##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936215_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1889319 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50735106141419 31.0 31.0 34.0 30.0 34.0 2 31.438698811582373 31.0 31.0 34.0 30.0 34.0 3 31.800911333660434 31.0 31.0 34.0 30.0 34.0 4 35.61877533651014 37.0 35.0 37.0 33.0 37.0 5 35.23082020558731 37.0 35.0 37.0 33.0 37.0 6 35.41772988044899 37.0 35.0 37.0 33.0 37.0 7 35.249837110620284 37.0 35.0 37.0 32.0 37.0 8 35.328411983365434 37.0 35.0 37.0 32.0 37.0 9 37.01572630138161 39.0 37.0 39.0 33.0 39.0 10-11 36.89937300159475 39.0 37.0 39.0 33.0 39.0 12-13 36.836569949278015 39.0 37.0 39.0 32.0 39.0 14-15 37.981440137954465 40.0 37.0 41.0 33.0 41.0 16-17 37.89440136895887 40.0 37.0 41.0 32.5 41.0 18-19 38.011107441358504 40.0 37.0 41.0 33.0 41.0 20-21 38.07348970713787 40.0 37.0 41.0 33.0 41.0 22-23 37.98339772161292 40.0 37.0 41.0 33.0 41.0 24-25 37.94540678413756 40.0 37.0 41.0 33.0 41.0 26-27 37.77933583476374 40.0 37.0 41.0 32.0 41.0 28-29 37.62988965865479 40.0 37.0 41.0 32.0 41.0 30-31 37.45993688731231 40.0 36.5 41.0 32.0 41.0 32-33 37.262120107827215 39.0 36.0 41.0 31.0 41.0 34-35 37.07094778594827 39.0 36.0 41.0 31.0 41.0 36-37 36.875741735514225 39.0 35.0 41.0 30.5 41.0 38-39 36.86624122236637 39.0 35.5 41.0 30.5 41.0 40-41 36.88705877620455 39.5 35.5 41.0 31.0 41.0 42-43 36.782311245480514 40.0 35.0 41.0 30.5 41.0 44-45 36.619001343870465 40.0 35.0 41.0 30.0 41.0 46-47 36.342599105815374 40.0 35.0 41.0 30.0 41.0 48-49 36.073262111903816 39.0 35.0 41.0 29.0 41.0 50-51 35.88733903591717 39.0 35.0 41.0 28.0 41.0 52-53 35.74832413160509 39.0 35.0 41.0 28.0 41.0 54-55 35.57327693205859 39.0 35.0 41.0 27.5 41.0 56-57 35.37912496513294 39.0 35.0 41.0 27.0 41.0 58-59 35.176084610380784 38.5 34.5 40.5 26.5 41.0 60-61 34.956024631097236 38.0 34.0 40.0 26.0 41.0 62-63 34.74472204005782 38.0 34.0 40.0 26.0 41.0 64-65 34.5063676382866 37.0 34.0 40.0 26.0 41.0 66-67 34.23605383738797 37.0 34.0 40.0 26.0 41.0 68-69 33.93696776457549 36.5 33.5 39.5 26.0 41.0 70-71 33.61279090508273 36.0 33.0 39.0 25.5 41.0 72-73 33.338341222419295 36.0 33.0 39.0 25.5 40.5 74-75 33.14996964514727 35.0 33.0 38.5 26.0 40.0 76-77 32.847511722477776 35.0 33.0 37.0 25.0 39.5 78-79 32.506593910292544 35.0 33.0 37.0 25.0 39.0 80-81 32.22322302374559 35.0 33.0 36.5 25.0 39.0 82-83 31.955896542616678 35.0 32.5 36.0 24.0 37.5 84-85 31.698506710619014 35.0 32.0 36.0 24.0 37.0 86-87 31.489166996150466 35.0 32.0 35.0 24.0 37.0 88-89 31.323773539566375 35.0 32.0 35.0 23.0 36.0 90-91 31.18300138833093 35.0 32.0 35.0 23.0 36.0 92-93 31.04847090406649 35.0 32.0 35.0 23.0 36.0 94-95 30.927492657407246 35.0 32.0 35.0 21.0 36.0 96-97 30.862111956742083 35.0 32.0 35.0 20.0 35.0 98-99 30.784482662800723 35.0 32.0 35.0 20.0 35.0 100-101 30.698936230461875 35.0 32.0 35.0 20.0 35.0 102-103 29.686856481091866 33.5 29.5 34.5 18.5 35.0 104-105 30.441092001932972 34.0 31.5 35.0 19.0 35.0 106-107 30.593839896809378 34.5 32.0 35.0 19.0 35.0 108-109 30.56228540548208 35.0 32.0 35.0 19.0 35.0 110-111 30.528725429638932 35.0 32.0 35.0 19.0 35.0 112-113 30.451629131978244 35.0 32.0 35.0 18.0 35.0 114-115 30.426638645988316 35.0 32.0 35.0 18.0 35.0 116-117 30.330859161422715 35.0 32.0 35.0 16.0 35.0 118-119 30.199134185386374 34.0 32.0 35.0 8.5 35.0 120-121 30.003174424223758 34.0 32.0 35.0 2.0 35.0 122-123 29.775758884550463 34.0 32.0 35.0 2.0 35.0 124-125 27.685605236595833 