##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936212_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202072 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.604665060906274 31.0 30.0 33.0 26.0 34.0 2 29.762372892439483 31.0 30.0 34.0 26.0 34.0 3 29.789978710959495 31.0 30.0 34.0 26.0 34.0 4 33.302798001155274 35.0 35.0 37.0 27.0 37.0 5 33.44125033150596 35.0 35.0 37.0 28.0 37.0 6 33.64251577605092 37.0 35.0 37.0 28.0 37.0 7 33.60149940601397 37.0 35.0 37.0 28.0 37.0 8 33.51802347970457 37.0 35.0 37.0 28.0 37.0 9 34.930566303009165 39.0 35.0 39.0 27.0 39.0 10-11 34.7582688031999 39.0 35.0 39.0 27.0 39.0 12-13 34.73910934792323 39.0 35.0 39.0 27.0 39.0 14-15 35.88681273818476 40.0 36.0 41.0 27.0 41.0 16-17 35.89025222608525 40.0 36.0 41.0 27.0 41.0 18-19 35.89740003959907 40.0 36.0 41.0 27.0 41.0 20-21 35.97957264794248 40.0 36.0 41.0 27.0 41.0 22-23 35.89429364707421 39.5 35.5 41.0 27.0 41.0 24-25 35.846052717622314 39.0 35.0 41.0 27.0 41.0 26-27 35.918675229511116 39.0 35.0 41.0 27.0 41.0 28-29 35.80492440755798 39.0 35.0 41.0 27.0 41.0 30-31 35.708248867430314 39.0 35.0 41.0 27.0 41.0 32-33 35.522413209014054 39.0 35.0 41.0 27.0 41.0 34-35 35.48756262283885 39.0 35.0 41.0 27.0 41.0 36-37 35.36194683915876 39.0 34.5 41.0 26.5 41.0 38-39 35.09470989141136 39.0 34.0 40.5 25.5 41.0 40-41 35.16383864832758 39.0 34.0 40.5 26.0 41.0 42-43 35.064353254571145 39.0 34.0 41.0 25.5 41.0 44-45 34.9325946199761 39.0 34.0 40.5 25.5 41.0 46-47 34.868938663222636 38.0 34.0 40.0 26.0 41.0 48-49 34.73372010542798 38.0 33.5 40.0 25.5 41.0 50-51 34.558988534434846 38.0 33.0 40.0 24.5 41.0 52-53 34.353154211124796 38.0 33.0 40.0 24.0 41.0 54-55 34.142006710043994 38.0 33.0 40.0 24.0 41.0 56-57 33.90192237129395 37.5 33.0 40.0 23.0 41.0 58-59 33.63582934617942 37.0 32.5 40.0 23.0 41.0 60-61 33.3523417944554 36.5 32.0 40.0 22.0 41.0 62-63 33.07064868905286 36.0 32.0 39.5 22.0 41.0 64-65 32.744280613894546 35.5 32.0 39.0 21.0 41.0 66-67 32.415482100494444 35.0 31.0 39.0 20.0 41.0 68-69 32.10438714083827 35.0 31.0 38.5 19.5 40.0 70-71 31.738683385466054 35.0 31.0 37.5 18.0 40.0 72-73 31.431678891516718 35.0 31.0 37.0 17.5 39.5 74-75 31.110488667037227 35.0 30.5 36.5 16.0 39.0 76-77 30.819050194544047 35.0 30.0 36.0 14.0 39.0 78-79 30.513243209123043 34.0 30.0 36.0 11.5 38.5 80-81 30.250974082591306 34.0 30.0 35.0 9.0 37.0 82-83 29.976861110808365 34.0 30.0 35.0 7.0 37.0 84-85 30.07330482382047 34.0 30.0 35.0 7.0 36.5 86-87 30.004969410627808 34.0 30.0 35.0 7.0 36.0 88-89 29.85472841033354 34.0 30.0 35.0 6.0 36.0 90-91 29.717498110870125 34.0 30.0 35.0 2.0 35.5 92-93 29.616119954297588 34.0 30.0 35.0 2.0 35.0 94-95 29.503615004414023 34.0 30.0 35.0 2.0 35.0 96-97 29.413973521301756 34.0 30.0 35.0 2.0 35.0 98-99 29.29259193159897 34.0 30.0 35.0 2.0 35.0 100-101 29.187980683647037 34.0 30.0 35.0 2.0 35.0 102-103 28.18477824521632 32.5 28.0 34.5 2.0 35.0 104-105 28.877587108868376 34.0 29.0 35.0 2.0 35.0 106-107 29.010448341380297 34.0 29.5 35.0 2.0 35.0 108-109 28.964697566655403 34.0 29.5 35.0 2.0 35.0 110-111 28.84374443705746 34.0 29.0 35.0 2.0 35.0 112-113 28.7949133815788 34.0 29.0 35.0 2.0 35.0 114-115 28.67041109464177 34.0 29.0 35.0 2.0 35.0 116-117 28.538160196396845 34.0 29.0 35.0 2.0 35.0 118-119 28.311308167943647 34.0 29.0 35.0 2.0 35.0 120-121 28.107972854656886 34.0 29.0 35.0 2.0 35.0 122-123 27.806154839623773 34.0 29.0 35.0 2.0 35.0 124-125 25.810228502973562 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4031493954768486 1101 2 0.5200999051122999 1101 3 0.4695653923527381 1101 4 0.5261520113206117 1101 5 0.4867683421394915 1101 6 0.44480056467092055 1101 7 0.47220260933824676 1101 8 0.40260377793779867 1101 9 0.48752117673486595 1101 10-11 0.43511681382057077 1101 12-13 0.4770897114860446 1101 14-15 0.5254770315912225 1101 16-17 