##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936213_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 184726 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.430242629624416 31.0 31.0 34.0 30.0 34.0 2 31.2230546864004 31.0 31.0 34.0 28.0 34.0 3 31.503746088801794 31.0 31.0 34.0 28.0 34.0 4 35.44033325032751 37.0 35.0 37.0 33.0 37.0 5 35.15472104630642 37.0 35.0 37.0 33.0 37.0 6 35.42708660394314 37.0 35.0 37.0 33.0 37.0 7 35.24759373342139 37.0 35.0 37.0 32.0 37.0 8 35.372503058584066 37.0 35.0 37.0 33.0 37.0 9 36.93913688381711 39.0 37.0 39.0 33.0 39.0 10-11 36.82682459426394 39.0 37.0 39.0 32.0 39.0 12-13 36.73957374706322 39.0 37.0 39.0 32.0 39.0 14-15 37.43671979039226 39.0 36.0 41.0 32.0 41.0 16-17 37.479550794149176 39.0 36.5 41.0 32.0 41.0 18-19 37.99155235321503 40.0 37.0 41.0 33.0 41.0 20-21 38.1519168931282 40.0 37.5 41.0 33.0 41.0 22-23 37.924314931303655 40.0 36.5 41.0 33.0 41.0 24-25 37.75410608143954 40.0 36.0 41.0 33.0 41.0 26-27 37.37325011097517 40.0 35.5 41.0 32.0 41.0 28-29 37.06274428071847 39.5 35.0 41.0 32.0 41.0 30-31 36.270208308521816 38.5 35.0 40.0 31.0 41.0 32-33 35.28795080281065 36.5 35.0 40.0 30.0 41.0 34-35 34.619198163766875 35.0 34.0 40.0 28.0 41.0 36-37 33.99943971070667 35.0 33.5 40.0 23.5 41.0 38-39 33.66929668806773 35.0 33.0 40.0 21.5 41.0 40-41 33.24974556911317 35.0 33.0 40.0 17.5 41.0 42-43 32.71071749510085 35.0 33.0 40.0 10.0 41.0 44-45 31.809799378539026 35.0 32.5 40.0 2.0 41.0 46-47 30.364231348050627 35.0 28.5 40.0 2.0 41.0 48-49 29.221159988307008 35.0 23.0 40.0 2.0 41.0 50-51 28.930811039052436 35.0 23.0 40.0 2.0 41.0 52-53 28.887739137966502 35.0 23.0 40.0 2.0 41.0 54-55 28.753691954570552 35.0 23.0 40.0 2.0 41.0 56-57 28.600253889544515 35.0 23.0 40.0 2.0 41.0 58-59 28.4219979862066 35.0 22.0 40.0 2.0 41.0 60-61 28.21400885636023 35.0 22.0 39.0 2.0 41.0 62-63 27.988112122819743 35.0 21.5 39.0 2.0 41.0 64-65 27.764865259898443 35.0 20.0 39.0 2.0 41.0 66-67 27.51229117720299 35.0 19.5 38.0 2.0 40.5 68-69 27.25062795708238 35.0 18.5 37.5 2.0 40.0 70-71 26.942910039734524 35.0 14.0 37.0 2.0 40.0 72-73 26.62492015200892 35.0 9.0 37.0 2.0 39.5 74-75 26.354078472981605 34.5 4.5 36.0 2.0 39.0 76-77 26.006888580925263 34.0 2.0 36.0 2.0 39.0 78-79 25.66021567077726 34.0 2.0 35.0 2.0 38.0 80-81 25.35988166257051 34.0 2.0 35.0 2.0 37.0 82-83 25.060408388640475 34.0 2.0 35.0 2.0 37.0 84-85 24.750159695982155 34.0 2.0 35.0 2.0 36.0 86-87 24.463548715394694 34.0 2.0 35.0 2.0 36.0 88-89 24.18521756547535 33.0 2.0 35.0 2.0 36.0 90-91 23.91704741075972 33.0 2.0 35.0 2.0 35.5 92-93 23.68574266751838 33.0 2.0 35.0 2.0 35.0 94-95 23.489064885289565 33.0 2.0 35.0 2.0 35.0 96-97 23.283219471000294 33.0 2.0 35.0 2.0 35.0 98-99 23.09340049586956 33.0 2.0 35.0 2.0 35.0 100-101 22.89624362569427 33.0 2.0 35.0 2.0 35.0 102-103 21.963621796606866 30.5 2.0 34.0 2.0 35.0 104-105 22.40854562974351 32.0 2.0 35.0 2.0 35.0 106-107 22.38694607147884 33.0 2.0 35.0 2.0 35.0 108-109 22.239146086636424 33.0 2.0 35.0 2.0 35.0 110-111 22.07658640364648 33.0 2.0 35.0 2.0 35.0 112-113 21.873236577417366 32.0 2.0 35.0 2.0 35.0 114-115 21.68356376471098 32.0 2.0 35.0 2.0 35.0 116-117 21.4760239489839 32.0 2.0 35.0 2.0 35.0 118-119 21.273061182508147 32.0 2.0 35.0 2.0 35.0 120-121 21.009741454911598 32.0 2.0 35.0 2.0 35.0 122-123 20.719416866061085 31.5 2.0 35.0 2.0 35.0 124-125 19.24475709970443 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19574694157856598 1101 2 0.5904768919939087 1101 3 0.43473503165926175 1101 4 0.2496432120301364 1101 5 0.34148837384721276 1101 6 0.24306764543316461 1101 7 0.34925047429067746 1101 8 0.28827537233521383 1101 9 0.07091520631379922 1101 10-11 0.1905453768246943 1101 