##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2334953 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.550845777195516 31.0 29.0 31.0 22.0 34.0 2 28.80248596010284 31.0 30.0 33.0 22.0 34.0 3 28.859809597880556 31.0 30.0 34.0 22.0 34.0 4 32.38948535580802 35.0 33.0 37.0 23.0 37.0 5 32.39585935991003 35.0 33.0 37.0 25.0 37.0 6 32.50616479218211 35.0 33.0 37.0 25.0 37.0 7 32.38100124499294 35.0 33.0 37.0 23.0 37.0 8 32.33951090236078 35.0 33.0 37.0 22.0 37.0 9 33.631467528468455 38.0 33.0 39.0 19.0 39.0 10-11 33.435900636972136 37.5 33.0 39.0 18.5 39.0 12-13 33.405213723788016 37.0 33.0 39.0 18.0 39.0 14-15 34.37250428595351 39.0 33.0 41.0 18.5 41.0 16-17 34.33886356599041 38.0 33.0 41.0 18.0 41.0 18-19 34.33035718491978 38.5 33.0 40.5 18.5 41.0 20-21 34.40212972166891 39.0 33.0 41.0 19.0 41.0 22-23 34.260720451332425 39.0 33.0 40.0 19.0 41.0 24-25 34.178444062899764 38.0 33.0 40.0 19.5 41.0 26-27 34.23055453364586 38.0 33.0 40.0 21.0 41.0 28-29 34.10327295667193 38.0 33.0 40.0 19.5 41.0 30-31 34.013285278119085 38.0 33.0 40.0 19.0 41.0 32-33 33.82506221752644 38.0 32.5 40.0 18.5 41.0 34-35 33.78318578575244 38.0 32.0 40.0 18.0 41.0 36-37 33.67876655333105 38.0 32.0 40.0 18.0 41.0 38-39 33.421956244943686 38.0 32.0 40.0 16.0 41.0 40-41 33.50762199496093 38.0 32.0 40.0 17.0 41.0 42-43 33.41262415132125 38.0 32.0 40.0 15.5 41.0 44-45 33.27034441378477 38.0 32.0 40.0 15.0 41.0 46-47 33.2314245725717 38.0 32.0 40.0 15.0 41.0 48-49 33.13460335175911 38.0 32.0 40.0 13.5 41.0 50-51 32.98371680286498 38.0 31.5 40.0 12.0 41.0 52-53 32.81806443213204 38.0 31.0 40.0 9.0 41.0 54-55 32.63554020145159 37.5 31.0 40.0 8.0 41.0 56-57 32.42522697459007 37.0 31.0 40.0 7.5 41.0 58-59 32.18588832409046 37.0 31.0 40.0 6.5 41.0 60-61 31.942835894341343 36.5 30.0 40.0 2.0 41.0 62-63 31.70014235832584 36.0 30.0 40.0 2.0 41.0 64-65 31.414969594677068 36.0 30.0 39.0 2.0 41.0 66-67 31.119888494543574 35.0 30.0 39.0 2.0 40.5 68-69 30.845022576471564 35.0 29.0 39.0 2.0 40.0 70-71 30.519662494277185 35.0 29.0 38.0 2.0 40.0 72-73 30.194775012601966 35.0 29.0 37.5 2.0 40.0 74-75 29.853347797578795 34.0 29.0 37.0 2.0 39.0 76-77 29.537328160352693 34.0 29.0 36.5 2.0 39.0 78-79 29.202794445969577 34.0 29.0 36.0 2.0 39.0 80-81 28.888161988699558 34.0 28.0 36.0 2.0 37.5 82-83 28.584450950404566 34.0 28.0 35.0 2.0 37.0 84-85 28.602409127721202 34.0 29.0 35.0 2.0 37.0 86-87 28.473811036025136 34.0 29.0 35.0 2.0 36.0 88-89 28.29345708457515 34.0 28.5 35.0 2.0 36.0 90-91 28.10835614250051 34.0 28.0 35.0 2.0 36.0 92-93 27.97759890670176 34.0 28.0 35.0 2.0 35.5 94-95 27.842212669805345 34.0 27.5 35.0 2.0 35.0 96-97 27.723381798263176 34.0 27.0 35.0 2.0 35.0 98-99 27.60270592170378 34.0 27.0 35.0 2.0 35.0 100-101 27.483046982101996 34.0 27.0 35.0 2.0 35.0 102-103 26.532041330168102 32.5 25.0 34.0 2.0 35.0 104-105 27.181784815368875 33.5 26.0 35.0 2.0 35.0 106-107 27.300391485396066 34.0 27.0 35.0 2.0 35.0 108-109 27.244319478807498 34.0 26.5 35.0 2.0 35.0 110-111 27.11705203488036 34.0 25.5 35.0 2.0 35.0 112-113 27.068123426895532 34.0 25.5 35.0 2.0 35.0 114-115 26.93885444375112 34.0 25.0 35.0 2.0 35.0 116-117 26.796504255117767 34.0 25.0 35.0 2.0 35.0 118-119 26.56769151242016 34.0 25.0 35.0 2.0 35.0 120-121 26.324126010245173 34.0 25.0 35.0 2.0 35.0 122-123 25.973229011461903 33.5 24.0 35.0 2.0 35.0 124-125 23.97507701439815 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4745095015866454 1101 2 0.5929393302153123 1101 3 0.6668313063205673 1101 4 0.6470748498095773 1101 5 0.49783272061890926 1101 6 0.46653355923236006 1101 7 0.45760434462481214 1101 8 0.4547592438942516 1101 9 0.5832051567040892 1101 10-11 0.678322624710006 1101 12-13 0.6932718975322487 1101 14-15 0.8057903461372788 1101 16-17 0.828980081829144 1101 18-19 