0.50126602370716 1101 18-19 0.4460973019834924 1101 20-21 0.3826914897113767 1101 22-23 0.5259771242894615 1101 24-25 0.5844034046920115 1101 26-27 0.39162213567160364 1101 28-29 0.6187740627839986 1101 30-31 0.5125226198650239 1101 32-33 0.6373099994254829 1101 34-35 0.6249688263649418 1101 36-37 0.49072410658130394 1101 38-39 0.6134299175497091 1101 40-41 0.5470205189404282 1101 42-43 0.6789980731406047 1101 44-45 0.6509997592412518 1101 46-47 0.5697369491874227 1101 48-49 0.49651305779688215 1101 50-51 0.5369118843333922 1101 52-53 0.5799472335697047 1101 54-55 0.5488871950285983 1101 56-57 0.5028908398092398 1101 58-59 0.5141479730822596 1101 60-61 0.5468382363516966 1101 62-63 0.5478405498995045 1101 64-65 0.5549964990243765 1101 66-67 0.501892980669119 1101 68-69 0.46680429654061584 1101 70-71 0.431606996287762 1101 72-73 0.3559961946657495 1101 74-75 0.41962470619305847 1101 76-77 0.4500684955234888 1101 78-79 0.44540414723984867 1101 80-81 0.4806424830808851 1101 82-83 0.48682966714929776 1101 84-85 0.25561126836343817 1101 86-87 0.36119723343110266 1101 88-89 0.34925674376531646 1101 90-91 0.23591539309083842 1101 92-93 0.2927283169738075 1101 94-95 0.39223060957198186 1101 96-97 0.4334778810186819 1101 98-99 0.4278038620280675 1101 100-101 0.3406789579706029 1101 102-103 0.47014756697554816 1101 104-105 0.48149892584233456 1101 106-107 0.2845562651444169 1101 108-109 0.2915461833517199 1101 110-111 0.4422201502592529 1101 112-113 0.3868509205667543 1101 114-115 0.4636803560608733 1101 116-117 0.43720516630357054 1101 118-119 0.46113598754667606 1101 120-121 0.45482527494402447 1101 122-123 0.4065959244459094 1101 124-125 0.34628843801513653 1102 1 0.5183173615948604 1102 2 0.5207476638202984 1102 3 0.5493973216090176 1102 4 0.6408406242766205 1102 5 0.6178017512218403 1102 6 0.49096561382408055 1102 7 0.5363473778433914 1102 8 0.7456487209586342 1102 9 0.7856051869965555 1102 10-11 0.9092416615901584 1102 12-13 0.7166171799204761 1102 14-15 0.7632609753109563 1102 16-17 0.7732300157221275 1102 18-19 0.823783525288242 1102 20-21 0.7616135100757262 1102 22-23 0.9254781851941871 1102 24-25 1.006196856796457 1102 26-27 0.8007472973183241 1102 28-29 0.7792840085041775 1102 30-31 0.7183711971645934 1102 32-33 0.9558841183723672 1102 34-35 0.8596755186439751 1102 36-37 0.7922364503228039 1102 38-39 0.7362006575045896 1102 40-41 0.6899793759254464 1102 42-43 0.82012041407814 1102 44-45 0.7557768855495937 1102 46-47 0.6522357459917316 1102 48-49 0.7407730819863403 1102 50-51 0.6946347684876244 1102 52-53 0.7349564615575019 1102 54-55 0.7709032487978504 1102 56-57 0.8219942393078696 1102 58-59 0.8515196914968044 1102 60-61 0.8206188236785863 1102 62-63 0.7351519202033856 1102 64-65 0.6974785557147811 1102 66-67 0.667905276282788 1102 68-69 0.7388030661422391 1102 70-71 0.661948117959092 1102 72-73 0.6913248658773483 1102 74-75 0.6404003175210953 1102 76-77 0.6875998309242277 1102 78-79 0.7288069520459324 1102 80-81 0.682946215707787 1102 82-83 0.6186374320511376 1102 84-85 0.7598383563787685 1102 86-87 0.6937906537293266 1102 88-89 0.7635850676195588 1102 90-91 0.7718916974595906 1102 92-93 0.7026793939908877 1102 94-95 0.8226966543434173 1102 96-97 0.7659437834991465 1102 98-99 0.8033677736904892 1102 100-101 0.8094972314789572 1102 102-103 0.8214138310424772 1102 104-105 0.819740167623987 1102 106-107 0.7463229749168789 1102 108-109 0.7710615152035558 1102 110-111 0.7437500292700783 1102 112-113 0.8026747643480086 1102 114-115 0.7578088448052362 1102 116-117 0.8574285631237828 1102 118-119 0.8689837948010073 1102 120-121 0.7873571753236064 1102 122-123 0.6435827039409432 1102 124-125 0.7312680778515848 1103 1 0.13680401395074426 1103 2 0.14736476518887187 1103 3 0.17506224721651975 1103 4 0.07042667093467259 1103 5 0.05897547395413483 1103 6 