##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2041219 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.16994844747183 31.0 30.0 34.0 26.0 34.0 2 30.17147988530383 31.0 30.0 34.0 26.0 34.0 3 30.292393417854722 31.0 30.0 34.0 26.0 34.0 4 33.94049487095701 37.0 35.0 37.0 30.0 37.0 5 34.031550754720584 35.0 35.0 37.0 30.0 37.0 6 34.17301132313583 37.0 35.0 37.0 30.0 37.0 7 33.993986436536204 37.0 35.0 37.0 30.0 37.0 8 33.98077521324267 37.0 35.0 37.0 30.0 37.0 9 35.51188676962148 39.0 35.0 39.0 30.0 39.0 10-11 35.31641705275132 39.0 35.0 39.0 29.0 39.0 12-13 35.346251186178456 39.0 35.0 39.0 29.5 39.0 14-15 36.47120054242097 40.0 36.0 41.0 29.5 41.0 16-17 36.463224916091804 40.0 36.0 41.0 29.5 41.0 18-19 36.472008148072305 40.0 36.0 41.0 29.0 41.0 20-21 36.56877899921567 40.0 36.0 41.0 29.0 41.0 22-23 36.41489227760471 39.5 36.0 41.0 28.5 41.0 24-25 36.36518080617513 39.0 36.0 41.0 28.5 41.0 26-27 36.46234357998823 39.0 36.0 41.0 29.5 41.0 28-29 36.344946818543235 39.0 36.0 41.0 29.0 41.0 30-31 36.27660677271767 39.0 36.0 41.0 29.0 41.0 32-33 36.125943370113646 39.0 35.5 41.0 28.5 41.0 34-35 36.095177930442546 39.0 35.0 41.0 29.0 41.0 36-37 35.98019541264313 39.0 35.0 41.0 28.0 41.0 38-39 35.72947366255164 39.0 35.0 41.0 27.0 41.0 40-41 35.840110982702 39.0 35.0 40.5 27.5 41.0 42-43 35.75051746039989 39.0 35.0 41.0 27.0 41.0 44-45 35.598093590153724 39.0 35.0 40.5 27.0 41.0 46-47 35.5707951963998 39.0 35.0 40.0 27.0 41.0 48-49 35.48173003484683 39.0 35.0 40.0 27.0 41.0 50-51 35.345093789544386 39.0 34.0 40.0 27.0 41.0 52-53 35.200703354221176 38.5 34.0 40.0 27.0 41.0 54-55 35.02363955068026 38.0 34.0 40.0 26.0 41.0 56-57 34.82602405719328 38.0 34.0 40.0 26.0 41.0 58-59 34.60063226924696 38.0 33.5 40.0 26.0 41.0 60-61 34.35840176874701 38.0 33.0 40.0 26.0 41.0 62-63 34.110539339482926 37.0 33.0 40.0 25.5 41.0 64-65 33.82062777193432 37.0 33.0 40.0 24.5 41.0 66-67 33.52094606213248 36.0 32.0 39.0 24.0 41.0 68-69 33.230457633404356 36.0 32.0 39.0 23.0 41.0 70-71 32.885547802563075 35.5 32.0 39.0 23.0 40.0 72-73 32.55029029222244 35.0 32.0 38.5 22.5 40.0 74-75 32.19612324792195 35.0 31.0 37.0 22.0 39.5 76-77 31.867034355451324 35.0 31.0 37.0 21.5 39.0 78-79 31.524469691885095 35.0 31.0 36.5 20.5 39.0 80-81 31.210654270805826 35.0 31.0 36.0 20.0 38.0 82-83 30.90972502215588 34.0 31.0 35.5 20.0 37.0 84-85 30.953813383081382 34.5 31.0 35.0 20.0 37.0 86-87 30.844149990765324 35.0 31.0 35.0 20.0 36.5 88-89 30.68412429043625 34.0 31.0 35.0 20.0 36.0 90-91 30.525637376489243 34.0 31.0 35.0 19.0 36.0 92-93 30.406147503036177 34.0 31.0 35.0 18.5 36.0 94-95 30.28948069756356 34.0 31.0 35.0 18.0 35.0 96-97 30.19475323323955 34.0 31.0 35.0 18.0 35.0 98-99 30.092356577123766 34.0 31.0 35.0 16.5 35.0 100-101 29.994487362698464 34.0 31.0 35.0 14.0 35.0 102-103 28.98264737884568 33.5 29.0 34.5 10.0 35.0 104-105 29.68506784426365 34.0 30.0 35.0 10.0 35.0 106-107 29.82333252825885 34.0 31.0 35.0 7.0 35.0 108-109 29.779734315622186 34.0 31.0 35.0 7.0 35.0 110-111 29.66771865243269 34.0 31.0 35.0 2.0 35.0 112-113 29.628309603232186 34.0 31.0 35.0 2.0 35.0 114-115 29.517347967072617 34.0 30.5 35.0 2.0 35.0 116-117 29.39148151178291 34.0 30.0 35.0 2.0 35.0 118-119 29.176283877428148 34.0 30.0 35.0 2.0 35.0 120-121 28.96839609076733 34.0 30.0 35.0 2.0 35.0 122-123 28.663609588192152 34.0 30.0 35.0 2.0 35.0 124-125 26.570755269277818 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37725893218588524 1101 2 0.4395690857799508 1101 3 0.42512526308263077 1101 4 0.37839784518825326 1101 5 0.3723601226211528 1101 6 0.33637008379540134 1101 7 0.3599488359080638 1101 8 0.44351700778546643 1101 9 