##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1972930 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.771085137333813 31.0 30.0 33.0 26.0 34.0 2 29.8994054528037 31.0 30.0 34.0 26.0 34.0 3 30.130050736721525 31.0 30.0 34.0 26.0 34.0 4 33.5512790621056 37.0 35.0 37.0 28.0 37.0 5 33.90145823724106 37.0 35.0 37.0 30.0 37.0 6 33.83340311110886 37.0 35.0 37.0 29.0 37.0 7 33.703297633469006 37.0 35.0 37.0 28.0 37.0 8 33.70417804990547 37.0 35.0 37.0 28.0 37.0 9 35.06294496003406 39.0 35.0 39.0 27.0 39.0 10-11 34.93203585530151 39.0 35.0 39.0 27.0 39.0 12-13 34.93214888516064 39.0 35.0 39.0 27.0 39.0 14-15 36.08389476565311 40.0 36.0 41.0 27.0 41.0 16-17 36.13347305783783 40.0 36.0 41.0 27.0 41.0 18-19 36.18502607796526 40.0 36.0 41.0 27.0 41.0 20-21 36.2985501259548 40.0 36.0 41.0 27.0 41.0 22-23 36.22387743102898 40.0 36.0 41.0 27.0 41.0 24-25 36.227790392968835 40.0 36.0 41.0 27.5 41.0 26-27 36.33697698347129 40.0 36.0 41.0 29.0 41.0 28-29 36.24615191618557 40.0 36.0 41.0 29.0 41.0 30-31 36.16977287587497 39.5 35.0 41.0 29.0 41.0 32-33 35.99746088305211 39.0 35.0 41.0 28.0 41.0 34-35 35.9698410485927 39.0 35.0 41.0 28.0 41.0 36-37 35.87294354082506 39.0 35.0 41.0 28.0 41.0 38-39 35.63869701408565 39.0 35.0 41.0 27.0 41.0 40-41 35.744047431992016 39.0 35.0 41.0 27.5 41.0 42-43 35.66928020761 39.0 35.0 41.0 27.0 41.0 44-45 35.571507098579275 39.0 35.0 41.0 27.0 41.0 46-47 35.5542991895303 39.0 35.0 41.0 27.0 41.0 48-49 35.46924776854729 39.0 34.5 41.0 27.0 41.0 50-51 35.345420516693444 39.0 34.0 41.0 27.0 41.0 52-53 35.199712610178764 39.0 34.0 41.0 27.0 41.0 54-55 35.0308366237018 38.5 34.0 40.0 27.0 41.0 56-57 34.84796166108275 38.0 34.0 40.0 26.0 41.0 58-59 34.62181881769753 38.0 33.0 40.0 26.0 41.0 60-61 34.38240865109253 38.0 33.0 40.0 26.0 41.0 62-63 34.125766246141524 37.5 33.0 40.0 25.0 41.0 64-65 33.844335582103774 37.0 33.0 40.0 24.5 41.0 66-67 33.549431809542156 36.5 32.5 40.0 23.5 41.0 68-69 33.253147095943596 36.0 32.0 39.0 23.0 41.0 70-71 32.900544114590986 35.5 32.0 39.0 22.5 41.0 72-73 32.5756293938457 35.0 32.0 38.5 22.0 40.0 74-75 32.23317755825093 35.0 31.5 37.5 22.0 40.0 76-77 31.89948680389066 35.0 31.0 37.0 20.5 39.0 78-79 31.55924716031486 35.0 31.0 36.5 20.0 39.0 80-81 31.240796683105838 35.0 31.0 36.0 19.5 38.5 82-83 30.941483225456555 35.0 31.0 36.0 18.5 37.0 84-85 30.960642293441737 35.0 31.0 35.5 20.0 37.0 86-87 30.83500732413213 35.0 31.0 35.0 19.0 37.0 88-89 30.65957484553431 35.0 31.0 35.0 18.0 36.0 90-91 30.490652988195222 35.0 31.0 35.0 18.0 36.0 92-93 30.35551565438206 35.0 31.0 35.0 17.0 36.0 94-95 30.224016817626577 34.0 31.0 35.0 14.0 35.5 96-97 30.118969755642624 34.0 31.0 35.0 10.5 35.0 98-99 30.0204221639896 34.0 31.0 35.0 8.5 35.0 100-101 29.924800423735256 34.0 31.0 35.0 7.0 35.0 102-103 28.961866361198826 33.5 29.0 34.5 6.0 35.0 104-105 29.6237040847876 34.0 30.0 35.0 2.0 35.0 106-107 29.73431723375893 34.0 31.0 35.0 2.0 35.0 108-109 29.69063322064138 34.0 31.0 35.0 2.0 35.0 110-111 29.58699244271211 34.0 30.0 35.0 2.0 35.0 112-113 29.55066626793652 34.0 31.0 35.0 2.0 35.0 114-115 29.43582184872246 34.0 30.0 35.0 2.0 35.0 116-117 29.318681605530863 34.0 30.0 35.0 2.0 35.0 118-119 29.12485288378199 34.0 30.0 35.0 2.0 35.0 120-121 28.936629784128172 34.0 30.0 35.0 2.0 35.0 122-123 28.661730015763357 34.0 30.0 35.0 2.0 35.0 124-125 26.66754091630215 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6241983475162449 1101 2 0.6332517452828696 1101 3 0.6511189356198273 1101 4 0.7397836639012425 1101 5 0.6215891917201048 1101 6 0.6063003939489491 1101 7 0.5688066255821695 1101 8 0.6390732766756528 1101 9 0.7459933721257812 1101 10-11 0.7795449463635151 1101 