##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 294722 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.892033848847387 31.0 26.0 33.0 10.0 34.0 2 26.913280990221292 31.0 26.0 34.0 7.0 34.0 3 27.156554312199294 31.0 26.0 34.0 7.0 34.0 4 29.504638269284275 35.0 27.0 37.0 7.0 37.0 5 30.85844626461547 35.0 31.0 37.0 10.0 37.0 6 29.899888708681402 35.0 30.0 37.0 8.0 37.0 7 30.105329768391908 35.0 30.0 37.0 8.0 37.0 8 29.635945060090528 35.0 29.0 37.0 8.0 37.0 9 30.31551088822687 37.0 27.0 39.0 7.0 39.0 10-11 30.720046348762565 37.0 28.5 39.0 7.5 39.0 12-13 30.69232700646711 37.0 28.5 39.0 7.0 39.0 14-15 31.610775578341624 38.0 29.0 41.0 7.0 41.0 16-17 31.82380175215966 38.0 30.0 41.0 7.0 41.0 18-19 31.874683600138436 38.0 30.0 41.0 7.0 41.0 20-21 32.04287430188449 38.0 30.0 41.0 7.0 41.0 22-23 32.05444792041313 38.0 29.5 41.0 2.0 41.0 24-25 32.125287559123514 38.0 30.0 41.0 2.0 41.0 26-27 32.244471060864136 38.0 30.0 41.0 2.0 41.0 28-29 32.08280684848773 38.0 30.0 40.0 2.0 41.0 30-31 31.969016564762725 38.0 30.0 40.0 2.0 41.0 32-33 31.79240606401965 38.0 30.0 40.0 2.0 41.0 34-35 31.662665155638194 38.0 30.0 40.0 2.0 41.0 36-37 31.458701759624326 38.0 29.0 40.0 2.0 41.0 38-39 31.15114582555764 37.5 28.0 40.0 2.0 41.0 40-41 31.19925387314147 37.5 29.0 40.0 2.0 41.0 42-43 31.050383072861884 37.5 28.0 40.0 2.0 41.0 44-45 30.942910607284155 37.0 28.0 40.0 2.0 41.0 46-47 30.90197711741913 37.0 28.5 40.0 2.0 41.0 48-49 30.753242038259785 37.0 28.5 40.0 2.0 41.0 50-51 30.62635127340341 37.0 28.5 40.0 2.0 41.0 52-53 30.453561322195153 36.0 28.5 40.0 2.0 41.0 54-55 30.246072570082994 36.0 28.0 40.0 2.0 41.0 56-57 30.017082199496475 35.5 28.0 40.0 2.0 41.0 58-59 29.7505038646589 35.0 28.0 40.0 2.0 41.0 60-61 29.460815616072097 35.0 28.0 39.5 2.0 41.0 62-63 29.148942053867714 35.0 27.0 39.0 2.0 41.0 64-65 28.830698081581964 35.0 26.5 39.0 2.0 41.0 66-67 28.506402643847423 35.0 26.0 39.0 2.0 40.5 68-69 28.188633695482523 34.0 26.0 38.0 2.0 40.0 70-71 27.819529929900042 34.0 25.5 37.5 2.0 40.0 72-73 27.503370973324014 34.0 25.0 37.0 2.0 39.5 74-75 27.17502256363624 34.0 24.0 36.5 2.0 39.0 76-77 26.859043437544532 34.0 24.0 36.0 2.0 39.0 78-79 26.56237573034928 34.0 23.0 35.5 2.0 38.5 80-81 26.287397954682717 33.0 21.5 35.0 2.0 37.0 82-83 26.014260896709445 33.0 20.5 35.0 2.0 37.0 84-85 26.01338549548388 33.5 21.0 35.0 2.0 36.5 86-87 25.88989454468957 34.0 20.0 35.0 2.0 36.0 88-89 25.71000468237865 34.0 20.0 35.0 2.0 36.0 90-91 25.52389539973263 33.0 18.5 35.0 2.0 36.0 92-93 25.393180692313436 33.0 18.0 35.0 2.0 35.0 94-95 25.25949538887494 33.0 16.0 35.0 2.0 35.0 96-97 25.137575410047432 33.0 11.5 35.0 2.0 35.0 98-99 25.018171361486417 33.0 7.5 35.0 2.0 35.0 100-101 24.888084364248343 33.0 6.0 35.0 2.0 35.0 102-103 24.0457956989977 31.5 2.0 34.0 2.0 35.0 104-105 24.577082810241514 33.0 2.0 35.0 2.0 35.0 106-107 24.648972591119765 33.0 2.0 35.0 2.0 35.0 108-109 24.582810241515734 33.0 2.0 35.0 2.0 35.0 110-111 24.458392994075773 33.0 2.0 35.0 2.0 35.0 112-113 24.396889271924053 33.0 2.0 35.0 2.0 35.0 114-115 24.229343245499148 33.0 2.0 35.0 2.0 35.0 116-117 24.0994394717734 33.0 2.0 35.0 2.0 35.0 118-119 23.901291725761904 33.0 2.0 35.0 2.0 35.0 120-121 23.680607487734207 33.0 2.0 35.0 2.0 35.0 122-123 23.387929981474066 33.0 2.0 35.0 2.0 35.0 124-125 21.669715867834775 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6070605148882464 1101 2 0.5340446284192026 1101 3 0.4836054511628163 1101 4 0.47317512603040157 1101 5 0.4904312542106979 1101 6 0.3534269548465012 1101 7 0.36246544490233745 