0.4580883232929267 1101 10-11 0.5873586193329459 1101 12-13 0.5328780407977831 1101 14-15 0.5270890807876754 1101 16-17 0.5573015208957912 1101 18-19 0.606495640652561 1101 20-21 0.48885081479128445 1101 22-23 0.5882824814538736 1101 24-25 0.6180878100642744 1101 26-27 0.5161792464046897 1101 28-29 0.5801608367339455 1101 30-31 0.591242097514062 1101 32-33 0.6971572822072076 1101 34-35 0.47255415058073424 1101 36-37 0.4774606569233697 1101 38-39 0.7066758571932255 1101 40-41 0.5922246238749622 1101 42-43 0.7145367147671848 1101 44-45 0.6047506526388986 1101 46-47 0.4573050864194741 1101 48-49 0.5075617681214837 1101 50-51 0.48800561101955964 1101 52-53 0.47663301505185274 1101 54-55 0.47255422920426327 1101 56-57 0.5482156283082844 1101 58-59 0.39261632702933724 1101 60-61 0.31721417871397506 1101 62-63 0.41497822490738656 1101 64-65 0.43886914570300206 1101 66-67 0.4911295461400016 1101 68-69 0.3270508113886166 1101 70-71 0.3391846719397975 1101 72-73 0.3410415297125837 1101 74-75 0.30051367232065473 1101 76-77 0.3775263951607286 1101 78-79 0.32454358820624307 1101 80-81 0.22039290517199106 1101 82-83 0.2439260446506779 1101 84-85 0.094090253650986 1101 86-87 0.29203598453073454 1101 88-89 0.21544930658182082 1101 90-91 0.2291786427586615 1101 92-93 0.267314191477773 1101 94-95 0.37078896030937614 1101 96-97 0.3400189065764607 1101 98-99 0.3682400011061695 1101 100-101 0.3726273438819234 1101 102-103 0.5052792558182695 1101 104-105 0.3816685005616449 1101 106-107 0.3386112604810414 1101 108-109 0.39246402144188863 1101 110-111 0.32382997469192176 1101 112-113 0.2530381164962634 1101 114-115 0.20211420194577912 1101 116-117 0.06650966433954153 1101 118-119 0.07026286436829565 1101 120-121 0.17212155909091464 1101 122-123 0.2848208408447874 1101 124-125 -0.09345076672311237 1102 1 0.25649960123817905 1102 2 0.2992537095327883 1102 3 0.3573427014917492 1102 4 0.3558819791045664 1102 5 0.29526694482630944 1102 6 0.3553295121986082 1102 7 0.42490660308944683 1102 8 0.33780444220001016 1102 9 0.5026128099334102 1102 10-11 0.5489109011478206 1102 12-13 0.47512702768801063 1102 14-15 0.5295574559725651 1102 16-17 0.4659592317499417 1102 18-19 0.4933752354144758 1102 20-21 0.4663787135376012 1102 22-23 0.6348885358504077 1102 24-25 0.6296910151425834 1102 26-27 0.5346768419561982 1102 28-29 0.49526356098071034 1102 30-31 0.4684505372343821 1102 32-33 0.5542383105362845 1102 34-35 0.4748166905131157 1102 36-37 0.4701268249068491 1102 38-39 0.491819476111381 1102 40-41 0.4054116602185758 1102 42-43 0.5275527144717316 1102 44-45 0.4952031150673122 1102 46-47 0.4176359272092185 1102 48-49 0.3756308221410407 1102 50-51 0.2951615339132019 1102 52-53 0.34187068328109405 1102 54-55 0.4647787746728085 1102 56-57 0.414353989340114 1102 58-59 0.4421690679144987 1102 60-61 0.4787955304569067 1102 62-63 0.5278600318530096 1102 64-65 0.4962003460138007 1102 66-67 0.5266248588443148 1102 68-69 0.4195363173783875 1102 70-71 0.4032149446009825 1102 72-73 0.45135575149878093 1102 74-75 0.3981315375992196 1102 76-77 0.3939025354090262 1102 78-79 0.3818397960394613 1102 80-81 0.4756717045868726 1102 82-83 0.44391886198422625 1102 84-85 0.4182837812445861 1102 86-87 0.38557121638206837 1102 88-89 0.4151643650662713 1102 90-91 0.41513816799701075 1102 92-93 0.463206588349081 1102 94-95 0.4115683794594531 1102 96-97 0.37730683253354513 1102 98-99 0.33001992788949863 1102 100-101 0.34954757547590276 1102 102-103 0.3176947613437342 1102 104-105 0.30267802287749035 1102 106-107 0.32119760781210616 1102 108-109 0.25707691400829447 1102 110-111 0.3787406357948164 1102 112-113 0.3081649361172154 1102 114-115 