12-13 0.7168194495594662 1101 14-15 0.7873707771944467 1101 16-17 0.7481284285328726 1101 18-19 0.6970558588156308 1101 20-21 0.6530059733852056 1101 22-23 0.7203760779386315 1101 24-25 0.7672518885377997 1101 26-27 0.605916096353809 1101 28-29 0.7036641400460653 1101 30-31 0.7151594219810775 1101 32-33 0.7866393058531571 1101 34-35 0.7556841450132197 1101 36-37 0.6929301787737643 1101 38-39 0.7084751821398925 1101 40-41 0.695406645230058 1101 42-43 0.8935854403594377 1101 44-45 0.791428879136383 1101 46-47 0.6486527785306464 1101 48-49 0.7412003370818283 1101 50-51 0.7087944101920058 1101 52-53 0.7237269623904723 1101 54-55 0.7345621336531281 1101 56-57 0.7906353899370799 1101 58-59 0.7360619838807168 1101 60-61 0.7736257271498204 1101 62-63 0.8723758398573693 1101 64-65 0.8254964382266721 1101 66-67 0.8511289866740483 1101 68-69 0.7459679230910723 1101 70-71 0.7559425388148924 1101 72-73 0.7594430731516653 1101 74-75 0.6570349735148611 1101 76-77 0.7099997360273917 1101 78-79 0.6470052898190382 1101 80-81 0.6437309108888343 1101 82-83 0.6669413952759911 1101 84-85 0.5375328505901571 1101 86-87 0.6622319808553812 1101 88-89 0.6113732070110913 1101 90-91 0.5830783457814555 1101 92-93 0.6517310235753229 1101 94-95 0.6657071875300353 1101 96-97 0.6160433189150787 1101 98-99 0.6069248113357517 1101 100-101 0.5435436156716023 1101 102-103 0.6469845089861579 1101 104-105 0.7439821723195585 1101 106-107 0.5772108114574017 1101 108-109 0.520454212674629 1101 110-111 0.6012420764386164 1101 112-113 0.6412202900673734 1101 114-115 0.6702685537538997 1101 116-117 0.7400923612878216 1101 118-119 0.7264106345272978 1101 120-121 0.7446244218521478 1101 122-123 0.6806645876509236 1101 124-125 0.45714940151189154 1102 1 0.3487308369374986 1102 2 0.4229995592618998 1102 3 0.4302372124590228 1102 4 0.530677205738165 1102 5 0.41394503697249974 1102 6 0.4943818722710631 1102 7 0.4440656542801733 1102 8 0.456492420760398 1102 9 0.6106689879938401 1102 10-11 0.7556078403872206 1102 12-13 0.5618045975316761 1102 14-15 0.7953735620174882 1102 16-17 0.6903523039950628 1102 18-19 0.6270517998763552 1102 20-21 0.5358821334115973 1102 22-23 0.7824404609635849 1102 24-25 0.8227051928290976 1102 26-27 0.7795238747574302 1102 28-29 0.7669146775299396 1102 30-31 0.7962800365088185 1102 32-33 0.8611442834801579 1102 34-35 0.7390585018504225 1102 36-37 0.7040683856559511 1102 38-39 0.8137624931794534 1102 40-41 0.7214598260167335 1102 42-43 0.8420985553999714 1102 44-45 0.8213466259258624 1102 46-47 0.7372055930360375 1102 48-49 0.7372117238173459 1102 50-51 0.6384025272023308 1102 52-53 0.6657157123999724 1102 54-55 0.6231982432358052 1102 56-57 0.7029257820805412 1102 58-59 0.7491564041415444 1102 60-61 0.8569293238692381 1102 62-63 0.8392510969325713 1102 64-65 0.7169526966443627 1102 66-67 0.7189895132725823 1102 68-69 0.7192794206334554 1102 70-71 0.6382655869697516 1102 72-73 0.6864683915993268 1102 74-75 0.5904998425272439 1102 76-77 0.6665321321147459 1102 78-79 0.7028555887588332 1102 80-81 0.5840620074644569 1102 82-83 0.5075934085207194 1102 84-85 0.522023090143545 1102 86-87 0.5673522676292109 1102 88-89 0.5593836213376271 1102 90-91 0.5420946173527099 1102 92-93 0.588865705474948 1102 94-95 0.5557112356165241 1102 96-97 0.5130859909560392 1102 98-99 0.5438494919656058 1102 100-101 0.5976889973258501 1102 102-103 0.5698410037773662 1102 104-105 0.5220286287244882 1102 106-107 0.5481164649954593 1102 108-109 0.5733545092810708 1102 110-111 0.5954922742305087 1102 112-113 0.5394028294656756 1102 114-115 0.6359863619701045 1102 116-117 0.6941008351375118 1102 118-119 0.6772054920465891 1102 120-121 0.5856939414973041 1102 122-123 