##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1889319 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.729226774303335 31.0 30.0 33.0 26.0 34.0 2 29.647154874322442 31.0 30.0 34.0 25.0 34.0 3 29.878680095844057 31.0 30.0 34.0 26.0 34.0 4 33.337437986914864 35.0 35.0 37.0 27.0 37.0 5 33.581195129038555 35.0 35.0 37.0 28.0 37.0 6 33.48181646402752 36.0 35.0 37.0 28.0 37.0 7 33.430197335653745 36.0 35.0 37.0 28.0 37.0 8 33.397928565795404 37.0 35.0 37.0 27.0 37.0 9 34.76871190095479 39.0 35.0 39.0 27.0 39.0 10-11 34.62720641670359 39.0 35.0 39.0 27.0 39.0 12-13 34.62597634385723 39.0 35.0 39.0 27.0 39.0 14-15 35.725698518884315 39.0 36.0 41.0 27.0 41.0 16-17 35.74249345928348 39.0 36.0 41.0 27.0 41.0 18-19 35.77548788743457 39.5 36.0 41.0 27.0 41.0 20-21 35.90902674455717 40.0 36.0 41.0 27.0 41.0 22-23 35.79110885985903 39.0 35.5 41.0 27.0 41.0 24-25 35.7716664046675 39.0 35.0 41.0 27.0 41.0 26-27 35.88446233801703 39.0 35.0 41.0 27.0 41.0 28-29 35.7766470352545 39.0 35.0 41.0 27.0 41.0 30-31 35.69878988143347 39.0 35.0 41.0 27.0 41.0 32-33 35.55191950115359 39.0 35.0 41.0 27.0 41.0 34-35 35.51003112761794 39.0 35.0 41.0 27.0 41.0 36-37 35.39288283238564 39.0 35.0 41.0 27.0 41.0 38-39 35.15480736709894 39.0 34.0 40.5 25.5 41.0 40-41 35.251646757376605 39.0 34.5 40.5 26.5 41.0 42-43 35.15980228854947 39.0 34.0 41.0 26.0 41.0 44-45 35.00746221257501 39.0 34.0 40.0 25.5 41.0 46-47 34.98380924555356 39.0 34.0 40.0 26.0 41.0 48-49 34.89976732357003 38.5 34.0 40.0 26.0 41.0 50-51 34.76644944554096 38.0 33.5 40.0 25.5 41.0 52-53 34.62876491476558 38.0 33.0 40.0 24.5 41.0 54-55 34.47041844177717 38.0 33.0 40.0 24.0 41.0 56-57 34.28066462042673 38.0 33.0 40.0 24.0 41.0 58-59 34.05541441122436 38.0 33.0 40.0 23.0 41.0 60-61 33.819378040447376 37.5 33.0 40.0 23.0 41.0 62-63 33.57510483936275 37.0 32.0 40.0 22.5 41.0 64-65 33.29986915920499 37.0 32.0 40.0 22.0 41.0 66-67 32.995879732326834 36.0 32.0 39.0 21.5 41.0 68-69 32.714847519132555 36.0 31.5 39.0 20.5 41.0 70-71 32.37707528479839 35.5 31.0 39.0 20.0 40.0 72-73 32.04977534233234 35.0 31.0 38.5 18.5 40.0 74-75 31.713005320964854 35.0 31.0 37.5 18.0 40.0 76-77 31.395196364404317 35.0 31.0 37.0 18.0 39.0 78-79 31.05574601218746 35.0 30.5 36.5 16.0 39.0 80-81 30.735664014388252 34.5 30.0 36.0 12.5 38.5 82-83 30.431325255290396 34.0 30.0 36.0 10.5 37.0 84-85 30.45072854293002 34.0 30.5 35.0 11.0 37.0 86-87 30.322266118109226 34.0 31.0 35.0 9.0 37.0 88-89 30.143083036797915 34.0 31.0 35.0 7.0 36.0 90-91 29.968482823705262 34.0 30.0 35.0 7.0 36.0 92-93 29.83966577375234 34.0 30.0 35.0 3.5 36.0 94-95 29.703892513651745 34.0 30.0 35.0 2.0 35.5 96-97 29.591327615929337 34.0 30.0 35.0 2.0 35.0 98-99 29.486141038120085 34.0 30.0 35.0 2.0 35.0 100-101 29.38258467733612 34.0 30.0 35.0 2.0 35.0 102-103 28.387376086304116 33.5 28.0 34.5 2.0 35.0 104-105 29.061713506295128 34.0 29.5 35.0 2.0 35.0 106-107 29.186323220165576 34.0 30.0 35.0 2.0 35.0 108-109 29.14199693116938 34.0 30.0 35.0 2.0 35.0 110-111 29.03164500012968 34.0 30.0 35.0 2.0 35.0 112-113 28.98912862253542 34.0 30.0 35.0 2.0 35.0 114-115 28.872183575140035 34.0 29.5 35.0 2.0 35.0 116-117 28.744471156009123 34.0 29.0 35.0 2.0 35.0 118-119 28.53371399959456 34.0 29.0 35.0 2.0 35.0 120-121 28.326827550032576 34.0 29.0 35.0 2.0 35.0 122-123 28.023298871180568 34.0 29.0 35.0 2.0 35.0 124-125 25.992158020958875 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3815790415522642 1101 2 0.4490699399873783 