12-13 0.22247466658440374 1101 14-15 0.5301143898650622 1101 16-17 0.5683820939218691 1101 18-19 0.4024119624932396 1101 20-21 0.08613904883462453 1101 22-23 -0.28911086861518953 1101 24-25 0.22549671504265234 1101 26-27 -0.08217023603057783 1101 28-29 0.12110087543306491 1101 30-31 -0.4764347834705731 1101 32-33 -1.2556176045197134 1101 34-35 -0.7561342467019116 1101 36-37 -0.5976124098705284 1101 38-39 -0.6021358390668254 1101 40-41 -0.7439775497841978 1101 42-43 -0.5402645549970941 1101 44-45 -0.28514145214898434 1101 46-47 -0.10000681480424234 1101 48-49 0.49628815787795943 1101 50-51 0.35305720992123213 1101 52-53 0.22111721738200885 1101 54-55 0.2540747273573203 1101 56-57 0.14793678271876232 1101 58-59 -0.06324394753426432 1101 60-61 -0.07384244764807946 1101 62-63 0.22072189592176983 1101 64-65 0.16297503799312807 1101 66-67 0.16826545348355282 1101 68-69 0.17736703254293218 1101 70-71 0.30843007709880155 1101 72-73 0.24607299775415115 1101 74-75 0.4991549574209948 1101 76-77 0.30069173426971574 1101 78-79 0.18479987944284204 1101 80-81 0.023044880903416498 1101 82-83 -0.1839112977352002 1101 84-85 -0.5988624884442189 1101 86-87 -0.2851341438944246 1101 88-89 -0.44458910003626784 1101 90-91 -0.396877079084053 1101 92-93 -0.264192907981311 1101 94-95 0.11722230205126394 1101 96-97 -0.16854385046152132 1101 98-99 -0.12805902147396608 1101 100-101 -0.2512308389527611 1101 102-103 -0.15972648569300318 1101 104-105 -0.33920095555263075 1101 106-107 -0.7253439244008995 1101 108-109 -0.7608086091530559 1101 110-111 -0.3170985862488358 1101 112-113 -0.2692532525236686 1101 114-115 -0.44052668804245343 1101 116-117 -0.05808841415783661 1101 118-119 -0.2216557220869646 1101 120-121 -0.14442080282942982 1101 122-123 -0.14699835551504492 1101 124-125 0.11499436774940008 1102 1 0.16297483563572612 1102 2 0.5933764314788164 1102 3 0.43105578407757505 1102 4 0.42690497465775223 1102 5 0.40282947177367845 1102 6 0.34340633113770735 1102 7 0.2430030631652187 1102 8 0.4200825895082829 1102 9 0.43457593582819243 1102 10-11 0.5157993911030943 1102 12-13 0.3972937499400686 1102 14-15 0.3814033448125542 1102 16-17 0.6523773669415363 1102 18-19 0.718413034591876 1102 20-21 0.565927553013374 1102 22-23 0.6780987510334526 1102 24-25 0.3844536118044317 1102 26-27 0.5588290086271996 1102 28-29 0.3614763536134262 1102 30-31 0.2888182561727106 1102 32-33 0.389931967859134 1102 34-35 0.7771129938624028 1102 36-37 0.8031149115884944 1102 38-39 1.2204622945069161 1102 40-41 0.5960421865770016 1102 42-43 0.6099389000844155 1102 44-45 0.6511600512256877 1102 46-47 0.42110621850391183 1102 48-49 0.2040925486091787 1102 50-51 0.13981313692106312 1102 52-53 0.07914942907275702 1102 54-55 0.33899590374191035 1102 56-57 0.3752338758695153 1102 58-59 0.3549963487548098 1102 60-61 0.23016452099303564 1102 62-63 0.11570496163654553 1102 64-65 0.1917145911229241 1102 66-67 0.17673868756654443 1102 68-69 0.15526718117478921 1102 70-71 0.10564505724061135 1102 72-73 0.07855149486679736 1102 74-75 0.097373811737409 1102 76-77 0.12084109710200508 1102 78-79 -0.09284320917585731 1102 80-81 0.06545369160491177 1102 82-83 -0.195832303558646 1102 84-85 0.19955860265979908 1102 86-87 0.30888768793228394 1102 88-89 -0.30827419542868384 1102 90-91 -0.32413884171166885 1102 92-93 -0.14466000186303773 1102 94-95 0.2461726200259733 1102 96-97 0.0059645679493343096 1102 98-99 -0.19187325601990324 1102 100-101 0.014217127714719169 1102 102-103 -0.21189992201277974 1102 104-105 -0.14040584822093294 1102 106-107 0.029675446472129607 1102 108-109 -0.0029567049596863626 1102 110-111 -0.02004198431418658 1102 112-113 -0.17723551416446526 1102 114-115 -0.15557383601642982 1102 116-117 -0.06619006860095666 1102 118-119 -0.2537029431585758 1102 120-121 -0.4218324156068931 1102 122-123 -0.5286593774199702 1102 124-125 -0.43359119303128324 1103 1 0.2422534517564472 1103 2 0.7366471958022096 1103 3 0.2413858049339126 1103 4 0.2510247145669666 1103 5 0.5663554195101526 1103 6 0.17076493744367838 1103 7 0.5710977754704558 1103 8 0.3107095064509906 1103 9 0.4990342844970641 1103 10-11 0.3591253633764424 1103 12-13 0.17860279177542537 1103 14-15 0.48428029242634807 1103 16-17 0.2808217295964255 1103 18-19 0.5699649914069909 1103 20-21 0.47013562564249867 1103 22-23 0.6047273855568207 1103 24-25 0.23750845170137325 1103 26-27 0.42362473921241417 1103 28-29 0.5719597298862737 1103 30-31 -0.23704973548475294 1103 32-33 -0.014374252236564189 1103 34-35 0.2825233729569945 1103 36-37 0.4364789160051359 1103 38-39 0.3102365553338089 1103 40-41 0.3086234649477575 1103 42-43 0.3875122039735004 1103 44-45 0.16245314409773215 1103 46-47 0.061665659063351086 1103 48-49 -0.11997811756674892 1103 50-51 -0.04159405238086222 1103 52-53 -0.06206023845269826 1103 54-55 0.12977617610113157 1103 56-57 0.10938919423535864 1103 58-59 0.01125938396521775 1103 60-61 0.2871820648526864 1103 62-63 0.08012650500545959 1103 64-65 0.27855056216942486 1103 66-67 -0.0919215995147944 1103 68-69 -0.22663687967664004 1103 70-71 0.03827908987464568 1103 72-73 0.06950352529251091 1103 74-75 0.0015450790665738623 1103 76-77 -0.13395586032653384 1103 78-79 0.050372347197594536 1103 80-81 -0.10412059375885008 1103 82-83 0.1219875958402028 1103 84-85 0.1579686137013887 1103 86-87 0.045674585245500054 1103 88-89 0.18513765587790587 1103 90-91 0.28770633065981954 1103 92-93 0.15816787201746862 1103 94-95 0.09883487005664193 1103 96-97 -0.19724976158078178 1103 98-99 -0.5473633799310811 1103 100-101 -0.6097001830446978 1103 102-103 -1.0539131719207688 1103 104-105 -1.0577595471535837 1103 106-107 -1.0012348712802925 1103 108-109 -1.1029444365263643 1103 110-111 -0.7875674413133247 1103 112-113 -0.8775544934308144 1103 114-115 -0.7616037709937373 1103 116-117 -0.8462784013208662 1103 118-119 -0.7947469868342019 1103 120-121 -1.1117170923336772 1103 122-123 -1.2831741608821225 1103 124-125 -1.571991389326218 1104 1 -0.018679080917518576 1104 2 0.44577235770024615 1104 3 0.11451661854512096 1104 4 0.163517355565439 1104 5 0.4355182594960212 1104 6 0.3616397755456475 1104 7 0.2964127407359669 1104 8 0.2692636098302472 1104 9 0.5907922027827084 1104 10-11 0.2534677629996551 1104 12-13 0.3366329342456993 1104 14-15 0.3198625366274541 1104 16-17 0.38801588949280585 1104 18-19 0.7291254765123725 1104 20-21 0.5595798738469 1104 22-23 0.5872600595236221 1104 24-25 0.09771685862472168 1104 26-27 0.44607263748157067 1104 28-29 0.33176780712635434 1104 30-31 0.5370348047248328 1104 32-33 0.46116442667548085 1104 34-35 0.4113294485486563 1104 36-37 -0.16703574312590774 1104 38-39 0.025737556158020425 1104 40-41 0.09259261601334146 1104 42-43 -0.016909084146121245 1104 44-45 -0.03717007502390146 1104 46-47 -0.21383769664134533 1104 48-49 -0.3132843697744505 1104 50-51 0.1328083966948448 1104 52-53 0.06719326443203144 1104 54-55 -0.09881796024644984 1104 56-57 -0.041917764436487204 1104 58-59 0.16399104533340036 1104 60-61 -0.018157626657973935 1104 62-63 0.03744429612755695 1104 64-65 0.19178499052218356 1104 66-67 -0.2030660714328434 1104 68-69 -0.16314930167122554 1104 70-71 -0.257134780371441 1104 72-73 -0.24210603552227994 1104 74-75 0.0025223544698462774 1104 76-77 -0.09927435791277972 1104 78-79 -0.39585395681747926 1104 80-81 -0.40956625489154774 1104 82-83 -0.45235598122326337 1104 84-85 0.051027603457661996 1104 86-87 -0.3062505295549265 1104 88-89 -0.192396784249258 1104 90-91 -0.17820088704888448 1104 92-93 0.24067110997814112 1104 94-95 0.14179377739209542 1104 96-97 0.40694781289230875 1104 98-99 0.44328185732312875 1104 100-101 0.47326814381271376 1104 102-103 0.792077644045321 1104 104-105 0.7231835824825872 1104 106-107 0.7032099656442306 1104 108-109 0.8005937714095843 1104 110-111 0.7985387637967669 1104 112-113 0.7291098183553615 1104 114-115 0.4706757533204069 1104 116-117 0.1365015350973593 1104 118-119 0.08143339701132035 1104 120-121 0.4536897132709399 1104 122-123 0.6573827683537203 1104 124-125 0.5641560261924461 1105 1 0.14095981362070376 1105 2 0.34033317843556077 1105 3 0.44322879625058675 1105 4 0.23964778740056403 1105 5 0.15820372714793507 1105 6 0.2734762612076338 1105 7 0.08954540970756852 1105 8 -0.007915338489645762 1105 9 0.18852568977794704 1105 10-11 -0.18900506370135162 1105 12-13 0.25957036221668517 1105 14-15 -0.02775231434310399 1105 16-17 0.17031313487730415 1105 18-19 0.11028042645926917 1105 20-21 0.08043156751738678 1105 22-23 0.4088032317379273 1105 24-25 0.3328478101986292 1105 26-27 0.42201212181031167 1105 28-29 0.5067107488478229 1105 30-31 0.15614537349983237 1105 32-33 0.42191849984565977 1105 34-35 0.17118188793129718 1105 36-37 0.371814730288321 1105 38-39 -0.0773232532786281 1105 40-41 -0.15460531407050127 1105 42-43 0.18673247687799233 1105 44-45 0.9629316129972523 1105 46-47 1.2168845142822065 1105 48-49 1.4834243279409591 1105 50-51 1.2263839734918989 1105 52-53 1.1959585458818793 1105 54-55 1.170321985067993 1105 56-57 1.2672307678664083 1105 58-59 1.2207047144631744 1105 60-61 1.2074372482657623 1105 62-63 1.233679579611163 1105 64-65 1.2363921358004717 1105 66-67 1.1371763980042573 1105 68-69 1.2117292376368454 1105 70-71 1.094508046103602 1105 72-73 1.206044372359674 1105 74-75 1.3041855185491187 1105 76-77 1.524169250430159 1105 78-79 1.4072215408888944 1105 80-81 1.5035526297038508 1105 82-83 1.8197724620461244 1105 84-85 1.8910892941904933 1105 86-87 1.7488643779089763 1105 88-89 1.6299542427997586 1105 90-91 1.7680005004276715 1105 92-93 1.9618538546508155 1105 94-95 1.7054448292981803 1105 96-97 1.684674746659514 1105 98-99 1.7490746849280363 1105 100-101 1.7060715695691613 1105 102-103 1.7669268068139488 1105 104-105 1.4266104687953813 1105 106-107 1.4030890698857554 1105 108-109 1.3117286097256304 1105 110-111 1.0428950786228803 1105 112-113 1.3328654959365416 1105 114-115 1.4013414708988776 1105 116-117 1.5855840831731989 1105 118-119 1.5179235784679435 1105 120-121 1.7693745256000497 1105 122-123 1.7336431348825414 1105 124-125 1.3808351213950303 1106 1 0.3194109920718837 1106 2 0.6568314949338792 1106 3 0.2513096043313965 1106 4 0.13190367965646033 1106 5 0.18850675745095913 1106 6 0.23980622753759917 1106 7 0.1905555107176653 1106 8 0.17726984669554469 1106 9 0.03364353489578775 1106 10-11 0.04668517198887656 1106 12-13 0.14509224773856744 1106 14-15 0.23150694491615553 1106 16-17 -0.1007306361664746 1106 18-19 -0.3324805163016791 1106 20-21 -0.1552586681728485 1106 22-23 -0.20735838442368504 1106 24-25 0.08200605935687122 1106 26-27 0.07521077500896922 1106 28-29 -0.2003599582228901 1106 30-31 -0.17382095646649987 1106 32-33 -0.22959665166948184 1106 34-35 -0.42814471139530497 1106 36-37 0.20851506698865307 1106 38-39 0.18361950766413315 1106 40-41 -0.08389824336342855 1106 42-43 0.6867324768779852 1106 44-45 0.5740427241083665 1106 46-47 0.18826498566268057 1106 48-49 0.2426835872002151 1106 50-51 0.23816848527640744 1106 52-53 0.7144770644003948 1106 54-55 0.8252041399501486 1106 56-57 0.6763216769573184 1106 58-59 0.6752501690086312 1106 60-61 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0.18654203972158712 2216 80-81 -0.07625825498508476 