0.18916934413660869 1103 7 0.2230499009436926 1103 8 0.23202266238332214 1103 9 0.35256216784671324 1103 10-11 0.40365058836732715 1103 12-13 0.1852605496637807 1103 14-15 0.2304669169012854 1103 16-17 0.2430406726741836 1103 18-19 0.3237146217397182 1103 20-21 0.24658472969144896 1103 22-23 0.3930018722739774 1103 24-25 0.427358171798609 1103 26-27 0.29029479023471083 1103 28-29 0.37529524826825167 1103 30-31 0.29752860350264854 1103 32-33 0.4562154409157273 1103 34-35 0.4459164982827275 1103 36-37 0.23656034655711267 1103 38-39 0.34683735429563 1103 40-41 0.44449499798065517 1103 42-43 0.48957893316607226 1103 44-45 0.49222289143066433 1103 46-47 0.31686987111905296 1103 48-49 0.3003059600527749 1103 50-51 0.26633311112816926 1103 52-53 0.2708431182750175 1103 54-55 0.3233835686460793 1103 56-57 0.3117904893086063 1103 58-59 0.24435303511192785 1103 60-61 0.3699399752265933 1103 62-63 0.3645184598425715 1103 64-65 0.26503691519596373 1103 66-67 0.3695815078424971 1103 68-69 0.2875101954277852 1103 70-71 0.3014039784901712 1103 72-73 0.3548449513041767 1103 74-75 0.1839919098965268 1103 76-77 0.20892747332227657 1103 78-79 0.16475983084382761 1103 80-81 0.28025011828767177 1103 82-83 0.10418847672482201 1103 84-85 0.12316014893343663 1103 86-87 0.04988283479733013 1103 88-89 0.07969838306908628 1103 90-91 0.029298171287397423 1103 92-93 0.13002013536082302 1103 94-95 0.09856249064878142 1103 96-97 0.14825482548796742 1103 98-99 0.07269507055251623 1103 100-101 0.11888752926532575 1103 102-103 0.08621148329831385 1103 104-105 0.10620828288876183 1103 106-107 0.15000052362052685 1103 108-109 0.05761469919664819 1103 110-111 0.020899491164762196 1103 112-113 0.047604901495770235 1103 114-115 0.042137216375937925 1103 116-117 -0.014556408095831586 1103 118-119 -0.14286086944651188 1103 120-121 -0.24296908382721938 1103 122-123 -0.139341036547588 1103 124-125 -0.10320569972351379 1104 1 0.1178276731739949 1104 2 0.28133210468740444 1104 3 0.27083166777816103 1104 4 0.19499574620208193 1104 5 0.10061575805417533 1104 6 0.10007916629898972 1104 7 0.08187846807070542 1104 8 0.09364267068300336 1104 9 0.23678265629987294 1104 10-11 0.29186635632072466 1104 12-13 0.2749591294621112 1104 14-15 0.36586490731953347 1104 16-17 0.399668244947776 1104 18-19 0.3174350946256368 1104 20-21 0.30296535858245477 1104 22-23 0.3483649579488386 1104 24-25 0.3621224261682059 1104 26-27 0.2918007183686413 1104 28-29 0.29052286907752034 1104 30-31 0.2842817330515359 1104 32-33 0.5475788200224443 1104 34-35 0.48321497179730244 1104 36-37 0.41071548244634215 1104 38-39 0.45990138428368255 1104 40-41 0.41788503588369963 1104 42-43 0.468045001055458 1104 44-45 0.4087486496726669 1104 46-47 0.24513124043076573 1104 48-49 0.3360494579567401 1104 50-51 0.30469190578855887 1104 52-53 0.316578141628014 1104 54-55 0.3722609145549072 1104 56-57 0.3646017840468403 1104 58-59 0.2650446727730511 1104 60-61 0.3378617739297809 1104 62-63 0.28055694206626924 1104 64-65 0.43208532761444474 1104 66-67 0.4090493299255513 1104 68-69 0.3338321251517087 1104 70-71 0.4183691594732224 1104 72-73 0.378041166374377 1104 74-75 0.3537828013109596 1104 76-77 0.29500865244067853 1104 78-79 0.2771420156060991 1104 80-81 0.2368468119759477 1104 82-83 0.2309013105728681 1104 84-85 0.17685293345251552 1104 86-87 0.09771939325104739 1104 88-89 0.23199438075234013 1104 90-91 0.22685142725082486 1104 92-93 0.13555955737682623 1104 94-95 0.11807587997016356 1104 96-97 0.07565389750971008 1104 98-99 0.07181660892022279 1104 100-101 0.06439028082876774 1104 102-103 0.11159726302737738 1104 104-105 0.10451674204308858 1104 106-107 0.09326474834838905 1104 108-109 0.06420872726118887 1104 110-111 0.045257107832711085 1104 112-113 0.01746407552773377 1104 114-115 -0.09183677651942546 1104 116-117 -0.024939929508470016 