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11003644434144988 1101 2 0.6064917856577239 1101 3 0.30256304812592205 1101 4 0.17158126948854857 1101 5 0.41793086580387495 1101 6 0.3319973686615043 1101 7 0.422410868820954 1101 8 0.39665256607310795 1101 9 0.32821593558790596 1101 10-11 0.3159900546882284 1101 12-13 0.33647261565761255 1101 14-15 0.44083194252435476 1101 16-17 0.5272690337349744 1101 18-19 0.44968966582212033 1101 20-21 0.3458897415938651 1101 22-23 0.36435989579504735 1101 24-25 0.36354254952309617 1101 26-27 0.43224361051371574 1101 28-29 0.4780275766088664 1101 30-31 0.47395592952725707 1101 32-33 0.4697035571398871 1101 34-35 0.4402881226617694 1101 36-37 0.4739858380905986 1101 38-39 0.5631357065439033 1101 40-41 0.5235574863943526 1101 42-43 0.5270952261880595 1101 44-45 0.4930606466034959 1101 46-47 0.5466533515058458 1101 48-49 0.46347810552088475 1101 50-51 0.3789919509556441 1101 52-53 0.42099378316491 1101 54-55 0.40144443860473444 1101 56-57 0.30818586790686453 1101 58-59 0.43238752663931024 1101 60-61 0.5646412921088881 1101 62-63 0.349286619044193 1101 64-65 0.3317423842554561 1101 66-67 0.4450344111226556 1101 68-69 0.4101020421068782 1101 70-71 0.40021774502599783 1101 72-73 0.45689984697435904 1101 74-75 0.43886455909532884 1101 76-77 0.3452062339931814 1101 78-79 0.3080582331485857 1101 80-81 0.29703421962611287 1101 82-83 0.22750956400273736 1101 84-85 0.24142279559502455 1101 86-87 0.3017319563229073 1101 88-89 0.299999286946548 1101 90-91 0.3631693320812026 1101 92-93 0.39369094312500863 1101 94-95 0.3542104290799344 1101 96-97 0.3296591750523987 1101 98-99 0.36188480247546195 1101 100-101 0.35487280970430035 1101 102-103 0.35134535844025905 1101 104-105 0.2165753325704678 1101 106-107 0.1809534183788628 1101 108-109 0.0927124479392134 1101 110-111 0.14891024365756778 1101 112-113 0.26346834761306326 1101 114-115 0.26175267137230307 1101 116-117 0.3215506958910943 1101 118-119 0.37573250309835515 1101 120-121 0.3778207393763253 1101 122-123 0.29308555044872975 1101 124-125 0.4000237529724444 1102 1 0.1643447812675447 1102 2 0.6042541937697408 1102 3 0.3578669923765041 1102 4 0.22546949133685246 1102 5 0.3691924675956031 1102 6 0.2982390967493913 1102 7 0.3985409651834857 1102 8 0.2898446477129113 1102 9 0.3066560337302775 1102 10-11 0.41188134988316705 1102 12-13 0.3680805662407849 1102 14-15 0.37775672999099186 1102 16-17 0.39732322869476633 1102 18-19 0.4527394304670196 1102 20-21 0.4300674457963538 1102 22-23 0.433878039522142 1102 24-25 0.38202835884822406 1102 26-27 0.27560076031348757 1102 28-29 0.4274129474467827 1102 30-31 0.25631904872506794 1102 32-33 0.18253126210549198 1102 34-35 0.311816277773616 1102 36-37 0.3259912076685083 1102 38-39 0.2869555429296824 1102 40-41 0.25087715334557004 1102 42-43 0.20145985233785524 1102 44-45 0.33407486017182464 1102 46-47 0.3012131951831023 1102 48-49 0.24432778912638042 1102 50-51 0.2177935890487035 1102 52-53 0.29884133658843126 1102 54-55 0.32239630392830065 1102 56-57 0.32251964124600363 1102 58-59 0.3019225162810244 1102 60-61 0.35036016799064384 1102 62-63 0.2256246385177434 1102 64-65 0.2805186398699462 1102 66-67 0.16614449951894983 1102 68-69 0.16229789215942247 1102 70-71 0.1932184052068351 1102 72-73 0.14327619549383996 1102 74-75 0.2077141678811003 1102 76-77 0.1352825558552624 1102 78-79 0.07786185086612107 1102 80-81 0.06191195649911663 1102 82-83 0.056902621076083904 1102 84-85 