2216 82-83 -0.04802279599303816 2216 84-85 0.010893609116756409 2216 86-87 -0.17537804633344933 2216 88-89 -0.2303033857505561 2216 90-91 -0.16342760173213833 2216 92-93 -0.5655866362043049 2216 94-95 -0.6911400672867174 2216 96-97 -0.8514706797298146 2216 98-99 -0.6733551887213487 2216 100-101 -1.0892099330294158 2216 102-103 -0.7172177748916084 2216 104-105 -0.38972360363625214 2216 106-107 -0.34903730419183887 2216 108-109 -0.5534915359823209 2216 110-111 -0.48260381272967123 2216 112-113 -0.1420755521752355 2216 114-115 -0.29941170546405615 2216 116-117 -0.1051267416839714 2216 118-119 -0.31573237713923064 2216 120-121 -0.6810061686830835 2216 122-123 -0.5825384252014558 2216 124-125 -0.9755979667453758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 16.0 4 67.0 5 153.0 6 432.0 7 764.0 8 1145.0 9 2011.0 10 3339.0 11 4214.0 12 4824.0 13 6285.0 14 8977.0 15 6683.0 16 2392.0 17 1888.0 18 1833.0 19 1800.0 20 1783.0 21 1906.0 22 1997.0 23 2173.0 24 2401.0 25 2601.0 26 2889.0 27 3227.0 28 3529.0 29 3866.0 30 4507.0 31 5389.0 32 6374.0 33 7714.0 34 10239.0 35 14806.0 36 19441.0 37 29917.0 38 13074.0 39 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.05666059975732 15.406157912983186 15.6152496099844 13.92193187727509 2 20.358801684657276 25.508049760185354 35.80329785736713 18.32985069779024 3 17.358682589348547 31.899137100354036 38.11212281974384 12.630057490553575 4 9.4935201325206 20.11411495945346 34.74280826737979 35.649556640646146 5 7.150247279385079 44.19665346759909 34.392147728442275 14.260951524573558 6 21.927070363673764 39.62463324058335 17.764147981334517 20.68414841440837 7 18.27030304342648 40.53625369466128 23.323733529660146 17.869709732252094 8 29.36186568214545 31.07629678550935 20.313870272728256 19.247967259616946 9 26.332514101967234 9.191992464515012 21.999068891222677 42.47642454229508 10-11 23.94113443694986 26.98239554800082 27.453634030943125 21.62283598410619 12-13 29.333715881900762 28.53658932689497 29.768143087600013 12.361551703604256 14-15 23.48207615603651 26.408843368015333 36.540064744540565 13.5690157314076 16-17 14.16990569816918 26.994575750029774 38.43909357643212 20.396424975368923 18-19 13.261262626809437 18.848997975379753 51.339822223184605 16.549917174626202 20-21 14.861470502257399 17.34298366228901 54.70345268126847 13.092093154185116 22-23 14.636272100299905 17.884055303530637 54.09660794907052 13.383064647098946 24-25 13.853491116572652 18.534478091876615 54.606823078505464 13.005207713045266 26-27 13.82398796054697 17.941166917488605 54.80495436484306 13.429890757121358 28-29 13.582549289217544 17.138085597046437 55.451317085846064 13.828048027889956 30-31 13.806394335394042 18.02940571440945 55.20338230676786 12.960817643428646 32-33 13.279397594274764 18.552342388185743 54.26983748903782 13.898422528501673 34-35 13.432165197361476 18.161099366895026 54.42916342986447 13.977572005879024 36-37 14.656843108171023 17.99394779294739 52.3859662418934 14.963242856988188 38-39 14.873380033130148 18.35935385381592 51.75584379026233 15.011422322791592 40-41 14.399164167469658 18.91639509327328 51.39530981020538 15.289130929051675 42-43 14.652717626678216 19.31734517106973 49.97076656561282 16.059170636639237 44-45 15.022318951212316 19.508035634526834 48.33995187010885 17.129693544152 46-47 15.49656074128816 20.371889304866393 45.51921843150312 18.612331522342323 48-49 16.25809385624878 21.233785217749094 42.706325659960584 19.801795266041538 50-51 16.643431983736622 21.714097000966383 41.65865859633641 19.983812418960582 52-53 16.4362648100978 22.139150630571127 40.701681252317776 20.722903307013294 54-55 16.10444310903943 22.530357906590222 40.61868953457526 20.746509449795088 