##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936218_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538794 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.478147492362574 31.0 30.0 33.0 25.0 34.0 2 29.59407491545934 31.0 30.0 34.0 25.0 34.0 3 29.757133524129816 31.0 30.0 34.0 25.0 34.0 4 33.09597916829066 35.0 35.0 37.0 25.0 37.0 5 33.49339450699154 37.0 35.0 37.0 28.0 37.0 6 33.354745969702705 37.0 35.0 37.0 27.0 37.0 7 33.30398259817296 37.0 35.0 37.0 27.0 37.0 8 33.07879449288596 37.0 35.0 37.0 26.0 37.0 9 34.39644094032228 39.0 35.0 39.0 23.0 39.0 10-11 34.37195941305954 39.0 35.0 39.0 25.0 39.0 12-13 34.39197541175292 39.0 35.0 39.0 25.0 39.0 14-15 35.51565069395724 40.0 36.0 41.0 25.0 41.0 16-17 35.580931673329694 40.0 36.0 41.0 25.0 41.0 18-19 35.688452358415276 40.0 36.0 41.0 25.5 41.0 20-21 35.87509697583863 40.0 36.0 41.0 26.5 41.0 22-23 35.82226787974625 40.0 35.5 41.0 27.0 41.0 24-25 35.85453253005787 39.0 35.0 41.0 27.0 41.0 26-27 36.00098739035698 39.0 35.0 41.0 27.5 41.0 28-29 35.91411281491628 39.0 35.0 41.0 27.5 41.0 30-31 35.84935337067599 39.0 35.0 41.0 27.0 41.0 32-33 35.67835759121297 39.0 35.0 41.0 27.0 41.0 34-35 35.63993010315631 39.0 35.0 41.0 27.0 41.0 36-37 35.517864898272805 39.0 35.0 41.0 27.0 41.0 38-39 35.28082346870975 39.0 34.0 41.0 26.0 41.0 40-41 35.397355946799706 39.0 34.5 41.0 26.5 41.0 42-43 35.315263347401796 39.0 34.0 41.0 26.0 41.0 44-45 35.23221769358976 39.0 34.0 41.0 26.0 41.0 46-47 35.236102295125775 39.0 34.0 41.0 26.5 41.0 48-49 35.154913566223826 39.0 34.0 41.0 26.0 41.0 50-51 35.03449184660557 39.0 34.0 40.5 26.0 41.0 52-53 34.900444325660644 39.0 33.5 40.0 26.0 41.0 54-55 34.73989409681622 38.0 33.0 40.0 26.0 41.0 56-57 34.56051941929569 38.0 33.0 40.0 26.0 41.0 58-59 34.33393653232961 38.0 33.0 40.0 25.0 41.0 60-61 34.09052532136587 38.0 33.0 40.0 24.5 41.0 62-63 33.85008092146535 37.0 32.5 40.0 23.5 41.0 64-65 33.5441996384518 37.0 32.0 40.0 23.0 41.0 66-67 33.23644472655597 36.5 32.0 39.5 22.0 41.0 68-69 32.93563588310189 36.0 32.0 39.0 22.0 41.0 70-71 32.57485421144259 35.5 31.0 39.0 20.5 40.5 72-73 32.243108683467156 35.0 31.0 38.5 20.0 40.0 74-75 31.8744213929628 35.0 31.0 37.5 19.0 40.0 76-77 31.54362149541383 35.0 31.0 37.0 18.0 39.0 78-79 31.200813297846672 35.0 31.0 36.5 17.5 39.0 80-81 30.88575411010516 35.0 30.5 36.0 14.5 38.5 82-83 30.566655345085508 34.5 30.0 36.0 11.5 37.0 84-85 30.561671065379347 35.0 31.0 35.5 11.5 37.0 86-87 30.42494998088323 35.0 31.0 35.0 8.5 37.0 88-89 30.246845733248698 35.0 31.0 35.0 7.0 36.0 90-91 30.055087844333826 34.0 31.0 35.0 4.5 36.0 92-93 29.922217025430868 34.0 30.0 35.0 2.0 36.0 94-95 29.798444303388678 34.0 30.0 35.0 2.0 35.5 96-97 29.691830272794427 34.0 30.0 35.0 2.0 35.0 98-99 29.584889586743728 34.0 30.0 35.0 2.0 35.0 100-101 29.473431404210146 34.0 30.0 35.0 2.0 35.0 102-103 28.523312249208416 33.5 28.5 34.5 2.0 35.0 104-105 29.162400657765303 34.0 29.5 35.0 2.0 35.0 106-107 29.282688745605927 34.0 30.0 35.0 2.0 35.0 108-109 29.231582942645986 34.0 30.0 35.0 2.0 35.0 110-111 29.11096077536127 34.0 30.0 35.0 2.0 35.0 112-113 29.072337479630434 34.0 30.0 35.0 2.0 35.0 114-115 28.96125792046682 34.0 29.5 35.0 2.0 35.0 116-117 28.83764667015594 34.0 29.0 35.0 2.0 35.0 118-119 28.63661993266443 34.0 29.0 35.0 2.0 35.0 120-121 28.439627204460333 34.0 29.0 35.0 2.0 35.0 122-123 28.158667319977578 34.0 29.0 35.0 2.0 35.0 124-125 26.17124912304146 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8545613477466425 1101 2 0.9044106683522379 1101 3 0.8968704849233262 1101 4 0.8328112853040608 1101 5 0.7421755060098576 1101 6 