0.8250901426881683 1101 20-21 0.6183356268966023 1101 22-23 0.7243246732508553 1101 24-25 0.8748295251095328 1101 26-27 0.8254178875407234 1101 28-29 0.8984383188528255 1101 30-31 0.8626958732689971 1101 32-33 0.9469044135332823 1101 34-35 0.7453939758409405 1101 36-37 0.6959912520353697 1101 38-39 0.8935706310605269 1101 40-41 0.9505501180719946 1101 42-43 1.0614263858003739 1101 44-45 0.9428183527355571 1101 46-47 0.8538510155650485 1101 48-49 0.9399895005587737 1101 50-51 0.9338952332223229 1101 52-53 0.9717286895471773 1101 54-55 0.881534627649998 1101 56-57 1.0020456523729493 1101 58-59 0.9618811245757399 1101 60-61 0.9910577974666275 1101 62-63 0.9497998842412905 1101 64-65 0.9913856798468181 1101 66-67 1.0178907136433715 1101 68-69 0.9773079883161131 1101 70-71 0.9582428452334639 1101 72-73 0.9380293950566383 1101 74-75 0.9234877716149583 1101 76-77 0.9707370747423028 1101 78-79 0.9677229907838836 1101 80-81 0.9440436188812633 1101 82-83 0.8127382906470793 1101 84-85 0.5669071190132158 1101 86-87 0.7237973608134993 1101 88-89 0.6393550333038398 1101 90-91 0.6329112596306175 1101 92-93 0.6382347740144141 1101 94-95 0.8438650089943884 1101 96-97 0.8385728658894642 1101 98-99 0.8762072334721722 1101 100-101 0.9029324027264991 1101 102-103 0.8570820466158224 1101 104-105 0.8213285164190331 1101 106-107 0.8987167627806727 1101 108-109 0.9112026941782254 1101 110-111 0.906710664650177 1101 112-113 0.9454768203063857 1101 114-115 0.9418473311158415 1101 116-117 0.8984891597710742 1101 118-119 0.845678433010093 1101 120-121 1.0251715348844606 1101 122-123 0.9629301152337 1101 124-125 0.84346383891749 1102 1 0.3128549156408944 1102 2 0.38455999069319446 1102 3 0.3926534790567615 1102 4 0.4502998080648055 1102 5 0.38842305046024705 1102 6 0.2785804596426331 1102 7 0.3200816220780851 1102 8 0.2727151310635918 1102 9 0.4945227589560943 1102 10-11 0.5894712090882663 1102 12-13 0.620526372497217 1102 14-15 0.6687624878995635 1102 16-17 0.5548343240653111 1102 18-19 0.5449081359413839 1102 20-21 0.546405552337184 1102 22-23 0.7045591593400218 1102 24-25 0.7360549311768736 1102 26-27 0.5434657822506139 1102 28-29 0.5917763736098323 1102 30-31 0.517965291169844 1102 32-33 0.6252677531320145 1102 34-35 0.5004133241974102 1102 36-37 0.5876581956219269 1102 38-39 0.6330387025357567 1102 40-41 0.5805975272409398 1102 42-43 0.7264200089036521 1102 44-45 0.5383033661938654 1102 46-47 0.43944019395171807 1102 48-49 0.553574409203506 1102 50-51 0.41777932998630973 1102 52-53 0.3633685336806991 1102 54-55 0.4448684799965079 1102 56-57 0.5695271721422017 1102 58-59 0.6738496360996464 1102 60-61 0.6467007307201378 1102 62-63 0.6380114625497164 1102 64-65 0.5426861053263679 1102 66-67 0.5306777737597592 1102 68-69 0.6323815791119074 1102 70-71 0.5159985273031182 1102 72-73 0.5277859033875245 1102 74-75 0.5557385077986261 1102 76-77 0.540169827685407 1102 78-79 0.49253919699686577 1102 80-81 0.5103379504302623 1102 82-83 0.43858528459581336 1102 84-85 0.49432295725407727 1102 86-87 0.4920869521711637 1102 88-89 0.5491411355434401 1102 90-91 0.523854535360261 1102 92-93 0.5516436414198012 1102 94-95 0.4807291459047427 1102 96-97 0.5238399271899468 1102 98-99 0.45259583919333934 1102 100-101 0.555827262798271 1102 102-103 0.4993243547607662 1102 104-105 0.5507976878345744 1102 106-107 0.5302150266017946 1102 108-109 0.4662377652963343 1102 110-111 0.5680649436719492 1102 112-113 0.532153640863605 1102 114-115 0.6097988939815657 1102 116-117 0.6249228659474113 1102 118-119 0.7375377573187905 1102 120-121 0.7231124335531156 1102 122-123 0.6928688653909596 1102 124-125 0.6868527260086168 1103 1 0.21566165578052932 1103 2 0.397409184898013 1103 3 0.36622013064544134 1103 4 0.3032708355431524 1103 5 0.3178064705446886 1103 6 0.33738225800500743 