FastQCFastQC Report
Thu 26 May 2016
SRR936219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284313
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88250.38633059480027476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64660.2830610341052211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.2806533080186472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59850.084.404071
GTATCAA91250.055.3222352
GTACATG145550.044.9797631
TACATGG147000.044.1008832
ACATGGG146200.043.8128623
ATCAACG124900.041.7997674
TCAACGC125300.041.713845
CAACGCA126700.041.2754866
AACGCAG128950.040.5552867
CATGGGA107850.040.2387164
TATCAAC133600.039.3005683
ACGCAGA151000.034.3179368
ATGGGAT48900.034.208515
CGCAGAG152850.033.5911879
ATGGGAG41250.032.1823435
GAGTACA95550.030.2996251
GTCGGGA5800.029.7650592
AGTACAT94700.029.545042
CATGGGG42500.028.4343854
CGGGAGT6200.027.844734
Produced by FastQC (version 0.11.5)