##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936211_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20485 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21913595313644 31.0 31.0 33.0 30.0 34.0 2 31.069221381498657 31.0 31.0 34.0 28.0 34.0 3 31.306028801562118 31.0 31.0 34.0 28.0 34.0 4 35.26477910666341 35.0 35.0 37.0 33.0 37.0 5 34.89982914327557 35.0 35.0 37.0 32.0 37.0 6 35.18428118135221 37.0 35.0 37.0 32.0 37.0 7 34.97754454478887 37.0 35.0 37.0 32.0 37.0 8 35.12106419331218 37.0 35.0 37.0 32.0 37.0 9 36.64281181352209 39.0 35.0 39.0 32.0 39.0 10-11 36.57263851598731 39.0 35.0 39.0 32.0 39.0 12-13 36.433683182816694 39.0 35.0 39.0 32.0 39.0 14-15 37.13749084696119 39.0 36.0 41.0 31.5 41.0 16-17 37.202636075176954 39.0 36.0 40.5 31.5 41.0 18-19 37.678057114962165 39.5 36.5 41.0 32.0 41.0 20-21 37.79582621430315 40.0 37.0 41.0 32.0 41.0 22-23 37.58144984134733 40.0 36.0 41.0 32.0 41.0 24-25 37.43380522333415 40.0 36.0 41.0 32.0 41.0 26-27 36.9599951183793 39.0 35.0 41.0 31.5 41.0 28-29 36.540248962655596 39.0 35.0 41.0 30.5 41.0 30-31 35.639223822309006 37.5 35.0 40.0 30.0 41.0 32-33 34.56743958994386 35.5 33.5 40.0 28.0 41.0 34-35 33.84315352697095 35.0 33.0 40.0 25.0 41.0 36-37 33.0918965096412 35.0 33.0 40.0 19.5 41.0 38-39 32.55377105198926 35.0 33.0 40.0 11.5 41.0 40-41 31.870319746155726 35.0 33.0 40.0 2.0 41.0 42-43 31.06719550890896 35.0 31.5 40.0 2.0 41.0 44-45 29.90275811569441 35.0 29.0 40.0 2.0 41.0 46-47 28.17154015133024 35.0 17.5 40.0 2.0 41.0 48-49 26.560214791310713 35.0 2.0 40.0 2.0 41.0 50-51 25.721894068830853 35.0 2.0 39.5 2.0 41.0 52-53 25.25562606785453 35.0 2.0 39.5 2.0 41.0 54-55 24.92162557969246 34.0 2.0 39.0 2.0 41.0 56-57 24.541957529899925 34.0 2.0 39.0 2.0 41.0 58-59 24.280058579448376 34.0 2.0 39.0 2.0 41.0 60-61 23.963412252867954 33.0 2.0 38.0 2.0 41.0 62-63 23.705589455699293 33.0 2.0 38.0 2.0 40.0 64-65 23.479155479619234 33.0 2.0 37.0 2.0 40.0 66-67 23.167073468391507 33.0 2.0 37.0 2.0 40.0 68-69 22.884012692213815 33.0 2.0 36.0 2.0 39.5 70-71 22.5286062972907 32.0 2.0 36.0 2.0 39.0 72-73 22.264315352697096 32.0 2.0 35.0 2.0 39.0 74-75 22.064949963387846 32.0 2.0 35.0 2.0 38.5 76-77 21.766536490114717 31.5 2.0 35.0 2.0 37.0 78-79 21.4992433487918 31.0 2.0 35.0 2.0 37.0 80-81 21.30234317793507 31.0 2.0 35.0 2.0 36.0 82-83 21.089431291188674 31.0 2.0 35.0 2.0 36.0 84-85 20.833121796436416 30.5 2.0 35.0 2.0 36.0 86-87 20.66790334391018 30.0 2.0 35.0 2.0 35.0 88-89 20.535904320234316 30.0 2.0 35.0 2.0 35.0 90-91 20.383475713937024 30.0 2.0 35.0 2.0 35.0 92-93 20.27612887478643 30.0 2.0 35.0 2.0 35.0 94-95 20.133585550402735 30.0 2.0 35.0 2.0 35.0 96-97 20.071442518916278 30.0 2.0 35.0 2.0 35.0 98-99 19.93612399316573 29.0 2.0 35.0 2.0 35.0 100-101 19.78901635342934 29.0 2.0 35.0 2.0 35.0 102-103 19.038320722479863 26.0 2.0 34.0 2.0 35.0 104-105 19.4878447644618 29.0 2.0 34.5 2.0 35.0 106-107 19.545399072492067 29.0 2.0 35.0 2.0 35.0 108-109 19.45799365389309 29.0 2.0 35.0 2.0 35.0 110-111 19.38659995118379 29.0 2.0 35.0 2.0 35.0 112-113 19.28091286307054 29.0 2.0 35.0 2.0 35.0 114-115 19.24752257749573 29.0 2.0 35.0 2.0 35.0 116-117 19.15582133268245 29.0 2.0 35.0 2.0 35.0 118-119 18.99399560654137 28.0 2.0 35.0 2.0 35.0 120-121 18.783963876006837 27.0 2.0 35.0 2.0 35.0 122-123 18.55784720527215 27.0 2.0 35.0 2.0 35.0 124-125 17.13736880644374 20.