14796.0 14 20386.0 15 15193.0 16 7669.0 17 6586.0 18 6285.0 19 5870.0 20 5828.0 21 5949.0 22 6538.0 23 7768.0 24 9894.0 25 12924.0 26 16995.0 27 21922.0 28 28076.0 29 35978.0 30 45675.0 31 56888.0 32 71640.0 33 92050.0 34 122500.0 35 173029.0 36 288162.0 37 451330.0 38 175712.0 39 673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.53816017535102 18.5052389717971 10.794231898627626 22.16236895422426 2 19.36184377830543 21.35820353908971 36.35176025853127 22.928192424073583 3 17.43449456623919 28.016055838842718 31.816345342258067 22.73310425266002 4 11.681736150875425 16.412937012184738 36.77862773402275 35.126699102917094 5 10.82177242925939 41.553097056701134 35.16691501977866 12.458215494260813 6 30.554153882551677 37.57064003341918 18.036759961894813 13.838446122134323 7 27.015963814971755 34.161535406410096 22.611699559116406 16.210801219501743 8 27.819669180150807 32.76648027453076 20.253100900339735 19.160749644978697 9 27.502793024724344 11.456287377242633 19.612605327748238 41.42831427028479 10-11 24.4393806485532 25.673626131853627 29.566002531580466 20.32099068801271 12-13 25.533503438777753 25.178167832927116 29.420050917340756 19.86827781095437 14-15 22.96827037287105 26.265526293156533 27.242499476397004 23.523703857575416 16-17 21.57478020109778 28.483680449398392 27.92818997711291 22.013349372390913 18-19 20.856337094511385 26.464559302129558 31.074693053011753 21.604410550347307 20-21 22.179245040416713 25.74234454067244 31.337623114529727 20.74078730438112 22-23 21.788707322439347 25.984130476400015 31.238302869600748 20.988859331559894 24-25 21.57732321082122 26.178342129099175 31.122753079470485 21.12158158060912 26-27 21.194471668300096 26.237888559027827 31.14821174984785 21.419428022824228 28-29 21.390712014149422 25.888896190914313 31.37425388380446 21.34613791113181 30-31 21.396883813028783 25.877181202300708 31.3972266907443 21.32870829392621 32-33 21.24944639535515 26.270576234574072 31.052120270073345 21.42785709999743 34-35 21.694398417728664 26.101991011432435 30.880492739684417 21.32311783115448 36-37 21.81008003337343 26.009932024492898 30.447912589041053 21.732075353092615 38-39 21.711302678160692 26.183428148546056 30.511973575557388 21.593295597735864 40-41 21.629618242699774 26.15280521197135 30.63321817400992 21.584358371318956 42-43 21.65288543029289 26.243754439684018 30.18865121042288 21.914708919600216 44-45 21.665730798631202 26.46537645470327 29.89902626860127 21.969866478064255 46-47 22.199957820834566 26.592334936865562 29.12997184054904 22.077735401750836 48-49 22.1046796058798 26.867927439818946 28.667072445877057 22.360320508424206 50-51 22.269162221035387 26.864471897866636 28.622207210566756 22.244158670531217 52-53 22.24620950182024 26.819639152574336 28.573383930459435 22.36076741514599 54-55 21.99806029654488 27.220600405085975 28.49498100302214 22.286358295346997 56-57 22.2488317189519 27.18835885158987 28.30226203899547 22.260547390462758 58-59 22.276281658202002 26.667497226862373 28.414759664263183 22.64146145067244 60-61 22.271963050504958 26.990830060668124 28.717780058610803 22.01942683021611 62-63 22.032150470993344 26.88815226084519 28.924880226508837 22.15481704165263 64-65 21.98216423646531 26.821536641607445 28.863048652602647 22.3332504693246 66-67 22.132715830668335 27.091872189749488 28.470709388951242 22.30470259063094 68-69 22.153499578253903 26.889599672661024 28.575641134074115 22.38125961501096 70-71 22.089835590776534 26.79492022418037 28.62640091299724 22.488843272045862 72-73 22.199388401574772 27.138179983209902 27.991257491394933 22.671174123820393 74-75 22.023087875479426 27.298136814563517 28.108964074383408 22.56981123557365 76-77 21.941749903065087 27.141032941869625 28.42623131627755 22.49098583878774 78-79 22.06568866289386 27.11668898234233 28.20495676587856 22.61266558888525 80-81 21.75860498420755 27.422942167122795 27.8239966352113 22.994456213458356 82-83 22.27268882879629 27.091100479343865 27.800058517897103 