FastQCFastQC Report
Thu 26 May 2016
SRR936216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972930
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.069.255311
GTACATG191450.056.836341
TACATGG193000.055.7325442
ACATGGG197400.052.5312843
CATGGGA131150.049.0825084
GTATCAA47100.046.9883042
CATGGGG61250.046.3318944
ATGGGAG49950.041.6869665
ATGGGGG40250.040.7953765
ATGGGAT55050.040.5267455
TCAACGC56900.039.7318765
ATCAACG57250.039.4889764
CAACGCA57650.039.421386
AACGCAG61050.037.518277
TATCAAC67850.033.3197333
AGTACAT105450.033.230472
GAGTACA109900.033.184141
TACCTGG16550.032.7123072
CATGGGT18350.031.7729664
ATGGGAA49100.031.7458955
Produced by FastQC (version 0.11.5)