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.610728822813261 1101 2 0.0853137937591697 1101 3 0.5663120353098527 1101 4 0.193304896683955 1101 5 0.2848857085034453 1101 6 0.09620403550476908 1101 7 0.5924786995747979 1101 8 0.6610374407349866 1101 9 -0.34717228279721013 1101 10-11 0.23307975721601792 1101 12-13 0.4026302995896245 1101 14-15 1.1054307957670062 1101 16-17 0.8407337779244983 1101 18-19 0.3833010957107561 1101 20-21 0.5418357806031366 1101 22-23 0.3628686860677135 1101 24-25 0.7387601697900763 1101 26-27 1.7412527912282982 1101 28-29 0.9907419475178401 1101 30-31 0.2038455891034232 1101 32-33 0.83792204179138 1101 34-35 0.28964868508742825 1101 36-37 1.198892255157169 1101 38-39 1.0288671832647722 1101 40-41 1.088936598065061 1101 42-43 2.446328298643863 1101 44-45 2.3624454929820615 1101 46-47 2.507422595494276 1101 48-49 1.9028155832863867 1101 50-51 2.7548181349525045 1101 52-53 2.2270398299217966 1101 54-55 2.060807776897864 1101 56-57 2.307190894156715 1101 58-59 1.6952065545168118 1101 60-61 2.282887745175831 1101 62-63 1.2001227114642212 1101 64-65 1.2408104502037531 1101 66-67 1.213945197157571 1101 68-69 0.9717662770294453 1101 70-71 1.1578065696324416 1101 72-73 1.7390947087160633 1101 74-75 0.6493205893766998 1101 76-77 0.9995075542134124 1101 78-79 0.9232970925862958 1101 80-81 0.6560317209356015 1101 82-83 0.7530813426324556 1101 84-85 0.91509038862349 1101 86-87 1.2164730082520165 1101 88-89 1.9522056212935226 1101 90-91 1.7929458189624867 1101 92-93 1.3275430985488903 1101 94-95 1.3930909915161322 1101 96-97 1.2838637549287242 1101 98-99 1.0851273164162443 1101 100-101 1.4475725261059438 1101 102-103 1.9674824254895071 1101 104-105 1.9265658767008844 1101 106-107 1.8208514554242967 1101 108-109 1.8368825000209803 1101 110-111 2.441465644734823 1101 112-113 2.0199688823535666 1101 114-115 0.8331158863556212 1101 116-117 0.5186610996625802 1101 118-119 1.3381561142785081 1101 120-121 1.5206634617628225 1101 122-123 1.659451365948211 1101 124-125 2.2132329155008286 1102 1 0.4215292417028671 1102 2 1.1175718582753014 1102 3 0.4050217127292086 1102 4 -0.9034692968644364 1102 5 0.8977889343098937 1102 6 -0.32315080320491063 1102 7 0.9150593447360862 1102 8 0.08039227944465921 1102 9 -0.12136583118430622 1102 10-11 0.5717894346353702 1102 12-13 0.25746900926704797 1102 14-15 0.2667211183476539 1102 16-17 0.7278305521180428 1102 18-19 0.17362367635590914 1102 20-21 0.7515131999579765 1102 22-23 0.05641707316448219 1102 24-25 0.045211782693300506 1102 26-27 -0.6942310797394455 1102 28-29 -1.7834516008692631 1102 30-31 -2.9413157012191533 1102 32-33 -3.16207795820862 1102 34-35 -4.290996476202892 1102 36-37 -3.833365809358959 1102 38-39 -3.567907010283612 1102 40-41 -4.185256950322035 1102 42-43 -4.198832991678721 1102 44-45 -4.073038377985682 1102 46-47 -4.669996759344436 1102 48-49 -7.4036360296168375 1102 50-51 -6.3742141231120115 1102 52-53 -6.4181214604007835 1102 54-55 -6.2295148037473 1102 56-57 -6.015389751004577 1102 58-59 -7.175761187418676 1102 60-61 -6.410660641920945 1102 62-63 -5.638586965955131 1102 64-65 -5.630157291731734 1102 66-67 -6.269925770584365 1102 68-69 -5.237911142325395 1102 70-71 -5.067999881980462 1102 72-73 -4.502840775154905 1102 74-75 -5.108743926752336 1102 76-77 -5.565008574818847 1102 78-79 -5.157348068704028 1102 80-81 -5.876226343580527 1102 82-83 -5.5856283347869 1102 84-85 -5.52039348234425 1102 86-87 -5.896430217554435 1102 88-89 -5.773600830319381 1102 90-91 -5.142538052005257 1102 92-93 -4.769231094999498 1102 94-95 -5.284328363322578 1102 96-97 -5.103233019264822 1102 98-99 -5.5922920384224675 1102 100-101 -4.923395215829537 1102 102-103 -5.145420800316943 1102 104-105 -5.073434123299116 1102 106-107 -5.179148544575703 1102 108-109 -5.050214274172568 1102 110-111 -4.945631129458725 1102 112-113 -4.592934343452885 