0.09916151385272443 1102 86-87 0.04357697872809041 1102 88-89 -0.013381372543467052 1102 90-91 0.018491132083248374 1102 92-93 0.042373123257178236 1102 94-95 0.04235267240264662 1102 96-97 -0.0014993178074220737 1102 98-99 0.08353239948262825 1102 100-101 0.10079292541324136 1102 102-103 0.2560417768129106 1102 104-105 0.11677149337734605 1102 106-107 0.024173207417060638 1102 108-109 0.02833167702288364 1102 110-111 0.0834479719079475 1102 112-113 0.08408075857672515 1102 114-115 0.13689133746274962 1102 116-117 0.21933311713328862 1102 118-119 0.12952716585817825 1102 120-121 0.16146009726239186 1102 122-123 0.12016017130950729 1102 124-125 0.06823388257696905 1103 1 0.10700665999732095 1103 2 0.5703439486063289 1103 3 0.24575943600660466 1103 4 0.14754357319501565 1103 5 0.4080126704425311 1103 6 0.2183500056627068 1103 7 0.341114308435877 1103 8 0.3202858066874441 1103 9 0.3611823145319448 1103 10-11 0.37063845760387437 1103 12-13 0.37420166610834116 1103 14-15 0.4590083994344667 1103 16-17 0.6047329769021843 1103 18-19 0.4214560849971889 1103 20-21 0.48953886700719096 1103 22-23 0.4299891331447654 1103 24-25 0.5122274745581947 1103 26-27 0.35352048675854064 1103 28-29 0.3822215464752716 1103 30-31 0.18364565691124568 1103 32-33 0.2886012775812645 1103 34-35 0.29967887395324055 1103 36-37 0.24458596099825058 1103 38-39 0.18071505326020798 1103 40-41 0.16741070817576542 1103 42-43 0.2532655478656167 1103 44-45 0.21370260378790107 1103 46-47 0.24232853850863734 1103 48-49 0.25880895271170345 1103 50-51 0.2212910876032126 1103 52-53 0.20980410030264096 1103 54-55 0.28236628982280365 1103 56-57 0.3250187720937987 1103 58-59 0.33571257308723546 1103 60-61 0.2645164005873468 1103 62-63 0.16866600460743086 1103 64-65 0.15988326530585084 1103 66-67 0.11301823658640586 1103 68-69 0.07519512712124055 1103 70-71 0.06833928795994382 1103 72-73 0.07520740076606103 1103 74-75 0.06403491814346296 1103 76-77 0.03300886063673403 1103 78-79 0.16149552971543102 1103 80-81 0.25021864200397204 1103 82-83 0.16605095144743132 1103 84-85 0.154669728324766 1103 86-87 0.17921940603988773 1103 88-89 0.21453475461616378 1103 90-91 0.16316365729097626 1103 92-93 0.07118615530910688 1103 94-95 0.17536935734735337 1103 96-97 0.22868366477111124 1103 98-99 0.17105556337255834 1103 100-101 0.20363353701863574 1103 102-103 0.2641526102685319 1103 104-105 0.16011053541653553 1103 106-107 0.1700979598996426 1103 108-109 0.15655101215269696 1103 110-111 0.2454166259511119 1103 112-113 0.16135782161537549 1103 114-115 0.2091722219935086 1103 116-117 0.1887614695956863 1103 118-119 0.18971005353593995 1103 120-121 0.1619145215132889 1103 122-123 0.17626163134632833 1103 124-125 0.24732529794561486 1104 1 0.10054381495564257 1104 2 0.33434175043435843 1104 3 0.19577754075077536 1104 4 0.09916733100840247 1104 5 0.32001081242029983 1104 6 0.08223570437781547 1104 7 0.21699211421403675 1104 8 0.1826646054833887 1104 9 0.2657661980178574 1104 10-11 0.20266986476310933 1104 12-13 0.2220452356323861 1104 14-15 0.19481423428263156 1104 16-17 0.2915337040179722 1104 18-19 0.22863850218170256 1104 20-21 0.2714951120466722 1104 22-23 0.2574232611361609 1104 24-25 0.20786569422177337 1104 26-27 0.04130577363643795 1104 28-29 0.11969498370667253 1104 30-31 0.11750984938479547 1104 32-33 0.024010271067211875 1104 34-35 -0.02978975180523946 1104 36-37 0.15928358433910716 1104 38-39 0.2192811300901596 1104 40-41 0.21422107286748826 1104 42-43 