0.7680997861954566 1101 7 0.8343213246659928 1101 8 0.9445147813753394 1101 9 1.0041272400739771 1101 10-11 1.0147557627014194 1101 12-13 1.0409926615172296 1101 14-15 1.0859365938782943 1101 16-17 0.9359624492848226 1101 18-19 0.9666742382934146 1101 20-21 0.9806199492442715 1101 22-23 0.950740027586022 1101 24-25 0.8295749269588484 1101 26-27 0.9653589486396328 1101 28-29 0.8974883223909274 1101 30-31 0.7909877068007205 1101 32-33 1.1410624079723064 1101 34-35 1.0476230336441645 1101 36-37 0.8370508766056659 1101 38-39 0.8944271272778579 1101 40-41 0.9046884708507932 1101 42-43 1.0239245953542166 1101 44-45 1.0707336974514234 1101 46-47 1.037146180210101 1101 48-49 1.1530684405072833 1101 50-51 1.1174321567439023 1101 52-53 1.2296574687117143 1101 54-55 1.2052431451805319 1101 56-57 1.1810362408581838 1101 58-59 1.2232435834038569 1101 60-61 1.1738558152210459 1101 62-63 1.2397815972836383 1101 64-65 1.232532968934791 1101 66-67 1.2198965813185296 1101 68-69 1.1042091375520044 1101 70-71 0.9895693280547277 1101 72-73 1.046684558246966 1101 74-75 1.0464205407869756 1101 76-77 0.9856728612282417 1101 78-79 0.8939361670670856 1101 80-81 0.9091207117821511 1101 82-83 0.8287207925616542 1101 84-85 0.7006399561188061 1101 86-87 0.7650408852056252 1101 88-89 0.7993849097162169 1101 90-91 0.6376348858365262 1101 92-93 0.5439288652131253 1101 94-95 0.6983341451671592 1101 96-97 0.6349876610525236 1101 98-99 0.5775528826266125 1101 100-101 0.6820459146380493 1101 102-103 0.8666047133598092 1101 104-105 0.7891023163940183 1101 106-107 0.6565547853550058 1101 108-109 0.505079186881698 1101 110-111 0.6955083008432297 1101 112-113 0.6207531580117163 1101 114-115 0.6259788495026264 1101 116-117 0.5448809687574254 1101 118-119 0.24140428594586538 1101 120-121 0.28851488219143206 1101 122-123 0.7078288981922256 1101 124-125 0.46554032462207573 1102 1 0.2194962004325589 1102 2 0.42989617363841504 1102 3 0.3898203733388286 1102 4 0.3248186228302714 1102 5 0.15444923744062322 1102 6 0.34023662936851196 1102 7 0.12317583651199016 1102 8 0.11445008490771613 1102 9 0.20216859396590792 1102 10-11 0.18148512520452442 1102 12-13 0.08167521488617524 1102 14-15 0.27618464396733344 1102 16-17 0.20161879720303943 1102 18-19 0.24588627138554386 1102 20-21 0.18712524105464468 1102 22-23 0.3362722236591509 1102 24-25 0.5424160204869466 1102 26-27 0.36498291451055564 1102 28-29 0.5028419832642186 1102 30-31 0.6267802667069375 1102 32-33 0.4989760314253431 1102 34-35 0.4668475495477722 1102 36-37 0.5684210553662936 1102 38-39 0.6301550188943708 1102 40-41 0.5987799870474504 1102 42-43 0.6495973428125694 1102 44-45 0.5583565531129224 1102 46-47 0.47430945425712423 1102 48-49 0.30904370594313946 1102 50-51 0.19417115609017088 1102 52-53 0.5047653900840885 1102 54-55 0.5979388689018208 1102 56-57 0.5710135012896345 1102 58-59 0.654414121714531 1102 60-61 0.6233236755037268 1102 62-63 0.7212102451950884 1102 64-65 0.46669557798328043 1102 66-67 0.458895223143557 1102 68-69 0.7213277777990754 1102 70-71 0.6052530085398402 1102 72-73 0.42295397282283176 1102 74-75 0.4517463055352948 1102 76-77 0.3528379950169338 1102 78-79 0.40512561275135894 1102 80-81 0.29670214592475475 1102 82-83 0.2778413601773515 1102 84-85 0.3426192566305133 1102 86-87 0.36521826511140176 1102 88-89 0.43681488762474174 1102 90-91 0.5449436705798831 1102 92-93 0.4389140436356129 1102 94-95 0.41775971041591475 1102 96-97 0.36974834611372387 1102 98-99 0.5879441891796695 1102 100-101 0.5735891620851312 1102 102-103 0.7892155088783923 1102 104-105 0.4388199737957912 1102 106-107 0.47443887836463006 1102 108-109 