1102 114-115 -4.908819597515345 1102 116-117 -6.094242126143872 1102 118-119 -5.58119872443117 1102 120-121 -5.092239764043629 1102 122-123 -5.017967988890497 1102 124-125 -4.512573536112075 1103 1 1.1957227900899667 1103 2 1.4078944389204615 1103 3 0.8243765514388848 1103 4 0.935240380554923 1103 5 0.5429502246324773 1103 6 1.2897524226015449 1103 7 1.4311883769941574 1103 8 1.3707148600898265 1103 9 1.0399244913963415 1103 10-11 1.733079757216018 1103 12-13 1.9026302995896245 1103 14-15 2.3634953118960453 1103 16-17 1.6633144230857866 1103 18-19 2.0123333537752686 1103 20-21 1.7999002967321687 1103 22-23 2.0725461054225462 1103 24-25 2.125856943983628 1103 26-27 2.70899472671217 1103 28-29 3.1520322700984806 1103 30-31 3.365135911684071 1103 32-33 3.1605026869526682 1103 34-35 3.8541648141196916 1103 36-37 4.747279351931368 1103 38-39 4.141770409071228 1103 40-41 5.137323694839253 1103 42-43 5.365683137353535 1103 44-45 5.233413234917549 1103 46-47 3.6364548535587886 1103 48-49 2.8221704219960664 1103 50-51 1.0451407155976682 1103 52-53 0.9689753137927646 1103 54-55 1.351130357543024 1103 56-57 1.4200941199631636 1103 58-59 0.3081097803232602 1103 60-61 -0.039692899985464436 1103 62-63 0.651735614690029 1103 64-65 0.2569394824618172 1103 66-67 0.2462032616736991 1103 68-69 -0.8024272713576508 1103 70-71 -1.4228385916578787 1103 72-73 -0.8092923880581324 1103 74-75 -0.673260055784592 1103 76-77 -0.5972666393349755 1103 78-79 -0.8508964558008003 1103 80-81 -0.7794521500321423 1103 82-83 -0.5211122057546405 1103 84-85 -0.14942574040876622 1103 86-87 -0.23513989497378773 1103 88-89 0.1780120729064265 1103 90-91 0.2606877544463586 1103 92-93 0.5694785824198583 1103 94-95 0.4092200237741963 1103 96-97 0.2032185936384039 1103 98-99 0.2786757035130165 1103 100-101 0.41531446158981566 1103 102-103 0.693288877102411 1103 104-105 0.1039852315395926 1103 106-107 0.4660127457468768 1103 108-109 0.498172822601628 1103 110-111 0.6511430640896592 1103 112-113 1.1973882371922784 1103 114-115 1.2363416928072368 1103 116-117 1.1476933577270962 1103 118-119 1.0800915981494725 1103 120-121 1.5045344295047585 1103 122-123 1.756225559496599 1103 124-125 2.5680716251782485 1104 1 0.30862601589641514 1104 2 0.8240234711785241 1104 3 0.7598604224066285 1104 4 0.8083586601248172 1104 5 0.7590792568905442 1104 6 0.6048061860424028 1104 7 0.6774249361339386 1104 8 0.48361808589627486 1104 9 -0.2772798096789302 1104 10-11 0.7911442733450471 1104 12-13 -0.07425142084047565 1104 14-15 -0.35962296767385027 1104 16-17 -0.1985135339034585 1104 18-19 -0.13874191504194044 1104 20-21 0.31710459780743605 1104 22-23 0.2919009441322231 1104 24-25 0.3376849009728673 1104 26-27 0.4224355869272216 1104 28-29 0.485903237840418 1104 30-31 -0.008520002294424955 1104 32-33 -0.10293817326238752 1104 34-35 -0.15497497082654377 1104 36-37 -0.5403550566707906 1104 38-39 0.49768438756584743 1104 40-41 1.7539903615059202 1104 42-43 0.7345003416546128 1104 44-45 1.0769616220143163 1104 46-47 -0.060319339989597154 1104 48-49 0.14797687360897172 1104 50-51 1.2171837263503562 1104 52-53 2.203383915943302 1104 54-55 1.981775518833345 1104 56-57 1.513642507059938 1104 58-59 2.2511205330114343 1104 60-61 2.546328605390883 1104 62-63 2.8684022813566976 1104 64-65 2.0725308803112767 1104 66-67 2.58867637995327 1104 68-69 2.961551223266003 1104 70-71 3.658344204041043 1104 72-73 4.501460300113912 1104 74-75 4.335879729161643 1104 76-77 4.405959167116638 1104 78-79 3.747490640973389 1104 80-81 3.83237580695711 1104 82-83 3.6267372566109515 1104 84-85 3.8000366251826314 1104 86-87 4.221311717929435 