FastQCFastQC Report
Thu 26 May 2016
SRR936220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334953
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.066.826331
GTACATG157400.054.1647531
TACATGG159950.052.6689072
ACATGGG163050.050.7918173
GTATCAA39200.048.566772
CATGGGA100100.046.537494
CATGGGG61750.042.970784
TCAACGC46250.042.9645275
ATCAACG46750.042.377754
CAACGCA48350.041.713686
AACGCAG49000.040.4318357
ATGGGAG41000.037.437875
ATGGGAT45450.034.8195425
TATCAAC56750.033.8619463
GAGTACA93150.032.892711
ACGCAGA60400.032.505168
AGTACAT91900.031.9806122
CGCAGAG61950.031.3077329
ATGGGAC25000.030.2231035
ATGGGGA35650.030.2060785
Produced by FastQC (version 0.11.5)