1101 8 0.20970592175511626 1101 9 0.21913062567682928 1101 10-11 0.40307259554246855 1101 12-13 0.35142224871995253 1101 14-15 0.4612457062618027 1101 16-17 0.34533267802648027 1101 18-19 0.12595805296528795 1101 20-21 0.19540757561222932 1101 22-23 0.39295482423970185 1101 24-25 0.44378807115679564 1101 26-27 0.4470836099786979 1101 28-29 0.5253359321831965 1101 30-31 0.5295381617413923 1101 32-33 0.5215840662427702 1101 34-35 0.5204591718548279 1101 36-37 0.5545920688232862 1101 38-39 0.6796097272729611 1101 40-41 0.5332055867420884 1101 42-43 0.7701492619197197 1101 44-45 0.6223713527383623 1101 46-47 0.4316547262546564 1101 48-49 0.5190895351834506 1101 50-51 0.2815015239029961 1101 52-53 0.3034455867433721 1101 54-55 0.32807916509203494 1101 56-57 0.23926764486840568 1101 58-59 0.2698779364492516 1101 60-61 0.2725372683750429 1101 62-63 0.4548582887278485 1101 64-65 0.25068509712805565 1101 66-67 0.11153145063120107 1101 68-69 0.2624795032658582 1101 70-71 0.030860348498627843 1101 72-73 0.1390988722874198 1101 74-75 0.06319613079624986 1101 76-77 -0.009218782749634613 1101 78-79 0.03283023366807569 1101 80-81 0.3647272754826041 1101 82-83 0.20312282452218966 1101 84-85 0.26345101332580256 1101 86-87 0.6062246587295625 1101 88-89 0.39943077698570306 1101 90-91 0.26396281799911847 1101 92-93 0.33364265097308277 1101 94-95 0.19230547354201022 1101 96-97 0.3536802685057552 1101 98-99 0.27527835621760843 1101 100-101 0.2418896256401979 1101 102-103 0.40237297824356233 1101 104-105 0.3063979147867002 1101 106-107 0.362072405439843 1101 108-109 0.08969398851746391 1101 110-111 0.16294626708921456 1101 112-113 0.06960154673808461 1101 114-115 0.05561167451127602 1101 116-117 -0.29797049952649246 1101 118-119 -0.34225728863433247 1101 120-121 -0.3620716107765283 1101 122-123 -0.4448874512763581 1101 124-125 0.01247761691573146 1102 1 0.06531273507104984 1102 2 -0.05179626002472304 1102 3 -0.1908202677314499 1102 4 -0.20336416981475125 1102 5 -0.41721563011731533 1102 6 -0.22959119595906685 1102 7 -0.5145253035634632 1102 8 -0.12556485711652954 1102 9 -0.17343384769024794 1102 10-11 -0.012497884832782802 1102 12-13 -0.15714329997574694 1102 14-15 -0.009101643423942818 1102 16-17 -0.09361461233079282 1102 18-19 0.05371172854976436 1102 20-21 -0.3100084653244224 1102 22-23 -0.07839369347540526 1102 24-25 0.12893694855079474 1102 26-27 0.10407927158390606 1102 28-29 0.23191722146513172 1102 30-31 0.21632552477603895 1102 32-33 0.2635445942662109 1102 34-35 0.18503080171929653 1102 36-37 0.13214551080189452 1102 38-39 0.1659363676126162 1102 40-41 0.21019216367443505 1102 42-43 0.28010995680449113 1102 44-45 0.4364067088019148 1102 46-47 0.4745241850677182 1102 48-49 0.3807892960155357 1102 50-51 0.4878626489192186 1102 52-53 0.2828637041766733 1102 54-55 0.15527570920831835 1102 56-57 -0.0016754924955542094 1102 58-59 0.13140851842451795 1102 60-61 -0.04006817872064161 1102 62-63 0.09950621130418824 1102 64-65 0.2619969053487594 1102 66-67 -0.12903624872174646 1102 68-69 -0.0980475931614535 1102 70-71 0.0501444206568884 1102 72-73 -0.2371039198589706 1102 74-75 -0.3428261730026243 1102 76-77 -0.3306655632528148 1102 78-79 -0.3956720004198431 1102 80-81 -0.26518122888360196 1102 82-83 -0.2132101194680196 1102 84-85 -0.09509460471005582 1102 86-87 -0.07248383451255336 1102 88-89 -0.0910697143282384 1102 90-91 -0.4330957874108954 1102 92-93 -0.4182414916377404 1102 94-95 -0.5221275687109888 1102 96-97 -0.45768530614615344 1102 98-99 -0.579610217488753 1102 100-101 -0.36148792594776324 