1103 7 0.31410896032624436 1103 8 0.35149808022676154 1103 9 0.4509562940659251 1103 10-11 0.6230722734755858 1103 12-13 0.3312961275149675 1103 14-15 0.341390729562697 1103 16-17 0.1681663470789232 1103 18-19 0.31087688584491247 1103 20-21 0.19450903358817584 1103 22-23 0.41124697929091525 1103 24-25 0.39907025765450044 1103 26-27 0.2749523831043348 1103 28-29 0.3212913573413374 1103 30-31 0.3366552380136909 1103 32-33 0.3913291540088508 1103 34-35 0.3664030446068409 1103 36-37 0.3105866911215287 1103 38-39 0.3461465256296563 1103 40-41 0.40234003147951114 1103 42-43 0.4615084209387348 1103 44-45 0.4448724488323563 1103 46-47 0.3189752587985737 1103 48-49 0.18428133796744106 1103 50-51 0.19668775671912186 1103 52-53 0.3670690966134629 1103 54-55 0.26076724736002177 1103 56-57 0.34680735265466467 1103 58-59 0.2813827592820033 1103 60-61 0.3432782015609881 1103 62-63 0.3434906669082878 1103 64-65 0.32401959135065894 1103 66-67 0.2942039705743902 1103 68-69 0.38727848257745734 1103 70-71 0.349136549983605 1103 72-73 0.2878443223804368 1103 74-75 0.29935481531077457 1103 76-77 0.3652698547199371 1103 78-79 0.29415527106236894 1103 80-81 0.27505269642253083 1103 82-83 0.2512829442932336 1103 84-85 0.2571286007237248 1103 86-87 0.23829991254653748 1103 88-89 0.26157978413435856 1103 90-91 0.22300840889127826 1103 92-93 0.2809079787260913 1103 94-95 0.3225957649608375 1103 96-97 0.3670533481373184 1103 98-99 0.18578830580554495 1103 100-101 0.35519501637858397 1103 102-103 0.2482764542443192 1103 104-105 0.29500940923747265 1103 106-107 0.19068346198448438 1103 108-109 0.2245017469934112 1103 110-111 0.1262226689255428 1103 112-113 0.1947717919867884 1103 114-115 0.11345697197773674 1103 116-117 0.28860938827016014 1103 118-119 0.26976294923971267 1103 120-121 0.2766944282311776 1103 122-123 0.30366347924184467 1103 124-125 0.10729236865884673 1104 1 0.33671430591371276 1104 2 0.4162548767258585 1104 3 0.498775409627779 1104 4 0.4164456045842826 1104 5 0.3958884462194945 1104 6 0.4598325445975959 1104 7 0.35810784868013457 1104 8 0.3682885193651657 1104 9 0.5651369500722652 1104 10-11 0.7473107709201869 1104 12-13 0.5243207391365416 1104 14-15 0.6773765854523504 1104 16-17 0.7506989466004796 1104 18-19 0.6723900612308853 1104 20-21 0.6201220179907594 1104 22-23 0.6376861188697802 1104 24-25 0.632390278066012 1104 26-27 0.530184008848579 1104 28-29 0.6228097942446951 1104 30-31 0.6581470970567338 1104 32-33 0.7970857857681395 1104 34-35 0.7604674889409466 1104 36-37 0.6614098204112082 1104 38-39 0.8352655255241928 1104 40-41 0.6824152144795477 1104 42-43 0.7801618955232001 1104 44-45 0.826659578590899 1104 46-47 0.6828550153527786 1104 48-49 0.75866446104083 1104 50-51 0.7098441207315247 1104 52-53 0.7159255464964076 1104 54-55 0.790358215059122 1104 56-57 0.833998745270506 1104 58-59 0.79771931542939 1104 60-61 0.6790464433240366 1104 62-63 0.7068533053168942 1104 64-65 0.735278195664872 1104 66-67 0.6749490789936701 1104 68-69 0.6254220012605423 1104 70-71 0.6652174334014802 1104 72-73 0.6599865854674718 1104 74-75 0.6292294084685643 1104 76-77 0.603945694425505 1104 78-79 0.6046461961934142 1104 80-81 0.5380917706069894 1104 82-83 0.5386973711917804 1104 84-85 0.6332296341961978 1104 86-87 0.6447168045144522 1104 88-89 0.5933983625493262 1104 90-91 0.6848555775280545 1104 92-93 0.6512030555498285 1104 94-95 0.7249458506404345 1104 96-97 0.627800900767916 1104 98-99 0.6280871453045904 1104 100-101 0.6219117913357586 1104 102-103 0.715172373439632 1104 104-105 0.7252054367477747 1104 106-107 0.7916998220426663 1104 108-109 0.7148598915197475 1104 110-111 0.7484526239690901 1104 112-113 0.6713186736931291 1104 114-115 0.6406685058349133 1104 116-117 0.6476618274726036 1104 118-119 0.6224895962253854 1104 