56-57 17.332225347080303 22.049106889094006 39.641185689653774 20.97748207417192 58-59 16.998562701147403 21.013255089418394 40.575948116488874 21.412234092945326 60-61 17.899329803705026 21.442491960892585 40.31896579725209 20.3392124381503 62-63 19.159446415859378 20.883915690207257 40.739537741543444 19.217100152389925 64-65 18.996822269019017 21.228000844507722 41.326472610340886 18.44870427613237 66-67 18.996435174032257 21.55840007795519 40.97384440901573 18.471320338996815 68-69 19.3329489750898 21.348664355103708 40.83941674989917 18.47896991990732 70-71 18.41533989097071 21.82426470190179 40.615306326838855 19.145089080288653 72-73 18.416743133545005 21.75259378358114 40.241337588410595 19.589325494463257 74-75 17.431411186418657 22.717946774647583 40.333145665966526 19.517496372967237 76-77 16.649388684229756 22.523541814973107 41.74351534311908 19.083554157678055 78-79 16.45382891417559 22.33821984993991 41.49497092991783 19.712980305966674 80-81 15.944155127053042 22.97700377856934 40.8553749878198 20.223466106557822 82-83 15.947403180927427 22.5885906694239 40.2068468975672 21.25715925208146 84-85 15.959897252951 22.223244760468912 39.78660228284671 22.030255703733378 86-87 16.43406991977307 22.34579864231348 39.584032567153514 21.636098870759938 88-89 16.776875834012273 21.68066499029636 39.72050269455324 21.821956481138127 90-91 16.448004157610242 22.41509515676302 39.43937224416216 21.697528441464584 92-93 15.847340641494112 23.01448098524834 39.01610502097713 22.122073352280417 94-95 16.059862118993422 22.93821339345891 38.78126615581582 22.220658331731848 96-97 16.720395618316022 22.83872923585792 37.94915102842898 22.491724117397084 98-99 16.995355232671443 22.74609687966913 37.23852883220372 23.020019055455705 100-101 17.104344295574503 22.67235079171742 37.80944647448911 22.413858438218977 102-103 17.277350865748723 22.603931801141698 37.53671007365022 22.58200725945936 104-105 16.985294316671585 22.698396801723646 37.820105075396064 22.496203806208705 106-107 16.84147559486914 23.031200348625394 37.26339893517679 22.863925121328673 108-109 17.368297112727333 23.461154313585908 35.988458488181045 23.182090085505713 110-111 16.9547541196596 23.55947251033975 36.03646521296638 23.44930815703428 112-113 17.185867346662555 23.022949861131416 35.861120585576074 23.93006220662996 114-115 16.700552551999717 23.56129374595603 35.574765077414135 24.16338862463012 116-117 16.52499011873758 23.173590769547303 36.120751733958514 24.180667377756603 118-119 16.393393807980814 23.623010684576258 35.853290779105116 24.130304728337816 120-121 16.484361254347856 23.51293190953754 35.029166159134896 24.973540676979713 122-123 16.41425419897952 23.69185377671309 34.23389770866323 25.65999431564416 124-125 16.056507298904556 23.710003545916916 34.11361550892028 26.119873646258245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 239.0 1 213.0 2 665.5 3 1182.5 4 859.5 5 757.0 6 1251.0 7 3248.0 8 5028.0 9 5168.0 10 3862.0 11 2012.5 12 1457.0 13 1294.5 14 1201.5 15 1132.5 16 1167.5 17 1196.0 18 1155.0 19 1136.5 20 1183.0 21 1288.0 22 1362.5 23 1405.0 24 1509.0 25 1706.5 26 1864.0 27 2089.5 28 2376.5 29 2640.5 30 2890.5 31 3041.0 32 3376.0 33 3873.0 34 4181.0 35 4498.5 36 5135.0 37 5792.0 38 5855.0 39 5803.5 40 5630.0 41 5268.0 42 5063.5 43 4952.0 44 5137.5 45 5425.5 46 5415.0 47 5178.5 48 5490.5 49 5256.5 50 5063.5 51 4424.0 52 3017.5 53 2399.5 54 2070.5 55 1953.5 56 1979.5 57 1906.0 58 1720.0 59 1763.0 60 1791.5 61 1849.0 62 1713.5 63 1057.5 64 557.0 65 486.5 66 428.0 67 298.0 68 186.5 69 131.0 70 132.5 71 146.0 72 154.5 73 226.0 74 218.5 75 102.0 76 52.5 77 50.0 78 35.5 79 6.0 80 1.5 81 2.5 82 4.5 83 4.