0.3980052649683046 1102 110-111 0.5234913546517532 1102 112-113 0.37005857267775255 1102 114-115 0.4827878991182786 1102 116-117 0.5184867260667758 1102 118-119 0.6669167011303756 1102 120-121 0.5747657557064549 1102 122-123 0.5434319616939511 1102 124-125 0.6012035479488702 1103 1 0.04286067573979224 1103 2 0.2571809970657064 1103 3 0.38836987308755866 1103 4 0.17878922450233858 1103 5 0.2874441207153282 1103 6 0.08024016995967287 1103 7 -0.025180643911973277 1103 8 0.014605414068483924 1103 9 0.30081117699847937 1103 10-11 0.1970965624561103 1103 12-13 0.18142994328857043 1103 14-15 0.19752213083288694 1103 16-17 0.14991331728161583 1103 18-19 0.12070353119640487 1103 20-21 0.030721054020062866 1103 22-23 0.271646441302714 1103 24-25 0.15162184304622173 1103 26-27 0.11743991345980476 1103 28-29 0.26087826861301977 1103 30-31 0.4710699213279881 1103 32-33 0.5066668236040641 1103 34-35 0.338177041530038 1103 36-37 0.28414299332599313 1103 38-39 0.3782076937538861 1103 40-41 0.4469528706876389 1103 42-43 0.6358247287141623 1103 44-45 0.6482104751742312 1103 46-47 0.24935628143059319 1103 48-49 0.2368856014157501 1103 50-51 0.16031996371044244 1103 52-53 0.2075127746151324 1103 54-55 0.24821412131170462 1103 56-57 0.09423149891402005 1103 58-59 0.16618230436334613 1103 60-61 0.21241665599621484 1103 62-63 0.17349649628010866 1103 64-65 0.0968551330109193 1103 66-67 0.07562194661196031 1103 68-69 -0.18975769892439587 1103 70-71 -0.4548613753973001 1103 72-73 -0.31539348480428586 1103 74-75 -0.3842286704800557 1103 76-77 -0.29986713084537087 1103 78-79 -0.3512074769283906 1103 80-81 -0.32171349663543936 1103 82-83 -0.258269814385212 1103 84-85 -0.13216688024188983 1103 86-87 -0.1858676113562332 1103 88-89 -0.06749235573950685 1103 90-91 -0.16006226847626337 1103 92-93 -0.13518424774362003 1103 94-95 -0.18008338428919046 1103 96-97 -0.18956083886503095 1103 98-99 -0.17948322870145716 1103 100-101 -0.18481688661513473 1103 102-103 -0.22162041327428383 1103 104-105 -0.24232694931627563 1103 106-107 -0.328996922722677 1103 108-109 -0.34020085454377735 1103 110-111 -0.4347939142047501 1103 112-113 -0.26836426527738766 1103 114-115 -0.3983239036136865 1103 116-117 -0.4110726416521686 1103 118-119 -0.4236496221370203 1103 120-121 -0.44724872645353386 1103 122-123 -0.35705172874416746 1103 124-125 -0.23547954629938062 1104 1 0.37049601987825653 1104 2 0.5712645495524704 1104 3 0.5028925047823591 1104 4 0.6592334011998631 1104 5 0.7711437395621346 1104 6 0.4787161364552688 1104 7 0.4755839795109935 1104 8 0.4337377981874866 1104 9 0.5667697946520107 1104 10-11 0.7945092969116629 1104 12-13 0.596506058074759 1104 14-15 0.7116618715560321 1104 16-17 0.8205561222910589 1104 18-19 0.608010492745116 1104 20-21 0.7549656987598041 1104 22-23 0.7279876023717975 1104 24-25 0.6862529122446404 1104 26-27 0.6525184611386976 1104 28-29 0.7346088199528467 1104 30-31 0.6081634255044506 1104 32-33 0.73653753273436 1104 34-35 0.8474814079931932 1104 36-37 0.8554064756064221 1104 38-39 1.071091192948714 1104 40-41 0.8261119587004302 1104 42-43 0.7235145812345252 1104 44-45 0.9233865702655848 1104 46-47 0.9101111153567061 1104 48-49 0.9010276591795758 1104 50-51 0.891564967591485 1104 52-53 0.8685992349879399 1104 54-55 0.9073412341631695 1104 56-57 0.8272736574691137 1104 58-59 0.9314718765217904 1104 60-61 0.802808757204545 1104 62-63 0.8601326745532134 1104 64-65 0.8991739114670736 1104 66-67 0.8071985769397116 1104 68-69 0.729283451718004 1104 70-71 0.8036624379882511 1104 72-73 0.523133398660157 1104 74-75 0.5403617925805264 1104 76-77 0.7423218354069299 1104 78-79 