120-121 0.7029998412485376 1104 122-123 0.7841078791629918 1104 124-125 0.8450204154842993 1105 1 0.3742672045008355 1105 2 0.46511906780089873 1105 3 0.5294496851330521 1105 4 0.4895882275184462 1105 5 0.5552523136527157 1105 6 0.45395175154848033 1105 7 0.5059662957746767 1105 8 0.4196486092836409 1105 9 0.5022431757159467 1105 10-11 0.6064280108328646 1105 12-13 0.6750575090694966 1105 14-15 0.6911103616805434 1105 16-17 0.6445940514445425 1105 18-19 0.5377893370688867 1105 20-21 0.31871520286529886 1105 22-23 0.22811248888790914 1105 24-25 0.43553147133764014 1105 26-27 0.32896802290306937 1105 28-29 0.4677748061521072 1105 30-31 0.5168547579728298 1105 32-33 0.6608921470651907 1105 34-35 0.7318488363241897 1105 36-37 0.661820615837371 1105 38-39 0.7242414964603938 1105 40-41 0.5971158447188714 1105 42-43 0.7630049433347494 1105 44-45 0.7787411257404955 1105 46-47 0.647959800743827 1105 48-49 0.632097681847263 1105 50-51 0.5832229599800698 1105 52-53 0.6694931412702232 1105 54-55 0.6120369723203112 1105 56-57 0.6689660655319472 1105 58-59 0.6684708814393687 1105 60-61 0.7412714318502474 1105 62-63 0.7125889496947089 1105 64-65 0.6629678610079175 1105 66-67 0.769078117557779 1105 68-69 0.6775182099643686 1105 70-71 0.5567355632826718 1105 72-73 0.4871361632304527 1105 74-75 0.5272575474901551 1105 76-77 0.4715345077383368 1105 78-79 0.4375016873640689 1105 80-81 0.5681638776905729 1105 82-83 0.5537666439944324 1105 84-85 0.6542799839340141 1105 86-87 0.6825708052010881 1105 88-89 0.5774426703529549 1105 90-91 0.5373832752719174 1105 92-93 0.5153817158544243 1105 94-95 0.6028066962478995 1105 96-97 0.5856711858689536 1105 98-99 0.5273958984599716 1105 100-101 0.43793898096983597 1105 102-103 0.5036934426440283 1105 104-105 0.5303475851312918 1105 106-107 0.5281607818800254 1105 108-109 0.5393629120575127 1105 110-111 0.6004293506658414 1105 112-113 0.5066256282500348 1105 114-115 0.4512988625078087 1105 116-117 0.44569544758278923 1105 118-119 0.5782469431380832 1105 120-121 0.6058107309551559 1105 122-123 0.7426312080642319 1105 124-125 0.6733305028482697 1106 1 -0.4156519848807321 1106 2 -0.4389714987108455 1106 3 -0.3334058327387943 1106 4 -0.3217156985656615 1106 5 -0.36379840582697653 1106 6 -0.3837123470432644 1106 7 -0.47678294008346356 1106 8 -0.4779065554779045 1106 9 -0.475430692726043 1106 10-11 -0.3352672675380788 1106 12-13 -0.43190288116623776 1106 14-15 -0.4285960194273599 1106 16-17 -0.3589546024096677 1106 18-19 -0.5853587529253943 1106 20-21 -0.5590556313403354 1106 22-23 -0.6128227998111626 1106 24-25 -4.424916720542214 1106 26-27 -1.5175481182852266 1106 28-29 -1.1688415108934862 1106 30-31 -1.0775754155117951 1106 32-33 -5.876882246157326 1106 34-35 -1.6621927064747268 1106 36-37 -0.9366504374920481 1106 38-39 -0.32657508855823636 1106 40-41 -1.0038212583809525 1106 42-43 -0.5845676326956166 1106 44-45 -0.5627608491649525 1106 46-47 -0.6724241194783644 1106 48-49 -0.4206640105699293 1106 50-51 -0.39098952030577294 1106 52-53 -0.36984198552848113 1106 54-55 -0.48321564809309336 1106 56-57 -0.350273700549927 1106 58-59 -0.2784819371624323 1106 60-61 -0.32819387820293144 1106 62-63 -0.27414614081597577 1106 64-65 -0.35534987545734964 1106 66-67 -0.3351610553546145 1106 68-69 -0.3237449967796202 1106 70-71 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-2.086257635457354 2216 68-69 -2.0018288144799 2216 70-71 -1.9766736974408623 2216 72-73 -2.1567587345617696 2216 74-75 -2.0007852104312427 2216 76-77 -1.9170174634318222 2216 78-79 -1.712814394653634 2216 80-81 -1.9106232024015384 2216 82-83 -1.8860709745615125 2216 84-85 -2.063124234308063 2216 86-87 -1.8200266047237967 2216 88-89 -1.8312848688016423 2216 90-91 -1.805036358164827 2216 92-93 -1.6582127206506918 2216 94-95 -1.818076882559815 2216 96-97 -1.8485895578164566 2216 98-99 -1.715435129243854 2216 100-101 -1.8256511755518225 2216 102-103 -1.720827154877128 2216 104-105 -1.8230621753266583 2216 106-107 -1.8773035721447329 2216 108-109 -1.8032715265755037 2216 110-111 -1.8508727395942515 2216 112-113 -1.955546894578557 2216 114-115 -1.9300371855118321 2216 116-117 -1.8522558831212947 2216 118-119 -1.9823861558842921 2216 120-121 -2.0564494066007732 2216 122-123 -1.9893088334782085 2216 124-125 -1.926198860175738 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103919.0 3 17361.0 4 10904.0 5 8937.0 6 9226.0 7 10548.0 8 11414.0 9 12626.0 10 13964.0 11 15275.0 12 16023.0 13 16142.0 14 16006.0 15 15308.0 16 14166.0 17 13355.0 18 11857.0 19 11117.0 20 11287.0 21 11819.0 22 13866.0 23 18200.0 24 25477.0 25 34478.0 26 40239.0 27 42293.0 28 48476.0 29 57872.0 30 68307.0 31 81234.0 32 100657.0 33 128114.0 34 169388.0 35 233870.0 36 356497.0 37 433922.0 38 130509.0 39 300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.74408412696305 17.892997142975837 9.37100499497419 22.99191373508692 2 19.155340238257573 20.48376155055864 37.257896522069835 23.103001689113952 3 17.322041157198225 27.44812522752093 30.493843551253775 24.735990064027067 4 10.99344481628269 16.039000473672623 37.44026628453078 35.52728842551391 5 11.132210904281745 41.93369754031802 35.20379145295631 11.730300102443922 6 31.482468102018718 37.3500606438527 18.56725471788617 12.600216536242412 7 28.12119903928844 34.09245478248654 21.909979545477906 15.876366632747107 8 26.766875366981495 34.44634457522655 19.88344817632414 18.903331881467814 9 27.38579704161424 13.289822957683384 18.874163676559647 40.45021632414274 10-11 24.056938922611153 26.629935976475455 29.5552572825742 19.757867818339193 12-13 24.797347909750815 25.785414702817622 28.612200968515744 20.805036418915822 14-15 22.54325939361364 27.27822099257034 25.42133005838586 24.757189555430166 16-17 22.686317161783595 29.431608079650417 26.047313314514565 21.834761444051423 18-19 21.77746179979678 28.462066403200993 27.637770742033123 22.122701054969106 20-21 23.075353249138292 27.84644369599331 27.23382540378672 21.844377651081675 22-23 22.876583235476733 27.991974008544222 26.984395739160423 22.147047016818618 24-25 22.56113412654123 27.98394111628429 27.186650260588536 22.268274496585946 26-27 22.385587499344194 28.04006993823122 27.152843730024724 22.421498832399863 28-29 22.348728919268197 27.98848784060285 27.250497607522306 22.41228563260665 30-31 22.49855456052216 27.707832782995634 27.33767179315868 22.455940863323526 32-33 22.19443868644525 28.063386434458454 27.30537201338292 22.43680286571337 34-35 22.761397701476294 27.92458543251874 27.17584890533285 22.138167960672114 36-37 22.702534864423566 27.628726678979632 27.023871088626567 22.644867367970235 38-39 22.29347810259305 27.93395873288067 27.350965808883675 22.4215973556426 40-41 22.522547794815175 27.815064183964484 27.43372496551287 22.228663055707475 42-43 22.305163157065227 27.693487764933224 27.38801796029685 22.6133311177047 44-45 22.21204584807504 27.98701802067643 27.33430010077394 22.46663603047459 46-47 22.462761036780705 27.980508968187344 26.964649752454044 22.592080242577907 48-49 22.388976019145627 27.91642718932258 27.165960154967184 22.528636636564613 50-51 22.351043898849834 27.76134731551277 27.272637723403903 22.61497106223349 52-53 22.587642001349078 27.686735119971733 27.244750902063235 22.48087197661595 54-55 22.325423230848266 27.79379898844738 27.334812181373668 22.545965599330685 56-57 22.460468819394507 28.00301422441539 27.186651071617167 22.349865884572935 58-59 22.671156725494832 27.539662757351806 27.168389197819554 22.620791319333804 60-61 22.670100595969608 27.802278821954108 27.36233072062621 