1104 118-119 0.0422371423393848 1104 120-121 -0.051745895203847425 1104 122-123 -2.015766584015921E-4 1104 124-125 0.06744680658121638 1105 1 0.10216418308163355 1105 2 0.222354861273665 1105 3 0.35957689973835016 1105 4 0.20200206312431845 1105 5 0.15850120698708992 1105 6 0.1278355659608934 1105 7 0.1354031367942099 1105 8 0.2581008273786054 1105 9 0.18425381634893512 1105 10-11 0.3212884171966195 1105 12-13 0.32954786923961166 1105 14-15 0.3531049130446746 1105 16-17 0.3328504801367629 1105 18-19 0.353612198109893 1105 20-21 0.15768049422014485 1105 22-23 0.17422681408147156 1105 24-25 0.2476110577177124 1105 26-27 0.16239046865684514 1105 28-29 0.22877447229950576 1105 30-31 0.30030431534110846 1105 32-33 0.4161756824359202 1105 34-35 0.4572062143112632 1105 36-37 0.4021987719047573 1105 38-39 0.4293449185120721 1105 40-41 0.22473087681602522 1105 42-43 0.48316685905000156 1105 44-45 0.5180752861853222 1105 46-47 0.3200185729982934 1105 48-49 0.3587872611868548 1105 50-51 0.4001674097213126 1105 52-53 0.3675867043118757 1105 54-55 0.3887572557597494 1105 56-57 0.40885952825394156 1105 58-59 0.43603649477224593 1105 60-61 0.4192727241092271 1105 62-63 0.4244718344267824 1105 64-65 0.44339320488168 1105 66-67 0.4691621265426349 1105 68-69 0.48338709443243033 1105 70-71 0.3804788220307316 1105 72-73 0.3840493989197924 1105 74-75 0.45124139149268316 1105 76-77 0.4952859687309399 1105 78-79 0.48663109663601745 1105 80-81 0.44630915384270864 1105 82-83 0.44814155006062606 1105 84-85 0.4206691533796736 1105 86-87 0.4407746953359215 1105 88-89 0.44989120613288947 1105 90-91 0.47496920752298166 1105 92-93 0.4900850582132499 1105 94-95 0.5145087033592226 1105 96-97 0.5076582971683372 1105 98-99 0.497878557655671 1105 100-101 0.5166320438746723 1105 102-103 0.5206881683900164 1105 104-105 0.53717128886964 1105 106-107 0.589667059563542 1105 108-109 0.583425286185161 1105 110-111 0.5871680803631456 1105 112-113 0.5203266833917546 1105 114-115 0.49723957814522635 1105 116-117 0.46958703131906887 1105 118-119 0.5386143958418224 1105 120-121 0.5405982968817682 1105 122-123 0.5842533319670693 1105 124-125 0.5569828944037845 1106 1 -0.08859302876557251 1106 2 -0.19161287486134881 1106 3 -0.10005746667229332 1106 4 -0.04297677655817722 1106 5 -0.004101223077896066 1106 6 0.03795767929542393 1106 7 -0.12827087551023908 1106 8 -0.10501150532965653 1106 9 0.011418574626830491 1106 10-11 0.09689794030278165 1106 12-13 0.013384589615164089 1106 14-15 -0.018956845220372998 1106 16-17 0.03580036165182321 1106 18-19 -0.0481134193603836 1106 20-21 -0.12762348064435258 1106 22-23 -0.16003572156033385 1106 24-25 -4.048661483318529 1106 26-27 -0.9657963425892646 1106 28-29 -0.6286534734478835 1106 30-31 -0.5499609110967683 1106 32-33 -5.414896028242499 1106 34-35 -1.1539265128972502 1106 36-37 -0.398390682615009 1106 38-39 -0.013933389433162802 1106 40-41 -0.6081771095180741 1106 42-43 -0.14535798746545936 1106 44-45 -0.08542892982330841 1106 46-47 -0.23630471747924275 1106 48-49 -0.026310970529870303 1106 50-51 -0.040795625548028625 1106 52-53 0.059291354123061524 1106 54-55 -0.22108851314004596 1106 56-57 -0.14109178535545652 1106 58-59 0.07430484067118925 1106 60-61 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-0.957404898606967 2216 18-19 -0.6216687267421221 2216 20-21 -0.5109411195933831 2216 22-23 -0.6671024132743426 2216 24-25 -0.5731646227260541 2216 26-27 -0.5902893728498455 2216 28-29 -0.7067390716546669 2216 30-31 -0.5950149114533758 2216 32-33 -0.6015106420890035 2216 34-35 -0.904776119038516 2216 36-37 -0.9287247302219228 2216 38-39 -0.7166463023958016 2216 40-41 -0.7921088194444579 2216 42-43 -0.5587846858021379 2216 44-45 -0.5382939575876406 2216 46-47 -0.77998379425145 2216 48-49 -0.9050361115803227 2216 50-51 -0.9171233465801478 2216 52-53 -1.154909808349231 2216 54-55 -1.0274183913208361 2216 56-57 -1.0142966157765017 2216 58-59 -0.8561157714814343 2216 60-61 -1.2401919876789123 2216 62-63 -1.256684445938241 2216 64-65 -1.0071750181870307 2216 66-67 -0.9356963249862602 2216 68-69 -0.9539798714762426 2216 70-71 -1.1211575096632416 2216 72-73 -1.1333255412161023 2216 74-75 -0.9662414683128517 2216 76-77 -0.76074361033168 2216 78-79 -0.5666775465728158 2216 80-81 -0.796956040436843 2216 82-83 -0.9058232092704372 2216 84-85 -1.057901021442909 2216 86-87 -0.879427144769263 2216 88-89 -0.743357880556033 2216 90-91 -0.7199928099945048 2216 92-93 -0.66366737702565 2216 94-95 -0.6502125300238113 2216 96-97 -0.85434889626233 2216 98-99 -0.6549221481806136 2216 100-101 -0.7313062122349905 2216 102-103 -0.7665859799523034 2216 104-105 -0.7589756519430217 2216 106-107 -0.8784353981721438 2216 108-109 -0.7661659594561101 2216 110-111 -0.7866403676465481 2216 112-113 -0.8968148649210015 2216 114-115 -0.982577638505866 2216 116-117 -0.7827055788002042 2216 118-119 -0.8814431053669374 2216 120-121 -0.7888349067331362 2216 122-123 -0.8315004854606514 2216 124-125 -0.8599866522346353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56301.0 3 9741.0 4 6337.0 5 5758.0 6 6207.0 7 6858.0 8 7473.0 9 8706.0 10 9839.0 11 11530.0 12 12596.0 13 12586.0 14 12511.0 15 11329.0 16 9831.0 17 8852.0 18 8220.0 19 8269.0 20 8279.0 21 9064.0 22 10955.0 23 14170.0 24 20501.0 25 28516.0 26 31873.0 27 33962.0 28 40792.0 29 49044.0 30 58266.0 31 71944.0 32 92133.0 33 120177.0 34 164880.0 35 241167.0 36 395694.0 37 462064.0 38 135327.0 39 320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.29905189036114 17.593977655528946 9.905970120786572 24.201000333323343 2 19.796373412289345 20.084284332468997 35.00342859218826 25.1159136630534 3 17.421463703940518 25.457398966699984 30.67435155360567 26.446785775753824 4 12.052641497866775 16.267912164263997 34.93539019057113 36.7440561472981 5 12.240918377680506 40.28047412033386 34.66331707126564 12.815290430720003 6 33.472702832802014 35.989346339336805 16.78255815009582 13.755392677765366 7 27.922886786514994 33.87017889150209 21.17538978257187 17.031544539411033 8 27.298665924327754 32.88137548256193 19.474089813623586 20.345868779486743 9 26.276235296040994 13.586337778145113 18.606584885036305 41.530842040777586 10-11 24.731187190443784 25.56364122688766 28.28016340276657 21.425008179901987 12-13 25.443607615789155 24.638646013304538 27.567442866935764 22.35030350397054 14-15 23.04166687480955 26.264354479287817 24.300969158674356 26.393009487228277 16-17 23.423091009783512 28.629012023724563 25.069419167796497 22.878477798695425 18-19 23.122459864756213 27.12591396050042 26.518245604554174 23.23338057018919 20-21 23.742353576959232 26.559120984918323 26.245543844033403 23.452981594089035 22-23 23.57053803194475 26.804124882790003 26.210223029656234 23.415114055609013 24-25 23.391818044135213 26.81568381154013 26.341734450757563 23.45076369356709 26-27 22.96885732223606 26.744556474273317 26.265501233513884 24.021084969976737 28-29 23.3320322691173 27.215061827797683 25.9863395069171 23.466566396167924 30-31 22.966957388125294 26.6797923117567 26.155780714384164 24.19746958573384 32-33 22.66701876393876 27.070747483132706 25.858804755402144 24.40342899752639 34-35 23.919993006540473 27.082421780539335 25.672081678157816 23.325503534762376 36-37 24.006221440127153 26.47831294525019 25.67427127976613 23.841194334856528 38-39 23.30700869845983 26.885981354247722 25.595324189002415 24.211685758290034 40-41 23.547274790638365 26.549180741045507 26.149008356300556 23.754536112015572 42-43 23.372254659183334 26.650042755633503 25.871404314799534 24.10629827038363 44-45 23.24456756204446 26.78063622533548 26.532685452192272 23.442110760427784 46-47 24.07928724403327 27.03152162263788 