0.6494104296800458 1102 124-125 0.5391480601084311 1103 1 0.2186902040763279 1103 2 0.3195182996432031 1103 3 0.31568807590732106 1103 4 0.35372916086762274 1103 5 0.3864173634653909 1103 6 0.3294758902593884 1103 7 0.28267275805528413 1103 8 0.2591087061670052 1103 9 0.32049717028367297 1103 10-11 0.5234088047637044 1103 12-13 0.3162689805617944 1103 14-15 0.41122179479321375 1103 16-17 0.4381037634210543 1103 18-19 0.48360984339537083 1103 20-21 0.36702338492116127 1103 22-23 0.5552420575036905 1103 24-25 0.6018053188258463 1103 26-27 0.5365456865819382 1103 28-29 0.5116513704858932 1103 30-31 0.5015122803408687 1103 32-33 0.5910782114832642 1103 34-35 0.5712724696144278 1103 36-37 0.6050454054186929 1103 38-39 0.6742121576967577 1103 40-41 0.6507697649366477 1103 42-43 0.6646143703896641 1103 44-45 0.6340330596947368 1103 46-47 0.48822871714501304 1103 48-49 0.4746394064799304 1103 50-51 0.5183525862769329 1103 52-53 0.5171830949374296 1103 54-55 0.5655149484862534 1103 56-57 0.5878798521242601 1103 58-59 0.5519257167399161 1103 60-61 0.5686247866783987 1103 62-63 0.6229227467923977 1103 64-65 0.523811017879396 1103 66-67 0.5007184589544664 1103 68-69 0.5368061567945261 1103 70-71 0.4929938438112913 1103 72-73 0.5114554213458931 1103 74-75 0.496268226499069 1103 76-77 0.3766207004167583 1103 78-79 0.45913580726945824 1103 80-81 0.5211970419718703 1103 82-83 0.5141637683308105 1103 84-85 0.5079775046074495 1103 86-87 0.4548400058349955 1103 88-89 0.513787948824536 1103 90-91 0.5505381433433563 1103 92-93 0.5232704471380174 1103 94-95 0.5418558441328614 1103 96-97 0.5925958243700258 1103 98-99 0.5207732312351716 1103 100-101 0.5658291501786472 1103 102-103 0.6052022743826306 1103 104-105 0.6864209233040128 1103 106-107 0.624255776416696 1103 108-109 0.6787117689172071 1103 110-111 0.6685987262231308 1103 112-113 0.6841051893978367 1103 114-115 0.5792280969758394 1103 116-117 0.561183203876368 1103 118-119 0.5618198286453762 1103 120-121 0.5196552966817016 1103 122-123 0.6035479405249227 1103 124-125 0.6974798675295943 1104 1 0.24436123030174528 1104 2 0.25706607060617515 1104 3 0.16448915750912363 1104 4 0.25148087455269774 1104 5 0.26609707528102433 1104 6 0.19026312104575993 1104 7 0.1448960983354084 1104 8 0.27279109033854354 1104 9 0.3157698229870931 1104 10-11 0.3961030090376525 1104 12-13 0.3994631828848014 1104 14-15 0.37620657311643413 1104 16-17 0.41192202414396917 1104 18-19 0.4570186774305398 1104 20-21 0.431545562570399 1104 22-23 0.48186038490543837 1104 24-25 0.42609244491170983 1104 26-27 0.3762657872158215 1104 28-29 0.33762914969917546 1104 30-31 0.27707508352254706 1104 32-33 0.5349908739153761 1104 34-35 0.5028349697499124 1104 36-37 0.426140412353206 1104 38-39 0.5615460621500645 1104 40-41 0.592782698376169 1104 42-43 0.6137880709219274 1104 44-45 0.5909812067124633 1104 46-47 0.48086060835706235 1104 48-49 0.4583591815233987 1104 50-51 0.4928808270178706 1104 52-53 0.49331089069036693 1104 54-55 0.4319125984258392 1104 56-57 0.4261952750948197 1104 58-59 0.3969488007097226 1104 60-61 0.4377078529856888 1104 62-63 0.41846313365970644 1104 64-65 0.37812123227048744 1104 66-67 0.4290684092488206 1104 68-69 0.3745950966053897 1104 70-71 0.39195909711499155 1104 72-73 0.4643593756435891 1104 74-75 0.35152161257428816 1104 76-77 0.3090462210687761 1104 78-79 0.3000363357143989 1104 80-81 0.36024135825152115 1104 82-83 0.38366563668216713 1104 84-85 0.3074695228734612 1104 86-87 0.3048286686989172 1104 88-89 0.36469751012722895 1104 90-91 0.3636996478918668 1104 92-93 0.4111632962448546 1104 94-95 0.4170043324352335 1104 96-97 0.43409876010299087 1104 98-99 0.43027443409880917 1104 100-101 