1102 102-103 -0.21414258621126336 1102 104-105 -0.2961453301101251 1102 106-107 -0.25439680864325354 1102 108-109 -0.31864119883073627 1102 110-111 -0.6752144574597949 1102 112-113 -0.4260276132077756 1102 114-115 -0.45611960457358336 1102 116-117 -0.48033371957951587 1102 118-119 -0.4008905088727559 1102 120-121 -0.6950920791006787 1102 122-123 -0.7858917711310021 1102 124-125 -0.718185007998521 1103 1 0.2177665536539024 1103 2 0.30433828643240446 1103 3 0.009097019282613417 1103 4 -0.2381863368949837 1103 5 0.1558337219642958 1103 6 0.07588164661055075 1103 7 0.32168919878007074 1103 8 0.04760856444399053 1103 9 0.2511181236359157 1103 10-11 0.09863390452481013 1103 12-13 -0.0481275845084177 1103 14-15 0.15510431191104246 1103 16-17 0.268309843142049 1103 18-19 0.4986938074028089 1103 20-21 0.28538028570949336 1103 22-23 0.18961705921276462 1103 24-25 0.5158614040236316 1103 26-27 0.5290792715839032 1103 28-29 0.6329304555428834 1103 30-31 0.35094090939142575 1103 32-33 0.6059005358156959 1103 34-35 0.6143387697369498 1103 36-37 0.6554154308460021 1103 38-39 0.685263635326983 1103 40-41 0.7064218300653913 1103 42-43 0.6425444756906238 1103 44-45 0.5668795513715352 1103 46-47 0.4114555332893879 1103 48-49 0.4539902885713616 1103 50-51 0.24350091746071456 1103 52-53 0.42617084506445835 1103 54-55 0.5824607105868687 1103 56-57 0.24032891886369256 1103 58-59 0.40391334334870166 1103 60-61 0.40043361339405337 1103 62-63 0.3581703965812757 1103 64-65 0.616484084835939 1103 66-67 0.5618101808343603 1103 68-69 0.4939981746907627 1103 70-71 0.6063134308581581 1103 72-73 0.4152120437473137 1103 74-75 0.07745918679886188 1103 76-77 0.3813347124571358 1103 78-79 0.27195275833394916 1103 80-81 0.5938551648302131 1103 82-83 0.645557457041491 1103 84-85 0.5956484336136292 1103 86-87 0.2991456113104398 1103 88-89 0.17831958812558213 1103 90-91 0.3930277306481038 1103 92-93 0.18355751580642732 1103 94-95 0.4156791586118693 1103 96-97 0.28735880744634557 1103 98-99 0.09099220875883418 1103 100-101 0.3214525207023584 1103 102-103 0.4513385276569508 1103 104-105 0.4162409395342124 1103 106-107 0.540557699214478 1103 108-109 0.40984653207634736 1103 110-111 0.3104003757356857 1103 112-113 0.4642811819397288 1103 114-115 0.5192181401190012 1103 116-117 0.6238777499545343 1103 118-119 0.9270940513698669 1103 120-121 0.9414877389307499 1103 122-123 0.9717453945706822 1103 124-125 0.6709106633552153 1104 1 0.34728969530888065 1104 2 0.3285084594104326 1104 3 0.3350437233499335 1104 4 0.4991441913892629 1104 5 0.26085943491427344 1104 6 0.26921965502569734 1104 7 0.5939659636234573 1104 8 0.5158881343364605 1104 9 0.5594163938556456 1104 10-11 0.5707704169137777 1104 12-13 0.577667179399949 1104 14-15 0.6549173086384847 1104 16-17 0.8370802966249897 1104 18-19 0.4608373324144992 1104 20-21 0.3036481678974283 1104 22-23 0.25820050942314055 1104 24-25 0.276660843013822 1104 26-27 0.12147057593173116 1104 28-29 0.0962427510080488 1104 30-31 0.1339119238841775 1104 32-33 0.35598234974743903 1104 34-35 0.32417981695526876 1104 36-37 0.5448731213555895 1104 38-39 0.4053547994223514 1104 40-41 0.37394169916310105 1104 42-43 0.5317450367004426 1104 44-45 0.38123205254030523 1104 46-47 0.35632463099859635 1104 48-49 0.25238907305009306 1104 50-51 0.2704527641180263 1104 52-53 0.35938730135244157 1104 54-55 0.3423251332142634 1104 56-57 0.3550320511685108 1104 58-59 0.42214616242303293 1104 60-61 0.68613955074796 1104 62-63 0.6790212614714122 1104 64-65 0.7813391572997048 1104 66-67 0.664042532400515 1104 68-69 