0.2244344591104408 1102 116-117 0.3664817091303014 1102 118-119 0.47191136871128947 1102 120-121 0.5628509564458106 1102 122-123 0.5243776460916791 1102 124-125 0.5769649254935239 1103 1 0.22702434960860884 1103 2 0.28427673071835 1103 3 0.29917739282903 1103 4 0.3617074030050489 1103 5 0.362270898140018 1103 6 0.33077121334848414 1103 7 0.3436806058898725 1103 8 0.4070516974486793 1103 9 0.4655526952599729 1103 10-11 0.4915559386060835 1103 12-13 0.49076767593766846 1103 14-15 0.4987800656461019 1103 16-17 0.3998224952191194 1103 18-19 0.43759421344137905 1103 20-21 0.31203441280981536 1103 22-23 0.5389698158479774 1103 24-25 0.5603640728586683 1103 26-27 0.4236701261585978 1103 28-29 0.5042809906466843 1103 30-31 0.3309163090256959 1103 32-33 0.5274549531200776 1103 34-35 0.44185583650160964 1103 36-37 0.37059509083425723 1103 38-39 0.5886136345749335 1103 40-41 0.48944893623744434 1103 42-43 0.5731642501965766 1103 44-45 0.5800499101744236 1103 46-47 0.5275653112634728 1103 48-49 0.4164534005624745 1103 50-51 0.4003962910433714 1103 52-53 0.5738951027357544 1103 54-55 0.5585550420834338 1103 56-57 0.42459406526366195 1103 58-59 0.4668351729124325 1103 60-61 0.47277589981361956 1103 62-63 0.35204766436675783 1103 64-65 0.30586669474315187 1103 66-67 0.367415319206728 1103 68-69 0.31536648650073573 1103 70-71 0.43355308325390496 1103 72-73 0.2844068396452002 1103 74-75 0.31926632963016743 1103 76-77 0.32751569445315454 1103 78-79 0.35013666420275413 1103 80-81 0.3867914708927245 1103 82-83 0.333098131251198 1103 84-85 0.3300703305206092 1103 86-87 0.3399262101564595 1103 88-89 0.31688023417987665 1103 90-91 0.32200144012570675 1103 92-93 0.43200478428908795 1103 94-95 0.4481861775733691 1103 96-97 0.45961609665247494 1103 98-99 0.36107831474562957 1103 100-101 0.3452932218540319 1103 102-103 0.31815488343127996 1103 104-105 0.4119004885500388 1103 106-107 0.40265191469203643 1103 108-109 0.2893953581904434 1103 110-111 0.17933025174709272 1103 112-113 0.31667223254043364 1103 114-115 0.38859140579125295 1103 116-117 0.3005851039828684 1103 118-119 0.2528647953851575 1103 120-121 0.15542914533561003 1103 122-123 0.3422973921984749 1103 124-125 0.3636153427509896 1104 1 0.1833641188094468 1104 2 0.255797989966851 1104 3 0.25640342907954405 1104 4 0.24472251241768106 1104 5 0.11250259310682509 1104 6 0.14939473923995905 1104 7 0.13148508749248577 1104 8 0.05171190651645219 1104 9 0.2374507827108232 1104 10-11 0.25419340562673653 1104 12-13 0.2612033195078638 1104 14-15 0.4173661171813592 1104 16-17 0.36638265513068546 1104 18-19 0.3563523952851284 1104 20-21 0.2735959105293446 1104 22-23 0.3123660417250278 1104 24-25 0.3700423293050079 1104 26-27 0.23298376724709158 1104 28-29 0.3122587904093379 1104 30-31 0.3277380194906243 1104 32-33 0.4826597734373763 1104 34-35 0.4600168869478338 1104 36-37 0.44187154253289407 1104 38-39 0.46114492163076903 1104 40-41 0.4331069643979717 1104 42-43 0.478769926160318 1104 44-45 0.42292792893245235 1104 46-47 0.4128417635521018 1104 48-49 0.4319409492135051 1104 50-51 0.3865176519082425 1104 52-53 0.4722678641198783 1104 54-55 0.46601122948707996 1104 56-57 0.38550706290634196 1104 58-59 0.2514906187741772 1104 60-61 0.41807263211071444 1104 62-63 0.47334151592720275 1104 64-65 0.4925973506840151 1104 66-67 0.4920230602425022 1104 68-69 0.4461285375152215 1104 70-71 0.5114265851277651 1104 72-73 0.507091686995949 1104 74-75 0.4189642928089299 1104 76-77 0.3934655464682919 1104 78-79 0.4260106323673547 1104 80-81 0.3985254381664305 1104 82-83 0.44935939605152697 1104 84-85 0.3927917928051947 1104 86-87 0.39920634458821525 1104 88-89 0.34805347062595615 