0.39249002977566505 1104 102-103 0.4486961456092402 1104 104-105 0.46611170129167334 1104 106-107 0.5493722584448406 1104 108-109 0.570750259686438 1104 110-111 0.4618808986525167 1104 112-113 0.37322319706200346 1104 114-115 0.3538545025747766 1104 116-117 0.3678074296236744 1104 118-119 0.385048027202636 1104 120-121 0.41821860190558624 1104 122-123 0.446147792947059 1104 124-125 0.39354777805856145 1105 1 0.01612451277718918 1105 2 0.08685461293882568 1105 3 0.08336358604446303 1105 4 0.05336444705177712 1105 5 0.006353852184879827 1105 6 -0.1420568151320225 1105 7 -0.08116234094759989 1105 8 -0.06219025692369229 1105 9 -0.030501804351430906 1105 10-11 0.010123524138755613 1105 12-13 0.02257712592931682 1105 14-15 0.008856330803887147 1105 16-17 -0.16051689288915583 1105 18-19 -0.12475417562606594 1105 20-21 -0.2120048997289885 1105 22-23 -0.2132173328067637 1105 24-25 0.05889063497860292 1105 26-27 -0.06401132042606861 1105 28-29 -0.04805664546498889 1105 30-31 0.0657141485752959 1105 32-33 0.1558016015176662 1105 34-35 0.11498296143601294 1105 36-37 0.0434351182015007 1105 38-39 0.21139995282996438 1105 40-41 0.001613784656711914 1105 42-43 0.1486883598173705 1105 44-45 0.1872235973701848 1105 46-47 -0.028427977738004984 1105 48-49 0.061687273597414105 1105 50-51 0.11337780931616237 1105 52-53 0.003869592283280099 1105 54-55 0.12629662627630722 1105 56-57 0.14504032407327117 1105 58-59 0.19662996478364647 1105 60-61 0.1836813138054083 1105 62-63 0.24089562126890485 1105 64-65 0.20714025458106988 1105 66-67 0.33929867575928796 1105 68-69 0.1960711081866009 1105 70-71 0.13402203942893465 1105 72-73 0.0797590154053367 1105 74-75 0.07335483554025046 1105 76-77 -9.172587450052561E-4 1105 78-79 0.05731034252186262 1105 80-81 0.08828857975083793 1105 82-83 0.03459300303031654 1105 84-85 0.023979288665689324 1105 86-87 0.08948805263628401 1105 88-89 0.06680420215739957 1105 90-91 0.062449863132044925 1105 92-93 0.05124774342075966 1105 94-95 0.02156787932668891 1105 96-97 0.08997085615974854 1105 98-99 0.07321407466702468 1105 100-101 0.10268493710041326 1105 102-103 0.14558288625326554 1105 104-105 0.10331359695043218 1105 106-107 0.12631087263358154 1105 108-109 0.20685232697642064 1105 110-111 0.33009241918534826 1105 112-113 0.16254520981141596 1105 114-115 0.1105664771889785 1105 116-117 0.13802145906059593 1105 118-119 0.18503087148875963 1105 120-121 0.22782811944267323 1105 122-123 0.2426682204704136 1105 124-125 0.12453364316851179 1106 1 -0.04575745247115037 1106 2 -0.14051186434423002 1106 3 0.1117362319261872 1106 4 0.06866448272673153 1106 5 0.10243692964002804 1106 6 0.17749757514570064 1106 7 0.010462873712263843 1106 8 0.005581242686069743 1106 9 0.08861098509210308 1106 10-11 0.2256402059426108 1106 12-13 0.07446782037570188 1106 14-15 -0.020912052051166086 1106 16-17 0.014046919833454297 1106 18-19 0.0028437993661611927 1106 20-21 0.053244739237399585 1106 22-23 0.05067837171117873 1106 24-25 -4.097687498613958 1106 26-27 -0.9651868281969271 1106 28-29 -0.5307830632586317 1106 30-31 -0.2838717467854508 1106 32-33 -5.01655218181855 1106 34-35 -0.7361898200832258 1106 36-37 -0.10476916476575582 1106 38-39 0.21301872315027026 1106 40-41 -0.3404221592832428 1106 42-43 0.035199749577365935 1106 44-45 -0.013450332030807033 1106 46-47 -0.051498349822011846 1106 48-49 0.06179679101971658 1106 50-51 0.07976188052896305 1106 52-53 0.12082962958879051 1106 54-55 -0.10129833685698486 1106 56-57 0.07449272138316587 1106 58-59 0.16189217047180193 1106 60-61 0.1283400156630563 1106 62-63 0.10932651027100349 1106 64-65 0.21042697160809354 1106 66-67 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-1.0491976601551087 