0.5962536257512703 1104 80-81 0.5799918453018407 1104 82-83 0.5008767036819144 1104 84-85 0.572986797480926 1104 86-87 0.6540741150707738 1104 88-89 0.6409964124058192 1104 90-91 0.7246403393530159 1104 92-93 0.7903460649410192 1104 94-95 0.7522083427170756 1104 96-97 0.720940568737177 1104 98-99 0.6704941801519553 1104 100-101 0.621853583859977 1104 102-103 0.6547871889361723 1104 104-105 0.684441531979413 1104 106-107 0.5967390273219273 1104 108-109 0.6453900487545283 1104 110-111 0.5547808509052494 1104 112-113 0.36026914413211486 1104 114-115 0.47003343039931167 1104 116-117 0.5119190633422122 1104 118-119 0.6408350680167132 1104 120-121 0.7296587772826975 1104 122-123 0.6282191333108145 1104 124-125 0.9574343640543148 1105 1 0.11602596715242797 1105 2 0.23380121091513573 1105 3 0.23001692332898926 1105 4 0.03082853778249728 1105 5 0.09483654367818417 1105 6 0.04393178011513754 1105 7 -0.03646090674868674 1105 8 0.012010153995369421 1105 9 -0.08694873479073806 1105 10-11 -0.05850358792869059 1105 12-13 -0.17701334957741466 1105 14-15 0.0827330763773162 1105 16-17 -0.05943509007040859 1105 18-19 0.11837200566045425 1105 20-21 -0.07173139660686445 1105 22-23 0.09355197944741889 1105 24-25 0.29915466256482404 1105 26-27 0.2051049930431006 1105 28-29 0.4720524764975593 1105 30-31 0.2502792227400832 1105 32-33 0.37252554606273947 1105 34-35 0.30982277309878725 1105 36-37 0.0902637388530323 1105 38-39 0.2698962681564865 1105 40-41 0.2597742971547987 1105 42-43 0.14815642259131323 1105 44-45 0.36185438865808095 1105 46-47 0.1150536769117565 1105 48-49 0.41188468956762136 1105 50-51 0.28075686522242194 1105 52-53 0.2759631097543931 1105 54-55 0.2419707672817566 1105 56-57 0.2596961626945671 1105 58-59 0.2672178832746894 1105 60-61 0.05401671098766769 1105 62-63 0.04111298137209474 1105 64-65 0.06207233523639388 1105 66-67 0.02105975997208276 1105 68-69 0.04845561181380731 1105 70-71 0.09081169478133688 1105 72-73 0.08655478394681637 1105 74-75 0.04258850256627511 1105 76-77 -0.11806074566388602 1105 78-79 -0.23708617587541525 1105 80-81 -0.15293601844263804 1105 82-83 0.007769594288287607 1105 84-85 -0.0987006508882331 1105 86-87 0.06860254876430005 1105 88-89 1.1767627337633257E-4 1105 90-91 0.015212478380668415 1105 92-93 -0.014731914955525838 1105 94-95 -0.11106279335548663 1105 96-97 -0.11561767085210306 1105 98-99 -0.17235678482500916 1105 100-101 0.05729562702751778 1105 102-103 0.022532535923147634 1105 104-105 0.02306154007402128 1105 106-107 0.14340914990532738 1105 108-109 0.138570619479772 1105 110-111 0.15535980936217442 1105 112-113 0.08063789229558083 1105 114-115 0.061239138760598166 1105 116-117 0.05584270236708733 1105 118-119 0.03511520240937216 1105 120-121 0.10867281532734907 1105 122-123 0.1949642973374921 1105 124-125 -0.06204813551600452 1106 1 -0.23542920544721113 1106 2 -0.2586898289943207 1106 3 0.03688048742395367 1106 4 -0.17584504544529267 1106 5 -0.2555003018309918 1106 6 -0.4304248449623387 1106 7 -0.3252116141242567 1106 8 -0.27499704102471156 1106 9 -0.06853675925919589 1106 10-11 0.0012662792194433337 1106 12-13 -0.15948593035296454 1106 14-15 -0.04655833540958554 1106 16-17 0.08309367653608035 1106 18-19 -0.17589959961050994 1106 20-21 -0.17591501783082464 1106 22-23 -0.3666045863452396 1106 24-25 -4.2165928649891455 1106 26-27 -1.115109779653551 1106 28-29 -0.8288522905400839 1106 30-31 -0.6254712690543514 1106 32-33 -5.705211660544819 1106 34-35 -1.356652340754998 1106 36-37 -0.5204391903555177 1106 38-39 -0.10835968053520872 1106 40-41 -0.807672952838459 1106 42-43 -0.3966756936069231 1106 44-45 -0.4238121612354746 