0.4189163607590203 1104 70-71 0.38815073801103495 1104 72-73 0.3339941849347845 1104 74-75 0.48323291283906755 1104 76-77 0.5774076437334301 1104 78-79 0.11669329129327366 1104 80-81 0.322583542576826 1104 82-83 0.18506189836921294 1104 84-85 0.261835904394367 1104 86-87 0.2028506136479784 1104 88-89 -0.018875362786062055 1104 90-91 0.28297630474814994 1104 92-93 0.17863699219726215 1104 94-95 0.032322449869464975 1104 96-97 0.13185389861044428 1104 98-99 0.04223110543952657 1104 100-101 0.0660761766163418 1104 102-103 0.10874853233202941 1104 104-105 0.22130171559778944 1104 106-107 0.03924867630657758 1104 108-109 -0.021920648849320656 1104 110-111 0.05666031028453844 1104 112-113 0.06741348675506487 1104 114-115 0.03247199706149928 1104 116-117 -0.007024540835555371 1104 118-119 -0.025816187059305662 1104 120-121 -0.10916677252319928 1104 122-123 -0.08957999112356774 1104 124-125 -0.3903329084072915 1105 1 0.5507900637687193 1105 2 0.5086484741488277 1105 3 0.5656443128856736 1105 4 0.5274050609544787 1105 5 0.2332986390410241 1105 6 0.3514967368237798 1105 7 0.23663361284969042 1105 8 0.06715932077714015 1105 9 0.13259251765814994 1105 10-11 0.37542222236698564 1105 12-13 0.2988812545657602 1105 14-15 0.7091730197659736 1105 16-17 0.6170637159322823 1105 18-19 0.41941323514109996 1105 20-21 0.06681139560561178 1105 22-23 0.17654980934945286 1105 24-25 0.4187113220116103 1105 26-27 0.5331046953127228 1105 28-29 0.5628602896963315 1105 30-31 0.5186668980919862 1105 32-33 0.7386278913834055 1105 34-35 0.7326912392102436 1105 36-37 0.9342062090490337 1105 38-39 1.102766369061257 1105 40-41 0.8528565849405467 1105 42-43 1.0685081170246846 1105 44-45 1.1820426641836335 1105 46-47 0.9426087135335983 1105 48-49 1.0657088961893706 1105 50-51 1.1986767876994584 1105 52-53 1.2079687309766172 1105 54-55 1.2601033203918632 1105 56-57 1.1656068485155977 1105 58-59 0.9468329715608377 1105 60-61 1.2072246649705072 1105 62-63 1.2011196402481232 1105 64-65 1.1519360622370698 1105 66-67 1.14923044691783 1105 68-69 1.215719971665429 1105 70-71 1.0914760880478767 1105 72-73 1.129204207042374 1105 74-75 1.0994543572016333 1105 76-77 1.112964018088629 1105 78-79 1.2376033870928325 1105 80-81 1.1629206833284833 1105 82-83 1.0884793633655292 1105 84-85 1.178011291277194 1105 86-87 1.1720565826973548 1105 88-89 0.9749160889457009 1105 90-91 1.0972080659861767 1105 92-93 0.964635518357916 1105 94-95 1.0334370408790434 1105 96-97 1.1162680474682176 1105 98-99 1.0289204937961962 1105 100-101 0.9370507528875258 1105 102-103 1.1984316568714561 1105 104-105 1.0131495416847471 1105 106-107 1.1141657728430552 1105 108-109 0.9970458213054378 1105 110-111 0.9093592785969911 1105 112-113 1.0008972745221953 1105 114-115 1.0992277081889839 1105 116-117 1.2906173162020274 1105 118-119 1.3141156478706861 1105 120-121 1.4764909282874115 1105 122-123 1.4113503699670744 1105 124-125 1.7820141807599867 1106 1 -0.9680792542032925 1106 2 -0.8190997626223435 1106 3 -1.062531345324878 1106 4 -1.1414402549496607 1106 5 -0.9457475557080635 1106 6 -1.2597990055786426 1106 7 -1.5700389620182982 1106 8 -1.7212578351624472 1106 9 -1.7023009500867623 1106 10-11 -1.5135243638004425 1106 12-13 -1.3628580645140396 1106 14-15 -1.4446848510659756 1106 16-17 -1.5481143609481869 1106 18-19 -1.3368111472672268 1106 20-21 -1.4296875335328991 1106 22-23 -1.2594939113403356 1106 24-25 -5.178776307691873 1106 26-27 -2.6815961096797096 1106 28-29 -2.2541100954445383 1106 30-31 -2.327708328081343 1106 32-33 -6.600101291438502 1106 34-35 -3.0265495569198855 1106 36-37 -2.1653239043145796 1106 38-39 -1.911489474519108 1106 40-41 -2.4178244275774503 1106 42-43 -2.222948531548113 1106 44-45 -2.1899339984211466 1106 46-47 -2.1321877304677272 1106 48-49 -2.0416172734498588 1106 50-51 -1.9846528530080505 1106 52-53 -2.175930497264588 1106 54-55 -2.71090495108999 1106 56-57 -2.4873047869483784 1106 58-59 -2.182002743516154 1106 60-61 -1.8625082658666123 1106 62-63 -2.06315928224366 1106 64-65 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104-105 -0.4151513241161169 2216 106-107 -0.38468318892963893 2216 108-109 -0.3554516106411505 2216 110-111 -0.43902009626543403 2216 112-113 -0.542938480118643 2216 114-115 -0.27632717478115154 2216 116-117 0.006498892253095079 2216 118-119 0.03314767516542716 2216 120-121 0.04088028187167936 2216 122-123 0.15047741523818559 2216 124-125 0.21332792351440943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20424.0 3 3747.0 4 3637.0 5 3705.0 6 4271.0 7 3026.0 8 2237.0 9 2277.0 10 2844.0 11 3339.0 12 3302.0 13 2970.0 14 2743.0 15 2471.0 16 2063.0 17 1788.0 18 1535.0 19 1387.0 20 1261.0 21 1333.0 22 1590.0 23 2755.0 24 4556.0 25 5624.0 26 6282.0 27 4400.0 28 4502.0 29 5322.0 30 6092.0 31 7610.0 32 9717.0 33 12561.0 34 17222.0 35 25653.0 36 39422.0 37 51560.0 38 19440.0 39 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.454355135874266 20.03963070157878 8.65225077276475 18.853763389782202 2 17.583409393969212 27.022845335079616 34.90012588176615 20.493619389185024 3 16.55729806393822 38.85118857771052 25.667238957390353 18.924274400960904 4 9.752001058635514 21.952775355508127 30.797471489791974 37.49775209606439 5 9.895900487924049 51.80986570212881 26.900630433159833 11.393603376787302 6 25.628566969102668 41.304569113525474 18.316831683168317 14.750032234203545 7 26.537571509035757 43.10086251942535 16.938565001119716 13.42300097041918 8 26.499569079594732 40.4176195549644 16.791644894441465 16.291166470999396 9 27.10464615207131 23.6691470122185 15.170824893032977 34.05538194267721 10-11 21.760197070409916 37.192289552350225 23.002236043390777 18.045277333849082 12-13 22.988324392552855 35.94499129673551 22.885344245496526 18.181340065215103 14-15 21.223760622568623 35.85453040145632 21.621433624745304 21.30027535122975 16-17 20.443953575566944 37.008911984608886 22.76517522831341 19.781959211510763 18-19 20.097416313075875 34.80249529629523 25.192347169888148 19.907741220740753 20-21 22.862620350341434 32.78635958773381 24.48640624337278 19.86461381855198 22-23 22.83567922051679 33.35574789056942 23.766392767569535 20.04218012134426 24-25 21.142495533813232 33.43184675338336 25.159603616381276 20.26605409642213 26-27 21.45604335013479 32.80457660158152 25.132966766366998 20.606413281916687 28-29 21.379117290286462 32.44208436892957 25.287945235691804 20.890853105092162 30-31 21.277895215442506 32.93849395525908 25.399108981775857 20.384501847522554 32-33 20.46084966399913 33.26518874918778 25.193194137416807 21.080767449396284 34-35 20.931668694024978 32.57633979543191 26.08624840313723 20.40574310740588 36-37 21.150342188035467 32.86413251855144 25.016710719629753 20.96881457378334 38-39 21.299852740585916 32.80474894645051 25.480968247612974 20.414430065350604 40-41 20.908086869334635 33.15146487241215 25.599852062222084 20.34059619603113 42-43 20.53569762332531 33.289223445423346 25.50501580321561 20.670063128035736 44-45 20.676679611222234 33.19286030568152 25.441904732821317 20.688555350274925 46-47 20.773417300606003 33.32965750310466 25.11163213648301 20.785293059806325 