2216 50-51 -1.0097020820202047 2216 52-53 -1.1336104276443706 2216 54-55 -1.1232408743601638 2216 56-57 -1.12992016505288 2216 58-59 -1.0112299286093744 2216 60-61 -1.275388366104096 2216 62-63 -1.3210509394630208 2216 64-65 -1.2408490587633807 2216 66-67 -1.2022255803730104 2216 68-69 -1.063722347035565 2216 70-71 -1.2130397293203856 2216 72-73 -1.1496221988838649 2216 74-75 -1.1166725000940119 2216 76-77 -1.1204085743397947 2216 78-79 -0.9146831620202676 2216 80-81 -0.941631010753774 2216 82-83 -1.087454563768766 2216 84-85 -1.2290594794137206 2216 86-87 -1.1556468434274052 2216 88-89 -0.9843099895095264 2216 90-91 -0.9927969213367618 2216 92-93 -0.9548667327843567 2216 94-95 -1.0012817856670253 2216 96-97 -1.1364736244482252 2216 98-99 -0.9792180779492803 2216 100-101 -1.0059761060265906 2216 102-103 -1.014645912406511 2216 104-105 -0.9758001015169562 2216 106-107 -0.9860889524503094 2216 108-109 -1.0599824002612763 2216 110-111 -1.0208029775856957 2216 112-113 -1.0782054019457021 2216 114-115 -1.0792140590007833 2216 116-117 -0.9939924323935756 2216 118-119 -0.9991787562853602 2216 120-121 -1.1070300058200289 2216 122-123 -1.0782484817219284 2216 124-125 -1.1671588399314174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41302.0 3 6720.0 4 4585.0 5 3930.0 6 4856.0 7 5401.0 8 5635.0 9 6354.0 10 8008.0 11 9443.0 12 10281.0 13 10688.0 14 10425.0 15 9526.0 16 8577.0 17 7672.0 18 6992.0 19 6577.0 20 6229.0 21 6443.0 22 7965.0 23 10865.0 24 16922.0 25 25048.0 26 29166.0 27 26599.0 28 30124.0 29 38114.0 30 44885.0 31 55477.0 32 71787.0 33 96769.0 34 136991.0 35 195349.0 36 324674.0 37 490248.0 38 191679.0 39 624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.384708959309044 18.001338118119335 9.538957484337935 22.07499543823368 2 18.907095349749557 20.968703337073972 37.81256874307501 22.311632570101462 3 17.291448399815707 29.42341057382197 30.150147319036826 23.134993707325506 4 10.811645080208564 16.420390294786557 36.96031109234591 35.80765353265897 5 10.744978339026298 43.87762601607885 34.12838847120208 11.249007173692776 6 30.17526359486526 37.31941686256978 19.459388498434297 13.045931044130663 7 28.35781405464914 35.10899872422149 21.101133197459795 15.43205402366957 8 27.783544815798887 34.14515094039327 19.80224015635882 18.26906408744902 9 28.631196071941417 13.745467926886693 18.5679029116273 39.055433089544586 10-11 24.289067966691427 27.551674945150133 28.687245154369066 19.472011933789364 12-13 24.901527059213524 26.423340127134132 28.25829993456257 20.416832879089775 14-15 22.602731220168543 27.81411401601081 25.395511934610482 24.187642829210162 16-17 22.563369672983697 30.027325700452334 25.88348536242668 21.525819264137287 18-19 21.767126512929515 28.902874721313292 27.830223945375 21.49977482038219 20-21 23.427575046791386 27.996162916386297 27.26306198765493 21.313200049167385 22-23 23.010277489163432 28.267229938146336 27.020010106011654 21.70248246667857 24-25 22.403480813757813 28.1648530670008 27.42030400350352 22.011362115737867 26-27 22.183550759990116 28.320418675672084 27.258320608752403 22.237709955585405 28-29 22.084623636865437 28.475887005271712 27.220975741913445 22.218513615949405 30-31 22.5269014638137 27.884182352376165 27.57359808139766 22.015318102412472 32-33 22.185730223231687 28.305758628362653 27.277061032002404 22.231450116403252 34-35 22.57477041480942 28.314476209206113 27.47244420159906 21.638309174385398 36-37 22.57870353944174 28.19388618842218 27.073282612992987 22.154127659143093 38-39 22.211611786808305 28.33366406466013 27.341799548431357 22.112924600100207 40-41 22.437985676856957 28.089387604282244 27.44837398518215 22.024252733678647 