1101 3 0.32584960261727147 1101 4 0.4974717146002874 1101 5 0.43243919916825746 1101 6 0.4522166411667925 1101 7 0.244651008835298 1101 8 0.41179383658868574 1101 9 0.5526232781127831 1101 10-11 0.6383463313251738 1101 12-13 0.6134698817514703 1101 14-15 0.6027629434982984 1101 16-17 0.556182919810233 1101 18-19 0.5634423394127879 1101 20-21 0.4529681414520752 1101 22-23 0.5799368415619028 1101 24-25 0.6291802857063189 1101 26-27 0.564661496464943 1101 28-29 0.5937873890984733 1101 30-31 0.6655202339039974 1101 32-33 0.5789207209404168 1101 34-35 0.6502136268910021 1101 36-37 0.5343294527232558 1101 38-39 0.6614451775874883 1101 40-41 0.5831027537062567 1101 42-43 0.7224063446934252 1101 44-45 0.7513041655634893 1101 46-47 0.647685895706914 1101 48-49 0.6309724060872455 1101 50-51 0.5934890640329087 1101 52-53 0.5659809460073362 1101 54-55 0.6341493673375211 1101 56-57 0.6970003065461583 1101 58-59 0.8052615084948513 1101 60-61 0.7573663929942995 1101 62-63 0.6668636809814288 1101 64-65 0.6764275655878365 1101 66-67 0.7140242159708521 1101 68-69 0.6943980281844375 1101 70-71 0.8406691357224503 1101 72-73 0.6964726603821063 1101 74-75 0.6584611668031677 1101 76-77 0.6913581527542227 1101 78-79 0.6760049638878627 1101 80-81 0.6010234326455972 1101 82-83 0.6330204336618195 1101 84-85 0.4485739088729943 1101 86-87 0.5134325670253759 1101 88-89 0.47055943468596695 1101 90-91 0.4949738605338041 1101 92-93 0.5435177420996524 1101 94-95 0.5592139274978898 1101 96-97 0.5789913849970745 1101 98-99 0.6107306728374198 1101 100-101 0.5618408690089431 1101 102-103 0.5812259596008715 1101 104-105 0.5820623572350563 1101 106-107 0.546522315654201 1101 108-109 0.5650229380576732 1101 110-111 0.5787564833331977 1101 112-113 0.5308993303827059 1101 114-115 0.6094306498943709 1101 116-117 0.5194730435380599 1101 118-119 0.5334747179089057 1101 120-121 0.5289507175884332 1101 122-123 0.4524107899808385 1101 124-125 0.4007801041530996 1102 1 0.3208166996534132 1102 2 0.173830416442172 1102 3 0.41574240958386 1102 4 0.43037700716666194 1102 5 0.42429188141540664 1102 6 0.29889047384990874 1102 7 0.3713498441606333 1102 8 0.36222714266025235 1102 9 0.3747693214056369 1102 10-11 0.6055744864026096 1102 12-13 0.4905341230960474 1102 14-15 0.6816761272510874 1102 16-17 0.5845544006866206 1102 18-19 0.5646648731416235 1102 20-21 0.5248587132366183 1102 22-23 0.5923344248846334 1102 24-25 0.6389475883139113 1102 26-27 0.4749782880205018 1102 28-29 0.48174260604757535 1102 30-31 0.4638899555986953 1102 32-33 0.573459110174916 1102 34-35 0.5501861891649398 1102 36-37 0.5069793414862858 1102 38-39 0.5763888579539369 1102 40-41 0.5417275578925782 1102 42-43 0.701339369445094 1102 44-45 0.6570330273545792 1102 46-47 0.4843163845861511 1102 48-49 0.42297344254706104 1102 50-51 0.42167777591601663 1102 52-53 0.5533014116476949 1102 54-55 0.4380861588953735 1102 56-57 0.3529110824118362 1102 58-59 0.44752594597729 1102 60-61 0.5085977341069636 1102 62-63 0.5572659735864747 1102 64-65 0.5725315920942151 1102 66-67 0.4687822975057685 1102 68-69 0.4270557156419983 1102 70-71 0.4357916926201568 1102 72-73 0.29522873647285053 1102 74-75 0.3820078882244111 1102 76-77 0.4339911347829144 1102 78-79 0.4057411245042495 1102 80-81 0.2759768051506555 1102 82-83 0.24953965458646366 1102 84-85 0.2735322903983075 1102 86-87 0.27267512742538 1102 88-89 0.3948287861721873 1102 90-91 0.36037328141532754 1102 92-93 0.282219073558057 1102 94-95 0.2930690748944791 