FastQCFastQC Report
Thu 26 May 2016
SRR936214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294722
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28580.9697274041299936No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12700.43091455676875157No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11120.37730471427311163No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10730.36407190504950426No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.2765317824933327No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7000.23751196042372133No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4960.16829418910023683No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4220.14318578185544345No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.13300669783728394No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG3800.12893506423002016No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3800.12893506423002016No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3690.1252027334233617No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.11061271299733308No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3130.10620177658946398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.10518386818764802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA9550.064.78871
TTGCGTG201.7722324E-459.4934790-91
TAGCGAG300.004235272359.4934657
ACGTCGT150.004237371559.49346540-41
CGTCACT859.786163E-1055.9938551
TGGGTAT752.3068424E-855.5272376
GTGTACG453.1687727E-452.8830839
GTACATG39800.051.570971
CGCAGAA1051.1277734E-1050.99442
GTGACCC350.00779391850.99449
ATGGGAG8250.049.7581755
GTTGCGT302.1934986E-549.5778988-89
TACATGG39900.049.354232
GAGAACA7300.045.6388241
GTACAAA5100.045.4950071
ATGGGAT11050.044.68745
CATGGAA4350.043.765314
CATATAG1109.598807E-943.2679753
TACAAGG1800.042.9675062
ACATGGG41350.042.443953
Produced by FastQC (version 0.11.5)