22.165289861450074 62-63 22.242270425469222 27.83214300398128 27.395341526590972 22.53024504395853 64-65 22.116863410524765 27.79210606223482 27.386184002867754 22.70484652437267 66-67 22.23996474432138 28.21801217417299 27.01299346493683 22.5290296165688 68-69 22.35936303506545 27.816144085435734 27.274461786729297 22.55003109276952 70-71 22.206102379866905 27.76699116444673 27.38933716326512 22.637569292421244 72-73 22.402257007644703 27.91505171416947 27.089380928927813 22.593310349258015 74-75 22.02589609100335 28.059245027831466 27.334945932391612 22.57991294877357 76-77 22.00268357268923 27.739280591807862 27.509939197763376 22.74809663773953 78-79 22.288076650957535 27.938609339479935 27.184759574945566 22.58855443461696 80-81 22.12339522416914 27.88633734120875 27.098759734974433 22.89150769964768 82-83 22.388523772579994 27.89768168334334 27.10445219669727 22.609342347379396 84-85 22.128798695986973 27.704730644734397 27.274014045720975 22.89245661355766 86-87 21.926112653973014 27.765668292613988 27.50446316435944 22.803755889053562 88-89 22.180972142812784 27.665191257181647 27.391287589215164 22.762549010790405 90-91 22.138001877130804 27.712776288450293 27.543050772255384 22.606171062163522 92-93 22.14230604427125 27.719842831516694 27.386580377751695 22.75127074646036 94-95 21.953702987928413 27.519614483507215 27.521113447829936 23.005569080734432 96-97 22.24849932161114 27.52365795496594 27.54511457234092 22.682728151081996 98-99 21.880165408713314 27.7151459981794 27.3171920434049 23.08749654970238 100-101 21.83511078533592 27.583622473091964 27.594350784076788 22.98691595749533 102-103 21.86809871193444 27.401870415692425 27.592475252528388 23.137555619844747 104-105 21.62431339345042 27.373895770925504 27.749365241600067 23.25242559402401 106-107 21.618626582881593 27.51639494334443 27.733016980075526 23.13196149369845 108-109 21.735775910157805 27.704220046909157 27.288705890968863 23.271298151964174 110-111 21.607568342865 27.727670538363675 27.41995339059542 23.244807728175907 112-113 21.887972069571678 27.443050573578944 27.56555883876469 23.10341851808469 114-115 21.476099324844768 27.815971643009075 27.397246487015003 23.310682545131158 116-117 21.315469618465244 27.670456346164222 27.694825301571175 23.319248733799355 118-119 21.467307685306984 27.535092601296956 27.504749072723612 23.49285064067245 120-121 21.41625848361907 27.49424931936182 27.577955931220533 23.51153626579858 122-123 21.53441695907259 27.554702713861047 27.42200117433475 23.488879152731613 124-125 21.573527427840915 27.301242641689633 27.214302692354636 23.910927238114816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 41.0 2 65.5 3 79.0 4 88.5 5 96.5 6 100.0 7 106.0 8 121.5 9 144.5 10 159.5 11 175.5 12 201.5 13 231.5 14 282.5 15 371.5 16 446.0 17 545.0 18 756.5 19 1034.0 20 1461.5 21 2235.5 22 3384.0 23 5032.0 24 7050.0 25 9701.5 26 13748.0 27 17906.0 28 20760.0 29 24615.0 30 31080.0 31 37082.5 32 42300.0 33 47647.5 34 54322.0 35 59471.5 36 64485.5 37 70370.5 38 72263.0 39 73157.0 40 73467.0 41 75237.5 42 78461.5 43 81217.5 44 84896.5 45 87594.5 46 87382.5 47 86407.0 48 86710.5 49 86012.5 50 83947.0 51 80641.0 52 77097.0 53 74427.5 54 71426.5 55 66727.5 56 60366.0 57 54151.5 58 49393.0 59 44723.0 60 38012.5 61 30826.5 62 25000.5 63 19899.5 64 15454.0 65 12128.5 66 9276.0 67 7329.5 68 5927.0 69 4253.0 70 3072.0 71 2436.5 72 1999.0 73 1563.0 74 1230.0 75 983.0 76 652.0 77 436.5 78 330.5 79 232.5 80 136.5 81 87.5 82 64.5 83 47.5 84 32.0 85 26.5 86 20.0 87 16.0 88 9.5 89 5.5 90 6.0 91 6.0 92 5.5 93 4.5 94 4.5 95 4.0 96 3.5 97 4.0 98 3.