1102 96-97 0.2703173111006585 1102 98-99 0.27517720937819234 1102 100-101 0.334506743030186 1102 102-103 0.3568759588721804 1102 104-105 0.33775792309885233 1102 106-107 0.32965790266030837 1102 108-109 0.48211108728952823 1102 110-111 0.45240971385289797 1102 112-113 0.44954360376435787 1102 114-115 0.32978163934513205 1102 116-117 0.37720992572877066 1102 118-119 0.46146101488195157 1102 120-121 0.391423046050722 1102 122-123 0.3339242806551681 1102 124-125 0.3130322824457643 1103 1 0.23094242697525047 1103 2 0.3249815396738356 1103 3 0.26881865286500073 1103 4 0.3653706719613865 1103 5 0.20903354993765078 1103 6 0.13395929542765828 1103 7 0.22519455223891072 1103 8 0.2019771184721293 1103 9 0.3470165161619292 1103 10-11 0.37284613778252407 1103 12-13 0.295434683288363 1103 14-15 0.28135874203125155 1103 16-17 0.2640706706140463 1103 18-19 0.39538538758714026 1103 20-21 0.3137811658799734 1103 22-23 0.43851091902786976 1103 24-25 0.485067257725909 1103 26-27 0.34689511235361437 1103 28-29 0.4011748587002799 1103 30-31 0.4127026774871183 1103 32-33 0.45776164272935205 1103 34-35 0.4223991154835218 1103 36-37 0.36763370083431113 1103 38-39 0.37174448355603573 1103 40-41 0.415186056858623 1103 42-43 0.43524367752355886 1103 44-45 0.526698909508184 1103 46-47 0.34349937688622845 1103 48-49 0.27986996274403 1103 50-51 0.22937935557411038 1103 52-53 0.16082526067491187 1103 54-55 0.24968630860712437 1103 56-57 0.13720243519227182 1103 58-59 0.23419508392181854 1103 60-61 0.23841922575269336 1103 62-63 0.34457135177532194 1103 64-65 0.41476195006456607 1103 66-67 0.4051668372902526 1103 68-69 0.3409210542224983 1103 70-71 0.36882924416169516 1103 72-73 0.13630396686183843 1103 74-75 0.1810500037987417 1103 76-77 0.17527646622502147 1103 78-79 0.10355652027982032 1103 80-81 0.18022499196722563 1103 82-83 0.2525481826939888 1103 84-85 0.2678545623135591 1103 86-87 0.3166281890400562 1103 88-89 0.36092589003206044 1103 90-91 0.2408231897782116 1103 92-93 0.26491339677897585 1103 94-95 0.3489232721242068 1103 96-97 0.4157389158866174 1103 98-99 0.2348984279411539 1103 100-101 0.30881079631165065 1103 102-103 0.27813979644649933 1103 104-105 0.29321074792452606 1103 106-107 0.2824539109023263 1103 108-109 0.2910280333733901 1103 110-111 0.17549597032293462 1103 112-113 0.3436079538954324 1103 114-115 0.3360459005009524 1103 116-117 0.2555325522488623 1103 118-119 0.2357902168682955 1103 120-121 0.2910635688660257 1103 122-123 0.317793000943702 1103 124-125 0.3654860808144882 1104 1 0.0700776602704245 1104 2 0.09003956629329579 1104 3 0.1408066817886997 1104 4 0.09923641888266843 1104 5 0.07252880795768846 1104 6 -0.057065871995504835 1104 7 -0.21688718245160743 1104 8 -0.030030149028206665 1104 9 0.0553934349904992 1104 10-11 0.06443959300894875 1104 12-13 0.06656557144494002 1104 14-15 0.09564018485135506 1104 16-17 0.07660884602159257 1104 18-19 -0.02029679551582575 1104 20-21 -0.04136030715127248 1104 22-23 0.09818539024131923 1104 24-25 0.06677497400445276 1104 26-27 -0.06280153259954346 1104 28-29 -0.08245954982320569 1104 30-31 -0.0666842026082648 1104 32-33 0.08983140817232993 1104 34-35 0.026121240578156346 1104 36-37 0.07503726106824615 1104 38-39 0.1818321178328901 1104 40-41 0.039275104768528024 1104 42-43 0.1352005187394667 1104 44-45 0.1804616788391229 1104 46-47 0.12612586321256458 1104 48-49 0.10665167987843205 1104 50-51 0.08659738789930316 1104 52-53 0.05595741616357941 1104 54-55 