1104 90-91 0.3395081889022826 1104 92-93 0.330031416746678 1104 94-95 0.4503876362117971 1104 96-97 0.3994229980109445 1104 98-99 0.4472265596015603 1104 100-101 0.34525320069690224 1104 102-103 0.3631761777351663 1104 104-105 0.370184184668112 1104 106-107 0.450555568132021 1104 108-109 0.48419170310831205 1104 110-111 0.4250834120664386 1104 112-113 0.3594165983334179 1104 114-115 0.361578403045133 1104 116-117 0.3762156510799528 1104 118-119 0.35144624694564186 1104 120-121 0.3364573272118072 1104 122-123 0.3020724909951724 1104 124-125 0.2613448648246255 1105 1 0.31256739376904363 1105 2 0.3485550350540798 1105 3 0.41842414033904873 1105 4 0.4049893511239304 1105 5 0.28401895792828213 1105 6 0.2193002283223251 1105 7 0.22367613123620345 1105 8 0.3475038095712577 1105 9 0.2915940322635393 1105 10-11 0.4330175727534282 1105 12-13 0.46245038474766176 1105 14-15 0.5466797989835115 1105 16-17 0.41426596973033014 1105 18-19 0.45140004980482473 1105 20-21 0.4150117785200109 1105 22-23 0.25790587132603093 1105 24-25 0.42159268081112344 1105 26-27 0.22490677510194246 1105 28-29 0.34428143046307724 1105 30-31 0.436986587859721 1105 32-33 0.5001559324764386 1105 34-35 0.5392690468648311 1105 36-37 0.4754428713391121 1105 38-39 0.5385188350212573 1105 40-41 0.43340160434529906 1105 42-43 0.6496401449121905 1105 44-45 0.5813677339247008 1105 46-47 0.4754061952561557 1105 48-49 0.53844364619993 1105 50-51 0.5621948914711368 1105 52-53 0.546505824120672 1105 54-55 0.49241841790366436 1105 56-57 0.48311436039958267 1105 58-59 0.4743734345477222 1105 60-61 0.49646847389637827 1105 62-63 0.5573305284510681 1105 64-65 0.6170521337611703 1105 66-67 0.5506271452956781 1105 68-69 0.5457261511311771 1105 70-71 0.5816689481002371 1105 72-73 0.633167322334117 1105 74-75 0.6464266646937702 1105 76-77 0.5690516671309993 1105 78-79 0.5437006684823622 1105 80-81 0.6318766183889721 1105 82-83 0.6517318129637246 1105 84-85 0.6317451226356567 1105 86-87 0.6028627398713127 1105 88-89 0.6503038414200226 1105 90-91 0.5986496759361302 1105 92-93 0.5525956489202954 1105 94-95 0.5317684533554541 1105 96-97 0.6256509415837357 1105 98-99 0.6637423590867719 1105 100-101 0.6276456371630843 1105 102-103 0.7063169863655219 1105 104-105 0.577979506344434 1105 106-107 0.5162090635727488 1105 108-109 0.5885600895056591 1105 110-111 0.6180193694467384 1105 112-113 0.4925393162040308 1105 114-115 0.574852498008319 1105 116-117 0.5478430878454326 1105 118-119 0.6483235955851754 1105 120-121 0.6273002102936225 1105 122-123 0.776561526797586 1105 124-125 0.787823219097568 1106 1 -0.16724873209594904 1106 2 -0.1810987798409478 1106 3 -0.010292452023175969 1106 4 0.06759393717223361 1106 5 0.07373158999678964 1106 6 0.006621181235701101 1106 7 -0.18431868232932658 1106 8 -0.08093889770994878 1106 9 -0.0828721736999185 1106 10-11 -0.031072710668297532 1106 12-13 0.0671961601994795 1106 14-15 -0.19733771462853156 1106 16-17 -0.038176681176601335 1106 18-19 -0.13566674902823195 1106 20-21 -0.18883153854307722 1106 22-23 -0.2561879354886756 1106 24-25 -4.2140563046865935 1106 26-27 -1.1064429662810156 1106 28-29 -0.6898090408729516 1106 30-31 -0.6426752133927209 1106 32-33 -5.851444392615285 1106 34-35 -1.3780909452427679 1106 36-37 -0.5521135851610808 1106 38-39 -0.15020020022677016 1106 40-41 -0.591653478031624 1106 42-43 -0.18544744663730484 1106 44-45 -0.23978718731572002 1106 46-47 -0.23367170931156522 1106 48-49 -0.029535758393635092 1106 50-51 -0.03707318236038759 1106 52-53 -0.023383259468303663 1106 54-55 -0.2791059898139707 1106 56-57 -0.013616165807420089 1106 58-59 0.09484622690712996 1106 60-61 0.08353691697949017 