0.15186000708239789 1104 44-45 0.2597778061404199 1104 46-47 0.22227090507301028 1104 48-49 0.17613130676734556 1104 50-51 0.053217569540528586 1104 52-53 -0.02357565310709475 1104 54-55 -0.04904882452968451 1104 56-57 -0.021808234332468146 1104 58-59 -0.03937955080041888 1104 60-61 0.01452851613584727 1104 62-63 -0.018352116595600876 1104 64-65 0.005094331876449587 1104 66-67 -0.0017989877917159447 1104 68-69 0.03972765896912733 1104 70-71 -0.017988338416664362 1104 72-73 0.00970548788097858 1104 74-75 0.03448806708881591 1104 76-77 -0.0068407785485149475 1104 78-79 -0.021164422956211126 1104 80-81 0.04309465376657329 1104 82-83 0.03979253457015375 1104 84-85 0.04705576901838171 1104 86-87 0.03067409480287253 1104 88-89 -0.02192143373169486 1104 90-91 0.08128929507173766 1104 92-93 0.05898945549307655 1104 94-95 0.028765048418243566 1104 96-97 -0.011243971115053597 1104 98-99 0.0707561950269664 1104 100-101 0.057583342986983865 1104 102-103 0.005373866701205543 1104 104-105 0.012580415705773618 1104 106-107 0.14403467193179864 1104 108-109 0.022863706687257235 1104 110-111 -0.09263575162241011 1104 112-113 0.05921871865972861 1104 114-115 0.10017731875331037 1104 116-117 0.14547330058881514 1104 118-119 0.12165450306027736 1104 120-121 0.07884620627860528 1104 122-123 0.14461033237665077 1104 124-125 0.0929087461861613 1105 1 0.21836048877694836 1105 2 0.49407392840580755 1105 3 0.21959641766126836 1105 4 0.12754672852470605 1105 5 0.2873469554123105 1105 6 0.2895228403348753 1105 7 0.40910376349807365 1105 8 0.2671052206204507 1105 9 0.22159675724072514 1105 10-11 0.06838964405821457 1105 12-13 0.1553289477116735 1105 14-15 0.15514608584049938 1105 16-17 0.2978600483028515 1105 18-19 0.19427749929015192 1105 20-21 0.23378577132650946 1105 22-23 0.36103195586881043 1105 24-25 0.24989673006066937 1105 26-27 0.1557676974516653 1105 28-29 0.21137933448382995 1105 30-31 0.132373839226112 1105 32-33 0.07381663455713294 1105 34-35 0.09763433251332287 1105 36-37 0.20396515560607043 1105 38-39 0.15040877561958865 1105 40-41 0.011437872862444465 1105 42-43 0.06997014489246567 1105 44-45 0.10211349915505963 1105 46-47 0.1068704409663539 1105 48-49 -0.07222543213868704 1105 50-51 0.017331480860782733 1105 52-53 -0.019032006652508926 1105 54-55 0.018765914388950478 1105 56-57 -0.027608555076334085 1105 58-59 0.041067233976313844 1105 60-61 -0.031038385598996854 1105 62-63 -0.14995806556636637 1105 64-65 -0.19652078313326626 1105 66-67 -0.1402442552447667 1105 68-69 -0.10050103752744377 1105 70-71 -0.1746196098637114 1105 72-73 -0.20860952462389548 1105 74-75 -0.13037329038490242 1105 76-77 -0.04505251899797713 1105 78-79 -0.04687455473658275 1105 80-81 -0.047278744201513234 1105 82-83 -0.028545134295107744 1105 84-85 0.10417432095522017 1105 86-87 0.1251700166580072 1105 88-89 0.10643505993666835 1105 90-91 0.05886810410764198 1105 92-93 0.03848246824453838 1105 94-95 0.11691555819760069 1105 96-97 0.037625933228756736 1105 98-99 0.07550345623972987 1105 100-101 0.10025767771794492 1105 102-103 0.06456833296955011 1105 104-105 0.04318433257875398 1105 106-107 0.12256765797259916 1105 108-109 0.11141210895262432 1105 110-111 0.15764407805215797 1105 112-113 0.13991732201998275 1105 114-115 -0.018668284562846083 1105 116-117 0.033607577434686675 1105 118-119 0.04879315548605945 1105 120-121 0.07320596498274057 1105 122-123 0.05194570590736447 1105 124-125 0.07589701437627383 1106 1 0.058369552221058996 