FastQCFastQC Report
Thu 26 May 2016
SRR936216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972930
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.36534494381452964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52040.2637701286918441No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.25718094407809705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.085.3005141
GTATCAA90000.054.63922
GTACATG163250.046.412191
TACATGG168900.044.7652362
ACATGGG169450.043.6361853
CATGGGA112400.041.1548964
ATCAACG125700.040.3525624
TCAACGC126300.040.066595
CAACGCA128500.039.539876
AACGCAG131400.038.6672257
ATGGGAG39350.037.8235325
TATCAAC135850.037.46913
CATGGGG53100.035.9896244
ACGCAGA153100.032.875758
ATGGGAT51050.032.3035935
CGCAGAG155850.032.1047829
GAGTACA108550.030.9490571
AGTACAT107150.030.169942
ATGGGGG34000.029.2418145
GTCGGGA6850.028.6807292
Produced by FastQC (version 0.11.5)