FastQCFastQC Report
Thu 26 May 2016
SRR936211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20485
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6363.1047107639736393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5502.6848913839394677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4902.3919941420551623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.6639004149377593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.5613863802782524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC850.4149377593360996No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.36612155235538196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.35147669026116674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG720.35147669026116674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC590.28801562118623386No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.2782523797900903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG530.25872589699780324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.22943617280937273No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT450.2196729314132292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.20502806931901393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC420.20502806931901393No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG390.19038320722479862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC370.1806199658286551No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT360.17573834513058337No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA350.1708567244325116No Hit
GTACATGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA340.16597510373443983No Hit
CTATTGGACTATGGCTCCGATTCGACTCTCAGACCAAGAGCATCTTCGAG320.15621186233829631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC320.15621186233829631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC320.15621186233829631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.15133024164022457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC300.1464486209421528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.14156700024408103No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.13668537954600926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC280.13668537954600926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC260.12692213814986575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG260.12692213814986575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC260.12692213814986575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC250.12204051745179399No Hit
GCGTTGGACTGCTGTGGCATAGCTGGTCCTTTGGAGCAGTTTATCTCGGA240.11715889675372224No Hit
GTCCAATAGCAAGCACTGCAATGCCAGCGAGCCAGAAGATGAAGTTAAAT230.11227727605565047No Hit
ATCCTGTGCTGCGCCATCCGCAGGAGCCGAGAAATGGTCTAGAGTCTGCC230.11227727605565047No Hit
CTCTAGACCATTTCTCGGCTCCTGCGGATGGCGCAGCACAGGATCATGCT230.11227727605565047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT220.10739565535757872No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT220.10739565535757872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG210.10251403465950697No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG210.10251403465950697No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC210.10251403465950697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.065.7164151
GAAATTA150.004207439359.5118-119
CTCCCGG150.004207439359.598-99
CCCACGG150.004207439359.598-99
GCACGGC150.004207439359.590-91
CTTGCCC150.004207439359.576-77
GATAGCT150.004207439359.572-73
GTATCAA4350.050.6091962
TATCAAC6200.037.4274181
AACGCAG6400.037.18755
TCAACGC6400.037.18753
CAACGCA6400.037.18754
ATCAACG6300.036.8333322
ACGCAGA7300.032.6027376
CGCAGAG7350.032.380957
GCAGAGT7600.031.3157888
CAGAGTA7750.030.7096799
TGGAGCT400.00530268729.74999828-29
TGGTATC1306.082108E-527.4615382
GTGGTAT1357.8642384E-526.4444451