1106 2 0.40824344837895055 1106 3 0.18815734414666707 1106 4 0.12072229079392116 1106 5 0.3148034844489018 1106 6 0.2275601011606554 1106 7 0.2615381374490653 1106 8 0.2082794401572059 1106 9 0.051585679697929265 1106 10-11 0.16249202740833368 1106 12-13 0.15927624546259977 1106 14-15 0.15093040943901315 1106 16-17 0.16513111241832945 1106 18-19 0.19159069163657705 1106 20-21 0.11372031311905317 1106 22-23 0.09002960608700761 1106 24-25 0.05327454140675769 1106 26-27 0.14305823118782257 1106 28-29 0.07315879067718356 1106 30-31 0.1207113685983856 1106 32-33 0.0920455704416554 1106 34-35 0.25810747786410104 1106 36-37 0.29667009014785606 1106 38-39 0.08592992365584706 1106 40-41 0.052618302536835415 1106 42-43 0.1655065665104516 1106 44-45 0.19833857468375982 1106 46-47 0.2637007531516531 1106 48-49 0.313909680315156 1106 50-51 0.22531587159593158 1106 52-53 0.1603197892521635 1106 54-55 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2216 120-121 -0.7641864017393551 2216 122-123 -0.8906557155600723 2216 124-125 -1.0417672222122931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62.0 3 77.0 4 221.0 5 392.0 6 986.0 7 2001.0 8 3143.0 9 5168.0 10 8347.0 11 10527.0 12 11556.0 13 14186.0 14 19119.0 15 15241.0 16 7617.0 17 6545.0 18 6382.0 19 5904.0 20 6066.0 21 6368.0 22 6926.0 23 8368.0 24 10708.0 25 13723.0 26 17698.0 27 22730.0 28 29490.0 29 37154.0 30 47013.0 31 59272.0 32 74438.0 33 95996.0 34 129447.0 35 180414.0 36 299844.0 37 502435.0 38 222664.0 39 1091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.1073566018749 18.688152110587364 10.753720671574598 22.450770615963137 2 18.903253295506573 21.68240870368361 36.955616188936204 22.458721811873616 3 17.52628993773408 28.054279961636908 32.307776994425495 22.111653106203516 4 11.298786122823156 16.58656721631827 37.65072899321342 34.46391766764515 5 10.450954034932732 42.215238077079114 35.29812730987889 12.035680578109261 6 30.014095025773834 37.87756329132348 18.379744235886054 13.728597447016625 7 26.000003175747448 35.05665268808497 22.67870063234425 16.264643503823333 8 27.229176226989726 33.65011414165633 20.42900113744688 18.691708493907065 9 27.19630724086298 11.658274754025127 19.99773463348434 41.14768337162755 10-11 24.041599115871907 26.03236933519432 30.01351280540766 19.912518743526107 12-13 25.10428360695044 25.542325038810283 29.957593185692833 19.395798168546445 14-15 22.439387535770173 26.77364880511147 27.555544499246686 23.23141915987167 16-17 21.23409643958731 28.961172486890106 28.103058379547114 21.70167269397547 18-19 20.44998753519125 27.147400730104337 31.319777126043828 21.08283460866058 20-21 21.745666030987884 26.37659389441381 31.367386873259623 20.51035320133868 22-23 21.498592879233204 26.859492759031163 30.92116789171125 20.720746470024384 24-25 21.02848196320525 26.836502156730056 31.26476557552366 20.87025030454103 26-27 20.953052396127916 26.83789767635852 31.291671761094868 20.917378166418693 28-29 20.913064442796582 26.841761502424948 31.2752108034694 20.96996325130907 30-31 21.04890704004988 26.63165934392233 31.401737874863905 20.91769574116388 32-33 20.707196614229783 26.957808607228316 31.213998271334802 21.120996507207092 34-35 21.29405170342251 27.01022699244276 30.99045473648107 20.70526656765366 36-37 21.332739468559836 26.839273833587658 30.667478599431856 21.160508098420646 38-39 21.187687203696147 26.940209673432598 30.697410019165638 21.17469310370562 40-41 21.298743779501386 