1104 88-89 3.6274744384978206 1104 90-91 3.0090748512205536 1104 92-93 3.673242023280075 1104 94-95 4.008682389365596 1104 96-97 3.9779497764341016 1104 98-99 4.3496434454485 1104 100-101 2.434131665890895 1104 102-103 4.09328887710241 1104 104-105 3.738393833690129 1104 106-107 3.4837546812307494 1104 108-109 3.113764220451092 1104 110-111 4.12049790279934 1104 112-113 4.412442000633135 1104 114-115 4.001933090656699 1104 116-117 4.343392282458279 1104 118-119 3.8999840712677525 1104 120-121 3.1615236768165857 1104 122-123 4.665365344442833 1104 124-125 3.7885017327051322 1105 1 1.2461260158964151 1105 2 1.3615234711785256 1105 3 1.0723604224066285 1105 4 0.7104419934581472 1105 5 0.3090792568905414 1105 6 0.27563951937573705 1105 7 -0.08090839719939424 1105 8 0.13986808589627486 1105 9 0.8060535236544055 1105 10-11 0.10051927334504995 1105 12-13 1.2559569124928558 1105 14-15 0.932043698992814 1105 16-17 1.03377813276321 1105 18-19 1.0602164182913967 1105 20-21 1.1118962644741046 1105 22-23 1.226275944132226 1105 24-25 1.4741432343062044 1105 26-27 1.8057689202605545 1105 28-29 1.8702782378404166 1105 30-31 1.4810633310389107 1105 32-33 2.094978493404284 1105 34-35 2.5189833625067877 1105 36-37 3.2825616099958808 1105 38-39 2.995601054232516 1105 40-41 1.184198694839253 1105 42-43 0.7074170083212792 1105 44-45 2.0675866220143178 1105 46-47 -1.9603193399895957 1105 48-49 0.1104768736089703 1105 50-51 1.3682253930170205 1105 52-53 1.3273422492766365 1105 54-55 -0.28697448116665214 1105 56-57 -0.5426074929400606 1105 58-59 -0.35721280032189995 1105 60-61 -0.3078380612757847 1105 62-63 -1.832639385309971 1105 64-65 -0.9764274530220547 1105 66-67 -0.684240286713397 1105 68-69 -2.8467821100673305 1105 70-71 -1.9520724626256225 1105 72-73 -2.3193730332194207 1105 74-75 -0.8693286041716881 1105 76-77 -1.6002908328833634 1105 78-79 -2.771259359026608 1105 80-81 -1.6499158597095587 1105 82-83 -1.9253460767223842 1105 84-85 -1.7270467081507022 1105 86-87 -1.8818132820705635 1105 88-89 -1.9152338948355094 1105 90-91 -1.2690501487794492 1105 92-93 -2.3871746433865937 1105 94-95 -1.8559009439677396 1105 96-97 -1.632466890232564 1105 98-99 -1.4597315545514995 1105 100-101 -1.1492016674424406 1105 102-103 -2.041086122897589 1105 104-105 -1.3032728329765355 1105 106-107 -1.6599953187692513 1105 108-109 -1.703944112882244 1105 110-111 -1.3201270972006611 1105 112-113 -1.2229746660335294 1105 114-115 -0.9345252426766351 1105 116-117 -1.4732743842083877 1105 118-119 -2.016682595398912 1105 120-121 -1.9863929898500814 1105 122-123 -3.111717988890497 1105 124-125 -4.0760816006282035 1106 1 0.13215542766112165 1106 2 0.9828470005902936 1106 3 1.0833898341713315 1106 4 0.12404493463461819 1106 5 -0.6762148607565166 1106 6 0.48519834290515007 1106 7 0.39703277927119274 1106 8 0.4247945564845068 1106 9 -0.4439464763455945 1106 10-11 -1.1117969031255441 1106 12-13 -0.9232710286836152 1106 14-15 -0.29589747747777295 1106 16-17 0.679917838645558 1106 18-19 0.08870906535022272 1106 20-21 0.18083008800351763 1106 22-23 0.624253885308697 1106 24-25 0.9033711754826683 1106 26-27 1.2175336261429024 1106 28-29 0.48884441431100356 1106 30-31 0.19246038986243974 1106 32-33 0.5784343757572259 1106 34-35 0.9371818919185557 1106 36-37 0.6557233747017648 1106 38-39 0.376115760114871 1106 40-41 1.372617812486311 1106 42-43 1.6541081847918662 1106 44-45 1.9857851514260787 1106 46-47 1.8485041894221688 1106 48-49 1.4891533441972058 1106 50-51 1.1485562753699625 1106 52-53 1.230834896335459 1106 54-55 2.167069636480406 1106 56-57 2.2312895658834684 1106 58-59 2.503081317325158 