FastQCFastQC Report
Thu 26 May 2016
SRR936211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20485
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.9714425189162802No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.35147669026116674No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.33683182816695145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT580.28313400048816206No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2733707590920185No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.25872589699780324No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.22943617280937273No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.22455455211130093No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.21479131071515745No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.20990969001708568No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1952648279228704No Hit
GTACATGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA390.19038320722479862No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.19038320722479862No Hit
CTATTGGACTATGGCTCCGATTCGACTCTCAGACCAAGAGCATCTTCGAG300.1464486209421528No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.13668537954600926No Hit
GTACATGGGCGTCACCTCAGACTGGAGCATTCCCAGAAACGACACATATG270.13180375884793752No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT260.12692213814986575No Hit
GCACAGGATCATGCTGAAGATCATGCCGAAGATCATCACCACGGCGATGC250.12204051745179399No Hit
GAGTACATGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCC230.11227727605565047No Hit
CTCTTGTACAGCTCCACAGCAGCCCAGGAAACCAACCAGCATCATGAGGG230.11227727605565047No Hit
ACGCAGAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA230.11227727605565047No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG220.10739565535757872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10739565535757872No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA220.10739565535757872No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10251403465950697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGA152.6649892E-4118.982882
ATGGGCC250.002029146371.389735
GTACAAG250.002029146371.389731
GTACAAA358.9460314E-567.990221
TACAAAA551.645949E-764.899752
CGCAGAA401.7321664E-459.4914442
GAACAAA701.2578312E-859.4914441
TGGCTTG350.00769221950.9926647
GCAGAGA505.2136334E-447.5931553
GAGAACA751.4020588E-647.593151
GAAACAA909.121504E-846.2711221
ACAACAC909.121504E-846.2711225
GAAAACA1055.8789738E-945.3268131
GTACATG2400.044.618581
TACATGG2300.043.971942
CATGGGC951.3955287E-743.83584
AGAAAAC558.338831E-443.2665025
GCAGAAA558.338831E-443.2665023
CTTCCAC300.001294695839.6609636-37
TCAACAC757.916498E-539.6609573
Produced by FastQC (version 0.11.5)