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.569644870796115E-4 2 4.2827414513268574E-5 3 1.2848224353980575E-4 4 2.9979190159288004E-4 5 7.280660467255658E-4 6 3.854467306194172E-4 7 7.280660467255658E-4 8 0.001113512777344983 9 0.0013276498499113258 10-11 0.0012848224353980574 12-13 0.001713096580530743 14-15 0.002376921505486406 16-17 0.0021413707256634287 18-19 0.0021627844329200627 20-21 0.002441162627256309 22-23 0.08704671999821838 24-25 0.0019700610676103543 26-27 0.0015203732152210343 28-29 0.0013062361426546916 30-31 9.850305338051772E-4 32-33 0.0015846143369909374 34-35 8.351345830087373E-4 36-37 6.638249249556629E-4 38-39 8.137208757521029E-4 40-41 0.001027857948318446 42-43 0.0010706853628317144 44-45 0.001027857948318446 46-47 0.001413304678937863 48-49 2.9979190159288004E-4 50-51 0.0012205813136281546 52-53 0.0011991676063715202 54-55 0.0011777538991148858 56-57 0.001027857948318446 58-59 7.280660467255658E-4 60-61 7.923071684954687E-4 62-63 0.0010706853628317144 64-65 9.422031192919086E-4 66-67 4.2827414513268574E-4 68-69 2.1413707256634287E-4 70-71 4.925152669025886E-4 72-73 1.2848224353980575E-4 74-75 2.569644870796115E-4 76-77 2.569644870796115E-4 78-79 2.1413707256634287E-4 80-81 3.640330233627829E-4 82-83 1.927233653097086E-4 84-85 2.9979190159288004E-4 86-87 2.7837819433624574E-4 88-89 3.426193161061486E-4 90-91 2.7837819433624574E-4 92-93 2.7837819433624574E-4 94-95 3.2120560884951434E-4 96-97 3.854467306194172E-4 98-99 4.0686043787605144E-4 100-101 4.0686043787605144E-4 102-103 7.494797539822001E-4 104-105 4.0686043787605144E-4 106-107 4.496878523893201E-4 108-109 6.209975104423944E-4 110-111 7.066523394689315E-4 112-113 3.640330233627829E-4 114-115 3.640330233627829E-4 116-117 6.424112176990287E-4 118-119 0.0010064442410618116 120-121 5.13928974159223E-4 122-123 5.995838031857601E-4 124-125 3.426193161061486E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2334953.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.76547860761985 #Duplication Level Percentage of deduplicated Percentage of total 1 56.06472470001663 19.491169872005244 2 14.316323638633548 9.95427686397354 3 9.711989122827324 10.1292585026127 4 6.4787409023376386 9.009461129781235 5 4.070954277281034 7.076433691970617 6 2.563670283190045 5.347633463234054 7 1.621484012803057 3.9460167431791584 8 1.092153293955846 3.0375385581810814 9 0.774641601478419 2.423770742229332 >10 3.0021766254378535 18.05196653020486 >50 0.19810977391456971 4.759476587183601 >100 0.10191558172702984 6.1033959496870605 >500 0.0031161863969105205 0.6696013657575471 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.2827414513268574E-5 7 0.0 0.0 0.0 0.0 4.2827414513268574E-5 8 0.0 0.0 0.0 0.0 4.2827414513268574E-5 9 0.0 0.0 0.0 4.2827414513268574E-5 4.2827414513268574E-5 10-11 0.0 0.0 0.0 4.2827414513268574E-5 4.2827414513268574E-5 12-13 0.0 0.0 0.0 4.2827414513268574E-5 4.2827414513268574E-5 14-15 0.0 0.0 0.0 6.424112176990286E-5 4.2827414513268574E-5 16-17 0.0 0.0 0.0 8.565482902653715E-5 4.2827414513268574E-5 18-19 0.0 0.0 0.0 8.565482902653715E-5 4.2827414513268574E-5 20-21 0.0 0.0 0.0 8.565482902653715E-5 4.2827414513268574E-5 22-23 0.0 0.0 0.0 1.4989595079644E-4 4.2827414513268574E-5 24-25 0.0 0.0 0.0 2.7837819433624574E-4 4.2827414513268574E-5 26-27 0.0 0.0 0.0 3.854467306194172E-4 4.2827414513268574E-5 28-29 0.0 0.0 0.0 7.280660467255658E-4 4.2827414513268574E-5 30-31 2.1413707256634287E-5 0.0 0.0 0.002376921505486406 4.2827414513268574E-5 32-33 4.2827414513268574E-5 0.0 0.0 0.006509767006016824 4.2827414513268574E-5 34-35 4.2827414513268574E-5 0.0 0.0 0.012355709087077983 4.2827414513268574E-5 36-37 4.2827414513268574E-5 0.0 0.0 0.024390212565306456 4.2827414513268574E-5 38-39 4.2827414513268574E-5 0.0 0.0 0.04409082324141 4.2827414513268574E-5 40-41 4.2827414513268574E-5 0.0 0.0 0.0642625354771595 4.2827414513268574E-5 42-43 4.2827414513268574E-5 0.0 0.0 0.08955212374724458 4.2827414513268574E-5 