1106 46-47 -0.6068884395559451 1106 48-49 -0.4797918579006577 1106 50-51 -0.2564349211366803 1106 52-53 -0.021007975427096426 1106 54-55 -0.18538715128247674 1106 56-57 -0.19495567378715606 1106 58-59 -0.11549318412136245 1106 60-61 -0.19882693880079216 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-1.623668071900532 2216 68-69 -1.605361024839297 2216 70-71 -1.59227057636582 2216 72-73 -1.3596708785445877 2216 74-75 -1.3860878373314591 2216 76-77 -1.033036020642772 2216 78-79 -0.9592737994488552 2216 80-81 -1.1389090985506023 2216 82-83 -0.932503186970024 2216 84-85 -1.1003443928882533 2216 86-87 -1.1754194247456908 2216 88-89 -1.236107200859319 2216 90-91 -1.0626579043645883 2216 92-93 -1.025059381980796 2216 94-95 -0.9100550541276462 2216 96-97 -1.1467323428683933 2216 98-99 -0.9977095417785335 2216 100-101 -1.2201144252883935 2216 102-103 -1.177012644936699 2216 104-105 -0.9998564670989261 2216 106-107 -0.9480541387084855 2216 108-109 -1.0843376051548645 2216 110-111 -1.3002198887018466 2216 112-113 -1.3369655787143486 2216 114-115 -1.3954462616965628 2216 116-117 -1.1647211576296357 2216 118-119 -1.3224441507306182 2216 120-121 -1.4477338516791818 2216 122-123 -1.419144878545655 2216 124-125 -1.5688159986376249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13218.0 3 2086.0 4 1394.0 5 1219.0 6 1576.0 7 1810.0 8 1672.0 9 1917.0 10 2526.0 11 2937.0 12 3351.0 13 3423.0 14 3276.0 15 3011.0 16 2642.0 17 2402.0 18 2162.0 19 1943.0 20 1857.0 21 1744.0 22 2185.0 23 3151.0 24 5170.0 25 7802.0 26 8589.0 27 7417.0 28 8238.0 29 10346.0 30 11953.0 31 15104.0 32 19485.0 33 25485.0 34 35567.0 35 52314.0 36 87204.0 37 132750.0 38 49748.0 39 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.332801276935356 18.52595630950834 9.256482117336997 20.884760296219305 2 19.242490608620766 21.50811444861839 37.87472716744124 21.374667775319605 3 17.210876904488366 31.177465186073317 29.973848954978997 21.63780895445932 4 10.280833498827926 16.510359342896756 36.958995079706526 36.24981207856879 5 10.306727742471937 45.37421304270357 33.57876254677199 10.740296668052503 6 28.921447622158663 37.929645667026115 19.803187530508346 13.345719180306876 7 28.242567550010577 36.47329366390367 20.783576410671397 14.500562375414358 8 27.944779354991983 34.215010096810595 20.04105541367227 17.79915513452515 9 29.01706831781668 14.433085363861792 18.602907330690304 37.94693898763122 10-11 23.431592545366467 29.1631633749645 28.590665189251997 18.814578890417035 12-13 24.041991632855332 28.393166708118805 28.030677055685686 19.534164603340177 14-15 22.55439044558264 29.125326092101123 25.3719375647892 22.948345897527037 16-17 21.729660402713215 30.98360062401338 26.369483852586583 20.91725512068682 18-19 21.199677042151567 29.66339996659057 28.156912968428088 20.980010022829777 20-21 23.010667866937037 28.49197473863958 27.737474884806524 20.759882509616855 22-23 22.77678282807641 28.907117936218356 27.140731300415773 21.17536793528946 24-25 21.92951928600529 29.069837584207775 27.79564249408144 21.20500063570549 26-27 21.932285465308897 29.012215640437176 27.56393421019662 21.491564684057305 28-29 21.797733205760807 28.371028168347472 27.937547619290676 21.893691006601042 30-31 22.008591624975292 28.657495956145407 28.077482388468034 21.256430030411273 32-33 21.320309962414736 29.02157672497796 27.70395805299058 21.954155259616723 34-35 21.80182002772916 28.819116416071033 28.10083094678191 21.27823260941789 36-37 21.845048516299546 28.771334922084378 27.542744084871973 21.8408724767441 38-39 21.64456125111826 28.817313867204174 27.74201810750916 21.796106774168404 