1106 60-61 1.6737795857830413 1106 62-63 3.0340885558665 1106 64-65 3.5686093116838293 1106 66-67 3.07495088975719 1106 68-69 2.851747301697376 1106 70-71 3.9946187138449645 1106 72-73 3.8828328491335213 1106 74-75 3.03784051347537 1106 76-77 2.2030179906460496 1106 78-79 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sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 20.0 6 53.0 7 119.0 8 199.0 9 332.0 10 549.0 11 730.0 12 837.0 13 1084.0 14 1461.0 15 1169.0 16 381.0 17 264.0 18 200.0 19 150.0 20 184.0 21 179.0 22 164.0 23 192.0 24 204.0 25 217.0 26 223.0 27 280.0 28 332.0 29 372.0 30 454.0 31 549.0 32 644.0 33 774.0 34 960.0 35 1449.0 36 2174.0 37 2694.0 38 879.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.15670949147575 16.960578379170535 15.651409310732255 15.231302818621465 2 20.932389553331706 25.350256285086648 32.96558457407859 20.751769587503052 3 19.516719550890897 29.153038808884553 35.91896509641201 15.411276543812546 4 11.110568708811325 20.161093483036368 31.789113985843297 36.93922382230901 5 7.191362548243685 42.6352044555181 34.999267184522935 15.174165811715277 6 25.77983890651696 39.90724920673664 13.927263851598731 20.385648035147668 7 17.11496216743959 39.7412741030022 23.544056626800096 19.599707102758117 8 29.11886746399805 31.588967537222356 18.247498169392237 21.044666829387356 9 23.285330729802293 9.768123016841592 20.88845496704906 46.058091286307054 10-11 23.888210886014157 26.468147424945084 26.521845252623873 23.12179643641689 12-13 28.408591652428605 28.206004393458628 29.572858188918723 13.812545765194045 14-15 23.526970954356845 25.411276543812544 35.46741518184037 15.594337319990236 16-17 14.5032950939712 26.041005613863806 37.935074444715646 21.520624847449355 18-19 14.27629973151086 17.942396875762753 49.65828655113498 18.123016841591408 20-21 15.233097388332926 16.826946546253357 54.08347571393703 13.85648035147669 22-23 14.915792042958262 16.324139614351964 54.64486209421528 14.115206248474493 24-25 14.01025140346595 17.839882841103247 54.24701000732243 13.902855748108372 26-27 14.7278496460825 16.553575787161336 54.149377593360995 14.569196973395169 28-29 14.642421283866245 17.09787649499634 54.539907249206735 13.719794971930682 30-31 14.86453502562851 16.40224554552111 54.97193068098609 13.761288747864292 32-33 13.583109592384671 17.42738589211618 53.82719062728826 15.162313888210885 34-35 14.310471076397363 16.60483280449109 53.36831828166952 15.71637783744203 36-37 14.547229680253846 17.739809616792776 51.85257505491824 15.860385648035146 38-39 15.037832560410056 17.561630461313154 51.03246277764217 16.36807420063461 40-41 15.8164510617525 17.83744203075421 50.09030998291433 16.25579692457896 42-43 16.377837442030753 18.647791066634124 48.52575054918233 16.448620942152793 44-45 16.149583811360362 18.597895867405473 47.19652403153758 18.055996289696587 46-47 17.281780902167547 19.64215973442687 43.202011325912906 19.874048037492678 48-49 18.787834407342316 19.922866627611793 39.31849248193712 21.970806483108767 50-51 18.875707869556727 20.54042179261863 38.40802577621558 22.17584456160906 52-53 18.624292130443273 22.14167154852568 37.248584260886545 21.985452060144503 54-55 18.265475493067758 22.344268697520015 36.50165983206405 22.888595977348174 56-57 18.034027387897574 22.16662191520004 35.01110650035395 24.788244196548444 58-59 18.099934093294603 22.256938511484854 34.50826274806552 25.134864647155027 60-61 17.918426127071687 21.870194537333106 34.51634162415485 25.695037711440357 62-63 17.7617769099341 22.02587258969978 35.413717354161584 24.79863314620454 64-65 17.747565232248775 