FastQCFastQC Report
Thu 26 May 2016
SRR936221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326669
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG59330.25499974426959743No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG49940.21464161855425076No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42750.1837390707487829No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41440.17810870390244593No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA40130.172478337056109No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA34180.14690529679984562No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA32420.1393408344719425No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31100.1336674877260152No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG30250.13001419626083469No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28140.12094543744726903No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG27960.12017179925464258No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA27580.11853856307020895No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG26170.11247839722796839No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT23870.10259302032218592No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT23570.10130362333447517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.059.267041
GTACATG118450.051.5825841
TACATGG116650.051.1545072
ACATGGG120650.048.472333
GTATCAA33050.046.2625272
CATGGGG67300.041.3712354
ATCAACG40200.039.6621674
CATGGGA54250.038.711744
TCAACGC41650.038.567055
CAACGCA41350.037.98366
AACGCAG43150.036.6748667
TATCAAC45450.033.6408463
GTACGGT1551.9742583E-730.7061946
ATGGGGA36400.030.2369565
ATGGGGG35000.029.7466285
ACGCAGA51550.029.429258
CGCAGAG51850.028.9147559
ATGGGAG28100.027.100135
GAGTACA67500.026.8821371
ATGGGAT23700.026.8598215
Produced by FastQC (version 0.11.5)