0.056436680760207025 1104 56-57 -0.0635782633216806 1104 58-59 0.07929505319861363 1104 60-61 0.059134939207623916 1104 62-63 0.06400107976525504 1104 64-65 0.18307631608762875 1104 66-67 0.06144895492354863 1104 68-69 -0.039292974942597425 1104 70-71 0.020317033517571303 1104 72-73 0.143023524374577 1104 74-75 0.18463780632170312 1104 76-77 0.07257250439494456 1104 78-79 -0.009145055015796544 1104 80-81 0.03673506478852673 1104 82-83 0.06699101376392491 1104 84-85 -0.0019493303893156622 1104 86-87 0.08395296620540194 1104 88-89 0.07286546590556853 1104 90-91 0.17419750262087774 1104 92-93 0.16907986532975983 1104 94-95 0.11472263315475217 1104 96-97 3.3187472411100316E-5 1104 98-99 0.07933501378873231 1104 100-101 0.06706034186428411 1104 102-103 0.045964740029191375 1104 104-105 -0.02460454893841657 1104 106-107 0.08836831020885683 1104 108-109 0.014426860771170169 1104 110-111 0.03342424810457345 1104 112-113 -5.385445738887995E-4 1104 114-115 0.06307984963941848 1104 116-117 -0.008870320370547802 1104 118-119 -0.02491809924419286 1104 120-121 0.06492388436404894 1104 122-123 0.16029795688882587 1104 124-125 0.2360014991072319 1105 1 0.266757479775503 1105 2 0.4004254038856665 1105 3 0.3482695339072386 1105 4 0.37622358642813936 1105 5 0.307572572756321 1105 6 0.24136564189934262 1105 7 0.24799467149113497 1105 8 0.21023650305919261 1105 9 0.2752477638177595 1105 10-11 0.3863063781096585 1105 12-13 0.2707849867083496 1105 14-15 0.33229441864339293 1105 16-17 0.25633610574042365 1105 18-19 0.195424153720424 1105 20-21 0.1357827325538281 1105 22-23 0.15319011725355125 1105 24-25 0.3095007523978026 1105 26-27 0.10301400187906751 1105 28-29 0.2823510502036086 1105 30-31 0.3005486818276424 1105 32-33 0.47956181098307127 1105 34-35 0.48281446265908556 1105 36-37 0.4450114638658462 1105 38-39 0.5123193443253982 1105 40-41 0.4143387203186748 1105 42-43 0.6334454945171828 1105 44-45 0.6510012107006702 1105 46-47 0.4664807558614683 1105 48-49 0.4248429936053526 1105 50-51 0.4890987670799305 1105 52-53 0.41740977885973507 1105 54-55 0.4800561164525874 1105 56-57 0.345428663629896 1105 58-59 0.5542855924244918 1105 60-61 0.4695740878684447 1105 62-63 0.46832919831089015 1105 64-65 0.4652826023614267 1105 66-67 0.5260757279468606 1105 68-69 0.5851288603890836 1105 70-71 0.4046108660377925 1105 72-73 0.4776156131425111 1105 74-75 0.5561641497966257 1105 76-77 0.36484365940584595 1105 78-79 0.29837105192402547 1105 80-81 0.42015982227322723 1105 82-83 0.3665415088740218 1105 84-85 0.26024387504245183 1105 86-87 0.41689909401517156 1105 88-89 0.3747863855333087 1105 90-91 0.39375512762234166 1105 92-93 0.42935318608172324 1105 94-95 0.4288760749866256 1105 96-97 0.4465836947318955 1105 98-99 0.48036052345559455 1105 100-101 0.3978925885022768 1105 102-103 0.48805737776951474 1105 104-105 0.44115042983614927 1105 106-107 0.5565319615771926 1105 108-109 0.5854494883330013 1105 110-111 0.5227164179584278 1105 112-113 0.4271939654808534 1105 114-115 0.451618093665747 1105 116-117 0.4835712973160824 1105 118-119 0.5314249167021039 1105 120-121 0.4784125858823636 1105 122-123 0.49420180527525304 1105 124-125 0.5978275117587941 1106 1 -0.4327588008555807 1106 2 -0.5321758045735479 1106 3 -0.27256061043649993 1106 4 -0.30505133804315676 1106 5 -0.3012129257331111 1106 6 -0.47689518388111907 1106 7 -0.5111624282067453 1106 8 -0.37290146270112956 1106 9 -0.39381568029099867 1106 10-11 -0.28595763666039886 1106 12-13 -0.2831953758294077 