26.936465962850086 30.72182660621452 21.04296365143401 42-43 21.24101639042299 26.951728547579652 30.447859865747372 21.359395196249988 44-45 21.301092483837184 27.1132748286187 30.161176898110277 21.424455789433843 46-47 21.465240381281486 27.392789064439576 29.405066849187158 21.73690370509178 48-49 21.580877507150053 27.394533088213883 29.188411489418243 21.83617791521782 50-51 21.590174941952007 27.545701960570508 29.13812127498353 21.72600182249396 52-53 21.76976109180692 27.442907666928594 29.057375389616286 21.7299558516482 54-55 21.449882476367574 27.658375037615237 29.044865417509953 21.84687706850724 56-57 21.603548325798513 27.7890081085988 28.820884074932156 21.78655949067053 58-59 21.89917038792774 27.343613744348637 28.854102129594366 21.90311373812926 60-61 21.83383184802245 27.42501239890985 29.24488654137372 21.496269211693985 62-63 21.499847165010184 27.431194481532106 29.296442980639576 21.772515372818134 64-65 21.44985874304467 27.32548645984267 29.299219944026518 21.925434853086152 66-67 21.45850552290184 27.83152430079457 28.882406665645107 21.827563510658486 68-69 21.78379179480122 27.447665525144366 28.917662190558357 21.850880489496056 70-71 21.520899546304683 27.423305430925858 29.059683839760837 21.99611118300862 72-73 21.60389060633785 27.517952053929868 28.828935643610208 22.04922169612207 74-75 21.291428979742523 27.684132106954817 28.865308758126346 22.159130155176314 76-77 21.30491302097172 27.626996367978506 28.84556551453285 22.22252509651692 78-79 21.516989764538874 27.667634919341094 28.642194997761095 22.17318031835894 80-81 21.37231306831369 27.666077473400954 28.49455607123566 22.467053387049692 82-83 21.578202458670184 27.731943975432355 28.470095494978604 22.219758070918864 84-85 21.48391093792633 27.5949730894032 28.441392539028104 22.479723433642363 86-87 21.165531331544322 27.588511149573126 28.580248873927516 22.66570864495504 88-89 21.413267107361552 27.458821810744677 28.608645898007133 22.519265183886635 90-91 21.517537788916663 27.483389814360255 28.513730778035516 22.485341618687567 92-93 21.558946037664047 27.451657577362298 28.35860316089194 22.63079322408172 94-95 21.30202742614694 27.228136254215862 28.553235815321294 22.916600504315905 96-97 21.344185763397263 27.407855352686333 28.50406370664034 22.74389517727606 98-99 21.315529768015352 27.29755629710613 28.248048401392168 23.138865533486356 100-101 21.303371964053344 27.210746628578477 28.37411432120741 23.11176708616077 102-103 21.30554771902171 27.131941529636123 28.311056111287837 23.251454640054327 104-105 21.141193943583396 27.05062504565178 28.47810990513957 23.33007110562525 106-107 21.18649450459296 27.05971243393735 28.408330047292523 23.34546301417717 108-109 21.252920396888157 27.249750965702606 28.021095666220997 23.476232971188242 110-111 21.030678301666835 27.304273356341863 28.05235485427271 23.612693487718598 112-113 21.309348020393834 27.02325789443177 28.1232523097633 23.544141775411102 114-115 20.848654227481493 27.283257711579225 28.034648801562184 23.8334392593771 116-117 20.913192574073314 27.254665027284076 28.025183669525884 23.80695872911673 118-119 21.006991222725524 27.166381784177823 27.854572827133588 23.972054165963065 120-121 20.82443078615281 27.206845837127915 27.91404751148167 24.054675865237602 122-123 20.975993453289316 27.1523608228487 27.709000615606772 24.16264510825522 124-125 20.868936707641314 27.05178918038531 27.51073592922359 24.568538182749787 