1106 62-63 -0.03776584809158834 1106 64-65 -0.007863187360861446 1106 66-67 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-0.8387166448284482 2216 82-83 -0.8150374109371938 2216 84-85 -0.9754836742452149 2216 86-87 -0.7695259570069517 2216 88-89 -0.7001735705661538 2216 90-91 -0.6734489278221538 2216 92-93 -0.6196696258450416 2216 94-95 -0.7492009171266645 2216 96-97 -0.8278171546836504 2216 98-99 -0.7195160404995242 2216 100-101 -0.7139845392179964 2216 102-103 -0.6525679176312309 2216 104-105 -0.6403958791752764 2216 106-107 -0.7563643479372182 2216 108-109 -0.8058362489406541 2216 110-111 -0.7331983892002505 2216 112-113 -0.7669294943676732 2216 114-115 -0.8343109660699852 2216 116-117 -0.7577250445092076 2216 118-119 -0.86110057096678 2216 120-121 -0.9458145099557917 2216 122-123 -0.8821590314014749 2216 124-125 -0.9839294865160397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36281.0 3 6831.0 4 4561.0 5 3938.0 6 4569.0 7 5308.0 8 5891.0 9 6742.0 10 7641.0 11 8840.0 12 9363.0 13 9678.0 14 9417.0 15 9134.0 16 8272.0 17 7524.0 18 6922.0 19 6545.0 20 6727.0 21 7230.0 22 8472.0 23 11232.0 24 15913.0 25 22327.0 26 26468.0 27 28570.0 28 34319.0 29 42569.0 30 51354.0 31 64189.0 32 83095.0 33 109505.0 34 150927.0 35 219574.0 36 360274.0 37 490901.0 38 149860.0 39 256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.98449445303142 17.760734468120546 9.40968692512511 22.845084153722922 2 19.022032008434206 20.436632745643273 37.64351767479321 22.89781757112931 3 17.472517620615545 28.012553295411514 30.272675565606207 24.242253518366738 4 11.040619084385325 15.819308958224736 37.45282205439132 35.687249902998616 5 11.207242021597121 42.031542260966354 35.023010996483436 11.738204720953087 6 31.238034023056983 36.92771521121848 19.0917713271187 12.74247943860584 7 28.81743956245303 33.6407835006697 21.62568256484672 15.916094372030543 8 27.895527660076887 33.356408097037466 19.886135104456173 18.861929138429474 9 28.312460592628085 12.788246400732126 18.754994050007372 40.14429895663242 10-11 24.50905296317208 26.317759436013194 29.289296767927414 19.88389083288731 12-13 25.33052057296438 25.14890940213818 28.593299738287868 20.92727028660957 14-15 23.08107607597064 26.516137661140228 25.478711338716227 24.924074924172906 16-17 23.04857200457288 28.915759158186834 25.974008477019623 22.061660360220667 18-19 22.250925880790106 27.988728982891875 27.50390769582755 22.256437440490465 20-21 23.71720912914502 27.253662754665864 27.070678120965823 21.95844999522329 22-23 23.355220250383717 27.522471276486193 26.811800301088134 22.310508172041956 24-25 22.799829117697634 27.56422661305692 27.01784906801047 22.61809520123498 26-27 22.80149658237572 27.764155509743365 26.850470119303303 22.58387778857761 28-29 22.64193463995932 27.596946089185405 27.087340399160098 22.67377887169518 30-31 22.80942282975409 27.35470924702557 27.17572065139405 22.660147271826283 32-33 22.446749486509628 27.680542236037773 27.146340776341106 22.72636750111149 34-35 22.917046856632233 27.672105720449476 26.892562173766386 22.518285249151905 36-37 23.023483677533612 27.284030756342453 26.83824015716207 22.854245408961862 38-39 22.65167967948156 27.535220824292463 27.096041383641822 22.71705811258416 40-41 22.76282243477818 27.410238547355753 27.313285204498555 22.513653813367508 42-43 22.658069370995126 27.338635973377222 27.18989880630585 22.8133958493218 44-45 22.59580713938577 27.548863796698384 27.1523047407779 22.703024323137942 46-47 22.770625589732987 27.51496929714116 26.85326806383373 22.861137049292125 48-49 22.5790095090657 27.508830546587564 26.96045482826363 22.951705116083108 50-51 