1106 14-15 -0.5244523084462358 1106 16-17 -0.45422582779431764 1106 18-19 -0.5740050171422837 1106 20-21 -0.5291833634515442 1106 22-23 -0.6132976303127435 1106 24-25 -4.5659104594182445 1106 26-27 -1.4355303082921296 1106 28-29 -1.067615045801766 1106 30-31 -1.0392034161918247 1106 32-33 -5.816209253132403 1106 34-35 -1.5605338085728704 1106 36-37 -0.7694260990747352 1106 38-39 -0.4807907597363723 1106 40-41 -0.9527937066702492 1106 42-43 -0.6619893829584811 1106 44-45 -0.7276580709374656 1106 46-47 -0.7633191770019039 1106 48-49 -0.5982330386202293 1106 50-51 -0.676724327918393 1106 52-53 -0.5120269448730568 1106 54-55 -0.7306897714292475 1106 56-57 -0.533405341069674 1106 58-59 -0.4410682175788665 1106 60-61 -0.40250983291034004 1106 62-63 -0.44135861639203 1106 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-1.278418895976067 2216 108-109 -1.2993596239580185 2216 110-111 -1.3898405840456363 2216 112-113 -1.4183188657552996 2216 114-115 -1.4456139146672236 2216 116-117 -1.3009349752742168 2216 118-119 -1.4784723821082828 2216 120-121 -1.4991195633036902 2216 122-123 -1.389581689152518 2216 124-125 -1.1997375031462063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47621.0 3 8537.0 4 5761.0 5 4839.0 6 5351.0 7 5783.0 8 6065.0 9 6883.0 10 8012.0 11 9286.0 12 10244.0 13 10605.0 14 10468.0 15 9846.0 16 8800.0 17 8298.0 18 7195.0 19 6984.0 20 6753.0 21 7062.0 22 8442.0 23 11576.0 24 16720.0 25 24124.0 26 28258.0 27 27866.0 28 32479.0 29 39720.0 30 46806.0 31 57672.0 32 74009.0 33 97374.0 34 133733.0 35 192994.0 36 313904.0 37 438009.0 38 150809.0 39 431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.056422715171145 18.304989652306926 9.410419677024946 22.22816795549698 2 18.777516543006524 21.112115588799767 37.80337666819455 22.306991199999153 3 17.146716434184185 29.300678869306278 30.496507205245493 23.056097491264044 4 10.771651676193066 16.35935004877439 37.40479995088156 35.46419832415098 5 10.753289179437262 43.45898297836078 34.481641623012955 11.306086219189002 6 29.893680705485444 37.82880407598757 19.272178346686538 13.005336871840445 7 27.583332274742446 35.24832425062351 21.683699394062575 15.484644080571469 8 27.27359820800976 34.55096501554018 19.94308991285643 18.232346863593634 9 28.00554496070732 13.822069007131276 18.970106648924485 39.20227938323692 10-11 23.831826281325018 27.643030999301587 29.19864636585967 19.326496353513726 12-13 24.52436806480366 26.690938751140646 28.65989405465374 20.124799129401953 14-15 22.512225066673157 28.084478869564087 25.503221245851865 23.900074817910884 16-17 22.28331304765058 30.238079547929658 26.088023189989112 21.39058421443065 18-19 21.523540383980215 29.16159414459811 27.936910881697845 21.37795458972383 20-21 22.997949981235948 28.37624540095519 27.446538337150546 21.17926628065831 22-23 22.713520025556765 28.749398033337165 27.056765787420073 21.480316153685997 24-25 22.10069971451944 28.549273383562596 27.517314222606025 21.832712679311935 26-27 22.07414135415393 28.61704678441504 27.402406097974737 21.906405763456295 28-29 21.93170450274971 28.644808367164387 27.475162627230336 21.94832450285557 30-31 22.230640998192182 28.25524117323097 27.68640480009612 21.827713028480723 32-33 21.82989861243804 28.724517476188083 27.588452774462297 21.857131136911583 34-35 22.149096806429487 28.75192664357517 27.52861383287743 21.57036271711791 36-37 22.204287928252946 28.59423364371218 27.27208008875226 21.929398339282614 38-39 