FastQCFastQC Report
Thu 26 May 2016
SRR936212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202072
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG52710.2393654703388445No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG44020.19990263715264533No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40130.18223745635928343No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39350.17869533784544736No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38160.17329133652305648No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA33700.15303768450804514No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG29960.13605368035195942No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29690.13482756240486232No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA29170.13246615006230494No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28810.13083132613284215No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA28730.13046803192629486No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG26610.12084073545279173No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT23300.10580943765689768No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT23140.10508284924380311No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG22370.10158614250578546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.060.897141
GTACATG101700.047.969181
GTATCAA34950.047.83312
TACATGG102100.047.1402852
ACATGGG111700.041.9703373
CAACGCA42250.040.554146
TCAACGC42200.040.179255
ATCAACG43150.039.432534
AACGCAG45700.037.622797
CATGGGG80350.036.1329464
TATCAAC46950.035.6073883
ACGCAGA54000.031.0688178
CGCAGAG54850.030.2619539
CATGGGA35800.029.5805574
ATGGGGC35950.029.4571345
TAAGGTA6950.028.2487054
AGGTAAG11300.027.9040222
TTCGCGA656.306521E-527.5097522-23
GAGTACA65150.026.664771
GGTAAGG12850.026.3901063
Produced by FastQC (version 0.11.5)