FastQCFastQC Report
Thu 26 May 2016
SRR936215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889319
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70980.3756909235549952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.25919392119594414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47720.25257778067123654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.087.816391
GTATCAA80200.055.5958182
GTACATG145250.048.7281721
ACATGGG145500.047.5011253
TACATGG148450.047.39932
CATGGGA100250.044.773564
ATCAACG109850.042.107134
TCAACGC111200.041.5959365
CAACGCA112200.041.199026
AACGCAG113850.040.549687
TATCAAC120450.038.450983
ATGGGAG37900.037.696995
ATGGGAT40900.036.9696275
CATGGGG42750.035.369774
ACGCAGA134750.033.951298
CGCAGAG136100.033.395969
AGTACAT89200.030.4990272
GAGTACA93300.030.2674981
ATGGGGG26800.030.2091675
CATGGGC20550.028.6786044
Produced by FastQC (version 0.11.5)