FastQCFastQC Report
Thu 26 May 2016
SRR936219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284313
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30550.068.936931
GTACATG160400.050.9615021
TACATGG161500.049.9513242
ACATGGG161050.048.2080043
GTATCAA46850.044.825482
CATGGGA120000.042.9840354
TCAACGC55800.038.7027475
ATCAACG56000.038.670764
CAACGCA56050.038.530126
ATGGGAT52750.037.5570225
AACGCAG57050.037.437617
CATGGGG43750.036.1720354
ATGGGAG45000.034.7706345
TATCAAC64550.032.5347753
GAGTACA98750.031.6889971
CGCAGAG68250.030.2486279
ACGCAGA69400.030.1753658
AGTACAT99050.029.4307582
CATGGGC23850.027.4393394
ATGGGGG23650.025.9104795
Produced by FastQC (version 0.11.5)