40-41 21.52419717065529 28.70687959137462 28.27368026400288 21.49524297396721 42-43 21.65760140762855 28.614356363514876 27.81848199213591 21.909560236720658 44-45 21.67710227077632 28.900266435032446 27.694951840313447 21.727679453877784 46-47 21.838029763650336 28.80091614728144 27.461124990255726 21.8999290988125 48-49 21.499769390457686 29.022651703494596 27.472417335527137 22.005161570520585 50-51 21.698838115742973 28.592282564311965 27.88290211217937 21.825977207765693 52-53 21.79300027747952 28.602941654130593 27.78767155704988 21.816386511340003 54-55 21.447105399430935 29.038529712553757 27.69057442847624 21.82379045953907 56-57 21.70530190050271 29.13618490715572 27.581093157948956 21.57742003439261 58-59 21.86326642352701 28.550349397254937 27.693235706265025 21.893148472953033 60-61 21.617031701973055 29.38889182760794 27.73627997145421 21.257796498964797 62-63 21.5883707312003 28.95995293056423 27.702293242956056 21.749383095279416 64-65 21.36166137387408 29.01548489837281 27.71403329355232 21.90882043420078 66-67 21.31530153733651 29.48649656915787 27.300668721892983 21.897533171612636 68-69 21.64649961998287 29.19997809913482 27.53903875617934 21.61448352470297 70-71 21.367852913772815 28.767358468854056 27.975300026355548 21.889488591017585 72-73 21.652107822985002 29.180544200389573 27.303933532606827 21.863414444018595 74-75 21.370869328374066 29.499368491776472 27.385648755218877 21.744113424630584 76-77 21.06277126781424 29.35209390680405 27.902269810380492 21.68286501500122 78-79 21.368197940195515 29.17997583469596 27.41499919263385 22.03682703247468 80-81 21.185214606473597 29.619600269864705 27.13736466164367 22.057820462018025 82-83 21.804445690030374 29.306141615077447 27.080141390500778 21.8092713043914 84-85 21.238111058420884 29.176198081436404 27.384493173109668 22.20119768703304 86-87 21.16880308765652 29.07670049861681 27.688220189479956 22.066276224246714 88-89 21.596096434692953 28.83760726591739 27.7014492672927 21.86484703209695 90-91 21.393751374606644 29.22351080800422 27.528974620005364 21.85376319738377 92-93 21.285380203789973 29.324412108613746 27.413277900480708 21.976929787115573 94-95 21.107481579093896 28.930845041667443 27.839789157185546 22.12188422205312 96-97 21.432840253159856 29.035060041945844 27.556747526865756 21.975352178028544 98-99 21.026466712448265 29.124519757233802 27.35945359045268 22.489559939865252 100-101 20.93693362621209 29.27330617987173 27.568929981003716 22.220830212912464 102-103 21.27158446466073 29.122311425068837 27.415320967845215 22.190783142425218 104-105 20.749774030279013 29.25449480223241 27.682636430959057 22.31309473652951 106-107 20.726369017955992 29.412228708985605 27.64558329366107 22.215818979397334 108-109 20.98911164255229 29.858116341073877 26.728676386657796 22.42409562971604 110-111 20.857469519268427 30.029900720698315 26.843274565084645 22.269355194948616 112-113 20.941129087639133 29.756639426565872 27.304566351577336 21.997665134217662 114-115 20.73226977832757 29.941879079159712 27.103391763589002 22.22245937892372 116-117 20.899763542482543 30.004213175546507 26.980842115422448 22.1151811665485 118-119 20.835664987875397 29.964985740946453 26.81982736940183 22.379521901776325 120-121 20.697306451987103 29.774261652321183 26.992459921583183 22.53597197410853 122-123 20.858072455079817 29.742997722670655 26.951366861610015 22.44756296063951 124-125 21.130198101117227 29.76166060834406 26.626211857855697 22.481929432683017 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 100.0 2 145.0 3 158.0 4 151.0 5 137.5 6 141.5 7 142.5 8 137.0 9 