FastQCFastQC Report
Thu 26 May 2016
SRR936220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334953
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43780.18749842073908982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28000.11991676063715202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24340.10424192692529571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43650.079.016211
GTATCAA66550.051.437642
GTACATG137650.047.516891
TACATGG143600.045.479272
ACATGGG143000.044.9207153
CATGGGA89350.040.377494
TCAACGC89150.039.867065
CAACGCA89850.039.795926
ATCAACG89550.039.7554664
AACGCAG93400.038.4107367
TATCAAC100100.035.9223023
CATGGGG55550.034.7233964
ACGCAGA108550.032.4469648
GAGTACA88500.032.10681
CGCAGAG110750.031.8561429
AGTACAT87200.031.2004592
ATGGGAT41550.029.802565
ATGGGAG37700.029.5298715
ATGGGGG34700.026.42125
CAGAGTA122900.025.2697439
Produced by FastQC (version 0.11.5)