22.674287724321125 27.397398522868933 27.05534256668854 22.872971186121404 52-53 22.74183197056619 27.457683998059956 27.087556768355714 22.71292726301814 54-55 22.578540123242075 27.565052866323793 27.167440889757227 22.688966120676902 56-57 22.668239606484164 27.591890704851608 27.032608698314732 22.707260990349496 58-59 22.880871213652178 27.06206672207529 27.036640483519854 23.020421580752682 60-61 22.745867004377338 27.477580534931747 27.32761936022771 22.448933100463208 62-63 22.553843071174533 27.431349364695485 27.27636597635738 22.738441587772602 64-65 22.428114937714106 27.575179705000874 27.216810761356413 22.779894595928603 66-67 22.54149931278456 27.812004506154075 26.925029951619344 22.72146622944202 68-69 22.64880994664442 27.388422852904476 27.15718757149555 22.805579628955556 70-71 22.424183783823505 27.31718190625379 27.335700402091412 22.922933907831293 72-73 22.63798535478659 27.33778885398045 27.031083968209096 22.99314182302386 74-75 22.371790443800705 27.692338217203627 27.175046749254978 22.76082458974069 76-77 22.29939876274215 27.547199390556816 27.283703396151544 22.869698450549492 78-79 22.480587494672278 27.484776186673592 27.065711024343404 22.968925294310726 80-81 22.494582862002726 27.528540711451605 26.953390812396783 23.023485614148882 82-83 22.70543968085651 27.580897343663462 26.943310927543834 22.77035204793619 84-85 22.389171128889238 27.296848437936323 27.196393315729395 23.117587117445044 86-87 22.252822286405333 27.460839987683766 27.281730406276477 23.004607319634424 88-89 22.444642421038733 27.390687811350787 27.077466194625583 23.087203572984897 90-91 22.413429475583495 27.644807997603383 27.146403006253884 22.795359520559234 92-93 22.480568506074462 27.383568418626307 27.233216384697407 22.90264669060182 94-95 22.106618133362073 27.261697718510096 27.468756276914185 23.162927871213643 96-97 22.466331782860667 27.44400306583536 27.27491181069226 22.814753340611713 98-99 22.34707757397402 27.387307457623717 27.056253250222355 23.20936171817991 100-101 22.21802807655282 27.341962223294576 27.26622264789671 23.173787052255896 102-103 22.408257127935904 27.185338581242156 27.214512682283015 23.191891608538924 104-105 22.205862072259716 27.220910770174406 27.357545647635355 23.215681509930526 106-107 22.09087279327652 27.322341460068927 27.379121646282467 23.20766410037208 108-109 22.339615098772832 27.49709851905669 26.897034537053955 23.26625184511652 110-111 22.17813308197706 27.597806772803946 27.034190299496423 23.189869845722573 112-113 22.345210456362338 27.410669275580574 27.18210352992127 23.062016738135817 114-115 22.025837679213993 27.599040762686744 27.026217356904375 23.34890420119488 116-117 22.00610767726753 27.604673032035297 27.18254420251994 23.206675088177235 118-119 22.101290498158306 27.522494860224818 26.881299791763475 23.49491484985341 120-121 22.072269690180025 27.63539023462407 26.987854947196542 23.304485127999367 122-123 22.243772721004134 27.507317385038853 26.868281680315086 23.380628213641923 124-125 22.434441378448785 27.42717239673086 26.647437852349654 23.490948372470708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 12.0 2 12.5 3 16.5 4 19.5 5 18.0 6 19.0 7 26.0 8 35.0 9 41.0 10 43.0 11 55.5 12 72.5 13 79.0 14 98.0 15 141.5 16 198.0 17 263.0 18 351.5 19 472.0 20 654.0 21 963.5 22 1442.0 23 2273.0 24 3476.5 25 4981.0 26 7028.0 27 9569.5 28 11879.5 29 15044.5 30 20064.5 31 25255.0 32 30985.0 33 36839.0 34 43461.0 35 49887.0 36 56291.5 37 63425.5 38 66738.5 39 67851.5 40 68911.0 41 71770.0 42 73989.5 43 75203.0 44 79304.0 45 82351.5 46 82743.5 47 81629.5 48 80603.5 49 78038.0 50 75019.0 51 72228.0 