48-49 20.796992404668153 33.544833155481214 24.495242102262992 21.162932337587648 50-51 20.94452682046288 33.64730472076792 24.776142698638363 20.632025760130837 52-53 20.73558452486784 33.92361511682354 24.796924517674523 20.543875840634097 54-55 20.747869990533292 33.822276964002754 24.90813220818616 20.521720837277798 56-57 20.64366724066267 34.10333624572684 24.483785319840187 20.769211193770307 58-59 20.728388736389576 33.1980632398997 24.954278482215262 21.11926954149546 60-61 20.707422010192793 34.071213838313234 24.636432114767338 20.584932036726634 62-63 20.51014954999279 34.20512864013844 24.480392240026465 20.804329569842306 64-65 20.373543522960933 34.29261192054778 24.74534979200456 20.58849476448673 66-67 20.463625706389614 34.56936317629754 24.119971294836972 20.84703982247587 68-69 21.010584604735673 34.599561279245926 24.006304278121135 20.38354983789726 70-71 20.49735595152663 34.12646304863794 24.460968687069187 20.915212312766247 72-73 20.80988597671353 34.68862871771729 24.172563496601015 20.328921808968158 74-75 20.541905099594697 34.963075059505734 24.187574965348407 20.30744487555116 76-77 20.07420627784432 34.95804478163946 24.86792504046227 20.099823900053952 78-79 20.5978555917481 34.46525515743757 24.45100434310532 20.48588490770901 80-81 19.857695198315685 35.34632082369847 24.079675759493348 20.7163082184925 82-83 20.414222264446714 34.76915455634312 23.818289161613865 20.998334017596303 84-85 20.558291669564895 34.062951168988924 24.620275820650413 20.758481340795772 86-87 20.283963959073088 34.61771407146772 24.206493949352012 20.891828020107187 88-89 20.72305917480999 33.93017100977199 24.44235206297503 20.904417752442995 90-91 20.47169955364641 34.21263803285475 24.172820781653186 21.14284163184566 92-93 20.21196461714379 34.603639398885036 24.04578598597308 21.138609997998092 94-95 19.867637303818363 34.320992262160246 24.390057698734903 21.42131273528648 96-97 20.439195780408895 34.62891112087554 23.957274773895946 20.974618324819616 98-99 20.183936048208142 34.69914020670327 23.575097888857975 21.541825856230616 100-101 19.604303731325558 35.505953087835614 24.159459285222297 20.730283895616534 102-103 19.999219595614807 35.745235291922135 23.998792069734222 20.256753042728835 104-105 19.077501980683458 36.255074706120865 24.05457355503416 20.61284975816152 106-107 18.930340662323562 36.71908930510315 23.77171552660152 20.57885450597177 108-109 19.016927989033622 36.93704851061519 23.052297272995634 20.99372622735555 110-111 19.071927958373838 37.44624107410971 22.993296993571832 20.488533973944627 112-113 19.42552350447288 37.29855914332393 23.181115667216908 20.094801684986283 114-115 18.7961136059419 37.89539580721396 22.964300074138038 20.344190512706106 116-117 19.101043534615116 37.6863424374702 22.86733656917849 20.345277458736188 118-119 18.998669914969565 37.540801715538244 23.1519622147273 20.308566154764893 120-121 18.849352438407852 37.259306259140004 23.07402594318016 20.81731535927199 122-123 19.229438110749186 37.20717969598263 22.86526194353963 20.698120249728554 124-125 19.711048264372515 37.52470561090932 22.26278796351119 20.501458161206973 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2212.0 1 2586.5 2 3314.5 3 3135.5 4 2099.5 5 1431.5 6 1150.0 7 991.5 8 961.5 9 966.5 10 848.5 11 687.5 12 611.5 13 563.0 14 531.5 15 538.5 16 558.0 17 609.0 18 688.0 19 766.5 20 874.5 21 1045.5 22 1231.5 23 1434.0 24 1711.5 25 2098.5 26 2549.0 27 2972.0 28 3299.0 29 3721.5 30 4340.5 31 4975.0 32 5753.5 33 6588.0 34 7385.5 35 7557.0 36 7874.0 37 8605.5 38 8603.0 39 8381.0 40 8056.0 41 7997.0 42 7834.5 43 7508.5 44 7795.0 45 8154.5 46 8270.0 47 8202.5 48 8180.0 49 8112.0 50 8285.5 51 7935.5 52 7188.0 53 7196.0 54 6972.0 55 6418.0 56 6187.5 57 5966.0 58 5889.5 59 5957.5 60 5759.5 61 5458.5 62 4762.0 63 3377.5 64 2206.5 65 1770.5 66 1588.5 67 1286.0 68 927.0 69 663.5 70 582.0 71 545.5 72 501.5 73 609.5 74 534.5 75 328.0 76 185.0 77 114.0 78 98.0 79 57.0 80 34.5 81 27.5 82 15.5 83 6.5 84 4.5 85 3.5 86 3.0 87 4.0 88 4.0 89 4.5 90 5.0 91 2.