FastQCFastQC Report
Thu 26 May 2016
SRR936218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538794
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7970.1479229538562048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.071.0697251
GTACATG64100.055.410141
TACATGG64800.053.893452
GTATCAA15250.051.4964182
ACATGGG65050.051.0340273
CATGGGA48700.049.3543364
ATGGGAT18950.049.290525
CATGGGG14750.044.3683854
ATGGGAG15800.042.926025
TCAACGC18300.041.938375
ATCAACG18950.040.813814
CAACGCA18900.040.292216
AACGCAG19450.039.4587257
GTCGGGA2800.038.246122
ATGGGGG7000.035.696385
GGATTCG1001.0618791E-535.696388
TGGGATA6050.035.4013676
AGTACAT35950.033.5946462
TGGGATC7000.033.146646
GAGTACA37400.032.7694051
Produced by FastQC (version 0.11.5)