42-43 22.200795587369136 28.18921760145817 27.20253922941159 22.407447581761097 44-45 22.076617706243542 28.154287678051343 27.452473178886162 22.316621436818952 46-47 22.194493428132187 28.17055683597423 27.059591591780336 22.57535814411325 48-49 22.23990045224787 28.13032442987497 27.216958598221673 22.412816519655486 50-51 22.165714637627058 28.03286445301224 27.546877261116485 22.254543648244216 52-53 22.31863423283053 27.826266784631848 27.555877811250806 22.299221171286813 54-55 21.91817031913327 27.959458469692063 27.729922162431215 22.392449048743455 56-57 22.19515823561956 28.28648132327266 27.289292671967335 22.22906776914045 58-59 22.441672953925064 28.042245307745358 27.23424918223402 22.281832556095566 60-61 22.522763398523193 28.061958412573546 27.629575488721198 21.785702700182068 62-63 22.106549005395625 28.06089528332729 27.650837981747635 22.18171772952945 64-65 21.90916440477367 27.957554976037873 27.775564459515284 22.35771615967317 66-67 22.0326049465222 28.52249823421763 27.236816061865614 22.208080757394555 68-69 22.252794457766473 28.089084064608706 27.385964885603748 22.272156592021076 70-71 22.014909391801734 27.961584810312644 27.611924979123557 22.41158081876206 72-73 21.920422722607295 28.44951024494089 27.265075965888215 22.364991066563604 74-75 21.848326338624982 28.80839060884374 27.33564969689597 22.007633355635303 76-77 21.674370983111327 28.367166433509723 27.486973622853434 22.471488960525516 78-79 21.97054269970427 28.38569260288095 27.27062149140603 22.37314320600875 80-81 22.06289521122663 28.393649705904938 27.05329901189432 22.490156070974113 82-83 22.078831137102963 28.546965268844342 27.142097574871027 22.232106019181664 84-85 21.825123079051902 28.43350385900283 27.302793475048897 22.43857958689637 86-87 21.764243295278902 28.48489256657456 27.25496402666201 22.49590011148453 88-89 21.85434609118024 28.282545155864426 27.412183941895158 22.450924811060176 90-91 21.940944416933064 28.358839507511956 27.448767588768124 22.251448486786852 92-93 22.114739096045227 28.23531319955021 27.379779053326807 22.270168651077757 94-95 21.815746110205232 28.22176176518144 27.434856676832652 22.527635447780675 96-97 21.873812036409088 28.25100333820937 27.381705436729632 22.493479188651914 98-99 21.438790175651302 28.294181020008725 27.277157727818413 22.989871076521563 100-101 21.483064775488245 28.46811151725024 27.385677382370094 22.66314632489142 102-103 21.694097479703768 28.2205714117724 27.359961437536462 22.725369670987373 104-105 21.54234606482332 28.136061510966126 27.43091256016157 22.890679864048984 106-107 21.438750355755587 28.351572433358076 27.512052606462472 22.697624604423865 108-109 21.749396641364843 28.439372309342346 27.152215668747935 22.659015380544876 110-111 21.459917502405084 28.94790730019575 27.03228131497395 22.559893882425214 112-113 21.599426432025528 28.731695535562 27.194983459741138 22.47389457267133 114-115 21.46380566054663 28.869532910372904 27.00574630788187 22.660915121198588 116-117 21.30754968310673 28.82317132511262 27.235435682628246 22.633843309152397 118-119 21.454963022623346 28.708717719717036 27.070491062501787 22.765828195157827 120-121 21.545634091445333 28.741062357016084 27.01551087976591 22.697792671772675 122-123 21.555785841853123 28.76598561563865 26.876463735103513 22.80176480740471 124-125 21.72004701667632 28.63883782237164 26.592781145887027 23.048334015065002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 274.0 2 389.0 3 425.0 4 369.0 5 335.5 6 347.0 7 338.5 8 341.0 9 351.0 10 348.5 11 343.0 12 364.0 13 406.0 14 473.5 15 576.5 16 669.5 17 830.0 18 1120.5 19 1491.0 20 1983.5 21 2738.5 22 3685.0 23 5008.0 24 6988.0 