FastQCFastQC Report
Thu 26 May 2016
SRR936221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326669
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG67230.28895386494598074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.20385366375707073No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG46890.20153274917919134No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41210.17712016621186769No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39330.1690399450888803No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35270.1515901058551947No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31750.1364611811993885No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG31330.13465602541659344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.12782222138172641No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29570.12709156308869032No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA29480.12670474399237708No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA28490.12244973393293158No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT27950.12012881935505222No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT27740.11922624146365471No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27480.11810876407430536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.11759300527922106No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG27190.11686234698618497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.079.9765551
GTATCAA69950.054.55332
TACATGG107100.044.74632
ACATGGG108300.043.9206773
GTACATG110350.043.786971
CAACGCA94600.042.3885656
TCAACGC94600.042.2891355
ATCAACG94650.042.20394
AACGCAG98650.040.8895727
TATCAAC98650.040.4322973
TGGTATC14400.037.6209372
CATGGGG60850.036.1986054
CATGGGA49400.035.7915234
ACGCAGA116200.034.2531058
CGCAGAG118050.033.7163129
GTGGTAT18150.031.1848261
ATGGGGG29550.028.6076075
TATACCG1704.4071385E-728.0150745
ATGGGAT21900.026.6399025
ATGGGGA34900.025.9279925
Produced by FastQC (version 0.11.5)