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.393028006053162E-4 2 3.393028006053162E-4 3 0.0 4 0.0010179084018159484 5 0.0013572112024212647 6 0.0013572112024212647 7 0.0013572112024212647 8 0.0013572112024212647 9 0.0016965140030265808 10-11 0.0016965140030265808 12-13 0.0016965140030265808 14-15 0.0025447710045398714 16-17 0.0022054682039345554 18-19 0.0025447710045398714 20-21 0.0032233766057505036 22-23 0.09178140756373804 24-25 0.0025447710045398714 26-27 0.001866165403329239 28-29 0.001526862602723923 30-31 0.0010179084018159484 32-33 0.001866165403329239 34-35 0.0011875598021186067 36-37 3.393028006053162E-4 38-39 0.0013572112024212647 40-41 0.0011875598021186067 42-43 0.0011875598021186067 44-45 0.0011875598021186067 46-47 0.0013572112024212647 48-49 5.089542009079742E-4 50-51 0.0010179084018159484 52-53 0.0013572112024212647 54-55 0.0016965140030265808 56-57 0.001526862602723923 58-59 0.0010179084018159484 60-61 0.0013572112024212647 62-63 0.001526862602723923 64-65 0.0013572112024212647 66-67 5.089542009079742E-4 68-69 5.089542009079742E-4 70-71 0.0011875598021186067 72-73 5.089542009079742E-4 74-75 8.482570015132904E-4 76-77 0.0010179084018159484 78-79 6.786056012106324E-4 80-81 8.482570015132904E-4 82-83 3.393028006053162E-4 84-85 5.089542009079742E-4 86-87 5.089542009079742E-4 88-89 6.786056012106324E-4 90-91 1.696514003026581E-4 92-93 0.0010179084018159484 94-95 5.089542009079742E-4 96-97 5.089542009079742E-4 98-99 0.0 100-101 3.393028006053162E-4 102-103 0.0010179084018159484 104-105 1.696514003026581E-4 106-107 6.786056012106324E-4 108-109 0.0010179084018159484 110-111 0.0011875598021186067 112-113 5.089542009079742E-4 114-115 5.089542009079742E-4 116-117 8.482570015132904E-4 118-119 0.0013572112024212647 120-121 3.393028006053162E-4 122-123 6.786056012106324E-4 124-125 5.089542009079742E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 294722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.523141187210925 #Duplication Level Percentage of deduplicated Percentage of total 1 67.85670672182617 28.176236137095522 2 10.542141965146387 8.754856984687894 3 6.557136930162663 8.168187676050573 4 4.303622075388458 7.147996282110106 5 2.9992611445343824 6.22693719809085 6 1.9820091154910104 4.937954660212336 7 1.2878567624453305 3.743310072314527 8 0.920544387505436 3.0579115657186255 9 0.6634676214162492 2.479433374548893 >10 2.74852152756863 18.887244186485663 >50 0.09792829306961197 2.778631731890579 >100 0.03590704079219106 2.988723399423769 >500 0.0016321382178268661 0.5133691553431985 >1k 0.0032642764356537323 2.1392075760274665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2858 0.9697274041299936 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1270 0.43091455676875157 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1112 0.37730471427311163 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1073 0.36407190504950426 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 815 0.2765317824933327 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 