22.836152173962738 84-85 21.693769806607786 27.107940244226853 27.941278577875917 23.257011371289444 86-87 21.780330575348085 27.145129532538785 28.03198603796756 23.042553854145563 88-89 22.085421349165394 26.927446485932933 27.948266925429188 23.038865239472486 90-91 21.910962667616836 27.182236994184965 27.92323849630385 22.983561841894346 92-93 21.906506507707785 27.10301496697072 27.695081938171416 23.29539658715008 94-95 21.794976931097317 26.855705849560525 27.952360937248642 23.39695628209352 96-97 21.965141852493065 27.033137156126752 27.869059500926785 23.132661490453398 98-99 21.85444031363661 27.068001745282977 27.56561312493946 23.511944816140954 100-101 21.680349989813585 26.920510812315936 27.74731060052992 23.65182859734056 102-103 21.923734661426302 26.531657216005122 27.750256803690544 23.794351318878036 104-105 21.691431634487877 26.629614697427172 27.905327465898466 23.773626202186488 106-107 21.632266330492385 26.6330206653462 27.904017747441834 23.83069525671958 108-109 21.83470347529644 26.831447536816107 27.273563693957215 24.06028529393024 110-111 21.666732861738687 26.96987247971788 27.361756830545662 24.001637827997772 112-113 21.653464327966667 26.711703215998263 27.651755453823252 23.983077002211818 114-115 21.357873395320816 26.98078814833783 27.364899430863687 24.296439025477667 116-117 21.184445214814694 26.955686975766774 27.550764652808958 24.30910315660957 118-119 21.243143671831284 26.922529564298454 27.314005398760106 24.520321365110156 120-121 21.168094709455087 27.026456296461397 27.35887093893635 24.44657805514717 122-123 21.140175640678706 27.056082657933306 27.228991542009485 24.5747501593785 124-125 21.174701971123937 26.986261482459167 26.81215472612833 25.026881820288565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 17.0 2 78.5 3 173.0 4 191.0 5 211.5 6 325.5 7 736.0 8 1278.5 9 1698.0 10 1846.0 11 1832.0 12 1988.5 13 2214.5 14 2474.5 15 2806.0 16 3092.0 17 3274.0 18 3534.0 19 3831.5 20 4121.0 21 4615.0 22 5303.5 23 6319.5 24 7737.5 25 9266.5 26 11198.0 27 13210.0 28 15092.5 29 17882.5 30 21966.5 31 26168.5 32 30173.0 33 34816.5 34 39912.0 35 43031.0 36 47572.5 37 53729.0 38 55207.5 39 55641.5 40 55263.5 41 54941.0 42 55582.0 43 56761.0 44 59949.0 45 62462.5 46 62439.0 47 60440.0 48 59188.0 49 58442.0 50 58924.5 51 56913.0 52 53634.0 53 53909.0 54 53953.5 55 50781.5 56 45363.0 57 41387.0 58 39200.5 59 35545.0 60 30036.0 61 24788.5 62 20466.0 63 15865.5 64 12004.5 65 9317.5 66 6801.0 67 5243.0 68 4022.0 69 2809.0 70 2168.5 71 1669.0 72 1275.0 73 1108.0 74 947.5 75 744.0 76 453.0 77 258.0 78 144.5 79 77.0 80 26.0 81 8.0 82 6.0 83 2.5 84 0.5 85 0.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06708973967009449 2 5.714628592001234E-4 3 1.1429257184002468E-4 4 5.714628592001234E-5 5 0.05948928364273285 6 1.1429257184002468E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.857314296000617E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.143045725600679E-4 36-37 0.0 38-39 0.0 40-41 2.857314296000617E-5 42-43 0.0029716068678406417 44-45 0.010429197180402253 46-47 0.012343597758722666 48-49 0.01000060003600216 50-51 0.00820049202952177 52-53 0.007143285740001542 54-55 0.008171918886561765 56-57 0.005828921163841259 58-59 0.004114532586240889 60-61 0.0026858754382405804 62-63 0.0016858154346403643 64-65 0.0022858514368004935 66-67 0.0029144605819206295 68-69 0.0015429497198403332 70-71 0.0030858994396806665 72-73 0.0030858994396806665 74-75 8.857674317601913E-4 76-77 5.428897162401173E-4 78-79 2.2858514368004937E-4 80-81 4.5717028736009873E-4 82-83 1.7143885776003704E-4 84-85 3.143045725600679E-4 86-87 3.714508584800802E-4 88-89 0.0016000960057603456 90-91 0.0019144005783204134 92-93 4.857434303201049E-4 94-95 0.001628669148720352 96-97 0.0018001080064803886 98-99 0.002028693150160438 100-101 8.857674317601913E-4 102-103 0.001000060003600216 104-105 6.000360021601296E-4 106-107 5.143165732801111E-4 108-109 8.857674317601913E-4 110-111 9.429137176802037E-4 112-113 0.01171498861360253 114-115 0.02297280693984496 116-117 0.021458430362964635 118-119 0.01337223090528289 120-121 0.007000420025201513 122-123 0.007400444026641598 124-125 0.006086079450481315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1749895.