FastQCFastQC Report
Thu 26 May 2016
SRR936214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294722
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68762.333046056962154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62832.131839496203202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56691.9235075766315377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.5747789442254057No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.5194725877267391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.44991551360264925No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10750.3647505106507149No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.3450709482156066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9910.33624907539986837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.30435461214296866No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4670.15845440788268267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4630.1570971966802614No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4270.14488229585847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3960.13436390903970521No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3840.1302922754324414No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3740.12689924742638825No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.10246944578280551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.076.535531
ATAACGC753.6379788E-1271.4562453
CGAGTAC359.105576E-568.0535665
CCGAGTA359.105576E-568.0535664
GTTATCA1350.061.7837831
TCGTGCG150.004236558559.4963526-27
TATAACG954.1836756E-1156.412822
CTTGCGC551.3022187E-554.1335143
CGGGAGT3650.052.2054754
TAACGCA1051.10958354E-1051.040174
ATCCACG751.459126E-647.6374972
CCACGCA751.459126E-647.6374974
CACGCAG1004.100002E-947.6374935
GTATCAA44000.046.443151
GTGGGTA4250.046.197179
TCGGGAG4150.045.9156573
GTCGGGA4100.045.023242
ATGGGCG802.2756867E-644.660155
ATGGGAT7950.040.446935
GAGTGGG4850.039.2553257
Produced by FastQC (version 0.11.5)