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 50.0 1 51.5 2 179.0 3 364.0 4 367.5 5 374.5 6 579.5 7 1194.5 8 1835.5 9 2263.0 10 2265.5 11 2019.5 12 2123.0 13 2312.5 14 2511.5 15 2751.5 16 2973.0 17 3196.0 18 3513.0 19 3849.0 20 4351.5 21 5151.5 22 6223.5 23 7788.0 24 9987.5 25 12483.0 26 15733.0 27 18848.0 28 21407.5 29 24649.5 30 29399.5 31 33950.0 32 37811.5 33 42382.0 34 46972.5 35 50614.5 36 54488.0 37 59071.5 38 60635.5 39 59753.5 40 58832.5 41 60250.5 42 62130.5 43 62858.5 44 65015.0 45 65951.5 46 64681.5 47 64395.5 48 65468.5 49 65376.0 50 63983.5 51 60955.0 52 57694.5 53 55832.0 54 53409.0 55 49383.5 56 44354.5 57 39391.5 58 35437.0 59 31787.0 60 27272.0 61 22202.0 62 17655.5 63 13542.0 64 10310.0 65 8217.5 66 6309.0 67 4955.5 68 3922.5 69 2740.0 70 1946.5 71 1589.0 72 1347.0 73 1146.5 74 850.5 75 649.0 76 440.0 77 272.5 78 168.5 79 70.5 80 27.5 81 15.0 82 14.5 83 5.0 84 0.5 85 2.0 86 2.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06452060239694832 2 2.1171649679064256E-4 3 1.587873725929819E-4 4 5.292912419766064E-5 5 0.06526161013571558 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.646456209883032E-5 16-17 7.939368629649095E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.646456209883032E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.646456209883032E-4 36-37 0.0 38-39 0.0 40-41 5.292912419766064E-5 42-43 0.002381810588894729 44-45 0.009712494290270728 46-47 0.011432690826694698 48-49 0.009103809361997629 50-51 0.007912904067550266 52-53 0.006377959465818107 54-55 0.006589675962608749 56-57 0.004763621177789458 58-59 0.00330807026235379 60-61 0.0021171649679064255 62-63 0.001402621791238007 64-65 0.00166726741222631 66-67 0.0026199916477842016 68-69 0.0015349446017321586 70-71 0.0029375663929701655 72-73 0.002672920771981862 74-75 8.733305492614004E-4 76-77 6.880786145695883E-4 78-79 2.646456209883032E-4 80-81 6.61614052470758E-4 82-83 2.646456209883032E-4 84-85 3.9696843148245473E-4 86-87 4.763621177789457E-4 88-89 0.001587873725929819 90-91 0.0021965586542029166 92-93 5.557558040754367E-4 94-95 0.0014290863533368372 96-97 0.0017995902227204619 98-99 0.001826054784819292 100-101 0.0010321179218543825 102-103 0.0010585824839532128 104-105 8.997951113602309E-4 106-107 7.41007738767249E-4 108-109 0.0011115116081508733 110-111 0.0010321179218543825 112-113 0.01156501363718885 114-115 0.022362554973511622 116-117 0.020986397744372443 118-119 0.01249127331064791 120-121 0.005954526472236822 122-123 0.00666906964890524 124-125 0.005398770668161385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1889319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.221096980101983 #Duplication Level Percentage of deduplicated Percentage of total 1 39.59096364065055 10.381184971571896 2 16.766855746782717 8.792907011755396 3 12.375680251202986 9.735117361845791 4 8.908579742514263 9.343709335337538 5 6.31744789953645 8.28252070202434 6 4.175594831169303 6.5693206210621735 7 2.8804402362287203 5.28698119456788 8 1.9212423162846242 4.0301664878059915 9 1.4041166748307317 3.313573155190344 >10 5.277841314898686 22.705792968803422 >50 0.2535534497402852 4.528221132758179 >100 0.12400689509761771 5.521074645349245 >500 0.002451334042070004 0.4418485261885265 >1k 0.001021389184195835 0.6873853966029045 >5k 2.0427783683916702E-4 0.38019648913639964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7098 0.3756909235549952 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4897 