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.513238882539945 #Duplication Level Percentage of deduplicated Percentage of total 1 41.534453580018095 10.596784360427682 2 17.06637373724186 8.708369400339153 3 12.365575489605742 9.464576441591763 4 8.600469208786715 8.777033017028192 5 5.77610122828576 7.36835252234935 6 3.858106119709242 5.905966983979869 7 2.507199608509487 4.477674777667922 8 1.7105999911736325 3.491435696582689 9 1.130198181944335 2.5951514580502346 >10 4.907629381229822 21.834187091048715 >50 0.35063481381821315 6.171904655252567 >100 0.18517026675205184 8.416315455212436 >500 0.006353787936424849 1.121076770507869 >1k 9.076839909178356E-4 0.6775439705253868 >5k 2.269209977294589E-4 0.393627399436222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6799 0.3885375979701639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4747 0.2712734192622986 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4574 0.26138711179813645 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.714628592001234E-5 0.0 16-17 0.0 0.0 0.0 5.714628592001234E-5 0.0 18-19 0.0 0.0 0.0 1.7143885776003704E-4 0.0 20-21 0.0 0.0 0.0 2.2858514368004937E-4 0.0 22-23 0.0 0.0 0.0 3.7145085848008027E-4 0.0 24-25 0.0 0.0 0.0 4.000240014400864E-4 0.0 26-27 0.0 0.0 0.0 4.2859714440009254E-4 0.0 28-29 0.0 0.0 0.0 6.571822880801419E-4 0.0 30-31 0.0 0.0 0.0 0.002657302295280574 0.0 32-33 0.0 0.0 0.0 0.0076576023132816536 0.0 34-35 0.0 0.0 0.0 0.015258058340643296 0.0 36-37 0.0 0.0 0.0 0.028544569817046166 0.0 38-39 0.0 0.0 0.0 0.05166024247169115 0.0 40-41 0.0 0.0 0.0 0.07680460827649659 0.0 42-43 0.0 0.0 0.0 0.11192100097434418 0.0 44-45 0.0 0.0 0.0 0.1512947919732327 0.0 46-47 0.0 0.0 0.0 0.2009549144377234 0.0 48-49 0.0 0.0 0.0 0.2522151329079745 0.0 50-51 0.0 0.0 0.0 0.3046468502395858 0.0 52-53 0.0 0.0 0.0 0.361221673300398 0.0 54-55 0.0 0.0 0.0 0.43216878727009334 0.0 56-57 0.0 0.0 0.0 0.5241743076013132 0.0 58-59 0.0 0.0 0.0 0.6256375382522952 0.0 60-61 0.0 0.0 0.0 0.7299009369133577 0.0 62-63 0.0 0.0 0.0 0.8336785921441001 0.0 64-65 0.0 0.0 0.0 0.9566002531580466 0.0 66-67 0.0 0.0 0.0 1.1066378268410388 0.0 68-69 0.0 0.0 0.0 1.287505821777878 0.0 70-71 0.0 0.0 0.0 1.4663736967075167 0.0 72-73 0.0 0.0 0.0 1.6463844973555557 0.0 74-75 0.0 0.0 0.0 1.8456535963586387 0.0 76-77 0.0 0.0 0.0 2.08409647435989 0.0 78-79 0.0 0.0 0.0 2.36214172850371 0.0 80-81 0.0 0.0 0.0 2.6576165998531343 0.0 82-83 0.0 0.0 0.0 2.962777766666 0.0 84-85 0.0 0.0 0.0 3.2728249409250267 0.0 86-87 0.0 0.0 0.0 3.6265033044839834 0.0 88-89 0.0 0.0 0.0 4.02975607107855 0.0 90-91 0.0 0.0 0.0 4.467468048082885 0.0 92-93 0.0 0.0 0.0 4.9067801210929805 0.0 94-95 0.0 0.0 0.0 5.361893142159959 0.0 96-97 0.0 0.0 0.0 5.864723311970147 0.0 98-99 0.0 0.0 0.0 6.406384383062983 0.0 100-101 0.0 0.0 0.0 7.005963214935753 0.0 102-103 0.0 0.0 0.0 7.6047705719486025 0.0 104-105 0.0 0.0 0.0 8.228407990193697 0.0 106-107 0.0 0.0 0.0 8.874161021089837 0.0 108-109 0.0 0.0 0.0 9.544944125218942 0.0 110-111 0.0 0.0 0.0 10.28281696901814 0.0 112-113 0.0 0.0 0.0 11.029861791707502 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5195 0.0 84.87146 1 GTATCAA 8035 0.0 54.750607 2 GTACATG 13365 0.0 48.013374 1 TACATGG 14175 0.0 45.271626 2 ACATGGG 13855 0.0 45.11419 3 CATGGGA 8995 0.0 43.612865 4 ATCAACG 11515 0.0 39.54831 4 ATGGGAT 3750 0.0 39.52742 5 TCAACGC 11620 0.0 39.2934 5 CAACGCA 11700 0.0 39.154976 6 AACGCAG 12035 0.0 38.114517 7 TATCAAC 12235 0.0 37.366947 3 CATGGGG 4350 0.0 36.94919 4 ATGGGAG 3440 0.0 34.090878 5 ACGCAGA 13780 0.0 32.985744 8 CGCAGAG 14065 0.0 32.19045 9 GTCGGGA 565 0.0 30.554834 2 AGTACAT 8555 0.0 29.225346 2 TACCTGG 1365 0.0 29.219482 2 GAGTACA 8965 0.0 28.976883 1 >>END_MODULE