25 9666.0 26 13373.5 27 16719.5 28 19192.5 29 22459.5 30 28076.0 31 33510.0 32 38147.0 33 43048.0 34 48212.5 35 51782.5 36 56012.5 37 60998.0 38 61403.5 39 61411.5 40 62354.5 41 64033.0 42 65295.0 43 66486.0 44 70236.5 45 73142.5 46 74230.0 47 74295.5 48 72987.0 49 71628.0 50 69441.0 51 64811.0 52 61555.5 53 60357.5 54 57356.5 55 52674.0 56 48164.5 57 44226.0 58 40942.5 59 36401.0 60 30566.5 61 25491.0 62 21264.0 63 16590.5 64 12404.0 65 9803.5 66 7412.5 67 5945.0 68 4654.0 69 3030.0 70 2179.0 71 1788.5 72 1612.0 73 1475.0 74 1107.0 75 877.0 76 634.5 77 383.5 78 280.5 79 175.0 80 91.0 81 64.0 82 47.0 83 33.0 84 22.0 85 16.0 86 16.0 87 10.0 88 6.0 89 7.0 90 5.0 91 3.0 92 2.0 93 1.5 94 1.5 95 1.0 96 0.0 97 0.5 98 1.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.068603549036205E-4 2 2.027441419614482E-4 3 5.068603549036205E-5 4 5.575463903939826E-4 5 0.0013685229582397753 6 8.109765678457928E-4 7 8.616626033361549E-4 8 0.0016219531356915856 9 0.0023822436680470166 10-11 0.002255528579321111 12-13 0.002686359880989189 14-15 0.003826795679522335 16-17 0.003142534200402447 18-19 0.0030665051471669043 20-21 0.003928167750503059 22-23 0.0925527008054011 24-25 0.0029397900584409993 26-27 0.002154156508340387 28-29 0.0021034704728500253 30-31 0.0017993542599078528 32-33 0.0023822436680470166 34-35 0.0012164648517686892 36-37 0.001013720709807241 38-39 0.0014952380469656803 40-41 0.00197675538412412 42-43 0.0024329297035373784 44-45 0.0022301855615759303 46-47 0.0025849878100084643 48-49 3.8014526617771537E-4 50-51 0.001951412366378939 52-53 0.0015966101179464049 54-55 0.002002098401869301 56-57 0.0016979821889271285 58-59 0.0012418078695138701 60-61 0.0013178369227494133 62-63 0.001774011242162672 64-65 0.0012671508872590513 66-67 4.3083130166807746E-4 68-69 3.5480224843253435E-4 70-71 6.335754436295256E-4 72-73 2.5343017745181026E-4 74-75 5.068603549036205E-4 76-77 5.068603549036205E-4 78-79 3.294592306873533E-4 80-81 6.842614791198877E-4 82-83 2.027441419614482E-4 84-85 5.575463903939826E-4 86-87 5.322033726488015E-4 88-89 6.842614791198877E-4 90-91 4.561743194132585E-4 92-93 5.322033726488015E-4 94-95 5.575463903939826E-4 96-97 8.109765678457928E-4 98-99 7.856335501006118E-4 100-101 7.856335501006118E-4 102-103 0.0013685229582397753 104-105 6.842614791198877E-4 106-107 8.363195855909738E-4 108-109 7.856335501006118E-4 110-111 0.0012164648517686892 112-113 8.363195855909738E-4 114-115 5.575463903939826E-4 116-117 0.0013178369227494133 118-119 0.0017486682244174908 120-121 0.0012418078695138701 122-123 0.0011911218340235083 124-125 6.589184613747067E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1972930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.308161267330096 #Duplication Level Percentage of deduplicated Percentage of total 1 47.58084007493741 13.469260940763709 2 14.517849771938485 8.219472651978123 3 10.454914941431346 8.878782545947724 4 7.603212826782143 8.609318994015265 5 5.306390580595178 7.510708015146483 6 3.7818748698883535 6.423475422579752 7 2.4627689148885 4.8801521724781844 8 1.7820483479251927 4.035720961539643 9 1.2130883907072378 3.0906271616099783 >10 4.915905512030053 23.140695832329108 >50 0.2546352446556162 4.969266088425714 >100 0.12231559738867195 5.8911487482203215 >500 0.0036129798538401064 0.6831579948640656 >1k 5.41946978076016E-4 0.19821247010203746 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.013720709807241E-4 0.0 0.0 0.0 0.0 8 1.013720709807241E-4 0.0 0.0 0.0 0.0 9 1.013720709807241E-4 0.0 0.0 0.0 0.0 10-11 1.013720709807241E-4 0.0 0.0 0.0 0.0 12-13 1.013720709807241E-4 0.0 0.0 0.0 0.0 14-15 1.013720709807241E-4 0.0 0.0 5.068603549036205E-5 0.0 16-17 