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06387839286294295 2 0.0 3 0.0 4 0.0 5 0.06333705055054514 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 8.120134685967325E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00324805387438693 44-45 0.009473490466961878 46-47 0.011097517404155344 48-49 0.00974416162316079 50-51 0.008932148154564057 52-53 0.006766778904972771 54-55 0.007037450061171681 56-57 0.0035187250305858406 58-59 0.0024360404057901974 60-61 0.0021653692495912863 62-63 0.001353355780994554 64-65 0.001624026937193465 66-67 0.0024360404057901974 68-69 0.001353355780994554 70-71 0.002706711561989108 72-73 0.002977382718188019 74-75 0.0010826846247956432 76-77 8.120134685967325E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.706711561989108E-4 86-87 0.0 88-89 8.120134685967325E-4 90-91 0.0024360404057901974 92-93 5.413423123978216E-4 94-95 8.120134685967325E-4 96-97 0.001353355780994554 98-99 0.0010826846247956432 100-101 5.413423123978216E-4 102-103 8.120134685967325E-4 104-105 8.120134685967325E-4 106-107 2.706711561989108E-4 108-109 8.120134685967325E-4 110-111 0.0010826846247956432 112-113 0.010285503935558611 114-115 0.02030033671491831 116-117 0.017864296309128114 118-119 0.011097517404155344 120-121 0.004601409655381484 122-123 0.004601409655381484 124-125 0.0035187250305858406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 184726.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.440760910754307 #Duplication Level Percentage of deduplicated Percentage of total 1 62.90190638782188 19.14781893182335 2 12.085645184236734 7.357924710111191 3 7.421041399914639 6.777064408908329 4 4.984706217100583 6.069530006604376 5 3.2134727557262766 4.891027792514318 6 2.180253236591265 3.9821140499983763 7 1.6254090197752171 3.4635081147212627 8 1.065229762412861 2.594112361010361 9 0.7629107981220657 2.0901226681679894 >10 3.4624413145539905 17.849680066693374 >50 0.17605633802816903 3.7612463865400647 >100 0.09958742353108552 5.468640039842794 >500 0.0071133873950775355 1.3582278618061345 >1k 0.00889173424384692 3.8890031722659506 >5k 0.0053350405463081525 11.299979428992128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7645 4.138561978281347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7058 3.820794040903825 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6171 3.340623409806957 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1862 1.0079793856847439 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1587 0.859110249775343 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.8309604495306562 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1123 0.6079274168227536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.5830256704524539 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 732 0.3962625726752054 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 648 0.3507898184337884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 570 0.30856511806675835 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 559 0.30261035263038233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 385 0.20841679027316135 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.18730444008964628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 328 0.1775602784664855 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 320 0.17322953996730292 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 316 0.17106417071771163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 313 0.16944014378051817 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 310 0.1678161168433247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 307 0.16619208990613124 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.15861329753256173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 