21.97962470177407 28.61833565121427 27.44588927895157 21.956150368060083 40-41 22.06129761410434 28.45298274669777 27.77550620193136 21.71021343726653 42-43 21.885946572521952 28.383096295433734 27.638133902153715 22.092823229890595 44-45 21.85339466148659 28.548793462094224 27.638769061393432 21.95904281502575 46-47 21.971392336991155 28.592022162893453 27.23367688499982 22.202908615115568 48-49 21.879890171689436 28.428806774951205 27.584557874888354 22.106745178471005 50-51 21.949674216239963 28.538048684955726 27.54410386970767 21.968173229096642 52-53 22.055557834480368 28.256900400546765 27.653050017400695 22.034491747572176 54-55 21.765740349892884 28.398090819711612 27.653711640733235 22.18245718966227 56-57 22.032157002645963 28.463398742280845 27.43565979443104 22.068784460642156 58-59 22.162755831847996 28.283056842406122 27.393892719667708 22.160294606078168 60-61 22.177440804149473 28.37547547359459 27.73979046742432 21.707293254831615 62-63 21.803329709404675 28.34003072047019 27.85556403608967 22.001075534035465 64-65 21.7902777696913 28.28607330020288 27.63956366885284 22.284085261252983 66-67 21.869339876801845 28.761841599649397 27.324944397807883 22.043874125740878 68-69 21.965765369988592 28.397461513829086 27.52320285394442 22.1135702622379 70-71 21.78555417409664 28.262055875514143 27.72857984435563 22.223810106033586 72-73 21.91271287791114 28.53963324832023 27.457072438630586 22.090581435138052 74-75 21.540821373723375 28.710050571359236 27.63142956120765 22.117698493709742 76-77 21.6489203195454 28.64518191571627 27.57489643038804 22.131001334350284 78-79 21.853863850546773 28.5715419913106 27.39238352978476 22.182210628357865 80-81 21.73129572607953 28.67185463456849 27.287220317497287 22.3096293218547 82-83 21.964236234888368 28.688897481070512 27.33705796467449 22.009808319366627 84-85 21.75940469256096 28.530635134158803 27.408320341373393 22.30163983190684 86-87 21.53850388197381 28.550139614499372 27.62003790273642 22.291318600790394 88-89 21.671559110531476 28.447452718138074 27.607991920851443 22.27299625047901 90-91 21.833170928449576 28.495123354492723 27.5381342722294 22.133571444828302 92-93 21.873856230771775 28.429388997320455 27.476238049555064 22.220516722352706 94-95 21.65205242865056 28.328879768571657 27.604462051102768 22.414605751675015 96-97 21.695562608108364 28.460711540945148 27.610056168713626 22.233669682232858 98-99 21.50281068741361 28.503395293458205 27.40659072522113 22.58720329390705 100-101 21.456654258249973 28.565920132704605 27.47816794394141 22.499257665104015 102-103 21.647241026232752 28.34335071985713 27.520082189235843 22.48932606467427 104-105 21.44652962883765 28.2924224107451 27.732402551622076 22.528645408795178 106-107 21.44829332879551 28.451445014347858 27.63085290819674 22.46940874865989 108-109 21.533056211955245 28.75661387563519 27.210992193158912 22.499337719250654 110-111 21.421149938522234 28.85310493140055 27.241397744456812 22.484347385620396 112-113 21.65852346918738 28.54313777485607 27.385041181766645 22.4132975741899 114-115 21.38752845617101 28.756968817700017 27.278835807165475 22.576666918963497 116-117 21.474667476137334 28.76064448191275 27.29258791812102 22.4721001238289 118-119 21.49793427525759 28.68958340666341 27.16133613262347 22.65114618545553 120-121 21.58204096755412 28.64455089186471 27.232281797491137 22.541126343090035 122-123 21.58513525494197 28.71461822550915 27.14335052221653 22.556895997332347 