25.43718472473756 23.45200640859129 48-49 23.25934076513532 27.13299498471437 25.386114325261527 24.221549924888784 50-51 23.501957381955936 27.212343415687712 25.84459424940005 23.441104952956305 52-53 23.896914239004563 26.540405531209554 25.968211439340617 23.594468790445266 54-55 22.99363524463698 27.447329157373236 26.035189506498153 23.523846091491627 56-57 23.24071439746073 27.427106041797845 25.8156314691096 23.516548091631826 58-59 23.279691162052277 26.56817862902072 25.868220725947193 24.28390948297981 60-61 22.85599847233711 27.09601229577897 26.453294908645624 23.5946943232383 62-63 22.91794530504423 27.063288086848186 26.513958927807934 23.504807680299646 64-65 22.777059887909274 27.39669822127553 25.801325170959586 24.024916719855607 66-67 22.695136591015757 27.55032602330584 26.09190049437653 23.662636891301876 68-69 23.552900375260805 26.642540865554366 26.444545208289387 23.36001355089544 70-71 23.23627888938691 26.869394744837 25.923781472192214 23.97054489358388 72-73 23.012810236927393 27.183437197869093 25.831661797756382 23.972090767447128 74-75 23.059612414066514 27.634769431350055 26.063608887379196 23.242009267204235 76-77 23.078879280494355 27.29092091863669 26.13405356556963 23.496146235299324 78-79 23.417635868812468 27.286043262041286 26.12840469576566 23.16791617338058 80-81 23.598974780955704 27.47220110568893 25.757856485682083 23.170967627673285 82-83 23.024676781392053 27.689060747387686 25.98584513662145 23.30041733459881 84-85 22.962829250238695 27.55530881773606 26.395921105960646 23.085940826064597 86-87 23.216852700086303 27.747865245790493 25.81961006208481 23.215671992038395 88-89 23.086019427660336 27.98085343925808 25.551907719408113 23.381219413673474 90-91 22.791628511270876 27.765131845293713 25.87377464698548 23.56946499644993 92-93 22.915300526595907 27.32447408526894 26.386128857056185 23.374096531078965 94-95 22.65445481247627 27.494791259852104 26.5470684828988 23.303685444772825 96-97 23.208783573600233 27.02283992525198 26.275927368174873 23.492449132972915 98-99 22.71749471518779 27.839391661917332 25.845649424517443 23.597464198377434 100-101 22.781061058115423 27.292619748908525 26.269217180593134 23.657102012382918 102-103 22.419800023432625 26.82692735613685 26.813576209164335 23.939696411266187 104-105 22.24296285531989 27.50609087379346 26.081382702145483 24.16956356874116 106-107 22.627942408602834 27.545939833746964 25.800850565264877 24.02526719238533 108-109 22.51243267102955 27.534477927723167 25.957932137211458 23.99515726403583 110-111 22.717287226735554 27.425658696768224 26.215267410259457 23.641786666236765 112-113 22.645955514967415 27.76083283588393 26.32504513374142 23.26816651540723 114-115 22.722041066462857 28.25884527905518 25.687553657034112 23.331559997447847 116-117 22.49020914999437 27.9227685399463 26.27435451232848 23.31266779773085 118-119 22.60035262636251 28.344143348406426 25.70435015593442 23.351153869296642 120-121 22.641072450341955 28.406673750942304 25.815054993960203 23.137198804755545 122-123 22.56189658774057 28.431990953924963 25.688673333151684 23.317439125182783 124-125 22.581392259774052 28.207355133300865 25.702118577200743 23.509134029724336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 344.0 1 406.0 2 509.5 3 573.5 4 564.5 5 509.5 6 481.5 7 489.0 8 466.0 9 450.5 10 463.5 11 493.5 12 522.5 13 527.5 14 585.5 15 653.0 16 739.0 17 855.5 18 1041.5 19 1258.0 20 1600.5 21 2232.0 22 3319.5 23 4888.0 24 6431.5 25 8601.5 26 11480.0 27 14350.5 28 17606.0 29 21026.5 30 24192.5 31 29407.0 32 39486.0 33 40658.0 34 35820.0 35 36754.0 36 38920.0 37 42448.5 38 46308.0 39 49991.5 40 53044.5 41 56853.0 42 60118.5 43 65005.0 44 69600.5 45 73544.5 46 78414.5 47 80421.0 48 79252.5 49 76699.5 50 78219.0 51 79848.0 52 80413.0 53 84711.5 54 86996.0 55 83569.5 56 77955.0 57 71398.0 58 68069.5 59 63107.5 60 51409.0 61 42817.0 62 38771.5 63 29890.0 64 20456.5 65 15968.5 66 11834.5 67 8939.5 68 7243.5 69 5278.0 70 3769.5 71 2790.0 72 2068.5 73 1651.0 74 1332.0 75 1082.0 76 778.5 77 494.0 78 317.0 79 207.5 80 124.0 81 79.0 82 55.5 83 46.5 84 34.5 85 19.5 86 19.5 87 20.0 88 9.5 89 6.0 90 7.5 91 7.0 92 4.5 93 3.0 94 3.0 95 3.0 96 1.5 97 0.5 98 1.