44-45 4.2827414513268574E-5 0.0 0.0 0.1240281924304258 4.2827414513268574E-5 46-47 4.2827414513268574E-5 0.0 0.0 0.16458575397449116 4.2827414513268574E-5 48-49 4.2827414513268574E-5 0.0 0.0 0.20874081833767102 4.2827414513268574E-5 50-51 4.2827414513268574E-5 0.0 0.0 0.25086158051147067 4.2827414513268574E-5 52-53 4.2827414513268574E-5 0.0 0.0 0.2993208000332341 1.4989595079644E-4 54-55 4.2827414513268574E-5 0.0 0.0 0.36007148752030554 2.5696448707961144E-4 56-57 4.2827414513268574E-5 0.0 0.0 0.4357903563797644 2.5696448707961144E-4 58-59 4.2827414513268574E-5 0.0 0.0 0.5257279268576284 2.5696448707961144E-4 60-61 4.2827414513268574E-5 0.0 0.0 0.6112328599333691 2.5696448707961144E-4 62-63 4.2827414513268574E-5 4.2827414513268574E-5 0.0 0.6983009936388441 2.5696448707961144E-4 64-65 4.2827414513268574E-5 4.2827414513268574E-5 0.0 0.8038063292922812 2.9979190159288004E-4 66-67 4.2827414513268574E-5 4.2827414513268574E-5 0.0 0.9310037503966889 2.9979190159288004E-4 68-69 4.2827414513268574E-5 4.2827414513268574E-5 0.0 1.0894223566812693 2.9979190159288004E-4 70-71 4.2827414513268574E-5 4.2827414513268574E-5 0.0 1.248012272623903 2.9979190159288004E-4 72-73 4.2827414513268574E-5 4.2827414513268574E-5 0.0 1.4076086328075983 4.7110155964595434E-4 74-75 4.2827414513268574E-5 4.2827414513268574E-5 0.0 1.5801602858815573 4.7110155964595434E-4 76-77 4.2827414513268574E-5 4.2827414513268574E-5 0.0 1.7901002718256 4.7110155964595434E-4 78-79 4.2827414513268574E-5 4.2827414513268574E-5 0.0 2.0364007326914075 4.7110155964595434E-4 80-81 4.2827414513268574E-5 4.2827414513268574E-5 0.0 2.2945429736701337 4.7110155964595434E-4 82-83 4.2827414513268574E-5 4.2827414513268574E-5 0.0 2.555511824006736 5.139289741592229E-4 84-85 4.2827414513268574E-5 4.2827414513268574E-5 0.0 2.829414553526345 5.139289741592229E-4 86-87 4.2827414513268574E-5 4.2827414513268574E-5 0.0 3.136829734902587 5.139289741592229E-4 88-89 4.2827414513268574E-5 4.2827414513268574E-5 0.0 3.486023059136522 5.139289741592229E-4 90-91 4.2827414513268574E-5 4.2827414513268574E-5 0.0 3.858321773500366 5.139289741592229E-4 92-93 4.2827414513268574E-5 4.2827414513268574E-5 0.0 4.242483681684385 5.139289741592229E-4 94-95 4.2827414513268574E-5 4.2827414513268574E-5 0.0 4.64264162918911 5.139289741592229E-4 96-97 4.2827414513268574E-5 4.2827414513268574E-5 0.0 5.080830320781617 5.139289741592229E-4 98-99 4.2827414513268574E-5 4.2827414513268574E-5 0.0 5.562167632496243 5.139289741592229E-4 100-101 4.2827414513268574E-5 4.2827414513268574E-5 0.0 6.087338802965199 5.139289741592229E-4 102-103 4.2827414513268574E-5 4.2827414513268574E-5 0.0 6.610454257537517 5.139289741592229E-4 104-105 4.2827414513268574E-5 4.2827414513268574E-5 0.0 7.152135396301339 5.139289741592229E-4 106-107 4.2827414513268574E-5 4.2827414513268574E-5 0.0 7.721483044840731 5.139289741592229E-4 108-109 4.2827414513268574E-5 4.2827414513268574E-5 0.0 8.324343145236757 5.9958380318576E-4 110-111 4.2827414513268574E-5 4.2827414513268574E-5 0.0 8.967161223373662 6.852386322122972E-4 112-113 4.2827414513268574E-5 4.2827414513268574E-5 0.0 9.608009240442955 6.852386322122972E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2840 0.0 66.82633 1 GTACATG 15740 0.0 54.164753 1 TACATGG 15995 0.0 52.668907 2 ACATGGG 16305 0.0 50.791817 3 GTATCAA 3920 0.0 48.56677 2 CATGGGA 10010 0.0 46.53749 4 CATGGGG 6175 0.0 42.97078 4 TCAACGC 4625 0.0 42.964527 5 ATCAACG 4675 0.0 42.37775 4 CAACGCA 4835 0.0 41.71368 6 AACGCAG 4900 0.0 40.431835 7 ATGGGAG 4100 0.0 37.43787 5 ATGGGAT 4545 0.0 34.819542 5 TATCAAC 5675 0.0 33.861946 3 GAGTACA 9315 0.0 32.89271 1 ACGCAGA 6040 0.0 32.50516 8 AGTACAT 9190 0.0 31.980612 2 CGCAGAG 6195 0.0 31.307732 9 ATGGGAC 2500 0.0 30.223103 5 ATGGGGA 3565 0.0 30.206078 5 >>END_MODULE