142.5 10 141.5 11 147.5 12 173.0 13 186.0 14 210.0 15 242.0 16 272.0 17 325.5 18 405.0 19 525.0 20 671.0 21 924.0 22 1256.5 23 1819.0 24 2371.5 25 2788.5 26 3667.5 27 4483.0 28 5057.5 29 6145.5 30 7769.0 31 9238.0 32 10664.5 33 12987.0 34 15342.5 35 16284.5 36 18313.0 37 21059.5 38 20879.0 39 19820.0 40 19671.0 41 19546.5 42 19004.0 43 18918.5 44 19717.0 45 20552.5 46 20110.0 47 18691.0 48 17684.5 49 17046.0 50 16906.5 51 15548.5 52 14235.5 53 13822.5 54 13156.0 55 12573.5 56 11562.5 57 10344.5 58 9560.0 59 8901.0 60 7800.5 61 6583.5 62 5232.0 63 3752.5 64 2786.0 65 2314.5 66 1698.0 67 1252.0 68 974.0 69 634.5 70 470.5 71 445.5 72 397.0 73 365.5 74 321.5 75 280.5 76 205.5 77 94.0 78 58.5 79 42.5 80 23.5 81 17.0 82 8.0 83 5.5 84 5.5 85 2.5 86 2.5 87 2.5 88 2.5 89 2.0 90 0.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.423987646484556E-4 2 3.711993823242278E-4 3 1.855996911621139E-4 4 9.279984558105695E-4 5 0.001855996911621139 6 0.0012991978381347974 7 0.0014847975292969111 8 0.001855996911621139 9 0.0033407944409180506 10-11 0.002783995367431709 12-13 0.003711993823242278 14-15 0.005103991506958132 16-17 0.004361592742309677 18-19 0.004454392587890734 20-21 0.004918391815796019 22-23 0.09651183940429924 24-25 0.004175993051147563 26-27 0.0032479945953369934 28-29 0.0028767952130127655 30-31 0.0025055958306885377 32-33 0.0035263941320801645 34-35 0.002041596602783253 36-37 0.0011135981469726835 38-39 0.002227196293945367 40-41 0.002227196293945367 42-43 0.0028767952130127655 44-45 0.0025055958306885377 46-47 0.0029695950585938223 48-49 8.351986102295126E-4 50-51 0.002598395676269595 52-53 0.0028767952130127655 54-55 0.002783995367431709 56-57 0.0028767952130127655 58-59 0.0016703972204590253 60-61 0.001948796757202196 62-63 0.0028767952130127655 64-65 0.002041596602783253 66-67 9.279984558105695E-4 68-69 4.6399922790528475E-4 70-71 0.0014847975292969111 72-73 6.495989190673987E-4 74-75 8.351986102295126E-4 76-77 8.351986102295126E-4 78-79 9.279984558105695E-4 80-81 0.0010207983013916266 82-83 4.6399922790528475E-4 84-85 0.0010207983013916266 86-87 6.495989190673987E-4 88-89 8.351986102295126E-4 90-91 6.495989190673987E-4 92-93 7.423987646484556E-4 94-95 7.423987646484556E-4 96-97 7.423987646484556E-4 98-99 7.423987646484556E-4 100-101 8.351986102295126E-4 102-103 0.0017631970660400822 104-105 9.279984558105695E-4 106-107 8.351986102295126E-4 108-109 0.0013919976837158544 110-111 0.002041596602783253 112-113 9.279984558105695E-4 114-115 6.495989190673987E-4 116-117 0.0014847975292969111 118-119 0.0025055958306885377 120-121 0.0012063979925537403 122-123 9.279984558105695E-4 124-125 6.495989190673987E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 538794.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.428022379265066 #Duplication Level Percentage of deduplicated Percentage of total 1 55.90005306777047 19.245282780193065 2 13.997586884229893 9.638184690119479 3 9.457056291496107 9.767632369367925 4 6.35885567927395 8.756913025302412 5 4.101498237031607 7.06032365465202 6 2.6784231982197553 5.532768828567146 7 1.7213609823734026 4.148413809676164 8 1.1865118675635717 3.2679405703793787 9 0.8303233678642994 2.572775234177296 >10 3.4866225758394638 20.790107607030695 >50 0.2039953384521565 4.859726729161242 >100 0.07663316947022351 4.118765183160393 >500 0.0010793404150735706 0.2411655182128379 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 797 0.1479229538562048 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.711993823242278E-4 0.0 0.0 0.0 0.0 8 3.711993823242278E-4 0.0 0.0 0.0 0.0 9 3.711993823242278E-4 0.0 0.0 0.0 0.0 