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.7247065491046616E-4 2 9.082355163682204E-5 3 4.541177581841102E-5 4 3.1788243072887717E-4 5 6.811766372761653E-4 6 5.449413098209323E-4 7 4.995295340025213E-4 8 0.0010444708438234536 9 0.0013169414987339197 10-11 0.0012942356108247143 12-13 0.0018845886964640576 14-15 0.0026792947732862506 16-17 0.0022932946788297567 18-19 0.0022024711271929347 20-21 0.0028155301007414834 22-23 0.08905249237990402 24-25 0.0019754122481008794 26-27 0.0013396473866431253 28-29 0.0012942356108247143 30-31 9.76353180095837E-4 32-33 0.0013169414987339197 34-35 6.584707493669598E-4 36-37 4.3141187027490475E-4 38-39 7.038825251853709E-4 40-41 9.536472921866315E-4 42-43 0.0010444708438234536 44-45 9.990590680050426E-4 46-47 0.0012715297229155087 48-49 1.816471032736441E-4 50-51 0.001067176731732659 52-53 9.990590680050426E-4 54-55 0.001067176731732659 56-57 9.30941404277426E-4 58-59 4.995295340025213E-4 60-61 7.038825251853709E-4 62-63 0.001021764955914248 64-65 7.492943010037819E-4 66-67 2.4976476700126066E-4 68-69 1.5894121536443859E-4 70-71 4.0870598236569924E-4 72-73 2.2705887909205513E-4 74-75 2.4976476700126066E-4 76-77 2.0435299118284962E-4 78-79 1.3623532745523308E-4 80-81 2.7247065491046616E-4 82-83 9.082355163682204E-5 84-85 2.0435299118284962E-4 86-87 1.5894121536443859E-4 88-89 2.4976476700126066E-4 90-91 1.1352943954602756E-4 92-93 1.816471032736441E-4 94-95 1.816471032736441E-4 96-97 3.1788243072887717E-4 98-99 3.1788243072887717E-4 100-101 2.7247065491046616E-4 102-103 6.357648614577543E-4 104-105 3.1788243072887717E-4 106-107 3.1788243072887717E-4 108-109 4.995295340025213E-4 110-111 7.038825251853709E-4 112-113 2.4976476700126066E-4 114-115 3.405883186380827E-4 116-117 7.265884130945764E-4 118-119 0.00111258850755107 120-121 5.449413098209323E-4 122-123 5.449413098209323E-4 124-125 2.0435299118284962E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2202072.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.70494672306059 #Duplication Level Percentage of deduplicated Percentage of total 1 50.30229176174379 12.427154380217297 2 13.117305556868175 6.481246698650697 3 9.264029137671134 6.866020388611389 4 6.683088551042564 6.6042138639601085 5 4.670400526029188 5.7690998085452625 6 3.3239986398912924 4.927152558362414 7 2.373266704255728 4.104199924177595 8 1.7880983559668562 3.533989969580273 9 1.3186714018356631 2.931993605481631 >10 6.708166471689606 28.450469456891536 >50 0.2863842979637925 4.81109293070611 >100 0.14381003413102061 6.7447671764213055 >500 0.012293136546780267 2.127013352436054 >1k 0.008009164719871993 3.979038997314176 >5k 1.8625964464818589E-4 0.24254688864422036 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5271 0.2393654703388445 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4402 0.19990263715264533 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4013 0.18223745635928343 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3935 0.17869533784544736 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3816 0.17329133652305648 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3370 0.15303768450804514 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 2996 0.13605368035195942 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2969 0.13482756240486232 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2917 0.13246615006230494 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2881 