262 0.14183168584822925 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 256 0.13858363197384233 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 246 0.13317020884986414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 217 0.1174712817903273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 208 0.1125992009787469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 206 0.11151651635395127 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 199 0.10772712016716651 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 197 0.10664443554237087 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 195 0.10556175091757522 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 186 0.10068967010599483 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.413423123978217E-4 0.0 12-13 0.0 0.0 0.0 5.413423123978217E-4 0.0 14-15 0.0 0.0 0.0 5.413423123978217E-4 0.0 16-17 0.0 0.0 0.0 5.413423123978217E-4 0.0 18-19 0.0 0.0 0.0 5.413423123978217E-4 0.0 20-21 0.0 0.0 0.0 5.413423123978217E-4 0.0 22-23 0.0 0.0 0.0 8.120134685967325E-4 0.0 24-25 0.0 0.0 0.0 0.002706711561989108 0.0 26-27 0.0 0.0 0.0 0.004872080811580394 0.0 28-29 0.0 0.0 0.0 0.008390805842166235 0.0 30-31 0.0 0.0 0.0 0.02354839058930524 0.0 32-33 0.0 0.0 0.0 0.049803492740599586 0.0 34-35 0.0 0.0 0.0 0.0828253737968667 0.0 36-37 0.0 0.0 0.0 0.1301928261316761 0.0 38-39 0.0 0.0 0.0 0.1994846421185973 0.0 40-41 0.0 0.0 0.0 0.28041531782207163 0.0 42-43 0.0 0.0 0.0 0.3670300878057231 0.0 44-45 0.0 0.0 0.0 0.4639303617249331 0.0 46-47 0.0 0.0 0.0 0.5684094280177128 0.0 48-49 0.0 0.0 0.0 0.6877754079014324 0.0 50-51 0.0 0.0 0.0 0.810389441659539 0.0 52-53 0.0 0.0 0.0 0.9292140792308609 0.0 54-55 0.0 0.0 0.0 1.0596775765187358 0.0 56-57 0.0 0.0 0.0 1.2204562433008888 0.0 58-59 0.0 0.0 0.0 1.4099260526401265 0.0 60-61 0.0 0.0 0.0 1.6015612312289553 0.0 62-63 0.0 0.0 0.0 1.778580167383043 0.0 64-65 0.0 0.0 0.0 1.9750874267834524 0.0 66-67 0.0 0.0 0.0 2.2089473057393114 0.0 68-69 0.0 0.0 0.0 2.5134523564630857 0.0 70-71 0.0 0.0 0.0 2.8263482130290267 0.0 72-73 0.0 0.0 0.0 3.1167783636304582 0.0 74-75 0.0 0.0 0.0 3.4107272392624752 0.0 76-77 0.0 0.0 0.0 3.756644976884683 0.0 78-79 0.0 0.0 0.0 4.1880947998657465 0.0 80-81 0.0 0.0 0.0 4.591665493758323 0.0 82-83 0.0 0.0 0.0 5.023385987895586 0.0 84-85 0.0 0.0 0.0 5.476489503372562 0.0 86-87 0.0 0.0 0.0 5.981020538527332 0.0 88-89 0.0 0.0 0.0 6.505581239240821 0.0 90-91 0.0 0.0 0.0 7.0255405302989296 0.0 92-93 0.0 0.0 0.0 7.575273648538918 0.0 94-95 0.0 0.0 0.0 8.157487305522775 0.0 96-97 0.0 0.0 0.0 8.788963112934834 0.0 98-99 0.0 0.0 0.0 9.45562617065275 0.0 100-101 0.0 0.0 0.0 10.200783863668352 0.0 102-103 0.0 0.0 0.0 10.919145112220262 0.0 104-105 0.0 0.0 0.0 11.629656897242402 0.0 106-107 0.0 0.0 0.0 12.372107878696013 0.0 108-109 0.0 0.0 0.0 13.10670939661986 0.0 110-111 0.0 0.0 0.0 14.001818910169657 0.0 112-113 0.0 0.0 0.0 14.798133451706853 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 15 2.690509E-4 119.08399 2 ATCACGC 25 1.7158418E-5 95.26719 3 CACGCAG 70 0.0 93.565994 5 GTGGTAC 20 8.4283436E-4 89.36141 1 TCACGCA 20 8.446521E-4 89.31299 4 TATAACG 40 1.8822902E-8 89.31299 2 TCCACGC 60 4.3655746E-11 79.38933 3 ATCCACG 65 8.913048E-11 73.282455 2 ACGCCGA 25 0.0020439634 71.48913 1 CGAGTAC 25 0.0020483641 71.45039 5 TAACGCA 50 8.8452E-8 71.45039 4 GCAGCGT 30 0.004210074 59.574272 1 CAGCGTA 30 0.004219123 59.541996 2 AGCGTAC 30 0.004219123 59.541996 3 GGTACTC 30 0.004219123 59.541996 3 TACCTGG 90 2.3646862E-11 59.541996 2 GCCTTGA 30 0.004232724 59.493637 8 GGAACGC 80 5.693437E-10 59.493633 8 GGTATCA 3285 0.0 58.39548 1 GTTATCA 180 0.0 56.26459 1 >>END_MODULE