10-11 3.711993823242278E-4 0.0 0.0 0.0 0.0 12-13 3.711993823242278E-4 0.0 0.0 0.0 0.0 14-15 3.711993823242278E-4 0.0 0.0 0.0 0.0 16-17 3.711993823242278E-4 0.0 0.0 0.0 0.0 18-19 7.423987646484557E-4 0.0 0.0 0.0 0.0 20-21 0.0011135981469726835 0.0 0.0 0.0 0.0 22-23 0.0011135981469726835 0.0 0.0 9.279984558105695E-5 0.0 24-25 0.0011135981469726835 0.0 0.0 3.711993823242278E-4 0.0 26-27 0.0011135981469726835 0.0 0.0 5.567990734863418E-4 0.0 28-29 0.0011135981469726835 0.0 0.0 8.351986102295126E-4 0.0 30-31 0.0011135981469726835 0.0 0.0 0.0029695950585938227 0.0 32-33 0.0011135981469726835 0.0 0.0 0.007980786719970897 0.0 34-35 0.0011135981469726835 0.0 0.0 0.01698237174133342 0.0 36-37 0.0011135981469726835 0.0 0.0 0.03006714996826245 0.0 38-39 0.0011135981469726835 0.0 0.0 0.05614390657653946 0.0 40-41 0.0011135981469726835 0.0 0.0 0.08454065932434288 0.0 42-43 0.0011135981469726835 0.0 0.0 0.1181342034246855 0.0 44-45 0.0011135981469726835 0.0 0.0 0.1647197259063761 0.0 46-47 0.0011135981469726835 0.0 0.0 0.22782362090149483 0.0 48-49 0.0011135981469726835 0.0 0.0 0.29241231342591045 0.0 50-51 0.0011135981469726835 0.0 0.0 0.3533818119726648 0.0 52-53 0.0011135981469726835 0.0 0.0 0.426879289672862 0.0 54-55 0.0011135981469726835 0.0 0.0 0.5160599412762578 0.0 56-57 0.0014847975292969111 0.0 0.0 0.6206453672461089 0.0 58-59 0.0014847975292969111 0.0 0.0 0.7402643682000913 0.0 60-61 0.0014847975292969111 0.0 0.0 0.8719473490796111 0.0 62-63 0.0014847975292969111 0.0 0.0 1.0129103145172367 0.0 64-65 0.0014847975292969111 0.0 0.0 1.1720620496887493 0.0 66-67 0.0014847975292969111 0.0 0.0 1.3610025352917812 0.0 68-69 0.0014847975292969111 0.0 0.0 1.582237367157021 0.0 70-71 0.0014847975292969111 0.0 0.0 1.8172065761682572 0.0 72-73 0.0014847975292969111 0.0 0.0 2.061084570355275 0.0 74-75 0.0014847975292969111 0.0 0.0 2.3251929308789627 0.0 76-77 0.0014847975292969111 0.0 0.0 2.6408052057001377 0.0 78-79 0.0014847975292969111 0.0 0.0 3.0026318036206785 0.0 80-81 0.0014847975292969111 0.0 0.0 3.3673351967542327 0.0 82-83 0.0014847975292969111 0.0 0.0 3.7449377684235534 0.0 84-85 0.0014847975292969111 0.0 0.0 4.143327505503031 0.0 86-87 0.0014847975292969111 0.0 0.0 4.605841935879019 0.0 88-89 0.0014847975292969111 0.0 0.0 5.096381919620486 0.0 90-91 0.0014847975292969111 0.0 0.0 5.626733037116226 0.0 92-93 0.001670397220459025 0.0 0.0 6.162373745810087 0.0 94-95 0.001670397220459025 0.0 0.0 6.768635136991132 0.0 96-97 0.001670397220459025 0.0 0.0 7.405520477213926 0.0 98-99 0.001670397220459025 0.0 0.0 8.083330549337965 0.0 100-101 0.0018559969116211392 0.0 0.0 8.808468542708344 0.0 102-103 0.002041596602783253 0.0 0.0 9.564323284966054 0.0 104-105 0.002227196293945367 0.0 0.0 10.331221208847907 0.0 106-107 0.002227196293945367 0.0 0.0 11.138579865403104 0.0 108-109 0.002227196293945367 0.0 0.0 11.95558970589873 0.0 110-111 0.002227196293945367 0.0 0.0 12.83895143598481 0.0 112-113 0.002227196293945367 0.0 0.0 13.725189961283904 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1105 0.0 71.069725 1 GTACATG 6410 0.0 55.41014 1 TACATGG 6480 0.0 53.89345 2 GTATCAA 1525 0.0 51.496418 2 ACATGGG 6505 0.0 51.034027 3 CATGGGA 4870 0.0 49.354336 4 ATGGGAT 1895 0.0 49.29052 5 CATGGGG 1475 0.0 44.368385 4 ATGGGAG 1580 0.0 42.92602 5 TCAACGC 1830 0.0 41.93837 5 ATCAACG 1895 0.0 40.81381 4 CAACGCA 1890 0.0 40.29221 6 AACGCAG 1945 0.0 39.458725 7 GTCGGGA 280 0.0 38.24612 2 ATGGGGG 700 0.0 35.69638 5 GGATTCG 100 1.0618791E-5 35.69638 8 TGGGATA 605 0.0 35.401367 6 AGTACAT 3595 0.0 33.594646 2 TGGGATC 700 0.0 33.14664 6 GAGTACA 3740 0.0 32.769405 1 >>END_MODULE