FastQCFastQC Report
Thu 26 May 2016
SRR936215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889319
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.071.005491
GTACATG169350.054.557851
TACATGG174600.052.3721772
ACATGGG171700.051.5588953
CATGGGA121700.049.325514
GTATCAA45000.048.2562
ATGGGAG42750.043.8374635
ATCAACG52050.041.7198874
CAACGCA53200.041.0417066
TCAACGC53400.040.9994055
ATGGGAT51700.040.5063485
CATGGGG46900.039.8316044
AACGCAG55100.039.086617
ATGGGAC25100.035.316985
ATGGGGG29250.035.187735
TATCAAC63850.034.6619533
CGTATAG1301.7382972E-632.035071
GAGTACA98950.031.7460061
AGTACAT95300.031.713332
ACGCAGA67650.030.9560728
Produced by FastQC (version 0.11.5)