124-125 21.771149255548323 28.615473373877236 26.814657202894175 22.798720167680266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 313.0 1 414.0 2 560.0 3 598.0 4 542.5 5 474.0 6 438.0 7 428.5 8 417.5 9 414.5 10 438.0 11 425.0 12 425.5 13 462.0 14 535.5 15 625.0 16 724.5 17 902.0 18 1129.5 19 1410.0 20 1950.5 21 2907.0 22 4107.5 23 5869.5 24 8292.0 25 10913.0 26 14320.5 27 17725.0 28 20338.0 29 23831.5 30 28989.0 31 33804.5 32 37424.0 33 42267.5 34 47699.5 35 50857.5 36 54680.0 37 60287.0 38 62205.5 39 61914.0 40 61359.5 41 62207.0 42 63360.5 43 63991.5 44 66424.5 45 67637.5 46 67030.0 47 66210.0 48 66646.0 49 66328.0 50 65190.0 51 62556.0 52 58686.0 53 56555.5 54 54149.5 55 49857.5 56 45162.0 57 40863.5 58 37005.5 59 32792.0 60 28136.0 61 23048.5 62 18165.5 63 14189.5 64 11034.0 65 8927.0 66 6901.0 67 5301.5 68 4167.0 69 3074.5 70 2311.5 71 1820.5 72 1470.5 73 1220.5 74 1041.0 75 861.0 76 563.5 77 361.5 78 271.5 79 180.5 80 103.5 81 62.0 82 40.0 83 34.0 84 26.0 85 17.0 86 14.0 87 8.0 88 3.5 89 6.0 90 6.0 91 5.0 92 5.5 93 4.5 94 3.5 95 2.5 96 3.0 97 3.5 98 2.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.763621177789457E-4 2 5.292912419766064E-5 3 5.292912419766064E-5 4 4.234329935812851E-4 5 9.527242355578914E-4 6 5.292912419766064E-4 7 7.939368629649095E-4 8 0.0012173698565461947 9 0.0019054484711157828 10-11 0.001826054784819292 12-13 0.0022759523404994072 14-15 0.0034668576349467716 16-17 0.0029375663929701655 18-19 0.002911101830871335 20-21 0.0035991804454409237 22-23 0.09294354209109208 24-25 0.002567062523586541 26-27 0.0019054484711157828 28-29 0.0015349446017321586 30-31 0.001243834418645025 32-33 0.0017995902227204619 34-35 0.0012173698565461947 36-37 7.939368629649095E-4 38-39 0.0011379761702497036 40-41 0.0015084800396333283 42-43 0.0015349446017321586 44-45 0.0015349446017321586 46-47 0.0018525193469181224 48-49 3.4403930728479416E-4 50-51 0.0015349446017321586 52-53 0.001402621791238007 54-55 0.001402621791238007 56-57 0.0013761572291391766 58-59 8.733305492614004E-4 60-61 9.79188797656722E-4 62-63 0.001323228104941516 64-65 0.0010585824839532128 66-67 2.646456209883032E-4 68-69 2.1171649679064256E-4 70-71 6.351494903719276E-4 72-73 1.323228104941516E-4 74-75 4.4989755568011547E-4 76-77 5.292912419766064E-4 78-79 2.9111018308713353E-4 80-81 6.086849282730973E-4 82-83 2.3818105888947285E-4 84-85 5.028266798777761E-4 86-87 5.028266798777761E-4 88-89 5.822203661742671E-4 90-91 4.234329935812851E-4 92-93 3.4403930728479416E-4 94-95 4.4989755568011547E-4 96-97 5.822203661742671E-4 98-99 6.61614052470758E-4 100-101 5.292912419766064E-4 102-103 0.001085047046052043 104-105 5.822203661742671E-4 106-107 6.61614052470758E-4 108-109 7.674723008660793E-4 110-111 9.527242355578914E-4 112-113 6.351494903719276E-4 114-115 5.292912419766064E-4 116-117 9.79188797656722E-4 118-119 0.0016143382880286494 120-121 0.0010056533597555522 122-123 8.733305492614004E-4 124-125 4.763621177789457E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1889319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.7613244185426 #Duplication Level Percentage of deduplicated Percentage of total 1 49.42606858928394 15.204113306081018 2 14.756757069549572 9.07874783164073 3 10.476134955300584 9.667793580373067 4 7.331137901104248 9.020620453317651 5 5.067762817714439 7.7945548055970715 6 3.373198438183442 6.225843089104925 7 2.28239684444739 4.914668484873285 8 1.5509104823490607 3.816644839332627 9 1.096529218881478 