20.796211769874784 36.78879152529962 24.667431472576826 66-67 17.903780907515436 21.306353584417487 36.00527227904025 24.784593229026825 68-69 18.801562118623384 20.810349035879913 35.86038564803515 24.527703197461555 70-71 18.955795845639386 21.792086699699773 36.193219263345455 23.058898191315386 72-73 18.8162069807176 21.940444227483525 35.53819868196241 23.705150109836463 74-75 18.52086892848426 21.48889431291189 35.643153526970956 24.3470832316329 76-77 18.486697583597756 21.357090554063948 36.382719062728825 23.773492799609468 78-79 18.70392970466195 21.711008054674153 36.70490602880156 22.88015621186234 80-81 17.94483768611179 21.46204539907249 36.72199170124482 23.871125213570906 82-83 17.205272150353917 22.326092262631196 35.896997803270686 24.5716377837442 84-85 17.629973151086162 22.83866243592873 34.3129118867464 25.21845252623871 86-87 17.473761288747863 22.916768367097877 33.99804735172077 25.611422992433486 88-89 17.334635098852818 22.95582133268245 32.56285086648767 27.146692701977056 90-91 16.885938148356075 23.00519905294247 32.2805047719007 27.828358026800753 92-93 16.870881132536002 23.25115938491579 30.990969001708567 28.886990480839636 94-95 16.968513546497437 22.509153038808886 30.737124725408837 29.785208689284843 96-97 17.224798633146204 22.836221625579693 29.126189895045158 30.81278984622895 98-99 18.677080790822554 21.55967781303393 28.14010251403466 31.623138882108858 100-101 18.159628996826946 22.582377349279962 27.27361484012692 31.984378813766167 102-103 18.56724432511594 23.202343177935074 26.65853063216988 31.571881864779105 104-105 18.69660727361484 23.297534781547473 27.105198926043446 30.90065901879424 106-107 18.179155479619233 23.407371247254087 27.095435684647303 31.318037588479374 108-109 18.225530876250915 23.688064437393212 26.817183304857213 31.269221381498657 110-111 19.236026360751772 22.521357090554066 27.605565047595803 30.637051501098366 112-113 18.72726828911075 23.274830961505604 28.164132106329486 29.833768643054164 114-115 18.14208984375 24.2626953125 28.38134765625 29.213867187499996 116-117 18.696904599160238 22.749243237965043 29.00351528171077 29.550336881163947 118-119 18.155633665299746 23.45977348174185 29.310681507518062 29.073911345440344 120-121 17.757762155828942 23.26938098027729 30.235793790275338 28.737063073618437 122-123 17.647920328060927 23.81126733059949 29.977055262644015 28.563757078695566 124-125 17.6084356464644 23.210232126729966 30.381507969440307 28.799824257365326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 6.5 2 12.0 3 21.5 4 18.0 5 17.5 6 38.0 7 102.0 8 166.0 9 171.5 10 128.5 11 85.0 12 69.0 13 72.5 14 79.5 15 79.5 16 77.0 17 84.5 18 94.5 19 95.0 20 109.5 21 131.0 22 149.0 23 164.0 24 194.5 25 229.0 26 290.5 27 371.0 28 416.0 29 458.5 30 512.0 31 565.0 32 597.0 33 604.5 34 623.0 35 650.5 36 653.0 37 605.0 38 600.5 39 584.0 40 527.0 41 509.5 42 486.0 43 460.5 44 449.5 45 468.5 46 445.5 47 444.0 48 475.0 49 535.0 50 698.5 51 699.0 52 564.0 53 478.5 54 416.0 55 409.0 56 426.0 57 395.0 58 312.5 59 237.5 60 204.5 61 171.5 62 129.5 63 105.5 64 72.0 65 61.5 66 49.5 67 40.0 68 56.0 69 51.5 70 35.0 71 34.5 72 31.0 73 13.5 74 14.5 75 15.5 76 9.5 77 10.0 78 8.0 79 4.