FastQCFastQC Report
Thu 26 May 2016
SRR936213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184726
Sequences flagged as poor quality0
Sequence length125
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44962.433875036540606No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22061.1942011411495945No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16550.8959215270183948No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.5619133202689389No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9530.515899223715124No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9410.5094031159663501No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7030.3805636456156686No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6910.37406753786689473No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5540.2999036410683932No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3890.2105821595227526No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.20895813258555918No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.16889880146812036No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG2960.1602373244697552No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2810.15211718978378788No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.14507973972261617No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT2620.14183168584822925No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2610.14129034353583145No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2250.12180202028950987No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2200.11909530872752078No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.11530591254073602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGC208.4723847E-489.2443161
CGCAGAA603.945388E-969.412252
GAACAAA9900.068.510791
TACATGA2100.065.162512
AGCGTTA150.004235347759.4962180-81
CGACATC150.004235347759.4962148-49
GGGACTA300.004231999659.496217
TGTCGAC150.004235347759.4962134-35
GAACAAG3250.058.5808871
GTACATA1350.057.292641
GAGTGCC350.00778791950.996759
GACACCC350.00778791950.996759
GTCCTAT350.00778791950.996751
GTCACTA709.1031325E-750.996751
CGGGAGA350.00778791950.996754
TACCTGG952.6138878E-950.1020742
TACAAGG952.6138878E-950.1020742
GAGAACA6900.050.0113071
AGAACAA4800.049.5801732
GATAGTC602.1869995E-549.5801737
Produced by FastQC (version 0.11.5)