3.035762193278183 >10 4.341749796238017 21.942333739921242 >50 0.20581959057357338 4.3267148863067355 >100 0.08997129043816936 4.659575083130334 >500 0.0015630059358939514 0.3126277070431699 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.292912419766064E-5 0.0 14-15 0.0 0.0 0.0 1.323228104941516E-4 0.0 16-17 0.0 0.0 0.0 2.1171649679064256E-4 0.0 18-19 0.0 0.0 0.0 2.1171649679064256E-4 0.0 20-21 0.0 0.0 0.0 2.1171649679064256E-4 0.0 22-23 0.0 0.0 0.0 2.646456209883032E-4 0.0 24-25 0.0 0.0 0.0 3.969684314824548E-4 0.0 26-27 0.0 0.0 0.0 4.234329935812851E-4 0.0 28-29 0.0 0.0 0.0 5.292912419766064E-4 0.0 30-31 0.0 0.0 0.0 0.00248766883729005 0.0 32-33 0.0 0.0 0.0 0.007859974943352605 0.0 34-35 0.0 0.0 0.0 0.014608438278554337 0.0 36-37 0.0 0.0 0.0 0.02842293969414376 0.0 38-39 0.0 0.0 0.0 0.05322023438074777 0.0 40-41 0.0 0.0 0.0 0.07844096206093307 0.0 42-43 0.0 0.0 0.0 0.10890167303668677 0.0 44-45 0.0 0.0 0.0 0.14788397300826384 0.0 46-47 0.0 0.0 0.0 0.19729331044678003 0.0 48-49 0.0 0.0 0.0 0.24993132446135352 0.0 50-51 0.0 0.0 0.0 0.30344266902518846 0.0 52-53 0.0 0.0 0.0 0.3637024769242251 0.0 54-55 0.0 0.0 0.0 0.4375650697420605 0.0 56-57 0.0 0.0 0.0 0.5271740770086999 0.0 58-59 2.646456209883032E-5 0.0 0.0 0.6294331449585804 0.0 60-61 5.292912419766064E-5 0.0 0.0 0.7328831182029081 0.0 62-63 5.292912419766064E-5 0.0 0.0 0.8421023659847808 0.0 64-65 5.292912419766064E-5 0.0 0.0 0.9670150990912598 0.0 66-67 5.292912419766064E-5 0.0 0.0 1.1235000547816436 0.0 68-69 5.292912419766064E-5 0.0 0.0 1.3059732104530786 0.0 70-71 5.292912419766064E-5 0.0 0.0 1.492918877119216 0.0 72-73 5.292912419766064E-5 0.0 0.0 1.679467575353871 0.0 74-75 5.292912419766064E-5 0.0 0.0 1.890178418784758 0.0 76-77 5.292912419766064E-5 0.0 0.0 2.137516216160426 0.0 78-79 5.292912419766064E-5 0.0 0.0 2.427991249757188 0.0 80-81 5.292912419766064E-5 0.0 0.0 2.724447274388285 0.0 82-83 5.292912419766064E-5 0.0 0.0 3.0351676979906514 0.0 84-85 5.292912419766064E-5 0.0 0.0 3.3690975425536926 0.0 86-87 5.292912419766064E-5 0.0 0.0 3.7331440587852027 0.0 88-89 5.292912419766064E-5 0.0 0.0 4.137257922034342 0.0 90-91 5.292912419766064E-5 0.0 0.0 4.582656502157656 0.0 92-93 5.292912419766064E-5 0.0 0.0 5.023953075155651 0.0 94-95 5.292912419766064E-5 0.0 0.0 5.501691350163736 0.0 96-97 5.292912419766064E-5 0.0 0.0 6.017935563025619 0.0 98-99 5.292912419766064E-5 0.0 0.0 6.576179035938346 0.0 100-101 5.292912419766064E-5 0.0 0.0 7.17655409171241 0.0 102-103 5.292912419766064E-5 0.0 0.0 7.777908336284132 0.0 104-105 5.292912419766064E-5 0.0 0.0 8.400910592652696 0.0 106-107 5.292912419766064E-5 0.0 0.0 9.081129232278933 0.0 108-109 5.292912419766064E-5 0.0 0.0 9.796201700189329 0.0 110-111 5.292912419766064E-5 0.0 0.0 10.536309643845215 0.0 112-113 5.292912419766064E-5 0.0 0.0 11.295233891153373 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3075 0.0 71.00549 1 GTACATG 16935 0.0 54.55785 1 TACATGG 17460 0.0 52.372177 2 ACATGGG 17170 0.0 51.558895 3 CATGGGA 12170 0.0 49.32551 4 GTATCAA 4500 0.0 48.256 2 ATGGGAG 4275 0.0 43.837463 5 ATCAACG 5205 0.0 41.719887 4 CAACGCA 5320 0.0 41.041706 6 TCAACGC 5340 0.0 40.999405 5 ATGGGAT 5170 0.0 40.506348 5 CATGGGG 4690 0.0 39.831604 4 AACGCAG 5510 0.0 39.08661 7 ATGGGAC 2510 0.0 35.31698 5 ATGGGGG 2925 0.0 35.18773 5 TATCAAC 6385 0.0 34.661953 3 CGTATAG 130 1.7382972E-6 32.03507 1 GAGTACA 9895 0.0 31.746006 1 AGTACAT 9530 0.0 31.71333 2 ACGCAGA 6765 0.0 30.956072 8 >>END_MODULE