FastQCFastQC Report
Thu 26 May 2016
SRR936217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041219
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.11978136593868663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.070.4556661
GTATCAA60150.048.099712
GTACATG146700.046.2568631
TACATGG152600.044.0434382
ACATGGG150500.043.866893
CATGGGA101050.038.7043884
CATGGGG46950.038.1647154
TCAACGC80450.037.58975
ATCAACG81300.037.3431364
CAACGCA82750.036.594426
AACGCAG85450.035.577387
TATCAAC89850.034.0554663
ATGGGAT42450.033.235475
ATGGGAG42500.030.2549155
ACGCAGA101200.029.4525138
CGCAGAG103050.028.9814999
AGTACAT91850.028.3881262
TACCTGG17900.027.9362452
GAGTACA98300.027.7584081
ATGGGGG26700.026.7547655
Produced by FastQC (version 0.11.5)