FastQCFastQC Report
Thu 26 May 2016
SRR936218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538794
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.5738742450732562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.40238013043946297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.35134021536988164No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6550.12156779771118462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.080.842861
GATAGCG350.0077968350.9953467
GTATCAA33150.050.8232542
GTACATG58000.049.189091
ACATGGG58900.047.808533
TACATGG59750.047.6265952
ATTCACG909.668656E-846.3036353
CATATAG1700.045.525423
CATGGGA43300.044.9592864
CATGGGG12550.044.116274
AACGCGA355.454681E-542.4961276-77
ACGTTTA1002.2242057E-741.64627
ATGGGAT18450.040.9795275
ATGGGAG13900.038.9750025
TCAACGC46400.037.8498045
ATCAACG46600.037.6873554
ATGGGAC7900.037.6792685
CAACGCA47350.037.3176086
ACGCGAA401.198064E-437.18410578-79
AACGCAG49050.036.0242357
Produced by FastQC (version 0.11.5)