1.013720709807241E-4 0.0 0.0 5.068603549036205E-5 0.0 18-19 1.7740112421626717E-4 0.0 0.0 5.068603549036205E-5 0.0 20-21 2.5343017745181026E-4 0.0 0.0 1.013720709807241E-4 0.0 22-23 3.041162129421723E-4 0.0 0.0 1.013720709807241E-4 0.0 24-25 4.054882839228964E-4 0.0 0.0 1.5205810647108615E-4 0.0 26-27 4.054882839228964E-4 0.0 0.0 3.5480224843253435E-4 0.0 28-29 6.335754436295256E-4 0.0 0.0 6.589184613747067E-4 0.0 30-31 8.616626033361548E-4 0.0 0.0 0.002204842543830749 0.0 32-33 8.616626033361548E-4 0.0 0.0 0.007856335501006118 0.0 34-35 8.616626033361548E-4 0.0 0.0 0.015408554789070062 0.0 36-37 8.616626033361548E-4 0.0 0.0 0.028054720643915395 0.0 38-39 9.123486388265169E-4 0.0 0.0 0.05159838412918857 0.0 40-41 9.123486388265169E-4 0.0 0.0 0.07640919850172079 0.0 42-43 9.123486388265169E-4 0.0 0.0 0.11014075512055674 0.0 44-45 0.001064406745297603 0.0 0.0 0.14992929298049096 0.0 46-47 0.001064406745297603 0.0 0.0 0.1992468055126132 0.0 48-49 0.0012164648517686892 0.0 0.0 0.2533288053808295 0.0 50-51 0.0012671508872590513 0.0 0.0 0.30746149128453615 0.0 52-53 0.0012671508872590513 0.0 0.0 0.3663333215065917 0.0 54-55 0.0012671508872590513 0.0 0.0 0.43787666060123775 0.0 56-57 0.0012671508872590513 0.0 0.0 0.5276162864369238 0.0 58-59 0.0012671508872590513 0.0 0.0 0.6324096648132473 0.0 60-61 0.0012671508872590513 0.0 0.0 0.74199287354341 0.0 62-63 0.0012671508872590513 0.0 0.0 0.856365912627412 0.0 64-65 0.0012671508872590513 0.0 0.0 0.9823713968564521 0.0 66-67 0.0012671508872590513 0.0 0.0 1.1388898744506901 0.0 68-69 0.0012671508872590513 0.0 0.0 1.327720699670034 0.0 70-71 0.0014445520114753185 0.0 0.0 1.5174638735282042 0.0 72-73 0.0015205810647108615 0.0 0.0 1.7089810586285372 0.0 74-75 0.0015205810647108615 0.0 0.0 1.9134485257966576 0.0 76-77 0.0015205810647108615 0.0 0.0 2.1624183321253163 0.0 78-79 0.0015205810647108615 0.0 0.0 2.4668133182626852 0.0 80-81 0.0015205810647108615 0.0 0.0 2.7787605236881188 0.0 82-83 0.0016726391711819476 0.0 0.0 3.0890604329601152 0.0 84-85 0.0016726391711819476 0.0 0.0 3.4204710760138477 0.0 86-87 0.0016726391711819476 0.0 0.0 3.7831803459828786 0.0 88-89 0.0016726391711819476 0.0 0.0 4.199414069429731 0.0 90-91 0.002179499526085568 0.0 0.0 4.648847146122772 0.0 92-93 0.002179499526085568 0.0 0.0 5.097900077549634 0.0 94-95 0.002179499526085568 0.0 0.0 5.58243323381975 0.0 96-97 0.002179499526085568 0.0 0.0 6.089445646829843 0.0 98-99 0.002179499526085568 0.0 0.0 6.661133441125635 0.0 100-101 0.0023569006503018353 0.0 0.0 7.29103414718211 5.068603549036205E-5 102-103 0.0025343017745181025 0.0 0.0 7.902510479337838 5.068603549036205E-5 104-105 0.0025849878100084648 0.0 0.0 8.548453315627011 5.068603549036205E-5 106-107 0.002686359880989189 0.0 0.0 9.244549984033899 5.068603549036205E-5 108-109 0.002686359880989189 0.0 0.0 9.953394190366613 5.068603549036205E-5 110-111 0.002686359880989189 2.5343017745181024E-5 0.0 10.719336215679219 5.068603549036205E-5 112-113 0.002889104022950637 5.068603549036205E-5 0.0 11.464851768689208 5.068603549036205E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3230 0.0 69.25531 1 GTACATG 19145 0.0 56.83634 1 TACATGG 19300 0.0 55.732544 2 ACATGGG 19740 0.0 52.531284 3 CATGGGA 13115 0.0 49.082508 4 GTATCAA 4710 0.0 46.988304 2 CATGGGG 6125 0.0 46.331894 4 ATGGGAG 4995 0.0 41.686966 5 ATGGGGG 4025 0.0 40.795376 5 ATGGGAT 5505 0.0 40.526745 5 TCAACGC 5690 0.0 39.731876 5 ATCAACG 5725 0.0 39.488976 4 CAACGCA 5765 0.0 39.42138 6 AACGCAG 6105 0.0 37.51827 7 TATCAAC 6785 0.0 33.319733 3 AGTACAT 10545 0.0 33.23047 2 GAGTACA 10990 0.0 33.18414 1 TACCTGG 1655 0.0 32.712307 2 CATGGGT 1835 0.0 31.772966 4 ATGGGAA 4910 0.0 31.745895 5 >>END_MODULE