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06834268977300463 2 0.0 3 0.0 4 0.0 5 0.07810593116914816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.00732243104710764 46-47 0.00488162069807176 48-49 0.00488162069807176 50-51 0.00488162069807176 52-53 0.00488162069807176 54-55 0.00488162069807176 56-57 0.00732243104710764 58-59 0.00732243104710764 60-61 0.00244081034903588 62-63 0.0 64-65 0.00244081034903588 66-67 0.00244081034903588 68-69 0.0 70-71 0.00244081034903588 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00244081034903588 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.00732243104710764 114-115 0.0244081034903588 116-117 0.01464486209421528 118-119 0.00488162069807176 120-121 0.00488162069807176 122-123 0.00488162069807176 124-125 0.00244081034903588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 20485.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.533072980229434 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71756185878094 35.77739809616793 2 10.299738483202574 9.997559189650964 3 5.340977670488836 7.776421772028313 4 3.76181854757594 7.302904564315353 5 2.112251056125528 5.125701732975347 6 1.3377590022128345 3.8955333170612643 7 0.9454838060752363 3.2121064193312177 8 0.5532086099376383 2.1479131071515742 9 0.4224502112251056 1.8452526238711253 >10 1.388050694025347 11.20331950207469 >50 0.0704083685375176 2.3090065901879426 >100 0.030175015087507546 3.6172809372711745 >500 0.020116676725005028 5.789602147913107 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 3.1047107639736393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 550 2.6848913839394677 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 490 2.3919941420551623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.6639004149377593 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.5613863802782524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 85 0.4149377593360996 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 0.36612155235538196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.35147669026116674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 72 0.35147669026116674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 59 0.28801562118623386 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.2782523797900903 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 53 0.25872589699780324 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.22943617280937273 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 45 0.2196729314132292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 42 0.20502806931901393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 42 0.20502806931901393 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 39 0.19038320722479862 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 37 0.1806199658286551 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 36 0.17573834513058337 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35 0.1708567244325116 No Hit GTACATGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA 34 0.16597510373443983 No Hit CTATTGGACTATGGCTCCGATTCGACTCTCAGACCAAGAGCATCTTCGAG 32 0.15621186233829631 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 32 0.15621186233829631 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 32 0.15621186233829631 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 31 0.15133024164022457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 30 0.1464486209421528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 29 0.14156700024408103 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.13668537954600926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 28 0.13668537954600926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 26 0.12692213814986575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 26 0.12692213814986575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 26 0.12692213814986575 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 25 0.12204051745179399 No