0.25919392119594414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4772 0.25257778067123654 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.292912419766064E-5 0.0 14-15 0.0 0.0 0.0 1.323228104941516E-4 0.0 16-17 0.0 0.0 0.0 1.587873725929819E-4 0.0 18-19 0.0 0.0 0.0 1.587873725929819E-4 0.0 20-21 0.0 0.0 0.0 1.587873725929819E-4 0.0 22-23 0.0 0.0 0.0 1.8525193469181222E-4 0.0 24-25 0.0 0.0 0.0 2.646456209883032E-4 0.0 26-27 0.0 0.0 0.0 2.646456209883032E-4 0.0 28-29 0.0 0.0 0.0 3.7050386938362444E-4 0.0 30-31 0.0 0.0 0.0 0.0023024169025982377 0.0 32-33 0.0 0.0 0.0 0.007754116694957284 0.0 34-35 0.0 0.0 0.0 0.014608438278554335 0.0 36-37 0.0 0.0 0.0 0.028528797942539083 0.0 38-39 0.0 0.0 0.0 0.053405486315439586 0.0 40-41 0.0 0.0 0.0 0.07878500136821787 0.0 42-43 0.0 0.0 0.0 0.10929864146816921 0.0 44-45 0.0 0.0 0.0 0.14825447687764745 0.0 46-47 0.0 0.0 0.0 0.19784906625085547 0.0 48-49 0.0 0.0 0.0 0.2504341511412313 0.0 50-51 0.0 0.0 0.0 0.3035749918356826 5.292912419766064E-5 52-53 0.0 0.0 0.0 0.36428469729039936 5.292912419766064E-5 54-55 0.0 0.0 0.0 0.43902062065749614 5.292912419766064E-5 56-57 0.0 0.0 0.0 0.5296088167217923 5.292912419766064E-5 58-59 0.0 0.0 0.0 0.6327147506588353 5.292912419766064E-5 60-61 0.0 0.0 0.0 0.7370645190145233 5.292912419766064E-5 62-63 0.0 0.0 0.0 0.8468395226004714 5.292912419766064E-5 64-65 0.0 0.0 0.0 0.9723874051973225 5.292912419766064E-5 66-67 0.0 0.0 0.0 1.1299309433716593 5.292912419766064E-5 68-69 0.0 0.0 0.0 1.312933390285071 5.292912419766064E-5 70-71 0.0 0.0 0.0 1.501361072428743 5.292912419766064E-5 72-73 0.0 0.0 0.0 1.6898681482587112 5.292912419766064E-5 74-75 0.0 0.0 0.0 1.9011082829315749 5.292912419766064E-5 76-77 0.0 0.0 0.0 2.149081229797615 5.292912419766064E-5 78-79 0.0 0.0 0.0 2.4397150507669694 5.292912419766064E-5 80-81 0.0 0.0 0.0 2.7377324845618976 5.292912419766064E-5 82-83 0.0 0.0 0.0 3.04855876641266 5.292912419766064E-5 84-85 0.0 0.0 0.0 3.38341487064916 5.292912419766064E-5 86-87 0.0 0.0 0.0 3.748255323743635 5.292912419766064E-5 88-89 0.0 0.0 0.0 4.155412611634139 5.292912419766064E-5 90-91 0.0 0.0 0.0 4.602954821287458 5.292912419766064E-5 92-93 0.0 0.0 0.0 5.046342094691262 5.292912419766064E-5 94-95 0.0 0.0 0.0 5.527097329778614 5.292912419766064E-5 96-97 0.0 0.0 0.0 6.045829211477787 5.292912419766064E-5 98-99 0.0 0.0 0.0 6.608836305568302 5.292912419766064E-5 100-101 0.0 0.0 0.0 7.214186699016947 5.292912419766064E-5 102-103 0.0 0.0 0.0 7.82093971425683 5.292912419766064E-5 104-105 0.0 0.0 0.0 8.448917308299976 5.292912419766064E-5 106-107 0.0 0.0 0.0 9.133978962790296 5.292912419766064E-5 108-109 0.0 0.0 0.0 9.85497949261083 5.292912419766064E-5 110-111 0.0 0.0 0.0 10.602153474347105 5.292912419766064E-5 112-113 0.0 0.0 0.0 11.368752444663924 5.292912419766064E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5095 0.0 87.81639 1 GTATCAA 8020 0.0 55.595818 2 GTACATG 14525 0.0 48.728172 1 ACATGGG 14550 0.0 47.501125 3 TACATGG 14845 0.0 47.3993 2 CATGGGA 10025 0.0 44.77356 4 ATCAACG 10985 0.0 42.10713 4 TCAACGC 11120 0.0 41.595936 5 CAACGCA 11220 0.0 41.19902 6 AACGCAG 11385 0.0 40.54968 7 TATCAAC 12045 0.0 38.45098 3 ATGGGAG 3790 0.0 37.69699 5 ATGGGAT 4090 0.0 36.969627 5 CATGGGG 4275 0.0 35.36977 4 ACGCAGA 13475 0.0 33.95129 8 CGCAGAG 13610 0.0 33.39596 9 AGTACAT 8920 0.0 30.499027 2 GAGTACA 9330 0.0 30.267498 1 ATGGGGG 2680 0.0 30.209167 5 CATGGGC 2055 0.0 28.678604 4 >>END_MODULE