0.13083132613284215 No Hit CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA 2873 0.13046803192629486 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2661 0.12084073545279173 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2330 0.10580943765689768 No Hit CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 2314 0.10508284924380311 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2237 0.10158614250578546 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.541177581841102E-5 0.0 16-17 0.0 0.0 0.0 9.082355163682204E-5 0.0 18-19 0.0 0.0 0.0 1.3623532745523308E-4 0.0 20-21 0.0 0.0 0.0 1.3623532745523308E-4 0.0 22-23 0.0 0.0 0.0 1.5894121536443859E-4 0.0 24-25 0.0 0.0 0.0 3.632942065472882E-4 0.0 26-27 0.0 0.0 0.0 4.995295340025213E-4 0.0 28-29 0.0 0.0 0.0 6.584707493669598E-4 0.0 30-31 0.0 0.0 0.0 0.0021797652392837293 0.0 32-33 0.0 0.0 0.0 0.006857178148580065 0.0 34-35 0.0 0.0 0.0 0.012760709004973498 0.0 36-37 0.0 0.0 0.0 0.023500593986027704 0.0 38-39 0.0 0.0 0.0 0.04230106917484987 0.0 40-41 0.0 0.0 0.0 0.06269095651731642 0.0 42-43 2.270588790920551E-5 0.0 0.0 0.08912061004363163 0.0 44-45 4.541177581841102E-5 0.0 0.0 0.12388332443262527 0.0 46-47 4.541177581841102E-5 0.0 0.0 0.16411815780773745 0.0 48-49 4.541177581841102E-5 0.0 0.0 0.20973428661733132 0.0 50-51 4.541177581841102E-5 0.0 0.0 0.25814323963975744 0.0 52-53 4.541177581841102E-5 0.0 0.0 0.307369604626915 0.0 54-55 4.541177581841102E-5 0.0 0.0 0.36685903094903344 0.0 56-57 4.541177581841102E-5 0.0 0.0 0.44049422543858696 0.0 58-59 4.541177581841102E-5 0.0 0.0 0.5223489513512728 0.0 60-61 4.541177581841102E-5 0.0 0.0 0.6122869733596359 0.0 62-63 4.541177581841102E-5 0.0 0.0 0.7035873486425512 0.0 64-65 4.541177581841102E-5 0.0 0.0 0.8101914923762711 0.0 66-67 4.541177581841102E-5 0.0 0.0 0.9387068179423743 0.0 68-69 4.541177581841102E-5 0.0 0.0 1.0873622660839428 0.0 70-71 4.541177581841102E-5 0.0 0.0 1.2416033626511758 0.0 72-73 4.541177581841102E-5 0.0 0.0 1.4081737563531074 0.0 74-75 4.541177581841102E-5 0.0 0.0 1.5917735659869432 0.0 76-77 4.541177581841102E-5 0.0 0.0 1.8003498523209052 0.0 78-79 6.811766372761654E-5 0.0 0.0 2.0442565002415907 0.0 80-81 9.082355163682204E-5 0.0 0.0 2.301377974925434 0.0 82-83 9.082355163682204E-5 0.0 0.0 2.570442746649519 0.0 84-85 9.082355163682204E-5 0.0 0.0 2.8660279954515566 0.0 86-87 9.082355163682204E-5 0.0 0.0 3.183410896646431 0.0 88-89 9.082355163682204E-5 0.0 0.0 3.5371913361597622 0.0 90-91 9.082355163682204E-5 0.0 0.0 3.9199217827573305 0.0 92-93 9.082355163682204E-5 0.0 0.0 4.305876465438006 0.0 94-95 9.082355163682204E-5 0.0 0.0 4.724005391286026 0.0 96-97 9.082355163682204E-5 0.0 0.0 5.1841175038781655 0.0 98-99 9.082355163682204E-5 0.0 0.0 5.6683205635419736 0.0 100-101 9.082355163682204E-5 0.0 0.0 6.193825633312626 0.0 102-103 9.082355163682204E-5 0.0 0.0 6.722373292063112 0.0 104-105 9.082355163682204E-5 0.0 0.0 7.268881308149779 0.0 106-107 9.082355163682204E-5 0.0 0.0 7.837890859154469 0.0 108-109 9.082355163682204E-5 0.0 0.0 8.468274425177743 0.0 110-111 9.082355163682204E-5 0.0 0.0 9.123407409022048 0.0 112-113 9.082355163682204E-5 0.0 0.0 9.789461924950682 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2755 0.0 60.89714 1 GTACATG 10170 0.0 47.96918 1 GTATCAA 3495 0.0 47.8331 2 TACATGG 10210 0.0 47.140285 2 ACATGGG 11170 0.0 41.970337 3 CAACGCA 4225 0.0 40.55414 6 TCAACGC 4220 0.0 40.17925 5 ATCAACG 4315 0.0 39.43253 4 AACGCAG 4570 0.0 37.62279 7 CATGGGG 8035 0.0 36.132946 4 TATCAAC 4695 0.0 35.607388 3 ACGCAGA 5400 0.0 31.068817 8 CGCAGAG 5485 0.0 30.261953 9 CATGGGA 3580 0.0 29.580557 4 ATGGGGC 3595 0.0 29.457134 5 TAAGGTA 695 0.0 28.248705 4 AGGTAAG 1130 0.0 27.904022 2 TTCGCGA 65 6.306521E-5 27.50975 22-23 GAGTACA 6515 0.0 26.66477 1 GGTAAGG 1285 0.0 26.390106 3 >>END_MODULE