Hit GCGTTGGACTGCTGTGGCATAGCTGGTCCTTTGGAGCAGTTTATCTCGGA 24 0.11715889675372224 No Hit GTCCAATAGCAAGCACTGCAATGCCAGCGAGCCAGAAGATGAAGTTAAAT 23 0.11227727605565047 No Hit ATCCTGTGCTGCGCCATCCGCAGGAGCCGAGAAATGGTCTAGAGTCTGCC 23 0.11227727605565047 No Hit CTCTAGACCATTTCTCGGCTCCTGCGGATGGCGCAGCACAGGATCATGCT 23 0.11227727605565047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 22 0.10739565535757872 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 22 0.10739565535757872 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 21 0.10251403465950697 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 21 0.10251403465950697 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 21 0.10251403465950697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.00488162069807176 0.0 16-17 0.0 0.0 0.0 0.01464486209421528 0.0 18-19 0.0 0.0 0.0 0.021967293141322917 0.0 20-21 0.0 0.0 0.0 0.03905296558457408 0.0 22-23 0.0 0.0 0.0 0.051257017329753476 0.0 24-25 0.0 0.0 0.0 0.053697827678789355 0.0 26-27 0.0 0.0 0.0 0.05857944837686112 0.0 28-29 0.0 0.0 0.0 0.06590187942396875 0.0 30-31 0.0 0.0 0.0 0.08298755186721991 0.0 32-33 0.0 0.0 0.0 0.13180375884793752 0.0 34-35 0.0 0.0 0.0 0.1928240175738345 0.0 36-37 0.0 0.0 0.0 0.2953380522333415 0.0 38-39 0.0 0.0 0.0 0.41493775933609955 0.0 40-41 0.0 0.0 0.0 0.5613863802782524 0.0 42-43 0.0 0.0 0.0 0.6883085184281181 0.0 44-45 0.0 0.0 0.0 0.7786185013424457 0.0 46-47 0.0 0.0 0.0 0.9299487429826703 0.0 48-49 0.0 0.0 0.0 1.1130095191603613 0.0 50-51 0.0 0.0 0.0 1.239931657310227 0.0 52-53 0.0 0.0 0.0 1.3497681230168417 0.0 54-55 0.0 0.0 0.0 1.486453502562851 0.0 56-57 0.0 0.0 0.0 1.6963631925799365 0.0 58-59 0.0 0.0 0.0 1.8769831584085916 0.0 60-61 0.0 0.0 0.0 2.008786917256529 0.0 62-63 0.0 0.0 0.0 2.116182572614108 0.0 64-65 0.0 0.0 0.0 2.240663900414938 0.0 66-67 0.0 0.0 0.0 2.423724676592629 0.0 68-69 0.0 0.0 0.0 2.6141078838174274 0.0 70-71 0.0 0.0 0.0 2.8288991945325845 0.0 72-73 0.0 0.0 0.0 3.0510129362948497 0.0 74-75 0.0 0.0 0.0 3.3195020746887964 0.0 76-77 0.0 0.0 0.0 3.551379057847205 0.0 78-79 0.0 0.0 0.0 3.861361972174762 0.0 80-81 0.0 0.0 0.0 4.1469367830119594 0.0 82-83 0.0 0.0 0.0 4.530144007810593 0.0 84-85 0.0 0.0 0.0 4.88162069807176 0.0 86-87 0.0 0.0 0.0 5.211130095191603 0.0 88-89 0.0 0.0 0.0 5.606541371735416 0.0 90-91 0.0 0.0 0.0 5.918965096412009 0.0 92-93 0.0 0.0 0.0 6.3607517695875035 0.0 94-95 0.0 0.0 0.0 6.75372223578228 0.0 96-97 0.0 0.0 0.0 7.1540151330241635 0.0 98-99 0.0 0.0 0.0 7.5103734439834025 0.00488162069807176 100-101 0.0 0.0 0.0 7.842323651452283 0.00488162069807176 102-103 0.0 0.0 0.0 8.28166951427874 0.00488162069807176 104-105 0.0 0.0 0.0 8.786917256529168 0.00488162069807176 106-107 0.0 0.0 0.0 9.370270929948743 0.00488162069807176 108-109 0.0 0.0 0.0 9.843788137661704 0.00488162069807176 110-111 0.0 0.0 0.0 10.331950207468878 0.00488162069807176 112-113 0.0 0.0 0.0 10.907981449841348 0.00488162069807176 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 335 0.0 65.716415 1 GAAATTA 15 0.0042074393 59.5 118-119 CTCCCGG 15 0.0042074393 59.5 98-99 CCCACGG 15 0.0042074393 59.5 98-99 GCACGGC 15 0.0042074393 59.5 90-91 CTTGCCC 15 0.0042074393 59.5 76-77 GATAGCT 15 0.0042074393 59.5 72-73 GTATCAA 435 0.0 50.609196 2 TATCAAC 620 0.0 37.427418 1 AACGCAG 640 0.0 37.1875 5 TCAACGC 640 0.0 37.1875 3 CAACGCA 640 0.0 37.1875 4 ATCAACG 630 0.0 36.833332 2 ACGCAGA 730 0.0 32.602737 6 CGCAGAG 735 0.0 32.38095 7 GCAGAGT 760 0.0 31.315788 8 CAGAGTA 775 0.0 30.709679 9 TGGAGCT 40 0.005302687 29.749998 28-29 TGGTATC 130 6.082108E-5 27.461538 2 GTGGTAT 135 7.8642384E-5 26.444445 1 >>END_MODULE