52 68271.0 53 66228.0 54 64359.5 55 60071.0 56 54782.0 57 49571.5 58 44712.5 59 39546.5 60 33198.5 61 27259.0 62 21817.0 63 16581.5 64 12429.0 65 9546.5 66 7372.0 67 5771.5 68 4555.5 69 3265.0 70 2381.5 71 1827.0 72 1422.0 73 1131.5 74 925.5 75 777.5 76 517.0 77 308.0 78 224.5 79 155.5 80 99.0 81 63.0 82 40.0 83 25.5 84 19.0 85 18.5 86 13.5 87 8.5 88 6.5 89 4.5 90 5.5 91 3.0 92 2.5 93 4.0 94 4.5 95 3.0 96 1.0 97 1.5 98 1.5 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.858646720415595E-4 2 3.429323360207797E-4 3 9.79806674345085E-5 4 5.388936708897968E-4 5 0.0010287970080623393 6 7.348550057588138E-4 7 0.0010287970080623393 8 0.001371729344083119 9 0.00195961334869017 10-11 0.00178814718067978 12-13 0.002204565017276441 14-15 0.0030374006904697634 16-17 0.0026209828538731024 18-19 0.002669973187590357 20-21 0.003135381357904272 22-23 0.09548216041492853 24-25 0.002351536018428204 26-27 0.0017146616801038987 28-29 0.0016166810126693903 30-31 0.001347234177224492 32-33 0.0020330988492660513 34-35 0.0012002631760727291 36-37 6.858646720415595E-4 38-39 0.0012737486766486103 40-41 0.0015187003452348817 42-43 0.0016901665132452717 44-45 0.0017146616801038987 46-47 0.0020086036824074243 48-49 4.409130034552882E-4 50-51 0.001592185845810763 52-53 0.0014207196778003733 54-55 0.0016166810126693903 56-57 0.0014942051783762545 58-59 0.0010043018412037121 60-61 0.001175768009214102 62-63 0.001592185845810763 64-65 0.0012982438435072377 66-67 5.143985040311697E-4 68-69 3.91922669738034E-4 70-71 8.083405063346951E-4 72-73 2.939420023035255E-4 74-75 4.899033371725425E-4 76-77 5.633888377484239E-4 78-79 4.409130034552882E-4 80-81 5.87884004607051E-4 82-83 2.694468354448984E-4 84-85 4.409130034552882E-4 86-87 5.143985040311697E-4 88-89 6.12379171465678E-4 90-91 3.674275028794069E-4 92-93 4.654081703139153E-4 94-95 4.409130034552882E-4 96-97 6.613695051829324E-4 98-99 6.12379171465678E-4 100-101 6.858646720415595E-4 102-103 0.001224758342931356 104-105 6.12379171465678E-4 106-107 6.858646720415595E-4 108-109 9.79806674345085E-4 110-111 0.0012737486766486103 112-113 5.87884004607051E-4 114-115 6.858646720415595E-4 116-117 8.573308400519493E-4 118-119 0.0014942051783762545 120-121 8.573308400519493E-4 122-123 9.063211737692035E-4 124-125 5.633888377484239E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2041219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.87842743833833 #Duplication Level Percentage of deduplicated Percentage of total 1 48.213058884858505 15.369564992412949 2 16.993943705790034 10.83480402632468 3 11.491456282896117 10.989886658254228 4 7.492856133060003 9.554418821746463 5 4.734825856673194 7.546940125256225 6 3.0804810022184084 5.892053406263955 7 1.9509230262053978 4.353465069007053 8 1.302656368822555 3.3221309224479287 9 0.8910430280856461 2.5564545463738977 >10 3.5425328626097583 18.985760799675536 >50 0.2013728287446939 4.419954635256093 >100 0.10190817101821172 5.592429548519329 >500 0.0027870148587358368 0.5301123839645606 >1k 1.5483415881865751E-4 0.0520240644970807 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 2 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 3 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 4 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 5 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 6 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 7 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 8 0.0 0.0 0.0 1.9596133486901699E-4 4.8990333717254247E-5 9 0.0 0.0 0.0 2.939420023035255E-4 4.8990333717254247E-5 10-11 0.0 0.0 0.0 2.939420023035255E-4 4.8990333717254247E-5 12-13 0.0 0.0 0.0 2.939420023035255E-4 