700 0.23751196042372133 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 496 0.16829418910023683 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 422 0.14318578185544345 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 392 0.13300669783728394 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 380 0.12893506423002016 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 380 0.12893506423002016 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 369 0.1252027334233617 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 326 0.11061271299733308 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 313 0.10620177658946398 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.10518386818764802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.089542009079743E-4 0.0 20-21 0.0 0.0 0.0 6.786056012106324E-4 0.0 22-23 0.0 0.0 0.0 0.0010179084018159487 0.0 24-25 0.0 0.0 0.0 0.0013572112024212647 0.0 26-27 0.0 0.0 0.0 0.0023751196042372134 0.0 28-29 0.0 0.0 0.0 0.003732330806658478 0.0 30-31 0.0 0.0 0.0 0.011027341019672777 0.0 32-33 0.0 0.0 0.0 0.0303676006541758 0.0 34-35 0.0 0.0 0.0 0.0514043742917054 0.0 36-37 0.0 0.0 0.0 0.08652221415435563 0.0 38-39 0.0 0.0 0.0 0.1311405324339547 0.0 40-41 0.0 0.0 0.0 0.19017921973927973 0.0 42-43 0.0 0.0 0.0 0.2592273396624616 0.0 44-45 0.0 0.0 0.0 0.3340436071959338 0.0 46-47 0.0 0.0 0.0 0.4181907017460522 0.0 48-49 0.0 0.0 0.0 0.5098024579094875 0.0 50-51 0.0 0.0 0.0 0.6066734074823054 0.0 52-53 0.0 0.0 0.0 0.7057498252590577 0.0 54-55 0.0 0.0 0.0 0.8204341718636545 0.0 56-57 0.0 0.0 0.0 0.9709149639321122 0.0 58-59 0.0 0.0 0.0 1.1409056670353759 0.0 60-61 0.0 0.0 0.0 1.3142893981446924 0.0 62-63 0.0 0.0 0.0 1.490048248858246 0.0 64-65 0.0 0.0 0.0 1.6788702573951046 0.0 66-67 0.0 0.0 0.0 1.9070513908021796 0.0 68-69 0.0 0.0 0.0 2.194440862914883 0.0 70-71 0.0 0.0 0.0 2.4901432536424153 0.0 72-73 0.0 0.0 0.0 2.7631123567293923 0.0 74-75 0.0 0.0 0.0 3.0523679942454245 0.0 76-77 0.0 0.0 0.0 3.389465326646806 0.0 78-79 0.0 0.0 0.0 3.805280908788621 0.0 80-81 0.0 0.0 0.0 4.209390544309553 0.0 82-83 0.0 0.0 0.0 4.613669831230787 0.0 84-85 0.0 0.0 0.0 5.035083909582589 0.0 86-87 0.0 0.0 0.0 5.502982471617321 0.0 88-89 0.0 0.0 0.0 6.015669003331953 0.0 90-91 0.0 0.0 0.0 6.587224570951609 0.0 92-93 0.0 0.0 0.0 7.147243843350683 0.0 94-95 0.0 0.0 0.0 7.738478973405446 0.0 96-97 0.0 0.0 0.0 8.349224014495016 0.0 98-99 0.0 0.0 0.0 9.014766457882343 0.0 100-101 0.0 0.0 0.0 9.754276911801629 0.0 102-103 0.0 3.393028006053162E-4 0.0 10.460026737060687 0.0 104-105 0.0 3.393028006053162E-4 0.0 11.172223315531246 0.0 106-107 0.0 3.393028006053162E-4 0.0 11.943119278506526 0.0 108-109 0.0 3.393028006053162E-4 0.0 12.721140600294515 0.0 110-111 0.0 3.393028006053162E-4 0.0 13.629284546114643 0.0 112-113 0.0 3.393028006053162E-4 0.0 14.461085361798576 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 955 0.0 64.7887 1 TTGCGTG 20 1.7722324E-4 59.49347 90-91 TAGCGAG 30 0.0042352723 59.493465 7 ACGTCGT 15 0.0042373715 59.493465 40-41 CGTCACT 85 9.786163E-10 55.993855 1 TGGGTAT 75 2.3068424E-8 55.527237 6 GTGTACG 45 3.1687727E-4 52.883083 9 GTACATG 3980 0.0 51.57097 1 CGCAGAA 105 1.1277734E-10 50.9944 2 GTGACCC 35 0.007793918 50.9944 9 ATGGGAG 825 0.0 49.758175 5 GTTGCGT 30 2.1934986E-5 49.57789 88-89 TACATGG 3990 0.0 49.35423 2 GAGAACA 730 0.0 45.638824 1 GTACAAA 510 0.0 45.495007 1 ATGGGAT 1105 0.0 44.6874 5 CATGGAA 435 0.0 43.76531 4 CATATAG 110 9.598807E-9 43.267975 3 TACAAGG 180 0.0 42.967506 2 ACATGGG 4135 0.0 42.44395 3 >>END_MODULE