FastQCFastQC Report
Thu 26 May 2016
SRR936217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041219
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG160400.054.1889761
GGTATCA28600.053.252381
TACATGG166050.051.915212
ACATGGG169350.049.9550633
CATGGGA112800.046.3601574
CATGGGG51550.041.8931544
GTATCAA40550.040.4933171
ATGGGAT46000.040.4810335
ATGGGAG43950.037.9021845
TCAACGC47800.032.8584
ATGGGGG28700.032.3376055
ATCAACG48950.031.964523
CAACGCA49350.031.8259875
GAGTACA99200.030.2862031
AGTACAT94350.030.0144332
AACGCAG53050.029.8305556
GTCGGGA4500.027.7633512
TATCAAC58700.026.7566013
TACCTGG19250.026.5786462
TGGGATA16650.026.0839716
Produced by FastQC (version 0.11.5)