4.8990333717254247E-5 14-15 0.0 0.0 0.0 2.939420023035255E-4 4.8990333717254247E-5 16-17 0.0 0.0 0.0 2.939420023035255E-4 4.8990333717254247E-5 18-19 0.0 0.0 0.0 3.184371691621526E-4 4.8990333717254247E-5 20-21 0.0 0.0 0.0 3.429323360207797E-4 4.8990333717254247E-5 22-23 0.0 0.0 0.0 3.429323360207797E-4 4.8990333717254247E-5 24-25 0.0 0.0 0.0 3.429323360207797E-4 4.8990333717254247E-5 26-27 0.0 0.0 0.0 3.9192266973803397E-4 4.8990333717254247E-5 28-29 0.0 0.0 0.0 6.368743383243053E-4 4.8990333717254247E-5 30-31 0.0 0.0 0.0 0.0016166810126693903 4.8990333717254247E-5 32-33 0.0 0.0 0.0 0.00536444154203934 4.8990333717254247E-5 34-35 0.0 0.0 0.0 0.010410445914916529 4.8990333717254247E-5 36-37 0.0 0.0 0.0 0.019547143153184447 4.8990333717254247E-5 38-39 0.0 0.0 0.0 0.03813897479888243 4.8990333717254247E-5 40-41 0.0 0.0 0.0 0.06148286881515408 4.8990333717254247E-5 42-43 0.0 0.0 0.0 0.08830507652535077 4.8990333717254247E-5 44-45 0.0 0.0 0.0 0.12377407813664285 4.8990333717254247E-5 46-47 0.0 0.0 0.0 0.16727749447756463 4.8990333717254247E-5 48-49 0.0 0.0 0.0 0.2124220869980144 4.8990333717254247E-5 50-51 0.0 0.0 0.0 0.26114297387982377 4.8990333717254247E-5 52-53 0.0 0.0 0.0 0.3136851067915789 4.8990333717254247E-5 54-55 0.0 0.0 0.0 0.3798465524767308 4.8990333717254247E-5 56-57 0.0 0.0 0.0 0.4688374936741232 4.8990333717254247E-5 58-59 0.0 0.0 0.0 0.5718396703146502 4.8990333717254247E-5 60-61 0.0 0.0 0.0 0.6718779317652834 4.8990333717254247E-5 62-63 0.0 0.0 0.0 0.7712793188775923 4.8990333717254247E-5 64-65 0.0 0.0 0.0 0.8897134506390544 4.8990333717254247E-5 66-67 0.0 0.0 0.0 1.0371008696274138 4.8990333717254247E-5 68-69 0.0 0.0 0.0 1.2163320055319886 4.8990333717254247E-5 70-71 0.0 0.0 0.0 1.4007561168105922 4.8990333717254247E-5 72-73 0.0 0.0 0.0 1.5777826876978902 4.8990333717254247E-5 74-75 0.0 0.0 0.0 1.7809700967902025 4.8990333717254247E-5 76-77 0.0 0.0 0.0 2.016368650301609 4.8990333717254247E-5 78-79 0.0 0.0 0.0 2.292992569636085 4.8990333717254247E-5 80-81 0.0 0.0 0.0 2.586861086439035 4.8990333717254247E-5 82-83 0.0 0.0 0.0 2.8813174872465916 4.8990333717254247E-5 84-85 0.0 0.0 0.0 3.2017387649242925 4.8990333717254247E-5 86-87 0.0 0.0 0.0 3.5595396672282593 4.8990333717254247E-5 88-89 0.0 0.0 0.0 3.9757615424900514 4.8990333717254247E-5 90-91 0.0 0.0 0.0 4.423925115335493 4.8990333717254247E-5 92-93 0.0 0.0 0.0 4.867777538813817 4.8990333717254247E-5 94-95 0.0 0.0 0.0 5.324612400727212 4.8990333717254247E-5 96-97 0.0 0.0 0.0 5.829629255851528 4.8990333717254247E-5 98-99 0.0 0.0 0.0 6.387751632725347 4.8990333717254247E-5 100-101 0.0 0.0 0.0 6.989377425940088 4.8990333717254247E-5 102-103 0.0 0.0 0.0 7.591125694989122 4.8990333717254247E-5 104-105 0.0 0.0 0.0 8.220749463923273 4.8990333717254247E-5 106-107 0.0 0.0 0.0 8.888022304319135 4.8990333717254247E-5 108-109 0.0 0.0 0.0 9.58860857164273 4.8990333717254247E-5 110-111 0.0 0.0 0.0 10.343500623891899 4.8990333717254247E-5 112-113 0.0 0.0 0.0 11.088006725393011 4.8990333717254247E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16040 0.0 54.188976 1 GGTATCA 2860 0.0 53.25238 1 TACATGG 16605 0.0 51.91521 2 ACATGGG 16935 0.0 49.955063 3 CATGGGA 11280 0.0 46.360157 4 CATGGGG 5155 0.0 41.893154 4 GTATCAA 4055 0.0 40.493317 1 ATGGGAT 4600 0.0 40.481033 5 ATGGGAG 4395 0.0 37.902184 5 TCAACGC 4780 0.0 32.858 4 ATGGGGG 2870 0.0 32.337605 5 ATCAACG 4895 0.0 31.96452 3 CAACGCA 4935 0.0 31.825987 5 GAGTACA 9920 0.0 30.286203 1 AGTACAT 9435 0.0 30.014433 2 AACGCAG 5305 0.0 29.830555 6 GTCGGGA 450 0.0 27.763351 2 TATCAAC 5870 0.0 26.756601 3 TACCTGG 1925 0.0 26.578646 2 TGGGATA 1665 0.0 26.083971 6 >>END_MODULE